2016 Vol. 7, No. 3
2016, 7(3): 285-289.
doi: 10.15886/j.cnki.rdswxb.2016.03.001
Abstract
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Abstract:
The full length c DNA of PKR gene was cloned from the liver of grouper,Epinephelus coioides,which is 2 151 bp,including 1 863 bp ORF area,encoding 621 amino acids. Its protein molecular weight is 70 157.15 Daltons and PI is 5. 72. BLAST comparison on the NCBI web site found that the PKR of the grouper was highly homologous to that of other species. By constructing prokaryotic expression vector PKR-p ET-32 a,the fusion protein of PKR was obtained and purified. The mouse antiserum of PKR was also prepared. Real-time PCR analysis indicates that the PKR gene is expressed in all tissues of the grouper,most highly in the intestine and then in the head kidney. Subcellular localization of PKR showed that PKR was distributed in both nucleus and cytoplasm,but mainly in the cytoplasm.
The full length c DNA of PKR gene was cloned from the liver of grouper,Epinephelus coioides,which is 2 151 bp,including 1 863 bp ORF area,encoding 621 amino acids. Its protein molecular weight is 70 157.15 Daltons and PI is 5. 72. BLAST comparison on the NCBI web site found that the PKR of the grouper was highly homologous to that of other species. By constructing prokaryotic expression vector PKR-p ET-32 a,the fusion protein of PKR was obtained and purified. The mouse antiserum of PKR was also prepared. Real-time PCR analysis indicates that the PKR gene is expressed in all tissues of the grouper,most highly in the intestine and then in the head kidney. Subcellular localization of PKR showed that PKR was distributed in both nucleus and cytoplasm,but mainly in the cytoplasm.
2016, 7(3): 290-295.
doi: 10.15886/j.cnki.rdswxb.2016.03.002
Abstract:
Thickness of shell inner nacreous layer was measured by using optical coherence tomography in 341 pearl oysters. Correlativity between five microsatellite markers and thickness of shell inner nacreous layer was calculated by one-way analysis of variance. Four out of the five markers（ 2PM206,4PM34,4PM50 and PM6）were correlated with the test trait（ P ＜ 0. 05）. It is shown that oysters with 173 /173 genotype in locus 2PM206,with 189 /189 genotype in locus 4PM34,with genotype 201 /205 in locus 4PM50 and 260 /266 genotype in locus PM6 had significantly higher thickness of shell nacreous layer than other genotype individuals（P ＜ 0. 05）. Individuals with allele 209 had lower values of the tested traits than all the other individuals with alleles in 4PM50 marker,and hence allele 209 may have negative correlation with the thickness of shell nacreous layer（P ＜ 0. 05）.
Thickness of shell inner nacreous layer was measured by using optical coherence tomography in 341 pearl oysters. Correlativity between five microsatellite markers and thickness of shell inner nacreous layer was calculated by one-way analysis of variance. Four out of the five markers（ 2PM206,4PM34,4PM50 and PM6）were correlated with the test trait（ P ＜ 0. 05）. It is shown that oysters with 173 /173 genotype in locus 2PM206,with 189 /189 genotype in locus 4PM34,with genotype 201 /205 in locus 4PM50 and 260 /266 genotype in locus PM6 had significantly higher thickness of shell nacreous layer than other genotype individuals（P ＜ 0. 05）. Individuals with allele 209 had lower values of the tested traits than all the other individuals with alleles in 4PM50 marker,and hence allele 209 may have negative correlation with the thickness of shell nacreous layer（P ＜ 0. 05）.
2016, 7(3): 296-300.
doi: 10.15886/j.cnki.rdswxb.2016.03.003
Abstract:
The ammonia nitrogen,nitrite nitrogen,nitrate nitrogen,inorganic phosphorus,and microorganisms（Vibrio,total heterotrophic bacteria） in farming area and non-farming area in the period from December 2013 to October 2014 were determined to observe the annual changes in water quality and microorganism in tidal flats farming areas where Babylonia areolata was farmed. The results showed the concentrations of ammonia nitrogen,nitrite nitrogen and nitrate nitrogen had the peak values during the months from March to May. The peak value of inorganic phosphorus appeared in October. However,higher concentrations of Vibrio were detected from May to October. The water quality and microorganism parameters in the B. areolate farming area fluctuated,and no obvious difference was found in all the parameters,except ammonia nitrogen between the farming and non-farming areas. The results indicated B. areolata farming did not cause an obvious impact on sea water quality factors and microbial communities under the conditions of current culture area（ 18 ha） and culture density 300 shells/sq. m.
