Just Accepted
Articles just accepted have been peer-reviewed and accepted, which are not yet assigned to volumes /issues, but are citable by Digital Object Identifier (DOI).
, Available online , doi: 10.15886/j.cnki.rdswxb.20240129
Abstract
FullText HTML
Abstract:
The fitness cost of resistance to dimefluthrin in Aedes albopictus was analyzed by population life table comparison method using the resistant line (RR) of Ae. albopictus for experiment with the susceptible line SS as control. The results showed that the net reproduction rate (R0), mean generation time (T), intrinsic rate of increase (rm), finite rate of increase (λ), and population doubling time (D) were 41.13, 11.77 d, 0.32 d−1, 1.38 d−1, and 2.17 d, respectively, for the susceptible line population, and. 26.48, 14.27 d, 0.23 d−1, 1.26 d−1, and 3.01 d, respectively, for the resistant line. The resistant line of Ae. albopictus was longer in population doubling time than the susceptible line, indicating that its reproductive rate has slowed down. The pupation rate and emergence rate of the susceptible and resistant lines were all 100%, indicating there was no significant difference between them. The average development time from first instar larvae to pupae was 9.66±0.17 d for the susceptible line and 11.31±0.17 d for the resistant line; the average emergence time of pupae for both of the lines was 2.15±0.04 d and 2.90±0.03 d, respectively; the average survival time of female mosquitoes was 30.03±1.60 d and 21.83±1.12 d, respectively, for both of the lines, and there were all significant differences. The development time of larval and pupal stages and the life span of adult mosquitoes were prolonged, indicating that there is a certain fitness cost of resistance to dimefluthrin in Ae. albopictus.
The fitness cost of resistance to dimefluthrin in Aedes albopictus was analyzed by population life table comparison method using the resistant line (RR) of Ae. albopictus for experiment with the susceptible line SS as control. The results showed that the net reproduction rate (R0), mean generation time (T), intrinsic rate of increase (rm), finite rate of increase (λ), and population doubling time (D) were 41.13, 11.77 d, 0.32 d−1, 1.38 d−1, and 2.17 d, respectively, for the susceptible line population, and. 26.48, 14.27 d, 0.23 d−1, 1.26 d−1, and 3.01 d, respectively, for the resistant line. The resistant line of Ae. albopictus was longer in population doubling time than the susceptible line, indicating that its reproductive rate has slowed down. The pupation rate and emergence rate of the susceptible and resistant lines were all 100%, indicating there was no significant difference between them. The average development time from first instar larvae to pupae was 9.66±0.17 d for the susceptible line and 11.31±0.17 d for the resistant line; the average emergence time of pupae for both of the lines was 2.15±0.04 d and 2.90±0.03 d, respectively; the average survival time of female mosquitoes was 30.03±1.60 d and 21.83±1.12 d, respectively, for both of the lines, and there were all significant differences. The development time of larval and pupal stages and the life span of adult mosquitoes were prolonged, indicating that there is a certain fitness cost of resistance to dimefluthrin in Ae. albopictus.
