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, Available online , doi: 10.15886/j.cnki.rdswxb.20250170
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Abstract:
To effectively utilize climate resources and establish a long-term effective disaster prevention system to promote the healthy development of the durian industry in Hainan, the diurnal variation process of high temperatures was analyzed. The daily maximum temperature was selected as the primary meteorological factor, supplemented by relative humidity at 14:00 as secondary indicators, to characterize high temperature disasters. The percentile threshold method was used to construct classification indices for daily high temperature disasters, process-based high temperature disasters, and annual high temperature disaster types for young durian trees. The results showed that from 2015 to 2024, the number of days affected by high temperature disasters for young durian trees in major durian cultivation areas of Hainan ranged from 7.2 to 81.7 days, that the total frequency of process-based high temperature disasters ranged from 1.1 to 10.7 times, and that annual high temperature disaster types ranged from mild to severe and above. Based on comprehensive analysis of indicators including total high temperature days, days with severe and above disasters, total frequency of disaster processes, frequency of severe and above processes, and annual disaster classification, high temperature disasters were more severe in northern durian cultivation areas than in southern areas, with the distribution pattern being in the order of Chengmai > Sanya > Ledong > Baoting > Lingshui. The probability of young durian trees encountering high temperature disasters during growth in Hainan is substantial; therefore, priority should be given to implementing high temperature disaster prevention measures in cultivation practices, modifying local microclimate conditions in durian orchards, and reducing high temperature damage to durian trees.
To effectively utilize climate resources and establish a long-term effective disaster prevention system to promote the healthy development of the durian industry in Hainan, the diurnal variation process of high temperatures was analyzed. The daily maximum temperature was selected as the primary meteorological factor, supplemented by relative humidity at 14:00 as secondary indicators, to characterize high temperature disasters. The percentile threshold method was used to construct classification indices for daily high temperature disasters, process-based high temperature disasters, and annual high temperature disaster types for young durian trees. The results showed that from 2015 to 2024, the number of days affected by high temperature disasters for young durian trees in major durian cultivation areas of Hainan ranged from 7.2 to 81.7 days, that the total frequency of process-based high temperature disasters ranged from 1.1 to 10.7 times, and that annual high temperature disaster types ranged from mild to severe and above. Based on comprehensive analysis of indicators including total high temperature days, days with severe and above disasters, total frequency of disaster processes, frequency of severe and above processes, and annual disaster classification, high temperature disasters were more severe in northern durian cultivation areas than in southern areas, with the distribution pattern being in the order of Chengmai > Sanya > Ledong > Baoting > Lingshui. The probability of young durian trees encountering high temperature disasters during growth in Hainan is substantial; therefore, priority should be given to implementing high temperature disaster prevention measures in cultivation practices, modifying local microclimate conditions in durian orchards, and reducing high temperature damage to durian trees.
, Available online , doi: 10.15886/j.cnki.rdswxb.20260047
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To investigate the differences in antioxidant activities of Camellia vietnamensis seed cake from different producing areas in Hainan Province, seed cake samples were collected from five areas, namely Xisha Township (XS) and Jinbo Township (ML) in Baisha county; Zhongjiu Village (ZJ), Huishan Town in Qionghai city; Shisha Village, Changhao Township in Wuzhishan (WZS); Houchen Coffee Culture Village (HC), Chang’an Village, Fushan Town in Chengmai county, and extracted with ethanol and ethyl acetate to determine their DPPH radical scavenging capacity, ABTS+ free radical inhibitory capacity, and FRAP total reducing activity as well as their total contents of polyphenols, saponins, and polysaccharides. The results showed that the seed cakes from all the five C. vietnamensis producing areas exhibited significant antioxidant activities with obvious geographical differences. The C. vietnamensis seed cake from the HC producing area had the highest antioxidant activity, and its ethanol extract showed the optimal DPPH and ABTS+ free radical scavenging capacity and inhibitory activities, respectively, as well as the highest total phenolic and total saponin contents. The seed cake from the WZS producing area displayed outstanding FRAP total reducing activity. The seed cake from the ML producing area had the lowest antioxidant activities and contents of total phenols and total saponins. The altitude might be the key factor causing the difference between the seed cakes from the ML and HC areas where the soil is red-yellow soil. The ethyl acetate extracts had the slightly lower antioxidant activities than the ethanol extracts. Generally, the ethyl acetate extracts had higher total polyphenol contents but lower total saponin and polysaccharide contents. In summary, the antioxidant activities of the seed cakes were jointly regulated by altitude, soil type, and climatic factors, and the seed cakes from the areas with lower altitude, warm and moist climate, yellow-red soil seems to have the highest antioxidant activity. These findings provide a theoretical basis for the rational cultivation of C. vietnamensis in Hainan province, and the resource utilization of seed cake by-products to improve the camellia industrial chain, and they also lay a foundation for further research on the antioxidant mechanism of bio-active components and the synergistic or antagonistic effects among components.
To investigate the differences in antioxidant activities of Camellia vietnamensis seed cake from different producing areas in Hainan Province, seed cake samples were collected from five areas, namely Xisha Township (XS) and Jinbo Township (ML) in Baisha county; Zhongjiu Village (ZJ), Huishan Town in Qionghai city; Shisha Village, Changhao Township in Wuzhishan (WZS); Houchen Coffee Culture Village (HC), Chang’an Village, Fushan Town in Chengmai county, and extracted with ethanol and ethyl acetate to determine their DPPH radical scavenging capacity, ABTS+ free radical inhibitory capacity, and FRAP total reducing activity as well as their total contents of polyphenols, saponins, and polysaccharides. The results showed that the seed cakes from all the five C. vietnamensis producing areas exhibited significant antioxidant activities with obvious geographical differences. The C. vietnamensis seed cake from the HC producing area had the highest antioxidant activity, and its ethanol extract showed the optimal DPPH and ABTS+ free radical scavenging capacity and inhibitory activities, respectively, as well as the highest total phenolic and total saponin contents. The seed cake from the WZS producing area displayed outstanding FRAP total reducing activity. The seed cake from the ML producing area had the lowest antioxidant activities and contents of total phenols and total saponins. The altitude might be the key factor causing the difference between the seed cakes from the ML and HC areas where the soil is red-yellow soil. The ethyl acetate extracts had the slightly lower antioxidant activities than the ethanol extracts. Generally, the ethyl acetate extracts had higher total polyphenol contents but lower total saponin and polysaccharide contents. In summary, the antioxidant activities of the seed cakes were jointly regulated by altitude, soil type, and climatic factors, and the seed cakes from the areas with lower altitude, warm and moist climate, yellow-red soil seems to have the highest antioxidant activity. These findings provide a theoretical basis for the rational cultivation of C. vietnamensis in Hainan province, and the resource utilization of seed cake by-products to improve the camellia industrial chain, and they also lay a foundation for further research on the antioxidant mechanism of bio-active components and the synergistic or antagonistic effects among components.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250126
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Agarwood is a traditional precious medicinal material and a natural aromatic material, which is highly valuable due to its rich secondary metabolites. ABC transporters are a type of transmembrane proteins widely distributed in organisms and play a crucial role in the transport and accumulation of secondary metabolites in plants. To deeply explore the function of ABC transporters in the transport process of secondary metabolites in agarwood, a gene encoding an ABC transporter AsABCG1; was identified by analyzing the transcriptome data of Aquilaria sinensis. The coding sequence (CDS) length of this gene is 1986 bp, encoding a protein consisting of 661 amino acids. AsABCG1 has typical structural characteristics of plant ABC transporters, including two conserved domains, Motif A and Motif B. The promoter region of AsABCG1 contains various hormone response elements such as jasmonic acid, auxin, gibberellin, abscisic acid, as well as stress response elements such as anaerobic induction and mechanical injury. The real-time fluorescence quantitative PCR (RT-qPCR) analysis showed that methyl jasmonic acid and mechanical injury treatments could significantly induce the expression of AsABCG1 in the stems of A. sinensis. All these findings laid the foundation for further elucidating the function of the AsABCG1 gene in the defense response to stress and the accumulation of secondary metabolites in A. sinensis.
Agarwood is a traditional precious medicinal material and a natural aromatic material, which is highly valuable due to its rich secondary metabolites. ABC transporters are a type of transmembrane proteins widely distributed in organisms and play a crucial role in the transport and accumulation of secondary metabolites in plants. To deeply explore the function of ABC transporters in the transport process of secondary metabolites in agarwood, a gene encoding an ABC transporter AsABCG1; was identified by analyzing the transcriptome data of Aquilaria sinensis. The coding sequence (CDS) length of this gene is 1986 bp, encoding a protein consisting of 661 amino acids. AsABCG1 has typical structural characteristics of plant ABC transporters, including two conserved domains, Motif A and Motif B. The promoter region of AsABCG1 contains various hormone response elements such as jasmonic acid, auxin, gibberellin, abscisic acid, as well as stress response elements such as anaerobic induction and mechanical injury. The real-time fluorescence quantitative PCR (RT-qPCR) analysis showed that methyl jasmonic acid and mechanical injury treatments could significantly induce the expression of AsABCG1 in the stems of A. sinensis. All these findings laid the foundation for further elucidating the function of the AsABCG1 gene in the defense response to stress and the accumulation of secondary metabolites in A. sinensis.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250184
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To investigate the shade tolerance of Axonopus compressus, shade tolerance evaluation was performed on six accessions of superior Axonopus compressus germplasm (A03, A12, A58, A59, A69, A135) with Axonopus compressus ‘Huanan’ as the control variety, so as to provide a theoretical basis for breeding shade-tolerant varieties of A. compressus. A. compressus was treated with 75% shading or natural light under hydroponic culture and 18 indicators including chlorophyll content, leaf length, leaf width, biomass, and turf quality were measured after 28 days of treatment for comprehensive evaluation. The results show that compared to the control group, the 75% shading stress treatment, significantly increased the leaf length, but significantly decreased, leaf width, chlorophyll content, turf height, biomass, and turf quality of A. compressus. Comprehensive analysis showed that the germplasm accessions were classified into three categories: shade-tolerant, moderately shade-tolerant, and shade-sensitive. Among them, Accession A69 exhibited the highest shade tolerance and was listed in, the shade-tolerant category along with Accession A03 and the control variety; Accessions A58 and A59 were listed in the moderately shade-tolerant category; Accessions A12 and A135 were listed in the shade-sensitive category, demonstrating the poorest shade tolerance.
To investigate the shade tolerance of Axonopus compressus, shade tolerance evaluation was performed on six accessions of superior Axonopus compressus germplasm (A03, A12, A58, A59, A69, A135) with Axonopus compressus ‘Huanan’ as the control variety, so as to provide a theoretical basis for breeding shade-tolerant varieties of A. compressus. A. compressus was treated with 75% shading or natural light under hydroponic culture and 18 indicators including chlorophyll content, leaf length, leaf width, biomass, and turf quality were measured after 28 days of treatment for comprehensive evaluation. The results show that compared to the control group, the 75% shading stress treatment, significantly increased the leaf length, but significantly decreased, leaf width, chlorophyll content, turf height, biomass, and turf quality of A. compressus. Comprehensive analysis showed that the germplasm accessions were classified into three categories: shade-tolerant, moderately shade-tolerant, and shade-sensitive. Among them, Accession A69 exhibited the highest shade tolerance and was listed in, the shade-tolerant category along with Accession A03 and the control variety; Accessions A58 and A59 were listed in the moderately shade-tolerant category; Accessions A12 and A135 were listed in the shade-sensitive category, demonstrating the poorest shade tolerance.
, Available online , doi: 10.15886/j.cnki.rdswxb.20260017
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Yellow nutsedge (Cyperus esculentus) is an emerging oil crop characterized by its efficient oil accumulation in tubers. However, the regulatory mechanisms underlying oil biosynthesis remain unclear. Previous studies have demonstrated that CeWRI2 not only complements the Arabidopsis wri1-1 mutant but also significantly increases oil content when overexpressed in tobacco leaves. To dissect the molecular network by which CeWRI2 regulates oil accumulation in tubers, 17 candidate interacting proteins were identified through yeast two-hybrid screening, including CeHB1 belonging to the HD-ZIP transcription factor family. Furthermore, yeast two-hybrid assays confirmed the interaction between CeWRI2 and CeHB1. Gene expression analysis showed that CeHB1 was expressed in various tissues including leaves, leaf sheaths, flowers, flower stems, stolons, roots and tubers, with the highest expression level observed in tubers. This expression pattern was similar to that of CeWRI2 , suggesting that CeHB1 may regulate tuber development and oil biosynthesis and accumulation by modulating CeWRI2 .
Yellow nutsedge (Cyperus esculentus) is an emerging oil crop characterized by its efficient oil accumulation in tubers. However, the regulatory mechanisms underlying oil biosynthesis remain unclear. Previous studies have demonstrated that CeWRI2 not only complements the Arabidopsis wri1-1 mutant but also significantly increases oil content when overexpressed in tobacco leaves. To dissect the molecular network by which CeWRI2 regulates oil accumulation in tubers, 17 candidate interacting proteins were identified through yeast two-hybrid screening, including CeHB1 belonging to the HD-ZIP transcription factor family. Furthermore, yeast two-hybrid assays confirmed the interaction between CeWRI2 and CeHB1. Gene expression analysis showed that CeHB1 was expressed in various tissues including leaves, leaf sheaths, flowers, flower stems, stolons, roots and tubers, with the highest expression level observed in tubers. This expression pattern was similar to that of CeWRI2 , suggesting that CeHB1 may regulate tuber development and oil biosynthesis and accumulation by modulating CeWRI2 .
, Available online , doi: 10.15886/j.cnki.rdswxb.20250191
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The belowground interactions of rice varieties during the seedling stage play a critical role in population structure establishment and resource acquisition, whereas their underlying chemical ecological mechanisms remain poorly understood. Under controlled conditions, paired mixed-cultivation experiments were conducted to compare the root interaction effects between ‘Kasalath’ and three companion rice varieties (‘FL47’, ‘IRGC126961 ’, ‘IRGC 117406 ’). Root morphological traits and root exudate metabolic profiles were systematically analyzed. The results showed that under mixed cultivation, root growth of ‘Kasalath’ remained stable, whereas all the three companion varieties exhibited significant inhibition in root surface area and maximum root length, the decrease of which ranged from 30% to 46%, indicating a clear asymmetric inhibitory effect. Metabolomics analysis identified a total of 1,307 metabolites. The inhibited varieties underwent significant metabolic reprogramming after mixed cultivation, primarily involving lipid, amino acid derivative, flavonoid, and polyamine metabolism pathways. Despite its stable phenotype, ‘Kasalath’ also displayed specific metabolic profile changes across different mixed combinations, including pathways such as nucleotide metabolism, phenylpropanoid metabolism, and glutathione metabolism. Integrating phenotypic and metabolomics data, it is demonstrated that ‘Kasalath’ suppresses root growth of companion varieties through stronger rhizosphere competitive ability and metabolic homeostasis maintenance mechanisms, while the limited metabolic compensation capacity of the companion varieties leads to growth inhibition. These findings reveal the chemical basis of root interactions between rice varieties at the metabolic level, providing a theoretical foundation for mixed-cultivation design and the development of efficient plant communities.
The belowground interactions of rice varieties during the seedling stage play a critical role in population structure establishment and resource acquisition, whereas their underlying chemical ecological mechanisms remain poorly understood. Under controlled conditions, paired mixed-cultivation experiments were conducted to compare the root interaction effects between ‘Kasalath’ and three companion rice varieties (‘FL47’, ‘IRGC
, Available online , doi: 10.15886/j.cnki.rdswxb.20250106
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Seashore paspalum (Paspalum vaginatum Sw.) were treated with different concentrations of salt to observe the effect of salt treatment on the growth of seashore paspalum, and the PvMYB1 gene was cloned from seashore paspalum under different treatments and analyzed by using bioinformatics and gene expression analysis methods in a view to investigating whether MYB transcription factor genes are involved in the regulation of growth and development of seashore paspalum under salt stress. The results showed that salt stress inhibited the growth of both shoots and roots in seashore paspalum, manifested by reduction in total root length, root surface area, and root volume. The full length of the seashore paspalum PvMYB1 gene was1098 bp, encoding 365 amino acid residues, with a protein molecular weight of 39.26 kDa, and.this gene belongs to the R2R3-MYB family. Subcellular localization prediction indicated that the PvMYB1 protein was located in the nucleus. RT-qPCR analysis demonstrated that the expression of PvMYB1 was higher in leaves than in roots. The results of salt treatment at different time periods showed that 30 g·L-1 salt treatment significantly increased the transcription level of the PvMYB1 gene. Moreover, under different salt concentration treatments, the expression levels of the PvMYB1 gene in the roots and leaves were significantly upregulated when the salt concentration was 15 g·L-1. All these results revealed that PvMYB1 is implicated in the response of seashore paspalum to salt stress.
Seashore paspalum (Paspalum vaginatum Sw.) were treated with different concentrations of salt to observe the effect of salt treatment on the growth of seashore paspalum, and the PvMYB1 gene was cloned from seashore paspalum under different treatments and analyzed by using bioinformatics and gene expression analysis methods in a view to investigating whether MYB transcription factor genes are involved in the regulation of growth and development of seashore paspalum under salt stress. The results showed that salt stress inhibited the growth of both shoots and roots in seashore paspalum, manifested by reduction in total root length, root surface area, and root volume. The full length of the seashore paspalum PvMYB1 gene was
, Available online , doi: 10.15886/j.cnki.rdswxb.20250200
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The phenotypic traits of 32 accessions of Qinan agarwood germplasm of Aquilaria sinensis conserved in Danzhou City, Hainan Province were selected for evaluation and analysis to reveal their genetic diversity and variation patterns, thereby providing a theoretical basis and technical support for the identification, evaluation, development, and utilization of superior A. sinensis germplasm. A total of 23 quantitative traits and 19 qualitative traits from leaves, flowers, and fruits of the 32 accessions of Qinan agarwood germplasm were selected for observation, and evaluated by using genetic diversity indices, correlation analysis, principal component analysis, and cluster analysis. The results showed a rich phenotypic genetic diversity among the germplasm accessions. The coefficients of variation for quantitative traits ranged from 9.04% to 34.77%, with Shannon’s diversity index ranging from 1.73 to 2.09. Higher diversity indices were observed for leaf length/width ratio, calyx tube length, sepal length/width ratio, and fruit width. For qualitative traits, H′ ranged from 0.234 to 1.285, with leaf shape exhibiting the highest diversity. Correlation analysis indicated that most of the leaf, petiole, and floral traits showed highly significant positive correlations, while leaf thickness was significantly negatively correlated with floral traits and fruit stalk length. Comprehensive analysis suggested that leaf shape and young branch color could serve as ideal phenotypic indicators for identification. Eight principal components were extracted from PCA, cumulatively explaining 72.68% of the total variance. The first four principal components primarily reflected variation in petals, sepals, fruits, and leaves, contributing substantially to the phenotypic variation. Cluster analysis divided the 32 germplasm accessions into three groups: Group I (16 accessions) was characterized by rich trait variation, gray-brown young branches, relatively large and slightly curled leaves; Group II (12 accessions) showed significant individual differences in leaf and floral traits, with asymmetric capsules and fewer pericarp wrinkles; Group III (4 accessions) displayed high trait consistency, featuring relatively small, broad-elliptical leaves, capsules with a pointed apex, and an inconspicuous midline. The 32 accessions of A. sinensis germplasm possess high phenotypic diversity. Traits such as leaf shape, young branch color, and fruit shape showed good distinguishing potential and can be used for phenotypic identification. All the findings provide a basis for the classification, core germplasm screening, and breeding of A. sinensis.