The ammonia nitrogen,nitrite nitrogen,nitrate nitrogen,inorganic phosphorus,and microorganisms（Vibrio,total heterotrophic bacteria） in farming area and non-farming area in the period from December 2013 to October 2014 were determined to observe the annual changes in water quality and microorganism in tidal flats farming areas where Babylonia areolata was farmed. The results showed the concentrations of ammonia nitrogen,nitrite nitrogen and nitrate nitrogen had the peak values during the months from March to May. The peak value of inorganic phosphorus appeared in October. However,higher concentrations of Vibrio were detected from May to October. The water quality and microorganism parameters in the B. areolate farming area fluctuated,and no obvious difference was found in all the parameters,except ammonia nitrogen between the farming and non-farming areas. The results indicated B. areolata farming did not cause an obvious impact on sea water quality factors and microbial communities under the conditions of current culture area（ 18 ha） and culture density 300 shells/sq. m.
2016, 7(3): 301-306.
doi: 10.15886/j.cnki.rdswxb.2016.03.004
Abstract:
In order to investigate the infection rates of White Spot Syndrome Virus（ WSSV） among aquatic Litopenaeus vannamei,we tested the samples collected from different regions of Pearl River Delta using Polymerase Chain Reaction（ PCR） method. The results show that WSSV infection prevailed to different extents in different regions and in different culture months in L. vannamei during 2012- 2014 in the major aquatic culture areas of th Pearl River Delta: Zhuhai,Jiangmen and Zhongshan,where L. vannamei had an evidently higher infection rate of WSSV at the larval stage than at the juvenile or adult stages.
In order to investigate the infection rates of White Spot Syndrome Virus（ WSSV） among aquatic Litopenaeus vannamei,we tested the samples collected from different regions of Pearl River Delta using Polymerase Chain Reaction（ PCR） method. The results show that WSSV infection prevailed to different extents in different regions and in different culture months in L. vannamei during 2012- 2014 in the major aquatic culture areas of th Pearl River Delta: Zhuhai,Jiangmen and Zhongshan,where L. vannamei had an evidently higher infection rate of WSSV at the larval stage than at the juvenile or adult stages.
2016, 7(3): 307-313.
doi: 10.15886/j.cnki.rdswxb.2016.03.005
Abstract:
Infectious hypodermal and hematopoietic necrosis virus（ IHHNV） causes large economic losses to the shrimp aquaculture industry in the world. It is necessary to develop a fast,easy and accurate method to detect IHHNV. In this study,we developed a real-time fluorescence loop-mediated isothermal amplification assay for IHHNV detection on a small ESE-Quant Tube Scanner device and evaluated its sensitivity,specificity and rapidity. This method had a detection limit of 106 dilution template of genomic DNA within 30 min under isothermal condition at 63 C,and 24 copies of plasmid were detected. Furthermore,the approach had no non-specific signal response to other DNAs,including those of 27 species of aquatic animals and penaeid shrimp pathogens. This method had a positive detection rate of 59. 87%,similar to that of the real-time PCR but higher than other detection methods,and hence has great application potential in on-the-spot detection of IHHNV in shrimps.
Infectious hypodermal and hematopoietic necrosis virus（ IHHNV） causes large economic losses to the shrimp aquaculture industry in the world. It is necessary to develop a fast,easy and accurate method to detect IHHNV. In this study,we developed a real-time fluorescence loop-mediated isothermal amplification assay for IHHNV detection on a small ESE-Quant Tube Scanner device and evaluated its sensitivity,specificity and rapidity. This method had a detection limit of 106 dilution template of genomic DNA within 30 min under isothermal condition at 63 C,and 24 copies of plasmid were detected. Furthermore,the approach had no non-specific signal response to other DNAs,including those of 27 species of aquatic animals and penaeid shrimp pathogens. This method had a positive detection rate of 59. 87%,similar to that of the real-time PCR but higher than other detection methods,and hence has great application potential in on-the-spot detection of IHHNV in shrimps.
2016, 7(3): 314-317.
doi: 10.15886/j.cnki.rdswxb.2016.03.006
Abstract:
Live fish of black seabream（ Sparus macrocephlus） were stored without water at respective temperatures of -2 ℃,0 ℃,2 ℃,4 ℃ and 6 ℃,and their survival rate,O2 consumption,CO2 release,serum cortisol and muscle glycogen were determined and compared to choose an optimum temperature for waterless storage of the live fish. The results showed that the live fish of the black seabream stored without water at 0 ℃ had a survival rate of 100% even after 36 h,the least consumption of O2,and the slowest release of CO2,with their serum cortisol content always being closest to the normal value of the live fish. The temperature of 0 ℃ is hence optimal for waterless storage of the live fish of the black seabream.