, Available online , doi: 10.15886/j.cnki.rdswxb.20240158
Abstract:
Research on the central nervous system function of pests forms the foundation for developing precise behavior control technologies. Despite the significant role of neuron-labeling techniques based on genetic manipulation in this field, such techniques remain relatively scarce for non-model insects, such as the oriental fruit fly, Bactrocera dorsalis. In this context an attempt was made to identify four pan-neuronal expression genes in B. dorsalis with a view to laying the groundwork for constructing a neuron-labeling system for this species. The genomic structures of the pan-neuronal expression genes in B. dorsalis were identified and analyzed by employing bioinformatics and molecular biology to verify their full-length sequences and peripheral expression patterns. The results indicate that, by referring to four pan-neuronal expression genes from Drosophila, four homologous genes were identified in the B. dorsalis, namely BdornSyb, BdorSyt1, Bdorelav, and BdorBrp. The full genomic lengths of these four genes are 19,337 bp (5 exons, 4 introns), 26,884 bp (8 exons, 7 introns), 1,341 bp (1 exon), and 49,692 bp (14 exons, 13 introns), respectively. The domains of BdornSyb, BdorSyt1, and Bdorelav are highly conserved among closely related species. PCR cloning results indicated that the CDS sequence lengths of these four genes are all over 500 bp, consistent with the bioinformatics analysis results. Evolutionary and genomic structure analyses demonstrated that the four genes are highly conserved among Diptera insects. Expression pattern analysis revealed that all the four genes are expressed in the peripheral sensory organs of B. dorsalis, with three genes, BdornSyb, BdorSyt1 and BdorBrp, showing higher expression levels in the primary olfactory organs, the antennae, and the maxillary palp. The four genes identified are candidate pan-neuronal expression genes in B. dorsalis, providing a foundation for constructing a pan-neuronal labeling system for this species in the future.
Research on the central nervous system function of pests forms the foundation for developing precise behavior control technologies. Despite the significant role of neuron-labeling techniques based on genetic manipulation in this field, such techniques remain relatively scarce for non-model insects, such as the oriental fruit fly, Bactrocera dorsalis. In this context an attempt was made to identify four pan-neuronal expression genes in B. dorsalis with a view to laying the groundwork for constructing a neuron-labeling system for this species. The genomic structures of the pan-neuronal expression genes in B. dorsalis were identified and analyzed by employing bioinformatics and molecular biology to verify their full-length sequences and peripheral expression patterns. The results indicate that, by referring to four pan-neuronal expression genes from Drosophila, four homologous genes were identified in the B. dorsalis, namely BdornSyb, BdorSyt1, Bdorelav, and BdorBrp. The full genomic lengths of these four genes are 19,337 bp (5 exons, 4 introns), 26,884 bp (8 exons, 7 introns), 1,341 bp (1 exon), and 49,692 bp (14 exons, 13 introns), respectively. The domains of BdornSyb, BdorSyt1, and Bdorelav are highly conserved among closely related species. PCR cloning results indicated that the CDS sequence lengths of these four genes are all over 500 bp, consistent with the bioinformatics analysis results. Evolutionary and genomic structure analyses demonstrated that the four genes are highly conserved among Diptera insects. Expression pattern analysis revealed that all the four genes are expressed in the peripheral sensory organs of B. dorsalis, with three genes, BdornSyb, BdorSyt1 and BdorBrp, showing higher expression levels in the primary olfactory organs, the antennae, and the maxillary palp. The four genes identified are candidate pan-neuronal expression genes in B. dorsalis, providing a foundation for constructing a pan-neuronal labeling system for this species in the future.
, Available online , doi: 10.15886/j.cnki.rdswxb.20240073
Abstract:
Exploring the spatial ecological niche delineation between co-distributed proximate species is crucial to understand their coexistence in the community. A survey of two species of pheasants, Red Junglefowl (Gallus gallus jabouillei) and Chinese Francolin (Francolinus pintadeanus), were made at 105 and 79 distribution sites in Datian National Nature Reserve, Hainan, from March 2023 to January 2024, respectively, using the line transect method and satellite tracking technology. A maximum entropy model was used to predict the suitable habitats for these two species of pheasants by combining 12 environmental variables, and analyze their overlap. The results showed that there are differences in the distribution patterns of suitable habitats for the two species of pheasants. The suitable habitat for the Red Junglefowl was located in the north-western and central parts of the reserve, which are dominated by deciduous broad-leaved and scrub forests, while the suitable habitat of the Chinese Francolin was located in the northern part of the reserve, and was mainly in the grass area. The potential suitable habitats of the two species of pheasants were 633.18 hm2 and 337.31 hm2, accounting for 48.61% and 25.89% of the area of the reserve, respectively. The ecological overlap indices of the Red Junglefowl and the Chinese Francolin were low, with D and I values being 0.421 and 0.711, respectively, and the overlapping area of suitable habitat was 41.31 hm2, which accounted for 17.64% and 17.22% of the area of the suitable habitats for the Red Junglefowl and the Chinese Francolin, respectively. There were similarities and differences in the selection of environmental factors by the two species. Differences lie in the fact that the variables with the greatest influence on the habitat distribution of the two species of pheasants were the distance from the ranger path and the normalized difference vegetation index (NDVI), which are mainly manifested in the fact that the Red Junglefowl preferred deciduous broad-leaved forests and scrubs near the ranger paths, whereas the Chinese Francolin was more influenced by the NDVI; similarities lie in the fact that the variable with the second highest influence on the habitat distribution of the two species of pheasants was the distance from the water source, which was manifested in the fact that the Red Junglefowl and the Chinese Francolin preferred to distribute within 400 m and 500～1000 m from water sources, respectively. This survey reveals a spatial sympatric niche relationship between the Red Junglefowl and the Chinese Francolin, and helps to further understand the interspecific competition and coexistence mechanisms of tropical pheasants.