The phenotypic traits of 32 accessions of Qinan agarwood germplasm of Aquilaria sinensis conserved in Danzhou City, Hainan Province were selected for evaluation and analysis to reveal their genetic diversity and variation patterns, thereby providing a theoretical basis and technical support for the identification, evaluation, development, and utilization of superior A. sinensis germplasm. A total of 23 quantitative traits and 19 qualitative traits from leaves, flowers, and fruits of the 32 accessions of Qinan agarwood germplasm were selected for observation, and evaluated by using genetic diversity indices, correlation analysis, principal component analysis, and cluster analysis. The results showed a rich phenotypic genetic diversity among the germplasm accessions. The coefficients of variation for quantitative traits ranged from 9.04% to 34.77%, with Shannon’s diversity index ranging from 1.73 to 2.09. Higher diversity indices were observed for leaf length/width ratio, calyx tube length, sepal length/width ratio, and fruit width. For qualitative traits, H′ ranged from 0.234 to 1.285, with leaf shape exhibiting the highest diversity. Correlation analysis indicated that most of the leaf, petiole, and floral traits showed highly significant positive correlations, while leaf thickness was significantly negatively correlated with floral traits and fruit stalk length. Comprehensive analysis suggested that leaf shape and young branch color could serve as ideal phenotypic indicators for identification. Eight principal components were extracted from PCA, cumulatively explaining 72.68% of the total variance. The first four principal components primarily reflected variation in petals, sepals, fruits, and leaves, contributing substantially to the phenotypic variation. Cluster analysis divided the 32 germplasm accessions into three groups: Group I (16 accessions) was characterized by rich trait variation, gray-brown young branches, relatively large and slightly curled leaves; Group II (12 accessions) showed significant individual differences in leaf and floral traits, with asymmetric capsules and fewer pericarp wrinkles; Group III (4 accessions) displayed high trait consistency, featuring relatively small, broad-elliptical leaves, capsules with a pointed apex, and an inconspicuous midline. The 32 accessions of A. sinensis germplasm possess high phenotypic diversity. Traits such as leaf shape, young branch color, and fruit shape showed good distinguishing potential and can be used for phenotypic identification. All the findings provide a basis for the classification, core germplasm screening, and breeding of A. sinensis.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250167
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To elucidate the characteristics, potential functions, and role of the late embryogenesis abundant (LEA) gene family in durian (Durio zibethinus) (DzLEAs) in response to low-temperature stress, a genome-wide identification of the LEA gene family in durian was conducted using bioinformatics approaches. Comprehensive analyses were performed, including assessments of physicochemical properties, phylogenetic relationships, chromosomal localization, subcellular localization, synteny, promoter cis-acting elements, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, as well as expression profiles under cold stress. A total of 46 DzLEAs members were identified and classified into eight subgroups: LEA1, LEA2, LEA3, LEA4, LEA5, LEA6, DHN, and SMP. These genes were distributed across 18 chromosomes. The encoded proteins ranged from 65 to 467 amino acids in length, with isoelectric points (pI) varying between 4.33 and 9.79. Most DzLEA proteins were predicted to be small hydrophilic molecules localized to multiple cellular compartments, including the nucleus, cytoplasm, mitochondria, chloroplast, plasma membrane, and extracellular space. Gene structure analysis revealed that 91% of DzLEAs possessed a relatively low number of introns (<3). Promoter analysis identified low-temperature-responsive cis-acting elements (LTRs) in 16 DzLEAs genes. Synteny analysis detected 28 and 46 pairs of homologous genes within the durian genome and between durian and Arabidopsis thaliana, respectively. GO enrichment analysis indicated that six DzLEAs were significantly enriched in the biological processes, "response to cold" and "response to water stress." Quantitative real-time PCR (qRT-PCR) validation showed that the expression patterns of six selected DzLEAs and four non-DzLEA genes were largely consistent with the transcriptomic data, with all genes being up-regulated to varying degrees under cold stress. These findings provide a valuable foundation for further exploration of the molecular mechanisms underlying the response of DzLEAs genes to low-temperature stress in durian.
To elucidate the characteristics, potential functions, and role of the late embryogenesis abundant (LEA) gene family in durian (Durio zibethinus) (DzLEAs) in response to low-temperature stress, a genome-wide identification of the LEA gene family in durian was conducted using bioinformatics approaches. Comprehensive analyses were performed, including assessments of physicochemical properties, phylogenetic relationships, chromosomal localization, subcellular localization, synteny, promoter cis-acting elements, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, as well as expression profiles under cold stress. A total of 46 DzLEAs members were identified and classified into eight subgroups: LEA1, LEA2, LEA3, LEA4, LEA5, LEA6, DHN, and SMP. These genes were distributed across 18 chromosomes. The encoded proteins ranged from 65 to 467 amino acids in length, with isoelectric points (pI) varying between 4.33 and 9.79. Most DzLEA proteins were predicted to be small hydrophilic molecules localized to multiple cellular compartments, including the nucleus, cytoplasm, mitochondria, chloroplast, plasma membrane, and extracellular space. Gene structure analysis revealed that 91% of DzLEAs possessed a relatively low number of introns (<3). Promoter analysis identified low-temperature-responsive cis-acting elements (LTRs) in 16 DzLEAs genes. Synteny analysis detected 28 and 46 pairs of homologous genes within the durian genome and between durian and Arabidopsis thaliana, respectively. GO enrichment analysis indicated that six DzLEAs were significantly enriched in the biological processes, "response to cold" and "response to water stress." Quantitative real-time PCR (qRT-PCR) validation showed that the expression patterns of six selected DzLEAs and four non-DzLEA genes were largely consistent with the transcriptomic data, with all genes being up-regulated to varying degrees under cold stress. These findings provide a valuable foundation for further exploration of the molecular mechanisms underlying the response of DzLEAs genes to low-temperature stress in durian.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250128
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To explore the potential relationship between the leaf papillae of different accessions of Paspalum vaginatum and salt tolerance, 40 accessions of Paspalum vaginatum were selected and treated with 200mmol/L NaCl salt in the experiment, and their leaf papillae traits such as the length and width of individual leaf papilla, and cluster width were measured and analyzed by using correlation analysis, principal component analysis, and cluster analysis. The results showed that there were significant differences (P<0.05) in leaf papillae traits among different accessions of P. vaginatum, and that the traits were significantly (P<0.05) positively or negatively correlated. Before and after salt stress, the length, width and area of individual leaf papilla were highly significantly positively correlated (P<0.001). After stress, the cluster width of leaf papillae and the individual width showed a significant negative correlation with the second day after rehydration (P<0.05). Principal component analysis indicates that principal components 1 and 2 collectively account for 79.75% of the variance. Variation in leaf papilla morphology primarily stems from the individual length, individual width, and area of the leaf papillae. Cluster analysis grouped the accessions of the germplasm into four. Group A exhibited significantly reduced leaf papillae following stress, with papillae area decreasing by 38.09%, and demonstrated poor salt tolerance. Group D showed enlarged leaf papillae after stress, with total papilla cluster width increasing by 14.77%, and exhibited good salt tolerance. Group B and C displayed that most of the parameters in leaf papillae were reduced except for few parameters.
To explore the potential relationship between the leaf papillae of different accessions of Paspalum vaginatum and salt tolerance, 40 accessions of Paspalum vaginatum were selected and treated with 200mmol/L NaCl salt in the experiment, and their leaf papillae traits such as the length and width of individual leaf papilla, and cluster width were measured and analyzed by using correlation analysis, principal component analysis, and cluster analysis. The results showed that there were significant differences (P<0.05) in leaf papillae traits among different accessions of P. vaginatum, and that the traits were significantly (P<0.05) positively or negatively correlated. Before and after salt stress, the length, width and area of individual leaf papilla were highly significantly positively correlated (P<0.001). After stress, the cluster width of leaf papillae and the individual width showed a significant negative correlation with the second day after rehydration (P<0.05). Principal component analysis indicates that principal components 1 and 2 collectively account for 79.75% of the variance. Variation in leaf papilla morphology primarily stems from the individual length, individual width, and area of the leaf papillae. Cluster analysis grouped the accessions of the germplasm into four. Group A exhibited significantly reduced leaf papillae following stress, with papillae area decreasing by 38.09%, and demonstrated poor salt tolerance. Group D showed enlarged leaf papillae after stress, with total papilla cluster width increasing by 14.77%, and exhibited good salt tolerance. Group B and C displayed that most of the parameters in leaf papillae were reduced except for few parameters.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250142
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A field survey of Rhynchostylis gigantea was made in fragmented fengshui forest landscapes in Changjiang, Hainan and samples of R. gigantea was collected to analyzed its genetic diversity and population structure by using SNP molecular markers. The results showed that all three local populations (B, M, and J) of R. gigantea maintained moderate levels of genetic diversity (Pi = 0.137–0.147, He = 0.132–0.145), whereas the observed heterozygosity was relatively low (Ho = 0.105–0.122), indicating mild to moderate inbreeding (Fis = 0.049–0.107). The genetic differentiation among the populations was moderate to low (Fst = 0.026–0.058), with the greatest divergence observed between the populations M and J. STRUCTURE, PCA, and UPGMA analyses consistently revealed moderate genetic differentiation among the three populations occurring in human-influenced environments, suggesting the presence of some gene flow. The field survey indicated that remnant traditional trees serve as important ecological corridors facilitating the dispersal of R. gigantea between primary and secondary habitats. However, the expansion of areca and rubber plantations surrounding these remnant trees, along with increased agrochemical use, may further weaken gene flow and population regeneration. This study highlights the genetic status of R. gigantea in fragmented landscapes and provides essential scientific insights for the conservation of its genetic resources and populations within human-influenced environments.
A field survey of Rhynchostylis gigantea was made in fragmented fengshui forest landscapes in Changjiang, Hainan and samples of R. gigantea was collected to analyzed its genetic diversity and population structure by using SNP molecular markers. The results showed that all three local populations (B, M, and J) of R. gigantea maintained moderate levels of genetic diversity (Pi = 0.137–0.147, He = 0.132–0.145), whereas the observed heterozygosity was relatively low (Ho = 0.105–0.122), indicating mild to moderate inbreeding (Fis = 0.049–0.107). The genetic differentiation among the populations was moderate to low (Fst = 0.026–0.058), with the greatest divergence observed between the populations M and J. STRUCTURE, PCA, and UPGMA analyses consistently revealed moderate genetic differentiation among the three populations occurring in human-influenced environments, suggesting the presence of some gene flow. The field survey indicated that remnant traditional trees serve as important ecological corridors facilitating the dispersal of R. gigantea between primary and secondary habitats. However, the expansion of areca and rubber plantations surrounding these remnant trees, along with increased agrochemical use, may further weaken gene flow and population regeneration. This study highlights the genetic status of R. gigantea in fragmented landscapes and provides essential scientific insights for the conservation of its genetic resources and populations within human-influenced environments.
, Available online , doi: 10.15886/j.cnki.rdswxb.20260023
Abstract:
Seeds of four superior soybean varieties carried by the "Shijian-19" satellite were selected for experiment to explore the effects of space mutation on the photosynthetic pigments and microscopic structure of leaves in different soybean varieties. After space mutation treatment, the plants of SP1 generation of these four soybean varieties were planted on the ground. The soybean seeds that were not carried by the satellete were used as the control (CK). The photosynthetic pigments and microscopic structure parameters of the leaves of the soybean plants under space mutation treatment and the control were measured and analyzed. The results showed that the soybean varieties Deshun 34, Deshun 14, and Dongnong 219 under space mutation were 12.91% to 26.38% higher, significantly higher in total chlorophyll content than the control. After space mutation, the carotenoid content of each variety was 28.93% to 51.40% higher, significantly higher than that of the control. After space mutation, the cell morphology, arrangement, and thickness of the leaves of each soybean variety changed significantly. The leaf cells of Deshun 14 were plump, arranged neatly, and had small intercellular spaces. After space mutation, the leaf thickness, spongy tissue thickness, and cell structure compactness of Deshun 14 soybeans were highly significantly higher than those of the control, and the palisade tissue thickness/spongy tissue thickness of Dongnong 219 was highly significantly higher than that of the control, with an increase of 18.52%. However, the cell structure looseness of Deshun 14 and Dongnong 219 was lower than that of the control, decreasing by 10.64% to 18.17%. This experiment provides basic materials for soybean germplasm improvement and variety selection, and offers a foundation and reference for further soybean space mutation breeding and the application of space mutagenesis in the genetic improvement of leguminous crops.
Seeds of four superior soybean varieties carried by the "Shijian-19" satellite were selected for experiment to explore the effects of space mutation on the photosynthetic pigments and microscopic structure of leaves in different soybean varieties. After space mutation treatment, the plants of SP1 generation of these four soybean varieties were planted on the ground. The soybean seeds that were not carried by the satellete were used as the control (CK). The photosynthetic pigments and microscopic structure parameters of the leaves of the soybean plants under space mutation treatment and the control were measured and analyzed. The results showed that the soybean varieties Deshun 34, Deshun 14, and Dongnong 219 under space mutation were 12.91% to 26.38% higher, significantly higher in total chlorophyll content than the control. After space mutation, the carotenoid content of each variety was 28.93% to 51.40% higher, significantly higher than that of the control. After space mutation, the cell morphology, arrangement, and thickness of the leaves of each soybean variety changed significantly. The leaf cells of Deshun 14 were plump, arranged neatly, and had small intercellular spaces. After space mutation, the leaf thickness, spongy tissue thickness, and cell structure compactness of Deshun 14 soybeans were highly significantly higher than those of the control, and the palisade tissue thickness/spongy tissue thickness of Dongnong 219 was highly significantly higher than that of the control, with an increase of 18.52%. However, the cell structure looseness of Deshun 14 and Dongnong 219 was lower than that of the control, decreasing by 10.64% to 18.17%. This experiment provides basic materials for soybean germplasm improvement and variety selection, and offers a foundation and reference for further soybean space mutation breeding and the application of space mutagenesis in the genetic improvement of leguminous crops.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250161
Abstract:
To investigate the effects of space mutagenesis treatment on the growth and physiological characteristics of Senna surattensis and Acacia podalyriifolia, container-grown seedlings were subjected to measurements of growth traits, leaf morphology, photosynthetic parameters, and chlorophyll fluorescence parameters following seed recovery via the new-generation manned spacecraft. Results indicated the treated S. surattensis group exhibited a 12.85% reduction in plant height during the first year, followed by highly significant increases of 100.66% and 66.34% in the second and third years, respectively. Its stem diameter at ground level increased significantly by 11.05% and 43.80% over two years, and its leaf length and width increased by 5.38% and 1.64%, respectively. In the treated A. podalyriifolia group, the plant height decreased by 9.59% and 20.06% in the first two years, then increased significantly by 20.72% in the third year; the stem diameter decreased by 9.71% in the second year and increased slightly by 1.81% in the third year; the leaf width increased by 6.79%, while the leaf length showed no significant change. Regarding to photosynthetic traits, the treated S. surattensis group exhibited a significant 27.58% increase in stomatal conductance (Gs), alongside elevated transpiration rate (Tr), net photosynthetic rate (Pn), and intercellular CO2 concentration (Ci). However, water use efficiency (WUE) decreased by 34.12%. The treated A. podalyriifolia exhibited a 43.11% improvement in WUE, with no significant changes in Pn and Ci, while Tr and Gs decreased by 25.94% and 7.33%, respectively. Regarding to chlorophyll fluorescence parameters, the treated S. surattensis group demonstrated an increase in maximum photochemical efficiency (Fv/Fm), apparent electron transport rate (ETR), non-photochemical quenching (NPQ), photochemical quenching (qP), and actual photochemical efficiency (Y (II))by 1.72%, 32.26%, 7.98%, 28.59%, and 31.92%, respectively, indicating enhanced photosynthetic capacity. In contrast, the ETR and Y (II)of the treated A. podalyriifolia decreased significantly by 24.89% and 24.96%, respectively, while only the NPQ increased by 8.56%, indicating suppressed light energy conversion efficiency. In summary, space mutagenesis treatment exerted significant effects on the growth and physiological characteristics of container-grown seedlings of the two species of woody ornamental plants, though the direction of mutagenesis varied between species.
To investigate the effects of space mutagenesis treatment on the growth and physiological characteristics of Senna surattensis and Acacia podalyriifolia, container-grown seedlings were subjected to measurements of growth traits, leaf morphology, photosynthetic parameters, and chlorophyll fluorescence parameters following seed recovery via the new-generation manned spacecraft. Results indicated the treated S. surattensis group exhibited a 12.85% reduction in plant height during the first year, followed by highly significant increases of 100.66% and 66.34% in the second and third years, respectively. Its stem diameter at ground level increased significantly by 11.05% and 43.80% over two years, and its leaf length and width increased by 5.38% and 1.64%, respectively. In the treated A. podalyriifolia group, the plant height decreased by 9.59% and 20.06% in the first two years, then increased significantly by 20.72% in the third year; the stem diameter decreased by 9.71% in the second year and increased slightly by 1.81% in the third year; the leaf width increased by 6.79%, while the leaf length showed no significant change. Regarding to photosynthetic traits, the treated S. surattensis group exhibited a significant 27.58% increase in stomatal conductance (Gs), alongside elevated transpiration rate (Tr), net photosynthetic rate (Pn), and intercellular CO2 concentration (Ci). However, water use efficiency (WUE) decreased by 34.12%. The treated A. podalyriifolia exhibited a 43.11% improvement in WUE, with no significant changes in Pn and Ci, while Tr and Gs decreased by 25.94% and 7.33%, respectively. Regarding to chlorophyll fluorescence parameters, the treated S. surattensis group demonstrated an increase in maximum photochemical efficiency (Fv/Fm), apparent electron transport rate (ETR), non-photochemical quenching (NPQ), photochemical quenching (qP), and actual photochemical efficiency (Y (II))by 1.72%, 32.26%, 7.98%, 28.59%, and 31.92%, respectively, indicating enhanced photosynthetic capacity. In contrast, the ETR and Y (II)of the treated A. podalyriifolia decreased significantly by 24.89% and 24.96%, respectively, while only the NPQ increased by 8.56%, indicating suppressed light energy conversion efficiency. In summary, space mutagenesis treatment exerted significant effects on the growth and physiological characteristics of container-grown seedlings of the two species of woody ornamental plants, though the direction of mutagenesis varied between species.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250198
Abstract:
The excisionase XisF4, encoded by the filamentous bacteriophage Pf4, is a key protein mediating prophage excision and transcriptional regulation. The molecular characteristics and biological functions of the excisionase XisF4 were systematically elucidated through the integration of bioinformatics, proteomics, and evolutionary analyses. Bioinformatics analysis revealed that XisF4 consists of 71 amino acids and contains a typical MerR-type helix-turn-helix DNA-binding domain. Induced expression and proteomic analysis showed that XisF4 expression led to the differential expression of 94 host proteins, significantly enriched in pathways such as glycine metabolism, biofilm formation, and pyocyanin biosynthesis, while simultaneously inhibiting the expression of proteins associated with the type VI secretion system (e.g., HcpC) and DNA replication. RT-qPCR validated transcriptional changes in key genes, and further EMSA experiments confirmed that purified XisF4 protein directly binds to the promoter regions of host genes phz2 and hcpC, demonstrating its direct transcriptional regulatory capability. Phylogenetic analysis revealed that XisF4 and XisF5 belong to distinct evolutionary branches, and that their evolutionary trajectories differ from that of the bacteriophage capsid protein CoaA, suggesting that independent genetic events may have occurred between the regulatory and structural modules of filamentous bacteriophages during evolution. All the results demonstrate that XisF4 is not only a key enzyme for bacteriophage excision but also functions as a pleiotropic transcriptional regulator, extensively involved in regulating virulence, metabolism, and stress adaptation in the host Pseudomonas aeruginosa. These findings provide an important theoretical foundation for a deeper understanding of bacteriophage-host interaction mechanisms and the development of potential intervention strategies.
The excisionase XisF4, encoded by the filamentous bacteriophage Pf4, is a key protein mediating prophage excision and transcriptional regulation. The molecular characteristics and biological functions of the excisionase XisF4 were systematically elucidated through the integration of bioinformatics, proteomics, and evolutionary analyses. Bioinformatics analysis revealed that XisF4 consists of 71 amino acids and contains a typical MerR-type helix-turn-helix DNA-binding domain. Induced expression and proteomic analysis showed that XisF4 expression led to the differential expression of 94 host proteins, significantly enriched in pathways such as glycine metabolism, biofilm formation, and pyocyanin biosynthesis, while simultaneously inhibiting the expression of proteins associated with the type VI secretion system (e.g., HcpC) and DNA replication. RT-qPCR validated transcriptional changes in key genes, and further EMSA experiments confirmed that purified XisF4 protein directly binds to the promoter regions of host genes phz2 and hcpC, demonstrating its direct transcriptional regulatory capability. Phylogenetic analysis revealed that XisF4 and XisF5 belong to distinct evolutionary branches, and that their evolutionary trajectories differ from that of the bacteriophage capsid protein CoaA, suggesting that independent genetic events may have occurred between the regulatory and structural modules of filamentous bacteriophages during evolution. All the results demonstrate that XisF4 is not only a key enzyme for bacteriophage excision but also functions as a pleiotropic transcriptional regulator, extensively involved in regulating virulence, metabolism, and stress adaptation in the host Pseudomonas aeruginosa. These findings provide an important theoretical foundation for a deeper understanding of bacteriophage-host interaction mechanisms and the development of potential intervention strategies.