Live fish of black seabream（ Sparus macrocephlus） were stored without water at respective temperatures of -2 ℃,0 ℃,2 ℃,4 ℃ and 6 ℃,and their survival rate,O2 consumption,CO2 release,serum cortisol and muscle glycogen were determined and compared to choose an optimum temperature for waterless storage of the live fish. The results showed that the live fish of the black seabream stored without water at 0 ℃ had a survival rate of 100% even after 36 h,the least consumption of O2,and the slowest release of CO2,with their serum cortisol content always being closest to the normal value of the live fish. The temperature of 0 ℃ is hence optimal for waterless storage of the live fish of the black seabream.
2016, 7(3): 318-324.
doi: 10.15886/j.cnki.rdswxb.2016.03.007
Abstract:
Hevein,as an important phytolectin,participating in various stress responses of rubber tree,has important biological functions. In situ PCR technique was used to locate the four hevein genes（ Hev1. 1,Hev1. 2,Hev2. 1 and Hev2. 2） of rubber tree. And the 4 genes was detected and validated by in situ hybridization. The results indicated that these four genes were located on the long arm of chromosome 8,the long arm of chromosome 7,the short arm of chromosome 6 and the long arm of chromosome 12,respectively,with their average percentage distances from the centromere to the hybridization site being 10. 88,31. 51,63. 81 and 67. 92,respectively.
Hevein,as an important phytolectin,participating in various stress responses of rubber tree,has important biological functions. In situ PCR technique was used to locate the four hevein genes（ Hev1. 1,Hev1. 2,Hev2. 1 and Hev2. 2） of rubber tree. And the 4 genes was detected and validated by in situ hybridization. The results indicated that these four genes were located on the long arm of chromosome 8,the long arm of chromosome 7,the short arm of chromosome 6 and the long arm of chromosome 12,respectively,with their average percentage distances from the centromere to the hybridization site being 10. 88,31. 51,63. 81 and 67. 92,respectively.
2016, 7(3): 325-331.
doi: 10.15886/j.cnki.rdswxb.2016.03.008
Abstract:
In this paper,we observed the agronomic traits of the hybrids between Hainan common wild rice（ Oryza rufipogon Griff.） and bar transgenic rice（ abbreviated as F1hybrids） to analysis their adaptability under different water depth. Results showed that the seed vigor of F1 hybrids generally higher than that of common wild rice. When the water depth reached 10 cm,the biomass of wild rice was obviously better than that of F1 hybrids in general,and the biomass both wild rice and F1 hybrids decreased with water depth increasing. In plant height,wild rice was generally higher than that of F1 hybrids,and the water depth had a little impact on their leaf areas. Comparing the growth of weeds between this experiment and the simulating condition of in situ conservation,we found the deep water had an obviously inhibitory effect on weed growth.
In this paper,we observed the agronomic traits of the hybrids between Hainan common wild rice（ Oryza rufipogon Griff.） and bar transgenic rice（ abbreviated as F1hybrids） to analysis their adaptability under different water depth. Results showed that the seed vigor of F1 hybrids generally higher than that of common wild rice. When the water depth reached 10 cm,the biomass of wild rice was obviously better than that of F1 hybrids in general,and the biomass both wild rice and F1 hybrids decreased with water depth increasing. In plant height,wild rice was generally higher than that of F1 hybrids,and the water depth had a little impact on their leaf areas. Comparing the growth of weeds between this experiment and the simulating condition of in situ conservation,we found the deep water had an obviously inhibitory effect on weed growth.
2016, 7(3): 332-337.
doi: 10.15886/j.cnki.rdswxb.2016.03.009
Abstract:
Fruits of two papaya cultivars,‘Dabai’and ‘Zhongbai’,were collected to determine their changes in fruit quality and pericarp pigment when stored at 20 ℃ with a relative humidity of 80%. With an increase in storage duration these two papaya varieties tended to improve their soluble total sugar,soluble solid and Vc content in fruit. Pericarp color was changed gradually from green to yellow. When the pericarp a value changed from negative to positive,the b and L value was reduced,and the h value was decreased. Papaya Zhongbai turned yellower in pericarp than Papaya Dabai under the same storage conditions. At the storage day 8 the total amount of chlorophyll was higher in Dabai than in Zhongbai. Correlation analysis of pericarp pigments showed the pericarp color of papaya was positively correlated to the content of chlorophyll a,chlorophyll b,carotenoid,total phenols,flavonoid and anthocyanin.