Exploring the spatial ecological niche delineation between co-distributed proximate species is crucial to understand their coexistence in the community. A survey of two species of pheasants, Red Junglefowl (Gallus gallus jabouillei) and Chinese Francolin (Francolinus pintadeanus), were made at 105 and 79 distribution sites in Datian National Nature Reserve, Hainan, from March 2023 to January 2024, respectively, using the line transect method and satellite tracking technology. A maximum entropy model was used to predict the suitable habitats for these two species of pheasants by combining 12 environmental variables, and analyze their overlap. The results showed that there are differences in the distribution patterns of suitable habitats for the two species of pheasants. The suitable habitat for the Red Junglefowl was located in the north-western and central parts of the reserve, which are dominated by deciduous broad-leaved and scrub forests, while the suitable habitat of the Chinese Francolin was located in the northern part of the reserve, and was mainly in the grass area. The potential suitable habitats of the two species of pheasants were 633.18 hm2 and 337.31 hm2, accounting for 48.61% and 25.89% of the area of the reserve, respectively. The ecological overlap indices of the Red Junglefowl and the Chinese Francolin were low, with D and I values being 0.421 and 0.711, respectively, and the overlapping area of suitable habitat was 41.31 hm2, which accounted for 17.64% and 17.22% of the area of the suitable habitats for the Red Junglefowl and the Chinese Francolin, respectively. There were similarities and differences in the selection of environmental factors by the two species. Differences lie in the fact that the variables with the greatest influence on the habitat distribution of the two species of pheasants were the distance from the ranger path and the normalized difference vegetation index (NDVI), which are mainly manifested in the fact that the Red Junglefowl preferred deciduous broad-leaved forests and scrubs near the ranger paths, whereas the Chinese Francolin was more influenced by the NDVI; similarities lie in the fact that the variable with the second highest influence on the habitat distribution of the two species of pheasants was the distance from the water source, which was manifested in the fact that the Red Junglefowl and the Chinese Francolin preferred to distribute within 400 m and 500～
, Available online , doi: 10.15886/j.cnki.rdswxb.20230143
Abstract:
The rubber tree (Hevea brasiliensis) is an important economic crop in tropical areas of China, and its related industries play an important role in the local agricultural economy. The powdery mildew fungus Erysiphe quercicola is the most severe disease infecting H. brasiliensis. The effector proteins secreted by phytopahogenic microbes play important roles in the pathogenesis, but how effector proteins promote the infection by E. quercicola is less documented, which has limited disease resistance breeding programme and disease control strategies. The E. quercicola has 24 highly conserved candidate effector proteins, which has homologous in many other powdery mildews. It is possible that these proteins are required for the pathogenicity of powdery mildew. In order to find the important virulence factor among the 24 CSEPs, two conserved effector proteins were selected for functional analysis and found that only the effector CSEP00565 suppressed hypersensitive responses induced by Phytophthora infestans INF1 when these effectors were expressed in Nicotiana benthamiana. The effector CSEP00565 suppressed callose deposition induced by fungal chitin and bacteria flg22 when this effector was expressed in Arabidopsis thaliana, further supporting that CSEP00565 has a function to inhibit plant immunity. When CSEP00565 was further silenced by using spray-induced gene, the pathogenicity of the fungus significantly decreased. And the expression level of CSEP00565 was up-regulated at the haustorium formation stage (24 h) after inoculation with E. quercicola. Further research has discovered that the signal peptide of CSEP00565 possesses the activity of direct protein secretion. All these results indicate that CSEP00565 is a key virulence factor in promoting the infection by E. quercicola, which is conducive to understanding of the molecular mechanisms underlying H. brasiliensis-E. quercicola interaction.