, Available online , doi: 10.15886/j.cnki.rdswxb.20260007
Abstract:
An attempt was made to investigate the species of ectoparasitic fleas on the body surface of dogs and the pathogenic microorganisms they carry in Hainan to provide reliable data support for the scientific prevention and control of flea-borne diseases. Ectoparasitic fleas were collected from dogs in three regions of Hainan Province, namely Haikou (HK), Ding'an (DA), and Wenchang (WC), using the reverse hair brushing method combined with fine forceps. Flea species were identified by morphological observation and polymerase chain reaction (PCR). Metagenomic sequencing was used to analyze the microbial community composition and gene functions within the fleas. Results showed that the ectoparasitic flea species identified on the dogs in different regions of Hainan was Ctenocephalides felis. Alpha diversity analysis showed that there were significant overall differences among the three groups of samples (F=49.43, P<0.001). The WC and HK samples were significantly higher in microbial community richness than the DA samples, Among the samples the WC samples was the highest in microbial community diversity, and no significant difference was observed between the HK and WC samples (P>0.05). Beta diversity analysis showed that the three samples had overlapping regions, indicating a high degree of similarity in their microbial community structures. In contrast, the DA samples were clearly separated from the WC samples, showing distinct differences in their microbial communities, and the DA samples formed an independent cluster, indicating that their microbial community structure was significantly different from that of the WC and HK samples (P < 0.05). At the phylum level, Pseudomonadota was the dominant bacterial phylum in all three samples; Bacillota was the second most dominant phylum in the HK and DA samples; Mycoplasmatota was the second most dominant phylum in the WC samples. At the species level, several bacteria posing pathogenic threats to humans and animals were detected, including Rickettsia felis, Klebsiella pneumoniae, Rickettsia tillamookensis, Rickettsia massiliae, Pseudomonas aeruginosa, and Borreliella garinii. Conclusion The findings confirmed Ctenocephalides felis as the dominant ectoparasitic flea species on the dogs in Hainan. The high abundance of pathogenic microorganisms carried by this flea species indicates a relatively high potential risk of flea-borne zoonotic disease outbreaks in this region, providing a scientific basis for targeted surveillance and the formulation of prevention and control strategies for flea-borne diseases in Hainan.
An attempt was made to investigate the species of ectoparasitic fleas on the body surface of dogs and the pathogenic microorganisms they carry in Hainan to provide reliable data support for the scientific prevention and control of flea-borne diseases. Ectoparasitic fleas were collected from dogs in three regions of Hainan Province, namely Haikou (HK), Ding'an (DA), and Wenchang (WC), using the reverse hair brushing method combined with fine forceps. Flea species were identified by morphological observation and polymerase chain reaction (PCR). Metagenomic sequencing was used to analyze the microbial community composition and gene functions within the fleas. Results showed that the ectoparasitic flea species identified on the dogs in different regions of Hainan was Ctenocephalides felis. Alpha diversity analysis showed that there were significant overall differences among the three groups of samples (F=49.43, P<0.001). The WC and HK samples were significantly higher in microbial community richness than the DA samples, Among the samples the WC samples was the highest in microbial community diversity, and no significant difference was observed between the HK and WC samples (P>0.05). Beta diversity analysis showed that the three samples had overlapping regions, indicating a high degree of similarity in their microbial community structures. In contrast, the DA samples were clearly separated from the WC samples, showing distinct differences in their microbial communities, and the DA samples formed an independent cluster, indicating that their microbial community structure was significantly different from that of the WC and HK samples (P < 0.05). At the phylum level, Pseudomonadota was the dominant bacterial phylum in all three samples; Bacillota was the second most dominant phylum in the HK and DA samples; Mycoplasmatota was the second most dominant phylum in the WC samples. At the species level, several bacteria posing pathogenic threats to humans and animals were detected, including Rickettsia felis, Klebsiella pneumoniae, Rickettsia tillamookensis, Rickettsia massiliae, Pseudomonas aeruginosa, and Borreliella garinii. Conclusion The findings confirmed Ctenocephalides felis as the dominant ectoparasitic flea species on the dogs in Hainan. The high abundance of pathogenic microorganisms carried by this flea species indicates a relatively high potential risk of flea-borne zoonotic disease outbreaks in this region, providing a scientific basis for targeted surveillance and the formulation of prevention and control strategies for flea-borne diseases in Hainan.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250155
Abstract:
To clarify the species of ticks on the body surface of animals, characteristics of tick microbial composition, as well as the species and distribution of tick-borne Ehrlichia pathogens in different habitats of Hainan Province, ticks were collected from the body surface of swine and dogs in three administrative regions of Hainan, namely Ding'an County (central Hainan), Baisha Li and Miao Autonomous County (western Hainan), and Wanning City (southern Hainan). Tick species were identified through morphological observation and PCR assay: Haemaphysalis longicornis was collected from dogs in Wanning, while Rhipicephalus sanguineus was isolated from dogs in Ding'an and swine in Baisha. Metagenomic approaches were further employed to analyze the microbial composition, functional pathways and species of tick-borne Ehrlichia in the collected ticks. The results showed that, Proteobacteria was the absolutely dominant bacterial phylum in ticks across the three regions. For fungi, Ascomycota was the dominant phylum in Ding'an ticks, whereas Mucoromycota predominated in ticks from Baisha and Wanning. In terms of viruses, Chrysoviridae was the dominant viral phylum in Ding'an ticks, while Negarnaviricota was the major one in Baisha and Wanning ticks. At the species level, a variety of important zoonotic pathogens were detected, including Coxiella burnetii, Anaplasma phagocytophilum and Ehrlichia spp., with Ehrlichia minasensis being identified in ticks from Hainan for the first time. The functional pathways of the tick microbial communities exhibited obvious intergroup divergence: ticks from Baisha showed high abundances of pathways related to metabolism and drug resistance, while those from Ding'an were characterized by a prominent enrichment of pathways associated with human diseases. Comprehensive analysis revealed that sampling location, tick species and host species exerted significant effects on the tick microbial community structure. Further verification via PCR assays confirmed that the dominant species of Ehrlichia carried by ticks in Hainan were E.canis and E. minasensis. The findings provide a theoretical basis for the prevention and control of tick-borne diseases in Hainan Province, and also offer fundamental data support for the subsequent development of diagnostic methods for tick-borne ehrlichiosis.
To clarify the species of ticks on the body surface of animals, characteristics of tick microbial composition, as well as the species and distribution of tick-borne Ehrlichia pathogens in different habitats of Hainan Province, ticks were collected from the body surface of swine and dogs in three administrative regions of Hainan, namely Ding'an County (central Hainan), Baisha Li and Miao Autonomous County (western Hainan), and Wanning City (southern Hainan). Tick species were identified through morphological observation and PCR assay: Haemaphysalis longicornis was collected from dogs in Wanning, while Rhipicephalus sanguineus was isolated from dogs in Ding'an and swine in Baisha. Metagenomic approaches were further employed to analyze the microbial composition, functional pathways and species of tick-borne Ehrlichia in the collected ticks. The results showed that, Proteobacteria was the absolutely dominant bacterial phylum in ticks across the three regions. For fungi, Ascomycota was the dominant phylum in Ding'an ticks, whereas Mucoromycota predominated in ticks from Baisha and Wanning. In terms of viruses, Chrysoviridae was the dominant viral phylum in Ding'an ticks, while Negarnaviricota was the major one in Baisha and Wanning ticks. At the species level, a variety of important zoonotic pathogens were detected, including Coxiella burnetii, Anaplasma phagocytophilum and Ehrlichia spp., with Ehrlichia minasensis being identified in ticks from Hainan for the first time. The functional pathways of the tick microbial communities exhibited obvious intergroup divergence: ticks from Baisha showed high abundances of pathways related to metabolism and drug resistance, while those from Ding'an were characterized by a prominent enrichment of pathways associated with human diseases. Comprehensive analysis revealed that sampling location, tick species and host species exerted significant effects on the tick microbial community structure. Further verification via PCR assays confirmed that the dominant species of Ehrlichia carried by ticks in Hainan were E.canis and E. minasensis. The findings provide a theoretical basis for the prevention and control of tick-borne diseases in Hainan Province, and also offer fundamental data support for the subsequent development of diagnostic methods for tick-borne ehrlichiosis.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250183
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An attempt was made to analyze the therapeutic effect of combined DNA and protein vaccines on a mouse model of dust mite allergy induced by a fusion allergen. In this attempt eukaryotic and prokaryotic expression plasmids of the major dust mite allergen fusion epitope were constructed to prepare a combined DNA and protein vaccine. A mouse model of dust mite allergy was induced by the fusion allergen Der p 1-Der f 1-Blo t 5. The mice were randomly divided into an allergy group (Allergy) and a vaccine group, with 6 mice in each group. A normal group (Naïve) was set as a control. The vaccine group was injected with the corresponding vaccine, the allergy group was injected with an equal volume of PBS (phosphate buffered saline), and the normal group received no treatment. The therapeutic effect of the vaccine was comprehensively evaluated by detecting the total IgE (immunoglobulin E) level in the mouse serum, changes in airway hyperresponsiveness, the area of dye leakage in the ear, and pathological features of lung sections. The results showed that compared with the allergy group, the combined DNA and protein vaccine group had significantly lower total IgE levels of serum, significantly reduced airway hyper-responsiveness, had significantly smaller area of dye leakage in the mouse ear, alleviated lung tissue inflammatory cell infiltration, reduced average goblet cell numbers in the bronchus, and decreased collagen fiber deposition. It is concluded that the combined DNA and protein vaccine has a good immunotherapeutic effect on the dust mite allergy mouse model, which can provide an important reference for the development of preventive and therapeutic vaccines for dust mite allergy.
An attempt was made to analyze the therapeutic effect of combined DNA and protein vaccines on a mouse model of dust mite allergy induced by a fusion allergen. In this attempt eukaryotic and prokaryotic expression plasmids of the major dust mite allergen fusion epitope were constructed to prepare a combined DNA and protein vaccine. A mouse model of dust mite allergy was induced by the fusion allergen Der p 1-Der f 1-Blo t 5. The mice were randomly divided into an allergy group (Allergy) and a vaccine group, with 6 mice in each group. A normal group (Naïve) was set as a control. The vaccine group was injected with the corresponding vaccine, the allergy group was injected with an equal volume of PBS (phosphate buffered saline), and the normal group received no treatment. The therapeutic effect of the vaccine was comprehensively evaluated by detecting the total IgE (immunoglobulin E) level in the mouse serum, changes in airway hyperresponsiveness, the area of dye leakage in the ear, and pathological features of lung sections. The results showed that compared with the allergy group, the combined DNA and protein vaccine group had significantly lower total IgE levels of serum, significantly reduced airway hyper-responsiveness, had significantly smaller area of dye leakage in the mouse ear, alleviated lung tissue inflammatory cell infiltration, reduced average goblet cell numbers in the bronchus, and decreased collagen fiber deposition. It is concluded that the combined DNA and protein vaccine has a good immunotherapeutic effect on the dust mite allergy mouse model, which can provide an important reference for the development of preventive and therapeutic vaccines for dust mite allergy.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250160
Abstract:
To investigate the infection status, molecular characteristics, and genetic relationship of Ehrlichia canis in Hainan black goats, a total of 1 061 anti-coagulated blood samples were collected from eight cities/counties (Haikou, Chengmai, Dingan, Baisha, Dongfang, Wenchang, Wuzhishan, Danzhou) of Hainan Province. Detection and phylogenetic analysis were performed by PCR amplification targeting the 16S rRNA and groEL genes of E. canis. The results showed there were 34 positive samples, with an overall positive rate of 3.2%. The samples in Dingan County had the highest detection rate (11.40%), suggesting regional prevalence of the pathogen in this area. Phylogenetic analysis indicated that E. canis strains from Hainan black goats were clustered into the same branch, possibly originating from a common infection source. The 16S rRNA sequences of E. canis from Hainan black goats were most closely related to those of a Brazilian strain (KF972447.1), while the groEL gene showed the highest affinity with a Beijing strain from China (MW428317.1), forming a paralogous group with sequences from Beijing and Yunnan in China, reflecting geographic genetic differentiation. These findings provide the first molecular evidence of E. canis in Hainan black goats, suggesting that E. canis may achieve cross-host transmission via ticks, which offers a reference for further research on the molecular epidemiology and control of E. canis.
To investigate the infection status, molecular characteristics, and genetic relationship of Ehrlichia canis in Hainan black goats, a total of 1 061 anti-coagulated blood samples were collected from eight cities/counties (Haikou, Chengmai, Dingan, Baisha, Dongfang, Wenchang, Wuzhishan, Danzhou) of Hainan Province. Detection and phylogenetic analysis were performed by PCR amplification targeting the 16S rRNA and groEL genes of E. canis. The results showed there were 34 positive samples, with an overall positive rate of 3.2%. The samples in Dingan County had the highest detection rate (11.40%), suggesting regional prevalence of the pathogen in this area. Phylogenetic analysis indicated that E. canis strains from Hainan black goats were clustered into the same branch, possibly originating from a common infection source. The 16S rRNA sequences of E. canis from Hainan black goats were most closely related to those of a Brazilian strain (KF972447.1), while the groEL gene showed the highest affinity with a Beijing strain from China (MW428317.1), forming a paralogous group with sequences from Beijing and Yunnan in China, reflecting geographic genetic differentiation. These findings provide the first molecular evidence of E. canis in Hainan black goats, suggesting that E. canis may achieve cross-host transmission via ticks, which offers a reference for further research on the molecular epidemiology and control of E. canis.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250148
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An analysis was made of the molecular characteristics of type III polyketide synthases (PKSIII) in Alpinia oxyphylla and their potential role in the biosynthesis of diphenylheptanoid compounds. The PKSIII gene family was identified from the A. oxyphylla genome, and the candidate gene AoPKS5 was selected for structural and expression analysis. Based on genomic and transcriptomic data, PKSIII genes were systematically screened and analyzed for expression levels, and phylogenetic analysis was conducted to explore their evolutionary relationships. Bioinformatics tools were used to predict the physicochemical properties and catalytic sites of AoPKS5. Tissue-specific expression was analyzed by using RT-qPCR, and the recombinant vector pNC-ET28-AoPKS5 was constructed for heterologous expression in Escherichia coli BL21 (DE3). The results showed that AoPKS5 contained a1170 bp ORF encoding 389 amino acids, with a theoretical molecular weight of 42 kDa and an isoelectric point of 5.96, lacking both signal peptide and transmembrane domains, but possessing the conserved Cys–His–Asn catalytic triad and GFGPG motif. Phylogenetic analysis indicated high homology with DCS proteins from Curcuma longa and Zingiber officinale. RT-qPCR revealed that AoPKS5 was most highly expressed in leaves. The AoPKS5 gene was successfully cloned and characterized and its prokaryotic expression system established, providing a theoretical and technical foundation for elucidating the molecular mechanisms of PKSIII involvement in diphenylheptanoid biosynthesis in A. oxyphylla.
An analysis was made of the molecular characteristics of type III polyketide synthases (PKSIII) in Alpinia oxyphylla and their potential role in the biosynthesis of diphenylheptanoid compounds. The PKSIII gene family was identified from the A. oxyphylla genome, and the candidate gene AoPKS5 was selected for structural and expression analysis. Based on genomic and transcriptomic data, PKSIII genes were systematically screened and analyzed for expression levels, and phylogenetic analysis was conducted to explore their evolutionary relationships. Bioinformatics tools were used to predict the physicochemical properties and catalytic sites of AoPKS5. Tissue-specific expression was analyzed by using RT-qPCR, and the recombinant vector pNC-ET28-AoPKS5 was constructed for heterologous expression in Escherichia coli BL21 (DE3). The results showed that AoPKS5 contained a
, Available online , doi: 10.15886/j.cnki.rdswxb.20260010
Abstract:
Hevea brasiliensis is the main source of natural rubber and serves as an important economic crop supporting the tropical agricultural economy in our country. Anthracnose caused by Colletotrichum gloeosporioides is an important reason for reduced natural rubber yield. Very long-chain fatty acids (VLCFAs) play an important role in maintaining cell membrane stability and in the development of pathogen appressorium structures. The very-long-chain fatty acid elongase (Elo) is a rate-limiting enzyme involved in regulating VLCFA synthesis. However, the function of Elo in C. gloeosporioides remains unclear. This study focuses on the very-long-chain fatty acid elongase CgElo3 of C. gloeosporioides. Analysis of physiological phenotype in ΔCgElo3 shows that deletion of CgElo3 does not affect hyphal growth or the formation of appressorium, but it significantly reduces the conidiation of C. gloeosporioides, its pathogenicity on rubber tree leaves, and the formation rate of invasive hyphae during the early infection of onion epidermis. Lipidomic data confirmed that the relative contents of stearic acid, linoleic acid, α-linolenic acid, and eicosatrienoic acid were significantly reduced in ΔCgElo3. The above results indicate that CgElo3 affects the production of conidia and early host cell invasion of C. gloeosporioides by regulating the biosynthesis of VLCFAs, thereby reducing its pathogenicity.
Hevea brasiliensis is the main source of natural rubber and serves as an important economic crop supporting the tropical agricultural economy in our country. Anthracnose caused by Colletotrichum gloeosporioides is an important reason for reduced natural rubber yield. Very long-chain fatty acids (VLCFAs) play an important role in maintaining cell membrane stability and in the development of pathogen appressorium structures. The very-long-chain fatty acid elongase (Elo) is a rate-limiting enzyme involved in regulating VLCFA synthesis. However, the function of Elo in C. gloeosporioides remains unclear. This study focuses on the very-long-chain fatty acid elongase CgElo3 of C. gloeosporioides. Analysis of physiological phenotype in ΔCgElo3 shows that deletion of CgElo3 does not affect hyphal growth or the formation of appressorium, but it significantly reduces the conidiation of C. gloeosporioides, its pathogenicity on rubber tree leaves, and the formation rate of invasive hyphae during the early infection of onion epidermis. Lipidomic data confirmed that the relative contents of stearic acid, linoleic acid, α-linolenic acid, and eicosatrienoic acid were significantly reduced in ΔCgElo3. The above results indicate that CgElo3 affects the production of conidia and early host cell invasion of C. gloeosporioides by regulating the biosynthesis of VLCFAs, thereby reducing its pathogenicity.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250196
Abstract:
Lectin receptor-like kinases (LecRLKs) are a class of key immune-related receptors that play an important role in plant recognition of pathogen signals and the initiation of defense responses. The powdery mildew of rubber tree, Oidium heveae, triggers disease resistance in Arabidopsis. However, it remains unknown whether the LecRLKs are involved in Arabidopsis resistance against O. heveae. In this study, RNA-seq analysis revealed that the lectin receptor gene LecRK-I.7 was up-regulated by 12 hours after O. heveae infection, suggesting that LecRK-I.7 may play a role in resistance to O. heveae. The T-DNA homozygous insertion mutants of LecRK-I.7 were characterized and inoculated with O. heveae. The results showed that compared with wild-type Col-0, O. heveae formed dense hyphal networks and a few conidia in the lecrk-1.7 mutants, and that the cell death and reactive oxygen burst induced by O. heveae in lecrk-1.7 mutants were also greatly reduced than those in WT Col-0. Furthermore, the resistance phenotype of mutant lecrk-1.7 was restored by overexpression of LecRK-I.7 in the mutant, indicating that LecRK-I.7 positively regulates Arabidopsis resistance to O. heveae. It was also confirmed that LecRK-I.7 was mainly localized to the plant cell membrane to fulfill its function, and that the expression of LecRK-I.7 gene could be induced by O. heveae, which provides a basis for further study on the disease resistance mechanisms of Arabidopsis against O. heveae.
Lectin receptor-like kinases (LecRLKs) are a class of key immune-related receptors that play an important role in plant recognition of pathogen signals and the initiation of defense responses. The powdery mildew of rubber tree, Oidium heveae, triggers disease resistance in Arabidopsis. However, it remains unknown whether the LecRLKs are involved in Arabidopsis resistance against O. heveae. In this study, RNA-seq analysis revealed that the lectin receptor gene LecRK-I.7 was up-regulated by 12 hours after O. heveae infection, suggesting that LecRK-I.7 may play a role in resistance to O. heveae. The T-DNA homozygous insertion mutants of LecRK-I.7 were characterized and inoculated with O. heveae. The results showed that compared with wild-type Col-0, O. heveae formed dense hyphal networks and a few conidia in the lecrk-1.7 mutants, and that the cell death and reactive oxygen burst induced by O. heveae in lecrk-1.7 mutants were also greatly reduced than those in WT Col-0. Furthermore, the resistance phenotype of mutant lecrk-1.7 was restored by overexpression of LecRK-I.7 in the mutant, indicating that LecRK-I.7 positively regulates Arabidopsis resistance to O. heveae. It was also confirmed that LecRK-I.7 was mainly localized to the plant cell membrane to fulfill its function, and that the expression of LecRK-I.7 gene could be induced by O. heveae, which provides a basis for further study on the disease resistance mechanisms of Arabidopsis against O. heveae.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250131
Abstract:
To clarify the species composition of major pests in pepper fields in Sanya, Hainan, and the relationship between thrips population dynamics and meteorological factors, a systematic survey of pest species and thrips population dynamics was conducted during the pepper growing season from November 2024 to May 2025, and their association with key meteorological factors was analyzed. The results showed that the dominant pests in Sanya pepper fields were thrips, with Thrips palmi and Frankliniella intonsa being the dominant species. Thrips pests occurred at all the growth stages of pepper, but their population dynamics exhibited distinct growth stage-specific differences: the seedling stage was a low-incidence period, and the flowering and fruit-setting stages were the peak population period, during which the number of F. intonsa significantly exceeded that of T. palmi. The damage decreased during the flowering and harvesting stages, and by the end of the survey, T. palmi once again became dominant. Correlation analysis indicated that meteorological factors during the flowering and fruit-setting stages had a significant influence on thrips population numbers, showing significant positive correlations with average temperature, minimum temperature, average surface temperature, daily maximum/minimum surface temperature, and maximum humidity. All these findings reveal the pest species composition, dominant pest populations, dynamics of dominant thrips pests, and the key influence of meteorological factors on thrips population dynamics in pepper fields, providing a scientific basis for the precise monitoring and formulation of control strategies against thrips pests in pepper fields in the Sanya region.