Fruits of two papaya cultivars,‘Dabai’and ‘Zhongbai’,were collected to determine their changes in fruit quality and pericarp pigment when stored at 20 ℃ with a relative humidity of 80%. With an increase in storage duration these two papaya varieties tended to improve their soluble total sugar,soluble solid and Vc content in fruit. Pericarp color was changed gradually from green to yellow. When the pericarp a value changed from negative to positive,the b and L value was reduced,and the h value was decreased. Papaya Zhongbai turned yellower in pericarp than Papaya Dabai under the same storage conditions. At the storage day 8 the total amount of chlorophyll was higher in Dabai than in Zhongbai. Correlation analysis of pericarp pigments showed the pericarp color of papaya was positively correlated to the content of chlorophyll a,chlorophyll b,carotenoid,total phenols,flavonoid and anthocyanin.
2016, 7(3): 338-342.
doi: 10.15886/j.cnki.rdswxb.2016.03.010
Abstract:
Paddy soils were sampled from Qiongzhong county and Dongfang city in Hainan Province,where the humidity was highly different,,to analyze the influences of the dry and wet climatic conditions in tropical region of China on organic matter and its components in the paddy soils. The relationships between the organic matter and its components,and the organic matter sequestration potential were investigated by using the method for physical fractionation. Dry and wet climatic conditions affected the content of soil organic matter and its components. The paddy soils contained higher organic matter and components in the humid climatic region than in the semiarid climatic region. The organic matter content showed a significantly positive linear correlation with the light fraction organic matter（ LFOM） content and the heavy fraction organic matter（ HFOM） content,and the HFOM content was also significantly positively correlated with fulvic acid（ FA）. Paddy soils had higher potential of organic matter fixation in the semiarid region than in the humid region. Humid climate is conducive to the formation of HFOM. The soil humus in the tropical region had weak combining ability and contained mostly FA.Measures should be taken to increase the potential of paddy soils in carbon sequestration in the dry tropical region.
Paddy soils were sampled from Qiongzhong county and Dongfang city in Hainan Province,where the humidity was highly different,,to analyze the influences of the dry and wet climatic conditions in tropical region of China on organic matter and its components in the paddy soils. The relationships between the organic matter and its components,and the organic matter sequestration potential were investigated by using the method for physical fractionation. Dry and wet climatic conditions affected the content of soil organic matter and its components. The paddy soils contained higher organic matter and components in the humid climatic region than in the semiarid climatic region. The organic matter content showed a significantly positive linear correlation with the light fraction organic matter（ LFOM） content and the heavy fraction organic matter（ HFOM） content,and the HFOM content was also significantly positively correlated with fulvic acid（ FA）. Paddy soils had higher potential of organic matter fixation in the semiarid region than in the humid region. Humid climate is conducive to the formation of HFOM. The soil humus in the tropical region had weak combining ability and contained mostly FA.Measures should be taken to increase the potential of paddy soils in carbon sequestration in the dry tropical region.
2016, 7(3): 343-347.
doi: 10.15886/j.cnki.rdswxb.2016.03.011
Abstract:
Endophytic actinomycete strain DJ15 was isolated from Lobelia clavata,a medicinal plant,and used to antagonize fusarium wilt pathogen. The carbon source test,nitrogen source test,different inorganic salts single-factor test,orthogonal test and main fermentation conditions test were carried to optimize the cultural medium and conditions for this strain. The results showed that the optimum medium formula for the strain DJ15 were glucose 1. 5%,soybean powder 1%,peptone 3%,NH4Cl1.0%,NaClo.4%,CaCO30.3%,MgSO40. 1% and K2SO40. 1%. Optimal culture conditions were p H6,15% inoculation quantity,rotation speed 100r·min-1 and culture time 120 h. After optimization,the inhibitory rate of the strain DJ15 on the banana fusarium wilt was 73. 58%,which was 135. 11% higher than that reported earlier.