The rubber tree (Hevea brasiliensis) is an important economic crop in tropical areas of China, and its related industries play an important role in the local agricultural economy. The powdery mildew fungus Erysiphe quercicola is the most severe disease infecting H. brasiliensis. The effector proteins secreted by phytopahogenic microbes play important roles in the pathogenesis, but how effector proteins promote the infection by E. quercicola is less documented, which has limited disease resistance breeding programme and disease control strategies. The E. quercicola has 24 highly conserved candidate effector proteins, which has homologous in many other powdery mildews. It is possible that these proteins are required for the pathogenicity of powdery mildew. In order to find the important virulence factor among the 24 CSEPs, two conserved effector proteins were selected for functional analysis and found that only the effector CSEP00565 suppressed hypersensitive responses induced by Phytophthora infestans INF1 when these effectors were expressed in Nicotiana benthamiana. The effector CSEP00565 suppressed callose deposition induced by fungal chitin and bacteria flg22 when this effector was expressed in Arabidopsis thaliana, further supporting that CSEP00565 has a function to inhibit plant immunity. When CSEP00565 was further silenced by using spray-induced gene, the pathogenicity of the fungus significantly decreased. And the expression level of CSEP00565 was up-regulated at the haustorium formation stage (24 h) after inoculation with E. quercicola. Further research has discovered that the signal peptide of CSEP00565 possesses the activity of direct protein secretion. All these results indicate that CSEP00565 is a key virulence factor in promoting the infection by E. quercicola, which is conducive to understanding of the molecular mechanisms underlying H. brasiliensis-E. quercicola interaction.
, Available online , doi: 10.15886/j.cnki.rdswxb.20240147
Abstract:
Cassava (Manihot esculenta Crantz) has important economic value. Exploring the key genes of cassava is of great significance for improving cassava quality. To mine disease-resistant genes of cassava, a specific immune receptor-related gene resistant to Xam (MeRXam2) was cloned, and its protein structure was analyzed. The analysis was found that the coding sequence (CDS) region of this gene is3561 bp, with the protein size of about 132.847 kD, Real-time fluorescent quantitative (Real-time quantitative reverse transcription PCR, qRT-PCR) analysis showed that the expression level of MeRXam2 increased with time under pathogen infection and reached the maximum at 24 h, indicating that MeRXam2 was involved in the resistance response to cassava disease. In addition, a MeRXam2-pET32a protein expression vector was constructed by homologous recombination, and the protein was induced and determined. After exogenous spraying of MeRXam2 protein on cassava leaf tissues, the number of bacteria was found significantly lower than that of the control strain, indicating that this protein could slow down bacterial infection. This founding might provide a theoretical basis and candidate protein for cassava molecular breeding.