To clarify the species composition of major pests in pepper fields in Sanya, Hainan, and the relationship between thrips population dynamics and meteorological factors, a systematic survey of pest species and thrips population dynamics was conducted during the pepper growing season from November 2024 to May 2025, and their association with key meteorological factors was analyzed. The results showed that the dominant pests in Sanya pepper fields were thrips, with Thrips palmi and Frankliniella intonsa being the dominant species. Thrips pests occurred at all the growth stages of pepper, but their population dynamics exhibited distinct growth stage-specific differences: the seedling stage was a low-incidence period, and the flowering and fruit-setting stages were the peak population period, during which the number of F. intonsa significantly exceeded that of T. palmi. The damage decreased during the flowering and harvesting stages, and by the end of the survey, T. palmi once again became dominant. Correlation analysis indicated that meteorological factors during the flowering and fruit-setting stages had a significant influence on thrips population numbers, showing significant positive correlations with average temperature, minimum temperature, average surface temperature, daily maximum/minimum surface temperature, and maximum humidity. All these findings reveal the pest species composition, dominant pest populations, dynamics of dominant thrips pests, and the key influence of meteorological factors on thrips population dynamics in pepper fields, providing a scientific basis for the precise monitoring and formulation of control strategies against thrips pests in pepper fields in the Sanya region.
, Available online , doi: 10.15886/j.cnki.rdswxb.20260019
Abstract:
Waltherione A is a plant-derived nematicidal compound with significant activity against root-knot nematodes (LC50 0.09–3.54 mg·L−1). Caenorhabditis elegans was used as a model organism to elucidate the action mechanism of Waltherinone A. Based on a pre-determined screening concentration of 10 mg·L−1, phenotypic differences between wild-type N2 and the Waltherione A-resistant mutant A1 were compared at treatment concentrations of 3 and 6 mg·L−1, enabling a basic phenotypic characterization of the mutant. Systematic screening via SNP mapping and whole-genome resequencing, combined with RNAi validation, identified three potential target genes: Y32H12A.4 (szy-2), F47D12.4 (hmg-1.2), and C28A5.4 (ceh-43). Molecular docking simulations indicated that Waltherione A can form stable, high-affinity complexes with the corresponding proteins (binding energies of −6.3, −7.1, and −6.9 kcal·mol−1, respectively). The results suggest that Waltherione A may exert its nematicidal effect by simultaneously targeting the transcription regulator HMG-1.2, the developmental programming factor CEH-43, and the phosphatase signaling protein SZY-2, thereby disrupting a coordinated response pathway governed by transcriptional and developmental regulation with phosphatase signaling as a key execution node. All these findings systematically reveal the multi-target network of Waltherione A, providing an important theoretical basis for its further development and mechanistic application as a novel green nematicide.
Waltherione A is a plant-derived nematicidal compound with significant activity against root-knot nematodes (LC50 0.09–3.54 mg·L−1). Caenorhabditis elegans was used as a model organism to elucidate the action mechanism of Waltherinone A. Based on a pre-determined screening concentration of 10 mg·L−1, phenotypic differences between wild-type N2 and the Waltherione A-resistant mutant A1 were compared at treatment concentrations of 3 and 6 mg·L−1, enabling a basic phenotypic characterization of the mutant. Systematic screening via SNP mapping and whole-genome resequencing, combined with RNAi validation, identified three potential target genes: Y32H12A.4 (szy-2), F47D12.4 (hmg-1.2), and C28A5.4 (ceh-43). Molecular docking simulations indicated that Waltherione A can form stable, high-affinity complexes with the corresponding proteins (binding energies of −6.3, −7.1, and −6.9 kcal·mol−1, respectively). The results suggest that Waltherione A may exert its nematicidal effect by simultaneously targeting the transcription regulator HMG-1.2, the developmental programming factor CEH-43, and the phosphatase signaling protein SZY-2, thereby disrupting a coordinated response pathway governed by transcriptional and developmental regulation with phosphatase signaling as a key execution node. All these findings systematically reveal the multi-target network of Waltherione A, providing an important theoretical basis for its further development and mechanistic application as a novel green nematicide.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250205
Abstract:
With arable land resources becoming increasingly scarce, extreme weather events occurring more frequently, and population growth accelerating, plant factories have gradually emerged as a vital form of agricultural production due to their advantages of minimal land requirements and controllable production environments. However, there remains a lack of wheat varieties specifically suited for cultivation and production in plant factories. Therefore, an attempt was made to develop new wheat germplasm suitable for plant factory production. Through marker-assisted selection, new accessions of dwarf, early-maturing wheat germplasm suitable for plant factory production were successfully developed. Furthermore, through exome sequencing, a novel genetic locus controlling wheat early maturity was identifiedon chromosome 1B. The development of these dwarf, early-maturing wheat germplasm and the identification of a novel genetic locus for early maturity provide a robust material and theoretical foundation for the industrialized production of wheat.
With arable land resources becoming increasingly scarce, extreme weather events occurring more frequently, and population growth accelerating, plant factories have gradually emerged as a vital form of agricultural production due to their advantages of minimal land requirements and controllable production environments. However, there remains a lack of wheat varieties specifically suited for cultivation and production in plant factories. Therefore, an attempt was made to develop new wheat germplasm suitable for plant factory production. Through marker-assisted selection, new accessions of dwarf, early-maturing wheat germplasm suitable for plant factory production were successfully developed. Furthermore, through exome sequencing, a novel genetic locus controlling wheat early maturity was identifiedon chromosome 1B. The development of these dwarf, early-maturing wheat germplasm and the identification of a novel genetic locus for early maturity provide a robust material and theoretical foundation for the industrialized production of wheat.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250178
Abstract:
The subgenus Dasyhelea are the most diverse group of the genus Dasyhelea (Diptera: Ceratopogonidae: Dasyhelea) including over 400 species worldwide, with 56 species from China and only four from Hainan Island. Based on the systematic collections in the tropical rainforests of Hainan Island and their morphological and DNA barcoding (COⅠ) analyses, two new species of Dasyhelea, Dasyhelea limushanensis sp. nov. and D. bifurca sp. nov., were described, and a newly recorded from Hainan Island, D. abronica Yu, 2001, were also reported. The morphological characteristics of the new species were described in detail, and their identification diagrams and the cytochrome c oxidase subunit I (COI) barcoding data were presented.
The subgenus Dasyhelea are the most diverse group of the genus Dasyhelea (Diptera: Ceratopogonidae: Dasyhelea) including over 400 species worldwide, with 56 species from China and only four from Hainan Island. Based on the systematic collections in the tropical rainforests of Hainan Island and their morphological and DNA barcoding (COⅠ) analyses, two new species of Dasyhelea, Dasyhelea limushanensis sp. nov. and D. bifurca sp. nov., were described, and a newly recorded from Hainan Island, D. abronica Yu, 2001, were also reported. The morphological characteristics of the new species were described in detail, and their identification diagrams and the cytochrome c oxidase subunit I (COI) barcoding data were presented.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250189
Abstract:
Cryptolaemus montrouzieri (Mulsant) is one of the most important predatory natural enemies for controlling Planococcus minor (Maskell). To screen chemical insecticides with high efficacy against P. minor while maintaining safety for C. montrouzieri, six insecticides were selected for evaluation of their toxicity against adult female P. minor and fourth-instar larvae and adults of C. montrouzieri using dipping and filter paper contact methods. The safety to the natural enemy was assessed by using beneficial-harmful toxicity ratios and safety coefficients. The results showed that after 24-h treatment, the toxicity of six insecticides against P. minor females ranked from the highest to the lowest: acetamiprid 10% EC (LC50 = 3.918 mg·L−1) > sulfoxaflor 22% SC (LC50 =13.343 mg·L−1) > thiamethoxam 25% WG (LC50 =23.290 mg·L−1) > abamectin 1.8% EC (LC50 = 58.173 mg·L−1) > dinotefuran 20% SC (LC50 = 111.008 mg·L−1) > bifenthrin 25 g·L−1 EC (LC50 =141.131 mg·L−1). Sulfoxaflor 22% SC exhibited the lowest toxicity to both fourth-instar larvae and adults of C. montrouzieri, while abamectin 1.8% EC showed the highest toxicity to fourth-instar larvae and bifenthrin 25 g·L−1 EC demonstrated the highest toxicity to adult lady beetles. Based on beneficial-harmful toxicity ratios and safety coefficients, both acetamiprid 10% EC and sulfoxaflor 22% SC exhibited high toxicity against P. minor and low toxicity to C. montrouzieri. It is recommended that these two insecticides be used in rotation for mealybug control in the field to achieve synergistic pest management with high efficacy and natural enemy conservation.
Cryptolaemus montrouzieri (Mulsant) is one of the most important predatory natural enemies for controlling Planococcus minor (Maskell). To screen chemical insecticides with high efficacy against P. minor while maintaining safety for C. montrouzieri, six insecticides were selected for evaluation of their toxicity against adult female P. minor and fourth-instar larvae and adults of C. montrouzieri using dipping and filter paper contact methods. The safety to the natural enemy was assessed by using beneficial-harmful toxicity ratios and safety coefficients. The results showed that after 24-h treatment, the toxicity of six insecticides against P. minor females ranked from the highest to the lowest: acetamiprid 10% EC (LC50 = 3.918 mg·L−1) > sulfoxaflor 22% SC (LC50 =13.343 mg·L−1) > thiamethoxam 25% WG (LC50 =23.290 mg·L−1) > abamectin 1.8% EC (LC50 = 58.173 mg·L−1) > dinotefuran 20% SC (LC50 = 111.008 mg·L−1) > bifenthrin 25 g·L−1 EC (LC50 =141.131 mg·L−1). Sulfoxaflor 22% SC exhibited the lowest toxicity to both fourth-instar larvae and adults of C. montrouzieri, while abamectin 1.8% EC showed the highest toxicity to fourth-instar larvae and bifenthrin 25 g·L−1 EC demonstrated the highest toxicity to adult lady beetles. Based on beneficial-harmful toxicity ratios and safety coefficients, both acetamiprid 10% EC and sulfoxaflor 22% SC exhibited high toxicity against P. minor and low toxicity to C. montrouzieri. It is recommended that these two insecticides be used in rotation for mealybug control in the field to achieve synergistic pest management with high efficacy and natural enemy conservation.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250144
Abstract:
An attempt was made to improve the utilization efficiency of the precious land resources for seed production in Hainan and to ensure the production of high-quality soybean seeds. The effects of planting density on the yield, photosynthetic physiology, agronomic traits, and quality characteristics of different soybean genotypes under Hainan's ecological conditions were analyzed to provide a theoretical basis for rational close planting. Ten dominant soybean varieties recommended by the General Office of the Ministry of Agriculture and Rural Affairs of China were selected, and planted at four planting densities, 400,000, 600,000, 800,000, and 1,000,000 plants per hectare (or D1 to D4, respectively). A systematic analysis was conducted on the effects of density on soybean agronomic traits, quality parameters, photosynthetic characteristics, and yield components. The results indicated that with an increase in planting density, the flowering and maturity stages of the soybeans showed no significant changes. The number of grains per plant, pods per plant, and effective nodes all decreased significantly. Plant height exhibited a trend of either gradual decline or an initial increase followed by a decrease. Density treatments significantly regulated quality traits and photosynthetic parameters: protein content generally showed a decreasing trend or an initial increase followed by a decrease, and fat content commonly declined while the net photosynthetic rate (Pn) demonstrated an increasing trend with density. The yield response to density varied significantly among varieties. Most varieties, such as Suinong 52 and Heinong 531, achieved their highest yield at low to medium densities (D1 or D2), whereas a few varieties, such as Zhonghuang 901 and Heinong 84, maintained high yield even at relatively high densities (D3). All these findings clarify the complex physiological mechanisms through which planting density regulates soybean yield and quality formation by influencing light use efficiency and plant architecture performance. The results highlight the necessity of determining suitable planting densities based on varietal characteristics, providing a scientific basis and theoretical reference for achieving high-quality and high-yield soybean cultivation in the Hainan region.
An attempt was made to improve the utilization efficiency of the precious land resources for seed production in Hainan and to ensure the production of high-quality soybean seeds. The effects of planting density on the yield, photosynthetic physiology, agronomic traits, and quality characteristics of different soybean genotypes under Hainan's ecological conditions were analyzed to provide a theoretical basis for rational close planting. Ten dominant soybean varieties recommended by the General Office of the Ministry of Agriculture and Rural Affairs of China were selected, and planted at four planting densities, 400,000, 600,000, 800,000, and 1,000,000 plants per hectare (or D1 to D4, respectively). A systematic analysis was conducted on the effects of density on soybean agronomic traits, quality parameters, photosynthetic characteristics, and yield components. The results indicated that with an increase in planting density, the flowering and maturity stages of the soybeans showed no significant changes. The number of grains per plant, pods per plant, and effective nodes all decreased significantly. Plant height exhibited a trend of either gradual decline or an initial increase followed by a decrease. Density treatments significantly regulated quality traits and photosynthetic parameters: protein content generally showed a decreasing trend or an initial increase followed by a decrease, and fat content commonly declined while the net photosynthetic rate (Pn) demonstrated an increasing trend with density. The yield response to density varied significantly among varieties. Most varieties, such as Suinong 52 and Heinong 531, achieved their highest yield at low to medium densities (D1 or D2), whereas a few varieties, such as Zhonghuang 901 and Heinong 84, maintained high yield even at relatively high densities (D3). All these findings clarify the complex physiological mechanisms through which planting density regulates soybean yield and quality formation by influencing light use efficiency and plant architecture performance. The results highlight the necessity of determining suitable planting densities based on varietal characteristics, providing a scientific basis and theoretical reference for achieving high-quality and high-yield soybean cultivation in the Hainan region.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250188
Abstract:
Natural rubber is a critical strategic resource in China, and anthracnose of rubber trees caused by Colletotrichum gloeosporioides directly threatens the healthy development of the rubber industry. During infection, C. gloeosporioides secretes numerous protein effectors, and their roles in overcoming plant immunity remain poorly understood. Investigating potential effector proteins in C. gloeosporioides can provide a theoretical basis for controlling anthracnose. In this context, two candidate effector proteins, Cg694 and Cg2346, were predicted in C. gloeosporioides. Quantitative real-time PCR revealed that the transcriptional expression of the genes Cg694 and Cg2346 was significantly upregulated during the development stages of spore, germination, and appressorium compared with the vegetative hyphal stage. Subcellular localization assays via transient expression (Green fluorescent protein) in Nicotiana benthamiana epidermal cells showed that both Cg694 and Cg2346 genes were localized to the nucleus and cell membrane, and their transient overexpression induced necrosis in tobacco leaves. Furthermore, gene knockout mutants ΔCg694 and ΔCg2346 were generated. Phenotypic analysis indicated that ΔCg694 and ΔCg2346 exhibit enhanced germ tube polar growth and reduced early invasive hyphal formation rates in onion epidermal cells. Notably, the ΔCg2346 mutant also show a decreased ability to form appressoria. However, neither ΔCg694 nor ΔCg2346 mutants display significant changes in pathogenicity on rubber tree leaves. These results demonstrate that Cg694 and Cg2346 are involved in modulating the polar growth and early invasion capabilities of C. gloeosporioides, though their precise functions require further investigation.
Natural rubber is a critical strategic resource in China, and anthracnose of rubber trees caused by Colletotrichum gloeosporioides directly threatens the healthy development of the rubber industry. During infection, C. gloeosporioides secretes numerous protein effectors, and their roles in overcoming plant immunity remain poorly understood. Investigating potential effector proteins in C. gloeosporioides can provide a theoretical basis for controlling anthracnose. In this context, two candidate effector proteins, Cg694 and Cg2346, were predicted in C. gloeosporioides. Quantitative real-time PCR revealed that the transcriptional expression of the genes Cg694 and Cg2346 was significantly upregulated during the development stages of spore, germination, and appressorium compared with the vegetative hyphal stage. Subcellular localization assays via transient expression (Green fluorescent protein) in Nicotiana benthamiana epidermal cells showed that both Cg694 and Cg2346 genes were localized to the nucleus and cell membrane, and their transient overexpression induced necrosis in tobacco leaves. Furthermore, gene knockout mutants ΔCg694 and ΔCg2346 were generated. Phenotypic analysis indicated that ΔCg694 and ΔCg2346 exhibit enhanced germ tube polar growth and reduced early invasive hyphal formation rates in onion epidermal cells. Notably, the ΔCg2346 mutant also show a decreased ability to form appressoria. However, neither ΔCg694 nor ΔCg2346 mutants display significant changes in pathogenicity on rubber tree leaves. These results demonstrate that Cg694 and Cg2346 are involved in modulating the polar growth and early invasion capabilities of C. gloeosporioides, though their precise functions require further investigation.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250163
Abstract:
As an important tropical fruit in China, mango holds a critical position in Hainan's agricultural economy. In recent years, the threat posed by bacterial diseases to local mango production has been increasingly severe. Field surveys were conducted from April to May 2025 in the major-producing areas of Hainan Province, including Changjiang, Dongfang, Ledong, and Sanya, and symptomatic samples were collected to perform pathogen isolation and identification. The surveys showed that, in addition to the known bacterial black spot disease, bacterial necrosis was also present. Morphological observation, physiological and biochemical characterization, multilocus phylogenetic analysis based on 16S rRNA, fusA, leuS, pyrG, rplB, and rpoB gene sequences, and pathogenicity tests following Koch’s postulates showed five out of ten isolated strains from the symptomatic samples were identified as Pantoea dispersa, confirming their role as the causal agent of mango bacterial necrosis disease. This disease is reported for the first time to infect mango in Hainan, which provides an important basis for the prevention and control of the disease in the local mango industry.
As an important tropical fruit in China, mango holds a critical position in Hainan's agricultural economy. In recent years, the threat posed by bacterial diseases to local mango production has been increasingly severe. Field surveys were conducted from April to May 2025 in the major-producing areas of Hainan Province, including Changjiang, Dongfang, Ledong, and Sanya, and symptomatic samples were collected to perform pathogen isolation and identification. The surveys showed that, in addition to the known bacterial black spot disease, bacterial necrosis was also present. Morphological observation, physiological and biochemical characterization, multilocus phylogenetic analysis based on 16S rRNA, fusA, leuS, pyrG, rplB, and rpoB gene sequences, and pathogenicity tests following Koch’s postulates showed five out of ten isolated strains from the symptomatic samples were identified as Pantoea dispersa, confirming their role as the causal agent of mango bacterial necrosis disease. This disease is reported for the first time to infect mango in Hainan, which provides an important basis for the prevention and control of the disease in the local mango industry.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250174
Abstract:
Cucumber fusarium wilt, caused by Fusarium oxysporum f.sp. cucumerinum, is one of the most devastating soil-borne fungal diseases, and its effective control remains a significant challenge in agricultural production. A Trichoderma strain HL088 exhibiting pronounced antagonistic activity against the F. oxysporum was obtained through dual culture technique and screening of antifungal metabolites. Morphological and molecular identification showed the strain HL088 is Trichoderma paratroviride. Furthermore, a potential antifungal protein, chitosanase TriCho75 was identified from the solid-state fermentation extract of this strain by employing ammonium sulfate precipitation and mass spectrometry analysis. Prokaryotic expression and functional validation demonstrated that the recombinant protein TriCho75 (15 μmol·L−1) effectively inhibited both conidial germination (with an inhibition rate of 96.44%) and mycelial growth (with an inhibition rate of 60.59%) of F. oxysporum. Microscopic examination revealed that the recombinant protein TriCho75 caused shrinkage and malformation of the conidia, leading to hyphal distortion and irregular thickness in F. oxysporum. These results indicate that T. paratroviride HL088 displays its potential as a biocontrol agent for managing plant Fusarium wilt diseases.