Endophytic actinomycete strain DJ15 was isolated from Lobelia clavata,a medicinal plant,and used to antagonize fusarium wilt pathogen. The carbon source test,nitrogen source test,different inorganic salts single-factor test,orthogonal test and main fermentation conditions test were carried to optimize the cultural medium and conditions for this strain. The results showed that the optimum medium formula for the strain DJ15 were glucose 1. 5%,soybean powder 1%,peptone 3%,NH4Cl1.0%,NaClo.4%,CaCO30.3%,MgSO40. 1% and K2SO40. 1%. Optimal culture conditions were p H6,15% inoculation quantity,rotation speed 100r·min-1 and culture time 120 h. After optimization,the inhibitory rate of the strain DJ15 on the banana fusarium wilt was 73. 58%,which was 135. 11% higher than that reported earlier.
2016, 7(3): 348-352.
doi: 10.15886/j.cnki.rdswxb.2016.03.012
Abstract:
Essential oil of Lavender（ Lavendula angustifolia Mill） was used to induce the growth of Vicia faba root tip cells to test its genotoxicity,and the correlation between the micronucleus frequency and lipid peroxidation of the root tip cells induced were investigated. The result indicated that lavender essential oil could inhibit the growth of V. faba root tip cells at the concentration of 1. 000 0 mg·L-1,but it had no obvious genetic toxicity at the concentration of 0-0. 200 0 mg·L-1,and showed increasing resistance to mutation and oxidation.Induced with the lavender essential oil at the concentrations of 0-0. 200 0 mg·L-1the root tip cells showed positive correlation between their micronuclei frequency and their lipid peroxidation,and the correlation coefficient（ R2） was 0. 83. When induced with the lavender essential oil at a concentration of higher than 0. 200 0 mg·L-1,V. faba root tip cells produced obvious biological damage,and their normal physiological metabolism and stress response were affected.
Essential oil of Lavender（ Lavendula angustifolia Mill） was used to induce the growth of Vicia faba root tip cells to test its genotoxicity,and the correlation between the micronucleus frequency and lipid peroxidation of the root tip cells induced were investigated. The result indicated that lavender essential oil could inhibit the growth of V. faba root tip cells at the concentration of 1. 000 0 mg·L-1,but it had no obvious genetic toxicity at the concentration of 0-0. 200 0 mg·L-1,and showed increasing resistance to mutation and oxidation.Induced with the lavender essential oil at the concentrations of 0-0. 200 0 mg·L-1the root tip cells showed positive correlation between their micronuclei frequency and their lipid peroxidation,and the correlation coefficient（ R2） was 0. 83. When induced with the lavender essential oil at a concentration of higher than 0. 200 0 mg·L-1,V. faba root tip cells produced obvious biological damage,and their normal physiological metabolism and stress response were affected.
2016, 7(3): 353-357.
doi: 10.15886/j.cnki.rdswxb.2016.03.013
Abstract:
The individuals of 8 clones Qionghaiyou of Camellia vietnamensis T. C. Huang ex Hu were observed in terms of botanical traits,yield and oil physicochemical properties. Observations showed that these 8 camellia clones Qionghaiyou had different genetic basis and were morphologically different: dwarf,semi-dwarf or normal in trees. They yielded high and stable,especially Clones Qionghaiyou 1,3,7 and 8. According to the National Standard GB 11765-2003 camellia oil,the seed oil of all the camellia clones Qionghaiyou fell within the standard range of acid value and peroxide value with a bit higher saponification value and a bit lower iodine values. The acid value and peroxide value were much lower than those in the National Standard,indicating these camellia seed oils had high storage duration and anti-oxidation.
The individuals of 8 clones Qionghaiyou of Camellia vietnamensis T. C. Huang ex Hu were observed in terms of botanical traits,yield and oil physicochemical properties. Observations showed that these 8 camellia clones Qionghaiyou had different genetic basis and were morphologically different: dwarf,semi-dwarf or normal in trees. They yielded high and stable,especially Clones Qionghaiyou 1,3,7 and 8. According to the National Standard GB 11765-2003 camellia oil,the seed oil of all the camellia clones Qionghaiyou fell within the standard range of acid value and peroxide value with a bit higher saponification value and a bit lower iodine values. The acid value and peroxide value were much lower than those in the National Standard,indicating these camellia seed oils had high storage duration and anti-oxidation.
