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Volume 7 Issue 3
Sep.  2016
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Article Navigation >  Journal of Tropical Biology  > 2016  >  7(3): 343-347

YANG Liling, HUANG Mianjia, ZHANG Xiyan, GAO Zhufeng. Optimization of Fermentation Conditions for Streptomyces Strain DJ15 with Biological Control of Banana Fusarium Wilt[J]. Journal of Tropical Biology, 2016, 7(3): 343-347. doi: 10.15886/j.cnki.rdswxb.2016.03.011
Citation: YANG Liling, HUANG Mianjia, ZHANG Xiyan, GAO Zhufeng. Optimization of Fermentation Conditions for Streptomyces Strain DJ15 with Biological Control of Banana Fusarium Wilt[J]. Journal of Tropical Biology, 2016, 7(3): 343-347. doi: 10.15886/j.cnki.rdswxb.2016.03.011
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Optimization of Fermentation Conditions for Streptomyces Strain DJ15 with Biological Control of Banana Fusarium Wilt

doi: 10.15886/j.cnki.rdswxb.2016.03.011

  • 1. College of Horticulture and Landscape Architecture, Hainan University, Haikou, Hainan 570228;

  • 2. Institute of Tropicol and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China

  • Received Date: 2016-06-12
  • Endophytic actinomycete strain DJ15 was isolated from Lobelia clavata,a medicinal plant,and used to antagonize fusarium wilt pathogen. The carbon source test,nitrogen source test,different inorganic salts single-factor test,orthogonal test and main fermentation conditions test were carried to optimize the cultural medium and conditions for this strain. The results showed that the optimum medium formula for the strain DJ15 were glucose 1. 5%,soybean powder 1%,peptone 3%,NH4Cl1.0%,NaClo.4%,CaCO30.3%,MgSO40. 1% and K2SO40. 1%. Optimal culture conditions were p H6,15% inoculation quantity,rotation speed 100r·min-1 and culture time 120 h. After optimization,the inhibitory rate of the strain DJ15 on the banana fusarium wilt was 73. 58%,which was 135. 11% higher than that reported earlier.
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Optimization of Fermentation Conditions for Streptomyces Strain DJ15 with Biological Control of Banana Fusarium Wilt

doi: 10.15886/j.cnki.rdswxb.2016.03.011
  • 1. College of Horticulture and Landscape Architecture, Hainan University, Haikou, Hainan 570228;
  • 2. Institute of Tropicol and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
  • Received Date: 2016-06-12

Abstract: Endophytic actinomycete strain DJ15 was isolated from Lobelia clavata,a medicinal plant,and used to antagonize fusarium wilt pathogen. The carbon source test,nitrogen source test,different inorganic salts single-factor test,orthogonal test and main fermentation conditions test were carried to optimize the cultural medium and conditions for this strain. The results showed that the optimum medium formula for the strain DJ15 were glucose 1. 5%,soybean powder 1%,peptone 3%,NH4Cl1.0%,NaClo.4%,CaCO30.3%,MgSO40. 1% and K2SO40. 1%. Optimal culture conditions were p H6,15% inoculation quantity,rotation speed 100r·min-1 and culture time 120 h. After optimization,the inhibitory rate of the strain DJ15 on the banana fusarium wilt was 73. 58%,which was 135. 11% higher than that reported earlier.

YANG Liling, HUANG Mianjia, ZHANG Xiyan, GAO Zhufeng. Optimization of Fermentation Conditions for Streptomyces Strain DJ15 with Biological Control of Banana Fusarium Wilt[J]. Journal of Tropical Biology, 2016, 7(3): 343-347. doi: 10.15886/j.cnki.rdswxb.2016.03.011
Citation: YANG Liling, HUANG Mianjia, ZHANG Xiyan, GAO Zhufeng. Optimization of Fermentation Conditions for Streptomyces Strain DJ15 with Biological Control of Banana Fusarium Wilt[J]. Journal of Tropical Biology, 2016, 7(3): 343-347. doi: 10.15886/j.cnki.rdswxb.2016.03.011
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