Cucumber fusarium wilt, caused by Fusarium oxysporum f.sp. cucumerinum, is one of the most devastating soil-borne fungal diseases, and its effective control remains a significant challenge in agricultural production. A Trichoderma strain HL088 exhibiting pronounced antagonistic activity against the F. oxysporum was obtained through dual culture technique and screening of antifungal metabolites. Morphological and molecular identification showed the strain HL088 is Trichoderma paratroviride. Furthermore, a potential antifungal protein, chitosanase TriCho75 was identified from the solid-state fermentation extract of this strain by employing ammonium sulfate precipitation and mass spectrometry analysis. Prokaryotic expression and functional validation demonstrated that the recombinant protein TriCho75 (15 μmol·L−1) effectively inhibited both conidial germination (with an inhibition rate of 96.44%) and mycelial growth (with an inhibition rate of 60.59%) of F. oxysporum. Microscopic examination revealed that the recombinant protein TriCho75 caused shrinkage and malformation of the conidia, leading to hyphal distortion and irregular thickness in F. oxysporum. These results indicate that T. paratroviride HL088 displays its potential as a biocontrol agent for managing plant Fusarium wilt diseases.
, Available online , doi: 10.15886/j.cnki.rdswxb.20260039
Abstract:
Interspecific and intraspecific hybridization is widespread among Camellia species, and interspecific hybridization and polyploidization are important driving forces for the evolution of this genus. Hybrid breeding is also a key approach for the selection and breeding of superior varieties of Camellia hainanica. However, during the period from August to October each year, frequent strong typhoons cause severe fruit drop in hybridized C. hainanica, which greatly hinders the progress of hybrid breeding. In this study, eight groups of fallen hybrid fruits collected after strong typhoons were used as materials. The fallen hybrid fruits resulted from C. vietnamensis (paternal parent) and different superior varieties of C. hainanica (maternal parents). The immature embryos were peeled off from the fallen fruits and cultured in vitro to observe the embryoerescue effect. The results showed that only some of the embryos from the fallen hybrid fruits could germinate into seedlings on the embryo germination medium (GM), but the cotyledons of hybrid embryos in all the eight groups could be induced to form meristematic nodules on the initial induction medium (IIM) after 10 to 30 days of culture. On the proliferation medium (PM), the proliferation coefficient of the meristematic nodules was 3.1 to 5.0. On the regeneration medium (RM), the early regeneration of the meristematic nodules mainly occurred through direct nodule development and somatic embryo formation, and there were significant differences in the differentiation ability among different hybrid embryos, with the group IV showing the best performance. As the redifferentiation time increased, the meristematic nodules gradually shifted to the formation of adventitious buds mainly through organogenesis, with a proliferation coefficient of 2.7 to 3.5. For subculture proliferation, it is advisable to inoculate 2 to 3 clusters of buds with a length of less than 2 cm. When the bud length is greater than 2 cm, cutting stem segments containing axillary buds or terminal buds for proliferation is prone to enzyme-induced browning, which affects the proliferation effect. Therefore, it is necessary to screen buds with strong anti-browning ability for proliferation, or to control the subculture time within 20 days.
Interspecific and intraspecific hybridization is widespread among Camellia species, and interspecific hybridization and polyploidization are important driving forces for the evolution of this genus. Hybrid breeding is also a key approach for the selection and breeding of superior varieties of Camellia hainanica. However, during the period from August to October each year, frequent strong typhoons cause severe fruit drop in hybridized C. hainanica, which greatly hinders the progress of hybrid breeding. In this study, eight groups of fallen hybrid fruits collected after strong typhoons were used as materials. The fallen hybrid fruits resulted from C. vietnamensis (paternal parent) and different superior varieties of C. hainanica (maternal parents). The immature embryos were peeled off from the fallen fruits and cultured in vitro to observe the embryoerescue effect. The results showed that only some of the embryos from the fallen hybrid fruits could germinate into seedlings on the embryo germination medium (GM), but the cotyledons of hybrid embryos in all the eight groups could be induced to form meristematic nodules on the initial induction medium (IIM) after 10 to 30 days of culture. On the proliferation medium (PM), the proliferation coefficient of the meristematic nodules was 3.1 to 5.0. On the regeneration medium (RM), the early regeneration of the meristematic nodules mainly occurred through direct nodule development and somatic embryo formation, and there were significant differences in the differentiation ability among different hybrid embryos, with the group IV showing the best performance. As the redifferentiation time increased, the meristematic nodules gradually shifted to the formation of adventitious buds mainly through organogenesis, with a proliferation coefficient of 2.7 to 3.5. For subculture proliferation, it is advisable to inoculate 2 to 3 clusters of buds with a length of less than 2 cm. When the bud length is greater than 2 cm, cutting stem segments containing axillary buds or terminal buds for proliferation is prone to enzyme-induced browning, which affects the proliferation effect. Therefore, it is necessary to screen buds with strong anti-browning ability for proliferation, or to control the subculture time within 20 days.
, Available online , doi: 10.15886/j.cnki.rdswxb.20260028
Abstract:
To establish an accurate identification and evaluation system for bitter melon peel color based on image analysis and deep learning, peel color was used as a typical continuous phenotypic trait to develop an integrated system combining color standardization and sequential deep learning. A traditional machine learning model was constructed using Lab color mean features, yielding a baseline classification accuracy of 73.68%. By optimizing the classification criteria through K-means clustering and CIE1976 ΔE color difference quantification, the accuracy was improved to 76.09%. Furthermore, a color sequence dataset was established, and convolutional neural network (CNN) and long short-term memory (LSTM) models were compared. The results demonstrated that the LSTM model effectively captured the dependencies in color sequences, achieving a maximum classification accuracy of 82.11% and a macro-average AUC of 0.9645. These results indicate that the combination of color standardization and sequential deep learning can effectively address the classification challenges caused by continuous color phenotypes. The proposed method provides a reliable theoretical basis and technical reference for high-throughput and accurate identification of peel color in crop germplasm resources.
To establish an accurate identification and evaluation system for bitter melon peel color based on image analysis and deep learning, peel color was used as a typical continuous phenotypic trait to develop an integrated system combining color standardization and sequential deep learning. A traditional machine learning model was constructed using Lab color mean features, yielding a baseline classification accuracy of 73.68%. By optimizing the classification criteria through K-means clustering and CIE1976 ΔE color difference quantification, the accuracy was improved to 76.09%. Furthermore, a color sequence dataset was established, and convolutional neural network (CNN) and long short-term memory (LSTM) models were compared. The results demonstrated that the LSTM model effectively captured the dependencies in color sequences, achieving a maximum classification accuracy of 82.11% and a macro-average AUC of 0.9645. These results indicate that the combination of color standardization and sequential deep learning can effectively address the classification challenges caused by continuous color phenotypes. The proposed method provides a reliable theoretical basis and technical reference for high-throughput and accurate identification of peel color in crop germplasm resources.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250207
Abstract:
A method based on UPLC-Q-TOF-MS was established for the systematic characterization and comparison of metabolic profiles and in vitro antioxidant activities in the aerial parts of Houttuynia cordata under different harvest methods (first and second crops in July, and first crop in October). Untargeted metabolomics integrated with multivariate statistical analysis was employed to deeply investigate the dynamic regulatory influence of harvesting approaches on the accumulation of secondary metabolites. Moreover, the ABTS radical scavenging assay was applied to validate antioxidant capacity and to explore its correlation with compositional characteristics. The results showed that 23 differential metabolites were identified in the samples collected by different harvest methods. Among all the harvest methods, Method 1 yielded the highest number of identified metabolites (53), followed by Method 2 (51) and Method 3 (49), respectively. More importantly, Houttuynoside A, Houttuynoid C, Afzelin, Vitexin and Quercetin were identified as key biomarkers associated with antioxidant capacity, among which Quercetin had the highest correlation with antioxidant capacity.
A method based on UPLC-Q-TOF-MS was established for the systematic characterization and comparison of metabolic profiles and in vitro antioxidant activities in the aerial parts of Houttuynia cordata under different harvest methods (first and second crops in July, and first crop in October). Untargeted metabolomics integrated with multivariate statistical analysis was employed to deeply investigate the dynamic regulatory influence of harvesting approaches on the accumulation of secondary metabolites. Moreover, the ABTS radical scavenging assay was applied to validate antioxidant capacity and to explore its correlation with compositional characteristics. The results showed that 23 differential metabolites were identified in the samples collected by different harvest methods. Among all the harvest methods, Method 1 yielded the highest number of identified metabolites (53), followed by Method 2 (51) and Method 3 (49), respectively. More importantly, Houttuynoside A, Houttuynoid C, Afzelin, Vitexin and Quercetin were identified as key biomarkers associated with antioxidant capacity, among which Quercetin had the highest correlation with antioxidant capacity.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250199
Abstract:
Abiu (Pouteria caimito), an evergreen tree belonging to the genus Pouteria of the Sapotaceae family, boasts high cultivation benefits. However, severe fruit drop under natural conditions significantly undermines its economic value. This study investigated the fruit set characteristics of Abiu cultivars including ‘BaJin’, ‘ShengDa 1’,‘XiongZan’, ‘JiuDa’and ‘DongMi’. The results showed that the mature fruits were mostly borne on the middle and basal parts of the branches, with 1–2 mature fruits per branch on average. Based on these fruit set characteristics, thinning treatments were conducted on the "BaiJin" and "DongMi" cultivars, setting three fruit retention groups: 3 fruits per branch (T3), 2 fruits per branch (T2), and 1 fruit per branch (T1). The effects of fruit thinning on fruit set, fruit development and quality were comprehensively evaluated by determining indicators such as fruit-bearing branch rate, number of mature fruits, fruit transverse and longitudinal diameters, fruit shape index, single fruit weight and fruit quality in different groups. The results indicated that fruit thinning could effectively reduce fruit drop in Abiu. Moreover, the fruit-bearing branch rate and number of mature fruits in the T1 group were significantly higher than those in the T2 and T3 groups. Dynamic measurements of fruit transverse and longitudinal diameters revealed that fruit thinning promoted fruit development; the single fruit weights of "BaiJin" and "DongMi" in the T2 and T1 groups were significantly higher than those in the T3 group. In addition, compared with the T3 group, the soluble solids content and sugar-acid ratio of "BaiJin" increased by 2.99% and 2.7%, respectively, while those of ‘DongMi’ increased by 2.36% and 3.35, respectively, in the T2 group. In conclusion, retaining 1 fruit per branch (T1) through thinning can significantly increase the fruit-bearing branch rate and number of mature fruits, reduce fruit drop, effectively promote fruit development and improve fruit quality, which has important application value in the production of Abiu.
Abiu (Pouteria caimito), an evergreen tree belonging to the genus Pouteria of the Sapotaceae family, boasts high cultivation benefits. However, severe fruit drop under natural conditions significantly undermines its economic value. This study investigated the fruit set characteristics of Abiu cultivars including ‘BaJin’, ‘ShengDa 1’,‘XiongZan’, ‘JiuDa’and ‘DongMi’. The results showed that the mature fruits were mostly borne on the middle and basal parts of the branches, with 1–2 mature fruits per branch on average. Based on these fruit set characteristics, thinning treatments were conducted on the "BaiJin" and "DongMi" cultivars, setting three fruit retention groups: 3 fruits per branch (T3), 2 fruits per branch (T2), and 1 fruit per branch (T1). The effects of fruit thinning on fruit set, fruit development and quality were comprehensively evaluated by determining indicators such as fruit-bearing branch rate, number of mature fruits, fruit transverse and longitudinal diameters, fruit shape index, single fruit weight and fruit quality in different groups. The results indicated that fruit thinning could effectively reduce fruit drop in Abiu. Moreover, the fruit-bearing branch rate and number of mature fruits in the T1 group were significantly higher than those in the T2 and T3 groups. Dynamic measurements of fruit transverse and longitudinal diameters revealed that fruit thinning promoted fruit development; the single fruit weights of "BaiJin" and "DongMi" in the T2 and T1 groups were significantly higher than those in the T3 group. In addition, compared with the T3 group, the soluble solids content and sugar-acid ratio of "BaiJin" increased by 2.99% and 2.7%, respectively, while those of ‘DongMi’ increased by 2.36% and 3.35, respectively, in the T2 group. In conclusion, retaining 1 fruit per branch (T1) through thinning can significantly increase the fruit-bearing branch rate and number of mature fruits, reduce fruit drop, effectively promote fruit development and improve fruit quality, which has important application value in the production of Abiu.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250164
Abstract:
The rapid expansion of rubber plantations has triggered multiple ecological and environmental effects, leading to surface warming through alterations in land surface energy balance and vegetation–atmosphere interactions. Based on multi-source satellite observations, a temperature response model, and the two-resistance mechanism (TRM) framework, the differences in land surface temperature (LST) between natural forests and rubber plantations in the Southeast Asia countries were systematically attributed. The results show that, compared with adjacent natural forests, rubber plantations exhibit an annual mean LST increase of approximately 0.63°C, presenting a consistently “warmer” signal throughout the year. The warming peaks in June and reaches its minimum in July. Mechanistic diagnosis indicates that the increase in aerodynamic resistance (ra) is the dominant driver of surface warming, accounting for more than 60% of the total contribution, which is substantially higher than that of radiative factors such as albedo (α), downward shortwave radiation (S), and downward longwave radiation (L↓). By suppressing latent heat flux and enhancing sensible heat transfer, increased ra elevates surface temperature. In contrast, L↓ exhibits a cooling effect in the attribution framework and jointly regulates surface temperature together with downward shortwave radiation and albedo. Furthermore, under dry and normal moisture conditions, temperature regulation is primarily controlled by ra, whereas under wet conditions, the influence of L↓ becomes more pronounced. These findings reveal the coupled feedback mechanisms among land-use practices, vegetation structure, and regional climate across multiple spatial scales and seasons. The redistribution of radiative and non-radiative fluxes alters surface energy balance and temperature differentials, providing methodological support and a scientific basis for developing region-specific, scenario-sensitive sustainable agricultural policies and land-based climate change mitigation strategies.
The rapid expansion of rubber plantations has triggered multiple ecological and environmental effects, leading to surface warming through alterations in land surface energy balance and vegetation–atmosphere interactions. Based on multi-source satellite observations, a temperature response model, and the two-resistance mechanism (TRM) framework, the differences in land surface temperature (LST) between natural forests and rubber plantations in the Southeast Asia countries were systematically attributed. The results show that, compared with adjacent natural forests, rubber plantations exhibit an annual mean LST increase of approximately 0.63°C, presenting a consistently “warmer” signal throughout the year. The warming peaks in June and reaches its minimum in July. Mechanistic diagnosis indicates that the increase in aerodynamic resistance (ra) is the dominant driver of surface warming, accounting for more than 60% of the total contribution, which is substantially higher than that of radiative factors such as albedo (α), downward shortwave radiation (S), and downward longwave radiation (L↓). By suppressing latent heat flux and enhancing sensible heat transfer, increased ra elevates surface temperature. In contrast, L↓ exhibits a cooling effect in the attribution framework and jointly regulates surface temperature together with downward shortwave radiation and albedo. Furthermore, under dry and normal moisture conditions, temperature regulation is primarily controlled by ra, whereas under wet conditions, the influence of L↓ becomes more pronounced. These findings reveal the coupled feedback mechanisms among land-use practices, vegetation structure, and regional climate across multiple spatial scales and seasons. The redistribution of radiative and non-radiative fluxes alters surface energy balance and temperature differentials, providing methodological support and a scientific basis for developing region-specific, scenario-sensitive sustainable agricultural policies and land-based climate change mitigation strategies.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250122
Abstract:
In recent years, due to the impact of human activities such as large-scale logging and slash-and-burn cultivation, the area of tropical primeval forests has continued to decrease, gradually forming a series of tropical secondary forests at different stages of recovery. However, there is still a lack of clear understanding of the evolution of soil water holding capacity of secondary forests and its dominant regulatory factors at different recovery stages. In this context, soil physical and chemical properties and water-holding performance of tropical secondary forests at three restoration stages (early, middle and late) in Hainan were determined to systematically analyze the effects of different restoration stages on soil water-holding capacity and its internal regulatory mechanism. The results showed that with the progress of restoration, the soil water holding capacity of the (0～60 cm) soil layer increased significantly, and that the natural water content, saturated water holding capacity, capillary water holding capacity and field water holding capacity of the (0～10 cm) soil layer gave a gradual upward trend. At the same time, the total porosity and capillary porosity of the (0～10 cm) soil layer at the late stage of restoration were significantly higher than those at the early and middle stages (P<0.05), while the soil bulk density of the (0～60 cm) soil layer decreased significantly at the late stage of restoration. Soil water holding capacity was positively correlated with total porosity, capillary porosity, non-capillary porosity and organic matter content, and negatively correlated with bulk density. The influence of total porosity was the most significant, with a contribution rate of 26.95%, followed by capillary porosity (23.02%) and bulk density (21.39%). Non-capillary porosity and organic matter also had a certain effect, while the influence of soil particle composition was relatively weak. In conclusion, the restoration of secondary forests significantly enhanced the soil water-holding capacity by improving the pore structure and increasing the content of organic matter, and the effect was most significant at the late stage of restoration.
In recent years, due to the impact of human activities such as large-scale logging and slash-and-burn cultivation, the area of tropical primeval forests has continued to decrease, gradually forming a series of tropical secondary forests at different stages of recovery. However, there is still a lack of clear understanding of the evolution of soil water holding capacity of secondary forests and its dominant regulatory factors at different recovery stages. In this context, soil physical and chemical properties and water-holding performance of tropical secondary forests at three restoration stages (early, middle and late) in Hainan were determined to systematically analyze the effects of different restoration stages on soil water-holding capacity and its internal regulatory mechanism. The results showed that with the progress of restoration, the soil water holding capacity of the (0～60 cm) soil layer increased significantly, and that the natural water content, saturated water holding capacity, capillary water holding capacity and field water holding capacity of the (0～10 cm) soil layer gave a gradual upward trend. At the same time, the total porosity and capillary porosity of the (0～10 cm) soil layer at the late stage of restoration were significantly higher than those at the early and middle stages (P<0.05), while the soil bulk density of the (0～60 cm) soil layer decreased significantly at the late stage of restoration. Soil water holding capacity was positively correlated with total porosity, capillary porosity, non-capillary porosity and organic matter content, and negatively correlated with bulk density. The influence of total porosity was the most significant, with a contribution rate of 26.95%, followed by capillary porosity (23.02%) and bulk density (21.39%). Non-capillary porosity and organic matter also had a certain effect, while the influence of soil particle composition was relatively weak. In conclusion, the restoration of secondary forests significantly enhanced the soil water-holding capacity by improving the pore structure and increasing the content of organic matter, and the effect was most significant at the late stage of restoration.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250121
Abstract:
To address the issue of "white pollution" caused by conventional polyethylene mulch films in open-field watermelon production in tropical regions, fully biodegradable mulch films capable of replacing PE films were screened. Nine biodegradable mulch films made from polybutylene adipate-co-terephthalate (PBAT), lignin, polylactic acid (PLA), polypropylene carbonate (PPC) were employed to mulch the watermelon open fields with conventional PE film as the control (CK) to investigate their application effects on soil water-heat effects, watermelon growth and yield quality, and film biodegradation characteristics. The results indicated that the tested biodegradable films showed no significant difference from CK in terms of heat retention, soil moisture conservation, and promotion of watermelon growth. Of the treatments, Treatment F6 (PBAT + PPC, 0.012 mm) demonstrated the highest fruit edge sugar content (9.53%). Treatment F9 (PBAT+PLA, 0.012 mm) exhibited stable individual melon weight (6.80 kg) and yield per unit area (136.04 t·hm−2), slightly outperforming the CK. Mulch degradation observations revealed that among all the fully biodegradable films, Treatment F9 exhibited the slowest degradation rate, second only to the CK. Regarding costs, the total cost per mu for Treatments F6 and F9 (approximately ¥275) increased by about 4.6% compared to CK (approximately ¥263). In summary, the 0.012 mm thick films in Treatments F6 and F9 demonstrate promising potential for replacing conventional PE films in open-field watermelon production in tropical regions. Their degradation cycles align well with the watermelon growth period, maintaining stable soil conditions at the critical growth stages.