2016, 7(3): 358-362.
doi: 10.15886/j.cnki.rdswxb.2016.03.014
Abstract:
Pogostemon cablin seedlings were watered with 5 allelochemicals（ benzoic acid,vanillic acid,salicylic acid,p-hydroxy benzoic acid and malonic acid） at the concentration of 0. 5,1. 0,5. 0,10. 0 mmol·L-1,respectively to observe their growth,and their activities of superoxide disproportionation enzyme（ SOD）,peroxidase（ POD） and membrane lipid peroxidation products（ MDA）. The results showed that these 5 allelochemicals had obvious effects on the growth of P. cablin seedlings,and tended to promote the plant growth at a low concentration and suppress plant growth at a high concentration. The membrane permeability,MDA content and POD activities were obviously increased with the concentration of the allelochemicals,but the SOD activities were first increased at a lower concentration and then decreased at a higher concentration. The growth of P. cablin seedling depended on both allelochemicals and their concentrations. The growth and development of P. cablin was restrained obviously in the treatment of p-hydroxy benzoic acid at a concentration of 10. 0 mmol·L-1.
Pogostemon cablin seedlings were watered with 5 allelochemicals（ benzoic acid,vanillic acid,salicylic acid,p-hydroxy benzoic acid and malonic acid） at the concentration of 0. 5,1. 0,5. 0,10. 0 mmol·L-1,respectively to observe their growth,and their activities of superoxide disproportionation enzyme（ SOD）,peroxidase（ POD） and membrane lipid peroxidation products（ MDA）. The results showed that these 5 allelochemicals had obvious effects on the growth of P. cablin seedlings,and tended to promote the plant growth at a low concentration and suppress plant growth at a high concentration. The membrane permeability,MDA content and POD activities were obviously increased with the concentration of the allelochemicals,but the SOD activities were first increased at a lower concentration and then decreased at a higher concentration. The growth of P. cablin seedling depended on both allelochemicals and their concentrations. The growth and development of P. cablin was restrained obviously in the treatment of p-hydroxy benzoic acid at a concentration of 10. 0 mmol·L-1.
2016, 7(3): 363-367.
doi: 10.15886/j.cnki.rdswxb.2016.03.015
Abstract:
Tissue cultured plants of 4 species of Zingiberaceae flower plants,Curcuma longa L,Curcuma aromatica Salisb,Curcuma wenyujin Y. H. Chen et C. Ling and Alpinia zerumbet cv Variegata were transferred onto 5 culture mediums to observe their plant and root growth. Observations showed that all the tissue cultured plants cultured on different mediums had different survival rates. C. longa L and C. wenyujin Y. H. Chen et C. Ling had relatively higher adaptability to the environment than other ginger species,followed by C. aromatica Salisb,and then A. zerumbet cv Vaniegata. The culture mediums had different effects on the growth of the tissue cultured plants,and the mediums at presence of coconut coir dust or the nutrient soil were found suitable for the growth of the tissue cultured plants and gave higher survival rate and plant height,more leaf and root number,and wider new leaves than the other mediums.
Tissue cultured plants of 4 species of Zingiberaceae flower plants,Curcuma longa L,Curcuma aromatica Salisb,Curcuma wenyujin Y. H. Chen et C. Ling and Alpinia zerumbet cv Variegata were transferred onto 5 culture mediums to observe their plant and root growth. Observations showed that all the tissue cultured plants cultured on different mediums had different survival rates. C. longa L and C. wenyujin Y. H. Chen et C. Ling had relatively higher adaptability to the environment than other ginger species,followed by C. aromatica Salisb,and then A. zerumbet cv Vaniegata. The culture mediums had different effects on the growth of the tissue cultured plants,and the mediums at presence of coconut coir dust or the nutrient soil were found suitable for the growth of the tissue cultured plants and gave higher survival rate and plant height,more leaf and root number,and wider new leaves than the other mediums.
2016, 7(3): 368-372.
doi: 10.15886/j.cnki.rdswxb.2016.03.016
Abstract:
Fengshui woods is a unique green culture heritage of South China and Hong Kong,and has a very high value of ecosystem service function and landscape culture. A survey was made of the medicinal plant resources in Fengshui woods in 66 villages of Zhongshan city,Guangdong Province,China. In these woods there exists a total of 451 species and varieties falling into 324 genera under 131 families of medicinal plants in Zhongshan city. Among them there are 33 species of fern plants belonging to 22 genera under 18 families; 4 species of gymnosperms plants belonging to 3 genera under 3 families; 368 species of dicotyledonous plants belonging to 264 genera under95 families,and 46 species of monocotyledon plants belonging to 35 genera under 15 families. This survey might provide theoretical reference for the development and utilization of medicinal plants in Zhongshan city.
