Physical Locations of Four Members of Hevein Gene Family in Hevea brasiliensis
doi: 10.15886/j.cnki.rdswxb.2016.03.007
- Received Date: 2015-03-29
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Key words:
- Hevea brasiliensis /
- Hevein(Hev1.1,Hev1.2,Hev2.1,Hev2.2) /
- in situ PCR /
- fluorescence in situ hybridization(FISH)
Abstract: Hevein,as an important phytolectin,participating in various stress responses of rubber tree,has important biological functions. In situ PCR technique was used to locate the four hevein genes( Hev1. 1,Hev1. 2,Hev2. 1 and Hev2. 2) of rubber tree. And the 4 genes was detected and validated by in situ hybridization. The results indicated that these four genes were located on the long arm of chromosome 8,the long arm of chromosome 7,the short arm of chromosome 6 and the long arm of chromosome 12,respectively,with their average percentage distances from the centromere to the hybridization site being 10. 88,31. 51,63. 81 and 67. 92,respectively.
Citation: | PENG Baofeng, WANG Ying, GAO Heqiong, ZHUANG Nansheng. Physical Locations of Four Members of Hevein Gene Family in Hevea brasiliensis[J]. Journal of Tropical Biology, 2016, 7(3): 318-324. doi: 10.15886/j.cnki.rdswxb.2016.03.007 |