To address the issue of "white pollution" caused by conventional polyethylene mulch films in open-field watermelon production in tropical regions, fully biodegradable mulch films capable of replacing PE films were screened. Nine biodegradable mulch films made from polybutylene adipate-co-terephthalate (PBAT), lignin, polylactic acid (PLA), polypropylene carbonate (PPC) were employed to mulch the watermelon open fields with conventional PE film as the control (CK) to investigate their application effects on soil water-heat effects, watermelon growth and yield quality, and film biodegradation characteristics. The results indicated that the tested biodegradable films showed no significant difference from CK in terms of heat retention, soil moisture conservation, and promotion of watermelon growth. Of the treatments, Treatment F6 (PBAT + PPC, 0.012 mm) demonstrated the highest fruit edge sugar content (9.53%). Treatment F9 (PBAT+PLA, 0.012 mm) exhibited stable individual melon weight (6.80 kg) and yield per unit area (136.04 t·hm−2), slightly outperforming the CK. Mulch degradation observations revealed that among all the fully biodegradable films, Treatment F9 exhibited the slowest degradation rate, second only to the CK. Regarding costs, the total cost per mu for Treatments F6 and F9 (approximately ¥275) increased by about 4.6% compared to CK (approximately ¥263). In summary, the 0.012 mm thick films in Treatments F6 and F9 demonstrate promising potential for replacing conventional PE films in open-field watermelon production in tropical regions. Their degradation cycles align well with the watermelon growth period, maintaining stable soil conditions at the critical growth stages.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250135
Abstract:
As the dominant groups in saline ecosystems,halophytes have been systematically studied in their salt tolerance mechanisms at the physiological, biochemical, molecular and metabolomic levels. In recent years, the issue of combined soil heavy metal-saline-alkali pollution has become increasingly severe, drawing significant attention to halophytes due to their cross-tolerance to multiple stresses. Research indicates that halophytes precisely regulate heavy metal uptake, compartmentalization, and efflux through specific ion transport systems such as HMA. They achieve synergistic detoxification via antioxidant defenses and chelation mechanisms (e.g., plant chelating peptides, metallothionein). It is interesting to note that salinity and heavy metal stresses share epigenetic regulatory mechanisms—including MAPK signaling cascades, ABA/JA hormone pathways, thereby establishing the molecular basis for cross-tolerance. Therefore, halophytes are not only adapted to high-salinity environments but also possess the capacity to accumulate or stabilise heavy metals in soil, presenting a green solution for concurrently addressing salinisation and heavy metal contamination. This paper systematically reviews the mechanisms underlying the cross-tolerance traits of halophytes. Drawing upon representative case studies, it examines their remediation potential from multiple perspectives, including restoration principles and molecular breeding improvement strategies, aiming to provide theoretical foundations for the green and sustainable management of combined saline-alkali and heavy metal pollution.
As the dominant groups in saline ecosystems,halophytes have been systematically studied in their salt tolerance mechanisms at the physiological, biochemical, molecular and metabolomic levels. In recent years, the issue of combined soil heavy metal-saline-alkali pollution has become increasingly severe, drawing significant attention to halophytes due to their cross-tolerance to multiple stresses. Research indicates that halophytes precisely regulate heavy metal uptake, compartmentalization, and efflux through specific ion transport systems such as HMA. They achieve synergistic detoxification via antioxidant defenses and chelation mechanisms (e.g., plant chelating peptides, metallothionein). It is interesting to note that salinity and heavy metal stresses share epigenetic regulatory mechanisms—including MAPK signaling cascades, ABA/JA hormone pathways, thereby establishing the molecular basis for cross-tolerance. Therefore, halophytes are not only adapted to high-salinity environments but also possess the capacity to accumulate or stabilise heavy metals in soil, presenting a green solution for concurrently addressing salinisation and heavy metal contamination. This paper systematically reviews the mechanisms underlying the cross-tolerance traits of halophytes. Drawing upon representative case studies, it examines their remediation potential from multiple perspectives, including restoration principles and molecular breeding improvement strategies, aiming to provide theoretical foundations for the green and sustainable management of combined saline-alkali and heavy metal pollution.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250156
Abstract:
Vibrio alginolyticus, a conditionally pathogenic bacterium widely present in marine environments, can utilize various inorganic nitrogen sources such as ammonium, nitrate, and nitrite for growth, causing significant economic losses in the marine aquaculture industry. The nirB gene encodes nitrite reductase, which plays an important role in bacterial nitrogen metabolism and environmental adaptation. An attempt was made to analyze the function of the nirB gene in V. alginolyticus during the utilization of inorganic nitrogen sources. Two nirB gene-related knockout strains of V. alginolyticus were constructed: a nirB1 deletion strain (ΔnirB1) and a nirB2 deletion strain (ΔnirB2), and a double deletion strain lacking both nirB1 and nirB2 (ΔnirB12) was also constructed. These strains were cultured in different types of inorganic nitrogen media to observe their growth. The results showed that the nirB gene promotes the utilization of ammonium and nitrate in V. alginolyticus, with the nirB1 gene playing a key role in the conversion of nitrate to nitrite. In nitrite media, deletion of the nirB gene significantly inhibited the growth of V. alginolyticus, indicating that the gene also plays an important role in nitrite utilization, and the nirB2 gene has a more critical function in this process. Moreover, the presence of ammonium was also found to affect the utilization of nitrite by V. alginolyticus, suggesting a possible competitive relationship. All the results provide a foundation for further understanding of the function of the nirB gene in V. alginolyticus and its role in nitrogen metabolism.
Vibrio alginolyticus, a conditionally pathogenic bacterium widely present in marine environments, can utilize various inorganic nitrogen sources such as ammonium, nitrate, and nitrite for growth, causing significant economic losses in the marine aquaculture industry. The nirB gene encodes nitrite reductase, which plays an important role in bacterial nitrogen metabolism and environmental adaptation. An attempt was made to analyze the function of the nirB gene in V. alginolyticus during the utilization of inorganic nitrogen sources. Two nirB gene-related knockout strains of V. alginolyticus were constructed: a nirB1 deletion strain (ΔnirB1) and a nirB2 deletion strain (ΔnirB2), and a double deletion strain lacking both nirB1 and nirB2 (ΔnirB12) was also constructed. These strains were cultured in different types of inorganic nitrogen media to observe their growth. The results showed that the nirB gene promotes the utilization of ammonium and nitrate in V. alginolyticus, with the nirB1 gene playing a key role in the conversion of nitrate to nitrite. In nitrite media, deletion of the nirB gene significantly inhibited the growth of V. alginolyticus, indicating that the gene also plays an important role in nitrite utilization, and the nirB2 gene has a more critical function in this process. Moreover, the presence of ammonium was also found to affect the utilization of nitrite by V. alginolyticus, suggesting a possible competitive relationship. All the results provide a foundation for further understanding of the function of the nirB gene in V. alginolyticus and its role in nitrogen metabolism.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250176
Abstract:
Based on forest resource data of Hainan Province from the third to the ninth national forest resource inventories and relevant ecological station data from the same period, the dynamic characteristics of forest resources over more than 30 years (1984–2018) were systematically analyzed in terms of "quantity-quality-structure", and the evolutionary patterns of key ecosystem service functions were quantified in accordance with national standards, aiming to reveal their intrinsic coupling relationships. The results showed that forest resources in Hainan Province had achieved remarkable growth, with forest area, stock volume, and forest coverage increasing by 124.48%, 163.84%, and 31.96 percentage points, respectively. The forest age structure had been continuously optimized, and forest quality had steadily improved, with policy and institutional factors identified as key drivers of these changes. All assessed ecosystem service functions had significantly enhanced, particularly water regulation and negative ion provision, which had increased by over 240%. Correlation analysis further revealed that the area of mature and overmature forests had the greatest dominant effect on water conservation and soil fixation services, while stock volume per unit area was the key determining factor for carbon sequestration service. This highlighted the central role of forest quality and maturity in enhancing ecosystem services, underscoring the central importance of indicators of forest quantity (area, stock volume) and indicators of maturity. All these findings elucidate the coupling relationship between the growth of forest resources and the dynamic changes in ecosystem service functions, providing a scientific basis for the sustainable management of regional forests.
Based on forest resource data of Hainan Province from the third to the ninth national forest resource inventories and relevant ecological station data from the same period, the dynamic characteristics of forest resources over more than 30 years (1984–2018) were systematically analyzed in terms of "quantity-quality-structure", and the evolutionary patterns of key ecosystem service functions were quantified in accordance with national standards, aiming to reveal their intrinsic coupling relationships. The results showed that forest resources in Hainan Province had achieved remarkable growth, with forest area, stock volume, and forest coverage increasing by 124.48%, 163.84%, and 31.96 percentage points, respectively. The forest age structure had been continuously optimized, and forest quality had steadily improved, with policy and institutional factors identified as key drivers of these changes. All assessed ecosystem service functions had significantly enhanced, particularly water regulation and negative ion provision, which had increased by over 240%. Correlation analysis further revealed that the area of mature and overmature forests had the greatest dominant effect on water conservation and soil fixation services, while stock volume per unit area was the key determining factor for carbon sequestration service. This highlighted the central role of forest quality and maturity in enhancing ecosystem services, underscoring the central importance of indicators of forest quantity (area, stock volume) and indicators of maturity. All these findings elucidate the coupling relationship between the growth of forest resources and the dynamic changes in ecosystem service functions, providing a scientific basis for the sustainable management of regional forests.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250182
Abstract:
Vatica mangachapoi Blanco, a member of the genus Vatica (Dipterocarpaceae), is a dominant species in the tropical lowland rainforests of Hainan Island. Understanding its genetic dynamics and fine-scale gene dispersal patterns is essential for elucidating the ecological adaptation of dominant tree species in tropical ecosystems. In this context 372 individuals across different elevational gradients were sampled from Bawangling and Diaoluoshan within Hainan Tropical Rainforest National Park to evaluate genetic diversity, population structure, and fine-scale spatial genetic structure (FSGS) using seven microsatellite loci. The results revealed a relatively high overall genetic diversity in V. mangachapoi (Na = 7.107, He = 0.726), markedly higher than that of other two dipterocarps on Hainan Island, Hopea hainanensis (Na = 2.458, He = 0.432) and H. reticulata (Na = 3.636, He = 0.599). STRUCTURE analysis found that the high altitude population at Bawangling (BW-H) differentiated to some extent with the other three populations, while PCoA and NJ tree analyses could not detect clear genetic differentiation among the four populations. FSGS analyses indicated stronger spatial clustering of genotypes in high-elevation populations (Sp =0.0360 -0.0440 ) than that in low-elevation populations (Sp = 0.0096 -0.0168 ). Overall, all the findings indicate that the genetic dynamics of V. mangachapoi populations are influenced by elevational environmental gradients and provide scientific insights for the conservation and management of tropical dipterocarps.
Vatica mangachapoi Blanco, a member of the genus Vatica (Dipterocarpaceae), is a dominant species in the tropical lowland rainforests of Hainan Island. Understanding its genetic dynamics and fine-scale gene dispersal patterns is essential for elucidating the ecological adaptation of dominant tree species in tropical ecosystems. In this context 372 individuals across different elevational gradients were sampled from Bawangling and Diaoluoshan within Hainan Tropical Rainforest National Park to evaluate genetic diversity, population structure, and fine-scale spatial genetic structure (FSGS) using seven microsatellite loci. The results revealed a relatively high overall genetic diversity in V. mangachapoi (Na = 7.107, He = 0.726), markedly higher than that of other two dipterocarps on Hainan Island, Hopea hainanensis (Na = 2.458, He = 0.432) and H. reticulata (Na = 3.636, He = 0.599). STRUCTURE analysis found that the high altitude population at Bawangling (BW-H) differentiated to some extent with the other three populations, while PCoA and NJ tree analyses could not detect clear genetic differentiation among the four populations. FSGS analyses indicated stronger spatial clustering of genotypes in high-elevation populations (Sp =
, Available online , doi: 10.15886/j.cnki.rdswxb.20250179
Abstract:
To address the issues of high cost and low yield in the cultivation of Dictyophora rubrovolvata in Hainan, this study conducted a gradient experiment to optimize the mushroom-stick substrate and casing soil composition. Key parameters, including mycelial growth rate, bag-filling time, primordia formation time, fruiting time, fruiting body morphological characteristics, and yield, were measured to evaluate the effects of different carbon sources, nitrogen sources, and organic matter on the growth of D. rubrovolvata. The results indicated that the A5 substrates formula, containing rubberwood as the carbon source and soybean as the nitrogen source, exhibited the best performance. It achieved a mycelial growth rate of 12.62 mm·d-1, a bag-filling time of 16.33 d, and produced dense, robust, and pure white mycelia. The addition of sphagnum peat to the casing soil at varying ratios enhanced mycelial growth, shortened the time to primordia formation and fruiting, and increased yield. Specifically, the highest yield per stick (93.76 g) were achieved with the application of 20% peat to the base soil from Guangcun Town. The application of 30% peat to the base soil from Dacheng Town resulted in the highest yield per stick (103.58 g) and. Similarly, the addition of 30% peat to the Xinzhou Town base soil yielded at 92.66 g per stick This study provides a theoretical basis and technical support for the efficient and low-cost cultivation of D. rubrovolvata in tropical regions.
To address the issues of high cost and low yield in the cultivation of Dictyophora rubrovolvata in Hainan, this study conducted a gradient experiment to optimize the mushroom-stick substrate and casing soil composition. Key parameters, including mycelial growth rate, bag-filling time, primordia formation time, fruiting time, fruiting body morphological characteristics, and yield, were measured to evaluate the effects of different carbon sources, nitrogen sources, and organic matter on the growth of D. rubrovolvata. The results indicated that the A5 substrates formula, containing rubberwood as the carbon source and soybean as the nitrogen source, exhibited the best performance. It achieved a mycelial growth rate of 12.62 mm·d-1, a bag-filling time of 16.33 d, and produced dense, robust, and pure white mycelia. The addition of sphagnum peat to the casing soil at varying ratios enhanced mycelial growth, shortened the time to primordia formation and fruiting, and increased yield. Specifically, the highest yield per stick (93.76 g) were achieved with the application of 20% peat to the base soil from Guangcun Town. The application of 30% peat to the base soil from Dacheng Town resulted in the highest yield per stick (103.58 g) and. Similarly, the addition of 30% peat to the Xinzhou Town base soil yielded at 92.66 g per stick This study provides a theoretical basis and technical support for the efficient and low-cost cultivation of D. rubrovolvata in tropical regions.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250147
Abstract:
During the field survey in Jianfengling district of Hainan Tropical Rainforest National Park, four plant species newly recorded in Hainan Province were reported, including: Viola nanlingensis J. S. Zhou & F. W. Xing, Capparis tenera Dalz and Primulina flavimaculata (W. T. Wang) Mich. Möller & A. Weber, Amorphophallus tonkinensis Engl. & Gehrm. For each species, field photographs were provided along with descriptions of their morphological characteristics. Additionally, the gaps in the original literature and the Flora of China regarding the fruit and seed descriptions of Capparis tenera were supplemented. Voucher specimens are deposited in the Shanghai Chenshan National Botanical Garden Herbarium (CSH) and the South China Botanical Garden Herbarium (IBSC). Furthermore, a correction was made regarding the 1981 publication by Chen Bangyu in the Acta Phytotaxonomica Sinica, which proposed two new species of Engelhardia from Hainan and stated that "Engelhardia spicata does not occur in Hainan, and that the plants previously misidentified as Engelhardia spicata should be recognized as a new species, Engelhardia hainanensis." It has now been confirmed that Engelhardia spicata does indeed exist in Hainan, with voucher specimens deposited in the South China Botanical Garden Herbarium (IBSC).
During the field survey in Jianfengling district of Hainan Tropical Rainforest National Park, four plant species newly recorded in Hainan Province were reported, including: Viola nanlingensis J. S. Zhou & F. W. Xing, Capparis tenera Dalz and Primulina flavimaculata (W. T. Wang) Mich. Möller & A. Weber, Amorphophallus tonkinensis Engl. & Gehrm. For each species, field photographs were provided along with descriptions of their morphological characteristics. Additionally, the gaps in the original literature and the Flora of China regarding the fruit and seed descriptions of Capparis tenera were supplemented. Voucher specimens are deposited in the Shanghai Chenshan National Botanical Garden Herbarium (CSH) and the South China Botanical Garden Herbarium (IBSC). Furthermore, a correction was made regarding the 1981 publication by Chen Bangyu in the Acta Phytotaxonomica Sinica, which proposed two new species of Engelhardia from Hainan and stated that "Engelhardia spicata does not occur in Hainan, and that the plants previously misidentified as Engelhardia spicata should be recognized as a new species, Engelhardia hainanensis." It has now been confirmed that Engelhardia spicata does indeed exist in Hainan, with voucher specimens deposited in the South China Botanical Garden Herbarium (IBSC).
, Available online , doi: 10.15886/j.cnki.rdswxb.20250145
Abstract:
Grassland ecosystems are an important component of terrestrial ecosystems and play a critical role in maintaining ecological security and supporting livestock production. Due to climate warming and overgrazing, grassland productivity has declined, increasing the risk of ecological degradation. Existing studies on grazing intensity and grazing thresholds largely rely on empirical or static data, making it difficult to capture the dynamic changes of grasslands under grazing pressure. This study takes the grasslands on the northern Tianshan Mountains as an example, using LAI time series unaffected by grazing to simulate ungrazed growth trajectories and applying a light-use efficiency model to calculate ungrazed net primary productivity (NPP). Based on this, a grazing threshold model was established using ungrazed NPP to describe degradation time and threshold distribution under different grazing pressures. The results show that the spatial distribution of ungrazed NPP exhibits a “higher in the west and in mountainous areas, lower in the east and on plains” pattern. Under current stocking rates and scenarios with 10% and 30% reduced grazing, grassland degradation generally occurs within 1–18 years, with some areas reaching a critical stocking rate of zero, indicating that current grazing pressure has reached or exceeded the ecological carrying limit. As stocking rates decrease, the time for some pixels to reach the degradation threshold is significantly delayed, suggesting that moderate grazing reduction can locally postpone grassland degradation. Under the RCP4.5 and RCP8.5 scenarios, the critical stocking rate ranges from 3.59 to 9.60 SU/ha, with limited overall differences in grazing thresholds and degradation time, but notable regional variability. The proposed ungrazed NPP–based grazing threshold simulation approach provides a scientific basis for quantitatively identifying grassland ecological carrying capacity and supporting differentiated grazing management strategies.
Grassland ecosystems are an important component of terrestrial ecosystems and play a critical role in maintaining ecological security and supporting livestock production. Due to climate warming and overgrazing, grassland productivity has declined, increasing the risk of ecological degradation. Existing studies on grazing intensity and grazing thresholds largely rely on empirical or static data, making it difficult to capture the dynamic changes of grasslands under grazing pressure. This study takes the grasslands on the northern Tianshan Mountains as an example, using LAI time series unaffected by grazing to simulate ungrazed growth trajectories and applying a light-use efficiency model to calculate ungrazed net primary productivity (NPP). Based on this, a grazing threshold model was established using ungrazed NPP to describe degradation time and threshold distribution under different grazing pressures. The results show that the spatial distribution of ungrazed NPP exhibits a “higher in the west and in mountainous areas, lower in the east and on plains” pattern. Under current stocking rates and scenarios with 10% and 30% reduced grazing, grassland degradation generally occurs within 1–18 years, with some areas reaching a critical stocking rate of zero, indicating that current grazing pressure has reached or exceeded the ecological carrying limit. As stocking rates decrease, the time for some pixels to reach the degradation threshold is significantly delayed, suggesting that moderate grazing reduction can locally postpone grassland degradation. Under the RCP4.5 and RCP8.5 scenarios, the critical stocking rate ranges from 3.59 to 9.60 SU/ha, with limited overall differences in grazing thresholds and degradation time, but notable regional variability. The proposed ungrazed NPP–based grazing threshold simulation approach provides a scientific basis for quantitatively identifying grassland ecological carrying capacity and supporting differentiated grazing management strategies.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250111
Abstract:
The umbrella species theory emphasizes that protecting a focal species and its habitat can indirectly benefit co-occurring species within the same ecosystem. The Hainan gibbon (Nomascus hainanus), one of the most endangered primates worldwide, is restricted to the Bawangling area of Hainan Tropical Rainforest National Park and is recognized as both a flagship species and a potential umbrella species. Based on camera-trapping data, the biodiversity patterns were compared between the gibbon-inhabited Bawangling area and the adjacent Yajialing area without gibbons, aiming to evaluate the umbrella effect of the Hainan gibbon. Results showed that although the two regions share similar geographic location, elevation, and vegetation types, their species composition and community structure differ significantly. A total of 47 bird and mammal species were recorded in Bawangling, compared with 40 species in Yajiadaling. Species accumulation curves indicated that species richness in both areas tended to stabilize over time, yet Bawangling consistently exhibited higher richness and evenness. Furthermore, the higher occurrence frequencies of resource competitors and potential predators in Bawangling suggest more complex interspecific interactions and greater ecosystem robustness. Overall, our findings confirm the umbrella effect of the Hainan gibbon: its distribution area harbors not only higher biodiversity but also a more complete community structure and ecological functionality, providing empirical support for applying the umbrella species concept in tropical island ecosystems.