Fengshui woods is a unique green culture heritage of South China and Hong Kong,and has a very high value of ecosystem service function and landscape culture. A survey was made of the medicinal plant resources in Fengshui woods in 66 villages of Zhongshan city,Guangdong Province,China. In these woods there exists a total of 451 species and varieties falling into 324 genera under 131 families of medicinal plants in Zhongshan city. Among them there are 33 species of fern plants belonging to 22 genera under 18 families; 4 species of gymnosperms plants belonging to 3 genera under 3 families; 368 species of dicotyledonous plants belonging to 264 genera under95 families,and 46 species of monocotyledon plants belonging to 35 genera under 15 families. This survey might provide theoretical reference for the development and utilization of medicinal plants in Zhongshan city.
2016, 7(3): 373-375.
doi: 10.15886/j.cnki.rdswxb.2016.03.017
Abstract:
Different parts of aerial roots of Ficus microcarpa L. were collected to determine their content of total phenolic acids and total flavonoids,and the antioxidant activities of the extracts of different root parts were tested by using the DPPH and ABTS methods for assessment of in vitro free radical scavenging activities. Results showed that the top part of the roots contained the highest total phenolic acids and flavonoids,and that the top,middle and bottom parts of the roots contained total phenol of 0. 34 mg·L-1,0. 31 mg·L-1 and 0. 21 mg·L-1,respectively,and total flavonoids of 3. 88 mg·L-1,3. 42 mg·L-1 and 2. 91 mg·L-1,respectively. The top root part contained the highest antioxidant activity,followed by the middle and bottom root parts. Total phenolic acids were highly correlated with antioxidant activity,which indicates that the antioxidant activity might be related to phenolic acids.
Different parts of aerial roots of Ficus microcarpa L. were collected to determine their content of total phenolic acids and total flavonoids,and the antioxidant activities of the extracts of different root parts were tested by using the DPPH and ABTS methods for assessment of in vitro free radical scavenging activities. Results showed that the top part of the roots contained the highest total phenolic acids and flavonoids,and that the top,middle and bottom parts of the roots contained total phenol of 0. 34 mg·L-1,0. 31 mg·L-1 and 0. 21 mg·L-1,respectively,and total flavonoids of 3. 88 mg·L-1,3. 42 mg·L-1 and 2. 91 mg·L-1,respectively. The top root part contained the highest antioxidant activity,followed by the middle and bottom root parts. Total phenolic acids were highly correlated with antioxidant activity,which indicates that the antioxidant activity might be related to phenolic acids.
2016, 7(3): 376-380.
doi: 10.15886/j.cnki.rdswxb.2016.03.018
Abstract:
A ten-year-old plant of agarwood（ Aquilaria sinensis） was induced by liquid transfusion technology,using healthy layer,transition and agarwood layers of the stems transfusion treated for 15 days as materials. Aidlab EASY-spin plus RN38 RNA extraction kits was modified in many key steps to improve the total RNA product qualities which were analyzed and then compared with those of the unmodified RNA extraction method. The results showed that the total RNA extracted by the modified RNA extraction method had better quality,purity and integrity than that of the unmodified method,in which the 28 S and 18 S bands were more legible with no impurity nor degradation,A260/ A280 was approximated to 2. 0,and the RIN greater than 6. This suggests that the modified method for extracting total RNA in different A. sinensis tissues transfusion treated produces high quality total RNA which can be used for transcriptome data analysis.
A ten-year-old plant of agarwood（ Aquilaria sinensis） was induced by liquid transfusion technology,using healthy layer,transition and agarwood layers of the stems transfusion treated for 15 days as materials. Aidlab EASY-spin plus RN38 RNA extraction kits was modified in many key steps to improve the total RNA product qualities which were analyzed and then compared with those of the unmodified RNA extraction method. The results showed that the total RNA extracted by the modified RNA extraction method had better quality,purity and integrity than that of the unmodified method,in which the 28 S and 18 S bands were more legible with no impurity nor degradation,A260/ A280 was approximated to 2. 0,and the RIN greater than 6. This suggests that the modified method for extracting total RNA in different A. sinensis tissues transfusion treated produces high quality total RNA which can be used for transcriptome data analysis.