Cassava (Manihot esculenta Crantz) has important economic value. Exploring the key genes of cassava is of great significance for improving cassava quality. To mine disease-resistant genes of cassava, a specific immune receptor-related gene resistant to Xam (MeRXam2) was cloned, and its protein structure was analyzed. The analysis was found that the coding sequence (CDS) region of this gene is
, Available online , doi: 10.15886/j.cnki.rdswxb.20240111
Abstract:
As an important part of Hainan's agriculture, rice industry in Hainan has unique development advantages, high-quality tropical natural conditions and abundant light and heat resources. The current situation of rice industry in Hainan was analyzed in terms of planting area, production, rice varieties and branding. The analysis showed that there are major problems in rice production in Hainan, such as high cost, low yield and no premium. In order to solve these problems and promote the green and efficient development of Hainan's rice industry, a series of countermeasures are proposed, including promoting intelligent mechanised planting to reduce costs, relying on scientific and technological innovations to improve rice yield, implementing green production modes to improve the quality and efficiency of rice, as well as strengthening brand building and market promotion. The comprehensive application of these countermeasures aims to improve the market competitiveness of Hainan rice, achieve a win-win situation in terms of ecological and economic benefits, and promote the sustainable development of rice industry in Hainan.
As an important part of Hainan's agriculture, rice industry in Hainan has unique development advantages, high-quality tropical natural conditions and abundant light and heat resources. The current situation of rice industry in Hainan was analyzed in terms of planting area, production, rice varieties and branding. The analysis showed that there are major problems in rice production in Hainan, such as high cost, low yield and no premium. In order to solve these problems and promote the green and efficient development of Hainan's rice industry, a series of countermeasures are proposed, including promoting intelligent mechanised planting to reduce costs, relying on scientific and technological innovations to improve rice yield, implementing green production modes to improve the quality and efficiency of rice, as well as strengthening brand building and market promotion. The comprehensive application of these countermeasures aims to improve the market competitiveness of Hainan rice, achieve a win-win situation in terms of ecological and economic benefits, and promote the sustainable development of rice industry in Hainan.
, Available online , doi: 10.15886/j.cnki.rdswxb.20240116
Abstract:
To explore the volatile components and aroma composition of the flowers of longan (Dimocarpus longan), and to investigate the antioxidant activity of their extracts, the flowers of longan were collected from Fujian and extracted with different solvents, and their volatile components and the aroma of the components were analyzed by using the GC-MS technology. Meanwhile, DPPH and ABTS free radical scavenging assays were used to detect the antioxidant activity of the longan flower extracts. The results showed that 59 components were identified from the essential oil extracted from the longan flowers, accounting for approximately 82.2% of the total volatile components. In the essential oil 4 key aroma compounds, 3 potential aroma compounds and 1 modified aroma compound were found. The DPPH and ABTS free radical scavenging assay showed that different solvent extracts of longan flowers had a certain degree of antioxidant activity. Water extract, methanol extract and ethanol extract had significant antioxidant activity, while n-butyl alcohol extract and ethyl acetate extract had low antioxidant activity. All the results show that longan flowers have a great development potential in the fields of medicine, cosmetics, health products, functional foods and so on, which provides a scientific basis for the development and utilization of longan flowers.
To explore the volatile components and aroma composition of the flowers of longan (Dimocarpus longan), and to investigate the antioxidant activity of their extracts, the flowers of longan were collected from Fujian and extracted with different solvents, and their volatile components and the aroma of the components were analyzed by using the GC-MS technology. Meanwhile, DPPH and ABTS free radical scavenging assays were used to detect the antioxidant activity of the longan flower extracts. The results showed that 59 components were identified from the essential oil extracted from the longan flowers, accounting for approximately 82.2% of the total volatile components. In the essential oil 4 key aroma compounds, 3 potential aroma compounds and 1 modified aroma compound were found. The DPPH and ABTS free radical scavenging assay showed that different solvent extracts of longan flowers had a certain degree of antioxidant activity. Water extract, methanol extract and ethanol extract had significant antioxidant activity, while n-butyl alcohol extract and ethyl acetate extract had low antioxidant activity. All the results show that longan flowers have a great development potential in the fields of medicine, cosmetics, health products, functional foods and so on, which provides a scientific basis for the development and utilization of longan flowers.
Email alert
RSS