The umbrella species theory emphasizes that protecting a focal species and its habitat can indirectly benefit co-occurring species within the same ecosystem. The Hainan gibbon (Nomascus hainanus), one of the most endangered primates worldwide, is restricted to the Bawangling area of Hainan Tropical Rainforest National Park and is recognized as both a flagship species and a potential umbrella species. Based on camera-trapping data, the biodiversity patterns were compared between the gibbon-inhabited Bawangling area and the adjacent Yajialing area without gibbons, aiming to evaluate the umbrella effect of the Hainan gibbon. Results showed that although the two regions share similar geographic location, elevation, and vegetation types, their species composition and community structure differ significantly. A total of 47 bird and mammal species were recorded in Bawangling, compared with 40 species in Yajiadaling. Species accumulation curves indicated that species richness in both areas tended to stabilize over time, yet Bawangling consistently exhibited higher richness and evenness. Furthermore, the higher occurrence frequencies of resource competitors and potential predators in Bawangling suggest more complex interspecific interactions and greater ecosystem robustness. Overall, our findings confirm the umbrella effect of the Hainan gibbon: its distribution area harbors not only higher biodiversity but also a more complete community structure and ecological functionality, providing empirical support for applying the umbrella species concept in tropical island ecosystems.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250065
Abstract:
Coral reef ecosystem is one of the most biodiverse and productive ecosystems in the ocean．However, coral reefs have been degraded to different degrees in recent years due to the impact of global environmental changes．As microorganisms that live in symbiosis with corals, the isolation and purification of Symbiodiniaceae clades can provide a deeper understanding of their biological properties and provide an experimental basis for revealing their important physiology, such as coral-associated physiology mechanisms．In this context Cladocopium C1 sp．was isolated from Acropora formosa, and then rinsed or sheared to make the coral released symbiotic Symbiodiniaceae．Observations were made using an inverted microscope microscope to determine whether Symbiodiniaceae were isolated or not, and the Symbiodiniaceae were centrifuged, diluted, transferred, and cultured every two weeks．Sanger sequencing and high-throughput sequencing were performed on the cultured Symbiodiniaceae．Observations under inverted microscope and electron microscope showed that the cells of the Symbiodiniaceae Cladocopium C1 sp．had a complete structure with multiple well-developed chlorophylls, mitochondria, pyrenoids, etc in the cytoplasm．The Sanger sequencing results were compared on the NCBI platform, and the isolated Symbiodiniaceae were the dominant Symbiodiniaceae Cladocopium C1 sp．of A．formosa．The high-throughput sequencing results showed that the relative abundance of Symbiodiniaceae Cladocopium C1 sp．cells was 98%．The successful isolation of Cladocopium C1 sp．from A．formosa provides a scientific basis for a deeper understanding of the roles of Symbiodiniaceae in the symbiosis with corals and the mechanism of their interactions with corals.
Coral reef ecosystem is one of the most biodiverse and productive ecosystems in the ocean．However, coral reefs have been degraded to different degrees in recent years due to the impact of global environmental changes．As microorganisms that live in symbiosis with corals, the isolation and purification of Symbiodiniaceae clades can provide a deeper understanding of their biological properties and provide an experimental basis for revealing their important physiology, such as coral-associated physiology mechanisms．In this context Cladocopium C1 sp．was isolated from Acropora formosa, and then rinsed or sheared to make the coral released symbiotic Symbiodiniaceae．Observations were made using an inverted microscope microscope to determine whether Symbiodiniaceae were isolated or not, and the Symbiodiniaceae were centrifuged, diluted, transferred, and cultured every two weeks．Sanger sequencing and high-throughput sequencing were performed on the cultured Symbiodiniaceae．Observations under inverted microscope and electron microscope showed that the cells of the Symbiodiniaceae Cladocopium C1 sp．had a complete structure with multiple well-developed chlorophylls, mitochondria, pyrenoids, etc in the cytoplasm．The Sanger sequencing results were compared on the NCBI platform, and the isolated Symbiodiniaceae were the dominant Symbiodiniaceae Cladocopium C1 sp．of A．formosa．The high-throughput sequencing results showed that the relative abundance of Symbiodiniaceae Cladocopium C1 sp．cells was 98%．The successful isolation of Cladocopium C1 sp．from A．formosa provides a scientific basis for a deeper understanding of the roles of Symbiodiniaceae in the symbiosis with corals and the mechanism of their interactions with corals.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250197
Abstract:
Anther connation is an important floral trait that influences pollination accuracy and efficiency by altering anther arrangement and anther-stigma separation (herkogamy). The flowering biology, the mechanical properties of connate anthers, and the pollination process of Aeschynanthus moningeriae (Gesneriaceae)at Mt. Wuzhi in Hainan Island, China were examined to investigate the developmental pattern of anther connation and pollination adaptation in the bird-pollinated A. moningeriae. The results showed that A. moningeriae is hermaphroditic, with didynamous stamens containing anthers in pairs from the bud stage and that anthers were coherent throughout the whole flowering period. The long anther pair ultimately formed an angle of approximately 180° (parallel) while the short anther pair grow at 115° (inverted V-shape). The short anther pair was connated with more fused part than the long anther pair. The mechanical strength of the short anther pair was F=0.159 ± 0.031 N and F=0.211 ± 0.034 N in the upward and downward directions respectively, which is significantly higher than that of the long anther pair (upward: F=0.127 ± 0.020 N; downward: F=0.117 ± 0.018 N). The fork-tailed sunbird (Aethopyga latouchii) was the only effective pollinator. The red corolla, along with the black stripes on the lobes, function as a nectar guide. The long anther pair was mainly touched by the bird forehead, while the short anther pair was contacted mainly by the narrow base of the bird beak. Such separated pollen deposition increased the contact probobality of the stigma with pollen. Because the bird beak exerted stronger force on anthers, the higher mechanical strength with higher fusion proportion of the short anther pair can thus maintain at a fusion mode and spatial position of the connate anthers under stronger force, increasing pollen removal ratio.
Anther connation is an important floral trait that influences pollination accuracy and efficiency by altering anther arrangement and anther-stigma separation (herkogamy). The flowering biology, the mechanical properties of connate anthers, and the pollination process of Aeschynanthus moningeriae (Gesneriaceae)at Mt. Wuzhi in Hainan Island, China were examined to investigate the developmental pattern of anther connation and pollination adaptation in the bird-pollinated A. moningeriae. The results showed that A. moningeriae is hermaphroditic, with didynamous stamens containing anthers in pairs from the bud stage and that anthers were coherent throughout the whole flowering period. The long anther pair ultimately formed an angle of approximately 180° (parallel) while the short anther pair grow at 115° (inverted V-shape). The short anther pair was connated with more fused part than the long anther pair. The mechanical strength of the short anther pair was F=0.159 ± 0.031 N and F=0.211 ± 0.034 N in the upward and downward directions respectively, which is significantly higher than that of the long anther pair (upward: F=0.127 ± 0.020 N; downward: F=0.117 ± 0.018 N). The fork-tailed sunbird (Aethopyga latouchii) was the only effective pollinator. The red corolla, along with the black stripes on the lobes, function as a nectar guide. The long anther pair was mainly touched by the bird forehead, while the short anther pair was contacted mainly by the narrow base of the bird beak. Such separated pollen deposition increased the contact probobality of the stigma with pollen. Because the bird beak exerted stronger force on anthers, the higher mechanical strength with higher fusion proportion of the short anther pair can thus maintain at a fusion mode and spatial position of the connate anthers under stronger force, increasing pollen removal ratio.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250159
Abstract:
To understand the resource status of aquatic plants in Hainan Tropical Rainforest National Park, the species composition and floristic characteristics of aquatic plants in the park were analyzed based on field surveys and literature review. The results show that a total of 468 species of aquatic plants belonging to 255 genera and 95 families were recorded, among which angiosperms were absolutely dominant with 432 species. Both family- and genus-level species richness showed a "core–periphery" distribution pattern, i.e., species were highly concentrated in a few dominant families and genera. In terms of growth form, herbaceous plants predominated; regarding life form, perennials were the majority; and concerning ecological type, helophytes–emergent plants were dominant. There were 11 key protected wild plants, 26 Chinese endemic species, and 13 threatened plants. A total of 57 alien invasive plant species were recorded, among which harmful invasive species accounted for up to 80.70%, mostly originating from the Americas. The floristic composition at both family and genus levels exhibited strong tropical affinities, with the R/T ratio at the genus level reaching 8.95. All these findings indicate that the aquatic plants in Hainan Tropical Rainforest National Park are distinctly tropical and of high conservation value, yet they face threats from multiple alien invasive species. It is recommended to strengthen the conservation of rare species and the prevention and control of invasive plants.
To understand the resource status of aquatic plants in Hainan Tropical Rainforest National Park, the species composition and floristic characteristics of aquatic plants in the park were analyzed based on field surveys and literature review. The results show that a total of 468 species of aquatic plants belonging to 255 genera and 95 families were recorded, among which angiosperms were absolutely dominant with 432 species. Both family- and genus-level species richness showed a "core–periphery" distribution pattern, i.e., species were highly concentrated in a few dominant families and genera. In terms of growth form, herbaceous plants predominated; regarding life form, perennials were the majority; and concerning ecological type, helophytes–emergent plants were dominant. There were 11 key protected wild plants, 26 Chinese endemic species, and 13 threatened plants. A total of 57 alien invasive plant species were recorded, among which harmful invasive species accounted for up to 80.70%, mostly originating from the Americas. The floristic composition at both family and genus levels exhibited strong tropical affinities, with the R/T ratio at the genus level reaching 8.95. All these findings indicate that the aquatic plants in Hainan Tropical Rainforest National Park are distinctly tropical and of high conservation value, yet they face threats from multiple alien invasive species. It is recommended to strengthen the conservation of rare species and the prevention and control of invasive plants.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250136
Abstract:
Hainan Tropical Rainforest National Park is the only national park in China with an expressway traversing the Park. To reveal possible potential impacts of the expressway section crossing the National Park, three sites (Zayun, Maoyang, Fanyang) along the expressway section were selected to determine the soil heavy metal (Cr, Ni, Pb, Cu and Zn) contents and analyze the distribution pattern of the heavy metals on both sides of the expressway section. The main sources and affecting factors of each heavy metal were also examined. The results showed that Cr and Ni were the main heavy metals with the highest accumulative rate in the soil, particularly at Zayun and Maoyang, where the soil Cr content exceeded that of the control by 100% and 83.33%, respectively. Soil heavy metal content showed a decreasing trend with increased distance from the expressway or an increasing and then decreasing trend, with the peak appearing within 0-10 m from the expressway. The Geo-accumulation Index showed that the soil Cr, Ni and Zn were at the level of non-pollution or low pollution and thus the overall ecological risk was low. The main source of the soil Cr was traffic emission, while the soil Pb, Cu and Zn were mainly affected by the parent material, with the soil Ni showing mixed sources. All these results suggested that the current ecological risk of soil heavy metal along the expressway section was low, but the soil Cr and Ni were gradually accumulated in the soil near the expressway. It is recommended to strengthen the vegetation restoration and management of the expressway section, to improve the measures for collection and treatment of the expressway runoffs, and to focus on monitoring the dynamics at ecological sensitive sites such as Changhua River, which are crucial for preventing the potential accumulation and migration of soil heavy metals and to ensure the ecological security and high-quality construction of Hainan Tropical Rainforest National Park.
Hainan Tropical Rainforest National Park is the only national park in China with an expressway traversing the Park. To reveal possible potential impacts of the expressway section crossing the National Park, three sites (Zayun, Maoyang, Fanyang) along the expressway section were selected to determine the soil heavy metal (Cr, Ni, Pb, Cu and Zn) contents and analyze the distribution pattern of the heavy metals on both sides of the expressway section. The main sources and affecting factors of each heavy metal were also examined. The results showed that Cr and Ni were the main heavy metals with the highest accumulative rate in the soil, particularly at Zayun and Maoyang, where the soil Cr content exceeded that of the control by 100% and 83.33%, respectively. Soil heavy metal content showed a decreasing trend with increased distance from the expressway or an increasing and then decreasing trend, with the peak appearing within 0-10 m from the expressway. The Geo-accumulation Index showed that the soil Cr, Ni and Zn were at the level of non-pollution or low pollution and thus the overall ecological risk was low. The main source of the soil Cr was traffic emission, while the soil Pb, Cu and Zn were mainly affected by the parent material, with the soil Ni showing mixed sources. All these results suggested that the current ecological risk of soil heavy metal along the expressway section was low, but the soil Cr and Ni were gradually accumulated in the soil near the expressway. It is recommended to strengthen the vegetation restoration and management of the expressway section, to improve the measures for collection and treatment of the expressway runoffs, and to focus on monitoring the dynamics at ecological sensitive sites such as Changhua River, which are crucial for preventing the potential accumulation and migration of soil heavy metals and to ensure the ecological security and high-quality construction of Hainan Tropical Rainforest National Park.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250137
Abstract:
Hainan Island, as one of the global biodiversity hotspots, has its biodiversity concentrated in National Park of Hainan Tropical Rainforest. Primulina heterotricha, an endemic plant of Hainan Island, has a widespread distribution and consists of two floral color morphs: yellow-flowered and purple-flowered individuals. First, to clarify the differentiation pattern of floral traits among different geographical populations, we measured key floral traits including flower size, floral mouth width, and corolla tube length across various geographical populations, and analyzed the potential driving effect of geographical isolation on the differentiation of these floral traits. Second, focusing on the differentiation of two floral color morphs and the differences in pollination mechanisms, we comparatively analyzed the flowering dynamics of yellow-flowered and purple-flowered individuals, as well as flower-visiting insects and their visiting behaviors, to reveal the possible differences in pollination mechanisms between populations with different flower colors. A multivariate analysis of variance (MANOVA) revealed significant differences in floral traits among different geographical populations (P < 0.05), but geographical distance and altitude had no significant effect on floral traits. Populations with different floral colors also did not show significant difference in floral traits (P > 0.05). Moreover, at different flowering stages, there were no significant differences in stamen length, pistil length and herkogamy degree between the two floral color populations (P > 0.05), but the stigma gradually elongated and exceeded the position of the anthers as the flowering period progressed. Observations on flower-visiting insects indicated that the main flower-visiting insects of yellow-flowered populations were Amegilla leptocoma and A. yunnanensis, while the main flower-visiting insect of the purple-flowered populations was A. leptocoma. Thus, yellow-flowered individuals have a more generalized assemblage of flower visitors, whereas purple-flowered individuals exhibit a more specialized pollination system. This study investigated the differentiation pattern of floral traits among different geographical populations, floral color diversity and pollination adaptation process of P. heterotricha, and provides a scientific basis for biodiversity conservation and pollination service function assessment in National Park of Hainan Tropical Rainforest..
Hainan Island, as one of the global biodiversity hotspots, has its biodiversity concentrated in National Park of Hainan Tropical Rainforest. Primulina heterotricha, an endemic plant of Hainan Island, has a widespread distribution and consists of two floral color morphs: yellow-flowered and purple-flowered individuals. First, to clarify the differentiation pattern of floral traits among different geographical populations, we measured key floral traits including flower size, floral mouth width, and corolla tube length across various geographical populations, and analyzed the potential driving effect of geographical isolation on the differentiation of these floral traits. Second, focusing on the differentiation of two floral color morphs and the differences in pollination mechanisms, we comparatively analyzed the flowering dynamics of yellow-flowered and purple-flowered individuals, as well as flower-visiting insects and their visiting behaviors, to reveal the possible differences in pollination mechanisms between populations with different flower colors. A multivariate analysis of variance (MANOVA) revealed significant differences in floral traits among different geographical populations (P < 0.05), but geographical distance and altitude had no significant effect on floral traits. Populations with different floral colors also did not show significant difference in floral traits (P > 0.05). Moreover, at different flowering stages, there were no significant differences in stamen length, pistil length and herkogamy degree between the two floral color populations (P > 0.05), but the stigma gradually elongated and exceeded the position of the anthers as the flowering period progressed. Observations on flower-visiting insects indicated that the main flower-visiting insects of yellow-flowered populations were Amegilla leptocoma and A. yunnanensis, while the main flower-visiting insect of the purple-flowered populations was A. leptocoma. Thus, yellow-flowered individuals have a more generalized assemblage of flower visitors, whereas purple-flowered individuals exhibit a more specialized pollination system. This study investigated the differentiation pattern of floral traits among different geographical populations, floral color diversity and pollination adaptation process of P. heterotricha, and provides a scientific basis for biodiversity conservation and pollination service function assessment in National Park of Hainan Tropical Rainforest..
, Available online , doi: 10.15886/j.cnki.rdswxb.20250067
Abstract:
Sequencing batch reactor (SBR) was used to treat domestic wastewater, and the start-up conditions of slightly bulking activated sludge process was investigated under the combined effects of low dissolved oxygen (DO) and low sludge loading (Ns). Moreover, different influent COD/NH4+-N(C/N) ratios (C/N) were designed to examine their impact on nitrogen and phosphorus removal efficiency. The results indicated that under conditions of DO ranging from 0.5 to 0.8 mg·L−1 and Ns between 215.4～258.48 mg COD/L·d, the slightly bulking activated sludge process could be successfully initiated after a brief period of severe bulking, with the sludge volume index (SVI) stabilizing at about 262 mL·g−1. As the influent C/N ratio increased, the number and size of mycelial clumps within the system gradually increased, leading to significant changes in the microbial community, with the abundance of Thiothrix increasing from 30.21% to 54.41%. The influent C/N ratio had a minor impact on COD and NH4+-N removal efficiencies, with COD removal rates reaching 97.21% and NH4+-N removal rates exceeding 98%. But it significantly affected TN and TP removal efficiencies. When the influent C/N ratios were 4, 6, and 11, the average TN removal efficiencies of the system were 37.15%, 52.65%, and 77.94%, respectively, and the average TP removal efficiencies were 36.82%, 45.71%, and 64.30%.
Sequencing batch reactor (SBR) was used to treat domestic wastewater, and the start-up conditions of slightly bulking activated sludge process was investigated under the combined effects of low dissolved oxygen (DO) and low sludge loading (Ns). Moreover, different influent COD/NH4+-N(C/N) ratios (C/N) were designed to examine their impact on nitrogen and phosphorus removal efficiency. The results indicated that under conditions of DO ranging from 0.5 to 0.8 mg·L−1 and Ns between 215.4～258.48 mg COD/L·d, the slightly bulking activated sludge process could be successfully initiated after a brief period of severe bulking, with the sludge volume index (SVI) stabilizing at about 262 mL·g−1. As the influent C/N ratio increased, the number and size of mycelial clumps within the system gradually increased, leading to significant changes in the microbial community, with the abundance of Thiothrix increasing from 30.21% to 54.41%. The influent C/N ratio had a minor impact on COD and NH4+-N removal efficiencies, with COD removal rates reaching 97.21% and NH4+-N removal rates exceeding 98%. But it significantly affected TN and TP removal efficiencies. When the influent C/N ratios were 4, 6, and 11, the average TN removal efficiencies of the system were 37.15%, 52.65%, and 77.94%, respectively, and the average TP removal efficiencies were 36.82%, 45.71%, and 64.30%.
, Available online , doi: 10.15886/j.cnki.rdswxb.202500077
Abstract:
This study investigated the diversity and phenology of Saturniidae moths in Limushan, Hainan Tropical Rainforest National Park. Through light trapping and transect surveys from 2020 to 2024, we recorded 15 Saturniidae species comprising 2 subfamilies and 9 genera, including 3 Hainan-endemic species. Analysis of flight periods revealed peak activity between November and the following August, with 56.34% of moths showing peak phototactic response at 03:00–06:00. Laboratory rearing indicated 6 univoltine species, 2 bivoltine species, and 7 multivoltine species. The faunal composition of Saturniidae in Limushan aligned with tropical zoogeographic regions, reflecting the area's distinctive ecological environment and biodiversity. These findings offer critical data for future species conservation and resource utilization .
This study investigated the diversity and phenology of Saturniidae moths in Limushan, Hainan Tropical Rainforest National Park. Through light trapping and transect surveys from 2020 to 2024, we recorded 15 Saturniidae species comprising 2 subfamilies and 9 genera, including 3 Hainan-endemic species. Analysis of flight periods revealed peak activity between November and the following August, with 56.34% of moths showing peak phototactic response at 03:00–06:00. Laboratory rearing indicated 6 univoltine species, 2 bivoltine species, and 7 multivoltine species. The faunal composition of Saturniidae in Limushan aligned with tropical zoogeographic regions, reflecting the area's distinctive ecological environment and biodiversity. These findings offer critical data for future species conservation and resource utilization .