2016, 7(3): 381-386.
doi: 10.15886/j.cnki.rdswxb.2016.03.019
Abstract:
In order to study the chemical constituents and bioactivity of volatile oil from the leaf of Moringa oleifera Lam,the volatile oil was extracted by water distillation method,and its components were separated and identified using gas chromatography-mass spectrometer（ GC-MS）. The antimicrobial,anti-acetylcholinesterase and cytotoxicity activities of the volatile oil were evaluated by the 2-fold dilution method,the colorimetric method and the MTT method,respectively. Thirty-four compounds were separated,of which thirty-three were identified and their relative content was about 85. 94 % of the total volatile oil content. The main components included palmitic acid（ 38. 63%）,phytol（ 6. 09%）,ethyl palmitate（ 4. 87%）,and heptacosane（ 4. 31%）. Furthermore,the volatile oil exhibited activities against Staphylococcus aureus and acetylcholinesterase.
In order to study the chemical constituents and bioactivity of volatile oil from the leaf of Moringa oleifera Lam,the volatile oil was extracted by water distillation method,and its components were separated and identified using gas chromatography-mass spectrometer（ GC-MS）. The antimicrobial,anti-acetylcholinesterase and cytotoxicity activities of the volatile oil were evaluated by the 2-fold dilution method,the colorimetric method and the MTT method,respectively. Thirty-four compounds were separated,of which thirty-three were identified and their relative content was about 85. 94 % of the total volatile oil content. The main components included palmitic acid（ 38. 63%）,phytol（ 6. 09%）,ethyl palmitate（ 4. 87%）,and heptacosane（ 4. 31%）. Furthermore,the volatile oil exhibited activities against Staphylococcus aureus and acetylcholinesterase.
2016, 7(3): 387-394.
doi: 10.15886/j.cnki.rdswxb.2016.03.020
Abstract:
Pteris fauriei complex includes Pteris fauriei,P. fauriei var. chinensis,P. angustipinnula,P. majestica,P. splendida and so on. The taxonomic cluster of the complex is easily confused due to the morphological similarity of the members of the complex. Morphological observation of the spores of five members in this complex was performed by a scanning electron microscope（ SEM）,which could help to clarify their genetic relationship. It was found that the lophate-like ornamentation is an important feature,The complex could be divided into four groups based on the presence and distribution of the lophate ornamentation. And the spore morphology is species-specific in the complex,except in that P. fauriei and P. fauriei var. chinensis shared similar spore morphology and are hard to distinguish from each other. In addition,the "conjoined spores"phenomenon was first found in P. splendida.
Pteris fauriei complex includes Pteris fauriei,P. fauriei var. chinensis,P. angustipinnula,P. majestica,P. splendida and so on. The taxonomic cluster of the complex is easily confused due to the morphological similarity of the members of the complex. Morphological observation of the spores of five members in this complex was performed by a scanning electron microscope（ SEM）,which could help to clarify their genetic relationship. It was found that the lophate-like ornamentation is an important feature,The complex could be divided into four groups based on the presence and distribution of the lophate ornamentation. And the spore morphology is species-specific in the complex,except in that P. fauriei and P. fauriei var. chinensis shared similar spore morphology and are hard to distinguish from each other. In addition,the "conjoined spores"phenomenon was first found in P. splendida.
Advances in Development of Food Accurate Rapid Detection Technologies for Important Harmful Residues
2016, 7(3): 395-401.
doi: 10.15886/j.cnki.rdswxb.2016.03.021
Abstract:
The food safety issues of public concern have cropped up recently,and resulted in development of food safety rapid detection technologies for important harmful residues in food. These technologies included enzyme inhibition technology,immunology,chemiluminescence immunoassay,time-resolved fluorescence analysis,fluorescence polarization assay technique and new pretreatment methods. All these technologies and their application and development trend in food safety control were reviewed,and the prospects for development of the rapid detection technologies for the food safety control were discussed.
The food safety issues of public concern have cropped up recently,and resulted in development of food safety rapid detection technologies for important harmful residues in food. These technologies included enzyme inhibition technology,immunology,chemiluminescence immunoassay,time-resolved fluorescence analysis,fluorescence polarization assay technique and new pretreatment methods. All these technologies and their application and development trend in food safety control were reviewed,and the prospects for development of the rapid detection technologies for the food safety control were discussed.
2016, 7(3): 402-408.
doi: 10.15886/j.cnki.rdswxb.2016.03.022
Abstract:
This review summarize the research progress of the research strategies on the plant N-glycosylation and the research progress of the plant glycoproteomics,and it will provided the clue and ideal for the better development in the future study on plant glycoprotein.
This review summarize the research progress of the research strategies on the plant N-glycosylation and the research progress of the plant glycoproteomics,and it will provided the clue and ideal for the better development in the future study on plant glycoprotein.
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