Antioxidant activity and LC-MS component analysis of water extracts from the flowers of Bombax ceiba
, Available online , doi: 10.15886/j.cnki.rdswxb.20250100
Abstract:
To explore the potential of the aqueous extract of Bombax ceiba flower (BWE) in the field of antioxidation, the effects of different solid-liquid ratios and decoction times on the total flavonoid content and in vitro antioxidant indices such as DPPH radical scavenging rate, ABTS cation radical scavenging rate, and Fe3+ reducing ability of the extract were investigated. The optimal process parameters of solid-liquid ratio and decoction time were screened out. Subsequently, the chemical components of BWE were identified and analyzed by using liquid chromatography-mass spectrometry (LC-MS) to provide key scientific basis for the development and application of BWE in the field of antioxidation. The results showed that within a certain range, the scavenging ability of BWE on DPPH and ABTS radicals and the reducing ability of Fe3+ increased with the concentration. The optimal parameters were a solid-liquid ratio of 1∶12.5 and a decoction time of 1.5 h, with IC50 values being 87.44 mg·L−1 and 46.77 mg·L−1, respectively. However, the Fe3+ reducing ability of BWE was higher than that of vitamin C. These results indicated that BWE had high antioxidant activity. A total of 73 flavonoids were identified in BWE, among which four chemical components in BWE, i.e. quercetin 3-O-glucuronide (49.99%), Delphinidin-3-O-(6''-O-alpha-rhamnopyranosyl-beta-glucopyranoside) (8.24%), rutin (7.45%), and gallocatechin (5.65%), were the highest in relative content and are important pharmacologically active substances. This provides a theoretical basis for further in-depth exploration and utilization of the medicinal potential of flavonoids in BWE.
To explore the potential of the aqueous extract of Bombax ceiba flower (BWE) in the field of antioxidation, the effects of different solid-liquid ratios and decoction times on the total flavonoid content and in vitro antioxidant indices such as DPPH radical scavenging rate, ABTS cation radical scavenging rate, and Fe3+ reducing ability of the extract were investigated. The optimal process parameters of solid-liquid ratio and decoction time were screened out. Subsequently, the chemical components of BWE were identified and analyzed by using liquid chromatography-mass spectrometry (LC-MS) to provide key scientific basis for the development and application of BWE in the field of antioxidation. The results showed that within a certain range, the scavenging ability of BWE on DPPH and ABTS radicals and the reducing ability of Fe3+ increased with the concentration. The optimal parameters were a solid-liquid ratio of 1∶12.5 and a decoction time of 1.5 h, with IC50 values being 87.44 mg·L−1 and 46.77 mg·L−1, respectively. However, the Fe3+ reducing ability of BWE was higher than that of vitamin C. These results indicated that BWE had high antioxidant activity. A total of 73 flavonoids were identified in BWE, among which four chemical components in BWE, i.e. quercetin 3-O-glucuronide (49.99%), Delphinidin-3-O-(6''-O-alpha-rhamnopyranosyl-beta-glucopyranoside) (8.24%), rutin (7.45%), and gallocatechin (5.65%), were the highest in relative content and are important pharmacologically active substances. This provides a theoretical basis for further in-depth exploration and utilization of the medicinal potential of flavonoids in BWE.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250113
Abstract:
Sweet potato (Ipomoea batatas) is an important staple and economic crop in China, but its yield and quality are severely constrained by root-knot nematode (Meloidogyne spp.) infections. To identify effective and safe control agents, a field trial was conducted in Changjiang County, Hainan Province, to evaluate the efficacy of 30% fosthiazate emulsion and 450 g·L−1 cyclobutrifluram suspension concentrate against root-knot nematodes in sweet potato. The results showed that both nematicides significantly (P < 0.05) reduced the disease severity index and tuber infection rate. Among them, cyclobutrifluram showed significantly higher and more persistent efficacy, with relative efficacies of 93.82% and 88.08% at 58 and 77 days after planting, respectively—significantly greater than those of fosthiazate (80.58% and 71.30%). Microscopic observation further revealed that cyclobutrifluram reduced nematode population density in the treated roots by more than 96%, markedly outperforming fosthiazate (47.1%). These findings indicate that cyclobutrifluram possesses strong potential for field application and represents a promising alternative nematicide, particularly under the current scenario of increasing nematode resistance.
Sweet potato (Ipomoea batatas) is an important staple and economic crop in China, but its yield and quality are severely constrained by root-knot nematode (Meloidogyne spp.) infections. To identify effective and safe control agents, a field trial was conducted in Changjiang County, Hainan Province, to evaluate the efficacy of 30% fosthiazate emulsion and 450 g·L−1 cyclobutrifluram suspension concentrate against root-knot nematodes in sweet potato. The results showed that both nematicides significantly (P < 0.05) reduced the disease severity index and tuber infection rate. Among them, cyclobutrifluram showed significantly higher and more persistent efficacy, with relative efficacies of 93.82% and 88.08% at 58 and 77 days after planting, respectively—significantly greater than those of fosthiazate (80.58% and 71.30%). Microscopic observation further revealed that cyclobutrifluram reduced nematode population density in the treated roots by more than 96%, markedly outperforming fosthiazate (47.1%). These findings indicate that cyclobutrifluram possesses strong potential for field application and represents a promising alternative nematicide, particularly under the current scenario of increasing nematode resistance.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250083
Abstract:
Oil-tea Camellia is a perennial woody oil tree species, and reasonable fertilizer application is of great significance for increasing the oil-tea yield. Compound fertilizer at a reasonable ratio of nitrogen (N), phosphorus (P) and potassium (K) can provide balanced nutrition, which is crucial for improving the growth and yield of oil-tea Camellia. In order to develop a specialized compound fertilizer suitable for the soil conditions of oil-tea Camellia plantations in tropical areas, compound fertilizers at four authoritative ratios of nitrogen, phosphorus and potassium were applied to a plantation of oil-tea Camellia. vietnamensis ‘Houchen 3’, and the leaf nutrient contents, the soil physical and chemical properties, and the enzyme activities in the oil-tea plantation were determined. The results showed that the application of compound fertilizers at different NPK ratios can significantly improve the nutritional elements of oil-tea Camellia leaves, as well as the soil physical and chemical properties and soil enzyme activity in the oil-tea plantation. In terms of improving leaf nutrients, soil nutrients and biological activity, the C (N∶P∶K = 1∶2∶2) and D (N∶P∶K = 10∶6∶8) treatments largely have the best effect on oil-tea Camellia in the tropical regions, and had the greatest promoting effect especially on the content of available potassium in soil, with an increase of 92.68% and 116.17% in the 0-20 cm and 20-40 cm soil layers, respectively, compared to the control without fertilization (CK2), which is of great significance for targeted improvement of soil fertility and available potassium content in Hainan oil-tea Camellia plantations. These results provide data for screening specialized compound fertilizers suitable for oil-tea Camellia in the tropical regions, laying a scientific foundation for further research of specialized compound fertilizers for oil-tea Camellia in the tropical regions.
Oil-tea Camellia is a perennial woody oil tree species, and reasonable fertilizer application is of great significance for increasing the oil-tea yield. Compound fertilizer at a reasonable ratio of nitrogen (N), phosphorus (P) and potassium (K) can provide balanced nutrition, which is crucial for improving the growth and yield of oil-tea Camellia. In order to develop a specialized compound fertilizer suitable for the soil conditions of oil-tea Camellia plantations in tropical areas, compound fertilizers at four authoritative ratios of nitrogen, phosphorus and potassium were applied to a plantation of oil-tea Camellia. vietnamensis ‘Houchen 3’, and the leaf nutrient contents, the soil physical and chemical properties, and the enzyme activities in the oil-tea plantation were determined. The results showed that the application of compound fertilizers at different NPK ratios can significantly improve the nutritional elements of oil-tea Camellia leaves, as well as the soil physical and chemical properties and soil enzyme activity in the oil-tea plantation. In terms of improving leaf nutrients, soil nutrients and biological activity, the C (N∶P∶K = 1∶2∶2) and D (N∶P∶K = 10∶6∶8) treatments largely have the best effect on oil-tea Camellia in the tropical regions, and had the greatest promoting effect especially on the content of available potassium in soil, with an increase of 92.68% and 116.17% in the 0-20 cm and 20-40 cm soil layers, respectively, compared to the control without fertilization (CK2), which is of great significance for targeted improvement of soil fertility and available potassium content in Hainan oil-tea Camellia plantations. These results provide data for screening specialized compound fertilizers suitable for oil-tea Camellia in the tropical regions, laying a scientific foundation for further research of specialized compound fertilizers for oil-tea Camellia in the tropical regions.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250072
Abstract:
Biston suppressaria is a pest threatening eucalyptus plantations. Bacteria from its intestine were isolated and identified by using traditional culture and molecular biology methods to find pathogenic strains for biocontrol of this pest. Fifteen bacteria with distinct morphological traits were isolated from naturally infected, lethal B. suppressarias and identified into ten genera, including Enterobacter, Serratia, and Bacillus via 16S rDNA sequence analysis. The Bacillaceae strain B6 showed the highest pathogenicity. After treated with B6 by using the feeding method, B. suppressarias had a cumulative corrected mortality of 60.0% at day 7, with specific pathogenic characteristics observed in the dead insects. Re-isolation yielded colonies morphologically consistent with B6, with a sequence similarity of 99.80%. Moreover, B6 exhibited certain pathogenicity to the other two types of Geometridae larvae, including the common cutworm and small emerald moth, and showed significant insecticidal effects on B. suppressarias at the early infection stage. This study provides a theoretical basis for the use of the Bacillaceae strain B6 to control B. suppressarias and develop eco-friendly control strategies.
Biston suppressaria is a pest threatening eucalyptus plantations. Bacteria from its intestine were isolated and identified by using traditional culture and molecular biology methods to find pathogenic strains for biocontrol of this pest. Fifteen bacteria with distinct morphological traits were isolated from naturally infected, lethal B. suppressarias and identified into ten genera, including Enterobacter, Serratia, and Bacillus via 16S rDNA sequence analysis. The Bacillaceae strain B6 showed the highest pathogenicity. After treated with B6 by using the feeding method, B. suppressarias had a cumulative corrected mortality of 60.0% at day 7, with specific pathogenic characteristics observed in the dead insects. Re-isolation yielded colonies morphologically consistent with B6, with a sequence similarity of 99.80%. Moreover, B6 exhibited certain pathogenicity to the other two types of Geometridae larvae, including the common cutworm and small emerald moth, and showed significant insecticidal effects on B. suppressarias at the early infection stage. This study provides a theoretical basis for the use of the Bacillaceae strain B6 to control B. suppressarias and develop eco-friendly control strategies.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250041
Abstract:
The insect olfactory system plays a pivotal role in host recognition, mating, and oviposition, with odorant-binding proteins (OBPs) serving critical functions in olfactory signal transduction. An attempt was made to analyze the sequence characteristics and ligand-binding properties of two OBPs (TrufOBP1 and TrufOBP5) in Tirathaba rufivena, a major pest infesting inflorescences and fruit of arecanut (Areca catechu), and to validate their behavioral regulatory roles through wind tunnel experiments. Sequence analysis revealed that TrufOBP1 contains four conserved cysteines and is classified as a Minus-C OBP, whereas TrufOBP5 possesses ten conserved cysteines and is categorized as a Plus-C OBP. Fluorescence competitive binding assays demonstrated that TrufOBP1 exhibited broad ligand specificity and was bound to 11 compounds, including nine volatiles from areca nut (Areca catechu) inflorescences and two sex pheromones. In contrast, TrufOBP5 displayed high binding specificity, and interacted exclusively with octyl p-methoxycinnamate and vanillin, suggesting its potential role in host plant recognition. Wind tunnel experiments identified β-myrcene, octyl p-methoxycinnamate, and 3-hexenyl acetate as attractants for female adults from host volatiles. Among sex pheromone components, 2,2,6-trimethyl-6-vinyltetrahydro-2H-pyran-3-OL exhibited the highest attraction activity, while vanillin also demonstrated significant attractant effects. This study elucidates the functional roles of TrufOBP1 and TrufOBP5 in host recognition and sex pheromone perception in T. rufivena, providing a theoretical foundation for pest behavior regulation and supporting the development of olfaction-based green control strategies.
The insect olfactory system plays a pivotal role in host recognition, mating, and oviposition, with odorant-binding proteins (OBPs) serving critical functions in olfactory signal transduction. An attempt was made to analyze the sequence characteristics and ligand-binding properties of two OBPs (TrufOBP1 and TrufOBP5) in Tirathaba rufivena, a major pest infesting inflorescences and fruit of arecanut (Areca catechu), and to validate their behavioral regulatory roles through wind tunnel experiments. Sequence analysis revealed that TrufOBP1 contains four conserved cysteines and is classified as a Minus-C OBP, whereas TrufOBP5 possesses ten conserved cysteines and is categorized as a Plus-C OBP. Fluorescence competitive binding assays demonstrated that TrufOBP1 exhibited broad ligand specificity and was bound to 11 compounds, including nine volatiles from areca nut (Areca catechu) inflorescences and two sex pheromones. In contrast, TrufOBP5 displayed high binding specificity, and interacted exclusively with octyl p-methoxycinnamate and vanillin, suggesting its potential role in host plant recognition. Wind tunnel experiments identified β-myrcene, octyl p-methoxycinnamate, and 3-hexenyl acetate as attractants for female adults from host volatiles. Among sex pheromone components, 2,2,6-trimethyl-6-vinyltetrahydro-2H-pyran-3-OL exhibited the highest attraction activity, while vanillin also demonstrated significant attractant effects. This study elucidates the functional roles of TrufOBP1 and TrufOBP5 in host recognition and sex pheromone perception in T. rufivena, providing a theoretical foundation for pest behavior regulation and supporting the development of olfaction-based green control strategies.
, Available online , doi: 10.15886/j.cnki.rdswxb.20240204
Abstract:
The inorganic salt medium with atrazine as the sole carbon and nitrogen source was used to screen a Trichoderma strain with the best growth performance among 10 Trichoderma strains, and the Trichoderma strain HN154 was selected. The degradation test of atrazine demonstrated that this strain had a high ability to degrade atrazine. Strain HN154 was identified as Trichoderma lentiforme based on morphological characteristics and ITS/RPB2 gene sequence analysis. Optimization of atrazine degradation conditions showed that the atrazine degradation rate was as high as 87.79% when cultured in inorganic salt medium with 10 g/L glucose, pH 7, 25 ℃, and 1% inoculum. Response surface optimization further improved degradation to 89.02% under the conditions of 5.24 g/L glucose, pH 7.78, 0.77% inoculum, and 25.5 ℃. This study provides microbial resources and technical support for efficient atrazine degradation, offering insights into degradation of microbial pesticide residues.
The inorganic salt medium with atrazine as the sole carbon and nitrogen source was used to screen a Trichoderma strain with the best growth performance among 10 Trichoderma strains, and the Trichoderma strain HN154 was selected. The degradation test of atrazine demonstrated that this strain had a high ability to degrade atrazine. Strain HN154 was identified as Trichoderma lentiforme based on morphological characteristics and ITS/RPB2 gene sequence analysis. Optimization of atrazine degradation conditions showed that the atrazine degradation rate was as high as 87.79% when cultured in inorganic salt medium with 10 g/L glucose, pH 7, 25 ℃, and 1% inoculum. Response surface optimization further improved degradation to 89.02% under the conditions of 5.24 g/L glucose, pH 7.78, 0.77% inoculum, and 25.5 ℃. This study provides microbial resources and technical support for efficient atrazine degradation, offering insights into degradation of microbial pesticide residues.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250004
Abstract:
For early and rapid detection of Xanthomonas. oryzae pv. oryzicola (Xoc), the cause of bacterial leaf streak in rice, specific recombinase polymerase amplification (RPA) primers, and Exo fluorescent and nfo probes were designed and screened, respectively, according to the publicly available gene sequence XOCgx_RS03090 of Xoc genome sequence in GenBank. The recombinant plasmid containing Xoc gene was constructed as a positive control. One fluorescent Exo-RPA assay and the other lateral flow strip detection (LFD-RPA) assay were established for rapid detection of Xoc. The sensitivity and specificity of the RPA methods were assessed, and the amplification effects of the RPA methods were explored by optimizing the reaction temperature and reaction time. The results showed that the sensitivity of both the Exo-RPA and LFD-RPA assays for the detection of Xoc were both 4.96 copies·μL−1, and that the optimal temperatures for the reaction were both 39 ℃. Xoc was detected within 12 min by the optimized Exo-RPA method, and within 5 min by the optimized LFD-RPA. The Exo-RPA and LFD-RPA assays developed were highly specific and could specifically and uniquely identify Xoc among plant pathogens in test. Both the RPA methods are highly sensitive, specific and fast, which pose a high potential to the monitoring of the target pathogen and early diagnosis of the disease.
For early and rapid detection of Xanthomonas. oryzae pv. oryzicola (Xoc), the cause of bacterial leaf streak in rice, specific recombinase polymerase amplification (RPA) primers, and Exo fluorescent and nfo probes were designed and screened, respectively, according to the publicly available gene sequence XOCgx_RS03090 of Xoc genome sequence in GenBank. The recombinant plasmid containing Xoc gene was constructed as a positive control. One fluorescent Exo-RPA assay and the other lateral flow strip detection (LFD-RPA) assay were established for rapid detection of Xoc. The sensitivity and specificity of the RPA methods were assessed, and the amplification effects of the RPA methods were explored by optimizing the reaction temperature and reaction time. The results showed that the sensitivity of both the Exo-RPA and LFD-RPA assays for the detection of Xoc were both 4.96 copies·μL−1, and that the optimal temperatures for the reaction were both 39 ℃. Xoc was detected within 12 min by the optimized Exo-RPA method, and within 5 min by the optimized LFD-RPA. The Exo-RPA and LFD-RPA assays developed were highly specific and could specifically and uniquely identify Xoc among plant pathogens in test. Both the RPA methods are highly sensitive, specific and fast, which pose a high potential to the monitoring of the target pathogen and early diagnosis of the disease.
, Available online , doi: 10.15886/j.cnki.rdswxb.20250029
Abstract:
To further understand the role of the TkBZR family genes in the growth, development, and stress resistance of rubber dandelion (Taraxacum kok-saghyz), the TkBZR/BES family genes were identified and analyzed by examining the whole genome sequencing data of T. kok-saghyz. A total of 6 members of the TkBZR family were identified, named from TkBZR1 to TkBZR6, and their distribution on chromosomes, domain characteristics, tissue expression profiles and the spatial-temporal gene expression were analyzed. The results revealed that TkBZR/BES gene family members were distributed on 6 independent scaffolds. Phylogenetic trees indicate that TkBZR/BES gene family members are evolutionarily conserved, with rubber dandelion and lettuce genes clustering in the same subgroup, suggesting a possible close evolutionary relationship between the two. Gene structure and conserved domain analysis show that except for TkBZR5, all the other TkBZR genes contain two exons and one intron, and that all the family members exhibit a highly conserved BES1_N domain. Expression pattern analysis reveals that 5 members are expressed in all five tissues, while one member is almost unexpressed. Additionally, the TkBZR2 gene with the highest expression abundance was successfully cloned, encoding 307 amino acids. Homologous recombination of TkBZR2 was made into prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21(DE3) .The recombinant protein TkBZR2 was expressed in E. coli BL21 (DE3).
To further understand the role of the TkBZR family genes in the growth, development, and stress resistance of rubber dandelion (Taraxacum kok-saghyz), the TkBZR/BES family genes were identified and analyzed by examining the whole genome sequencing data of T. kok-saghyz. A total of 6 members of the TkBZR family were identified, named from TkBZR1 to TkBZR6, and their distribution on chromosomes, domain characteristics, tissue expression profiles and the spatial-temporal gene expression were analyzed. The results revealed that TkBZR/BES gene family members were distributed on 6 independent scaffolds. Phylogenetic trees indicate that TkBZR/BES gene family members are evolutionarily conserved, with rubber dandelion and lettuce genes clustering in the same subgroup, suggesting a possible close evolutionary relationship between the two. Gene structure and conserved domain analysis show that except for TkBZR5, all the other TkBZR genes contain two exons and one intron, and that all the family members exhibit a highly conserved BES1_N domain. Expression pattern analysis reveals that 5 members are expressed in all five tissues, while one member is almost unexpressed. Additionally, the TkBZR2 gene with the highest expression abundance was successfully cloned, encoding 307 amino acids. Homologous recombination of TkBZR2 was made into prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21(DE3) .The recombinant protein TkBZR2 was expressed in E. coli BL21 (DE3).
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