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Volume 7 Issue 3
Sep.  2016
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Article Navigation >  Journal of Tropical Biology  > 2016  >  7(3): 285-289

GAO Pin, WEI Jingguang, XU Meng, CHEN Xiuli, QIN Qiwei, ZHOU Yongcan. Molecular Cloning,Expression and Subcellular Localization of PKR from Epinephelus coioides[J]. Journal of Tropical Biology, 2016, 7(3): 285-289. doi: 10.15886/j.cnki.rdswxb.2016.03.001
Citation: GAO Pin, WEI Jingguang, XU Meng, CHEN Xiuli, QIN Qiwei, ZHOU Yongcan. Molecular Cloning,Expression and Subcellular Localization of PKR from Epinephelus coioides[J]. Journal of Tropical Biology, 2016, 7(3): 285-289. doi: 10.15886/j.cnki.rdswxb.2016.03.001
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Molecular Cloning,Expression and Subcellular Localization of PKR from Epinephelus coioides

doi: 10.15886/j.cnki.rdswxb.2016.03.001

  • 1. College of Marine Sciences, Hainan University/Hainan Key Laboratory of Tropical Aquatic Biotechnology, Haikou, Hainan 570228, China;

  • 2. Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301;

  • 3. Guangdong Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong 510301, China

  • Received Date: 2015-07-26
  • The full length c DNA of PKR gene was cloned from the liver of grouper,Epinephelus coioides,which is 2 151 bp,including 1 863 bp ORF area,encoding 621 amino acids. Its protein molecular weight is 70 157.15 Daltons and PI is 5. 72. BLAST comparison on the NCBI web site found that the PKR of the grouper was highly homologous to that of other species. By constructing prokaryotic expression vector PKR-p ET-32 a,the fusion protein of PKR was obtained and purified. The mouse antiserum of PKR was also prepared. Real-time PCR analysis indicates that the PKR gene is expressed in all tissues of the grouper,most highly in the intestine and then in the head kidney. Subcellular localization of PKR showed that PKR was distributed in both nucleus and cytoplasm,but mainly in the cytoplasm.
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Molecular Cloning,Expression and Subcellular Localization of PKR from Epinephelus coioides

doi: 10.15886/j.cnki.rdswxb.2016.03.001
  • 1. College of Marine Sciences, Hainan University/Hainan Key Laboratory of Tropical Aquatic Biotechnology, Haikou, Hainan 570228, China;
  • 2. Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301;
  • 3. Guangdong Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong 510301, China
  • Received Date: 2015-07-26

Abstract: The full length c DNA of PKR gene was cloned from the liver of grouper,Epinephelus coioides,which is 2 151 bp,including 1 863 bp ORF area,encoding 621 amino acids. Its protein molecular weight is 70 157.15 Daltons and PI is 5. 72. BLAST comparison on the NCBI web site found that the PKR of the grouper was highly homologous to that of other species. By constructing prokaryotic expression vector PKR-p ET-32 a,the fusion protein of PKR was obtained and purified. The mouse antiserum of PKR was also prepared. Real-time PCR analysis indicates that the PKR gene is expressed in all tissues of the grouper,most highly in the intestine and then in the head kidney. Subcellular localization of PKR showed that PKR was distributed in both nucleus and cytoplasm,but mainly in the cytoplasm.

GAO Pin, WEI Jingguang, XU Meng, CHEN Xiuli, QIN Qiwei, ZHOU Yongcan. Molecular Cloning,Expression and Subcellular Localization of PKR from Epinephelus coioides[J]. Journal of Tropical Biology, 2016, 7(3): 285-289. doi: 10.15886/j.cnki.rdswxb.2016.03.001
Citation: GAO Pin, WEI Jingguang, XU Meng, CHEN Xiuli, QIN Qiwei, ZHOU Yongcan. Molecular Cloning,Expression and Subcellular Localization of PKR from Epinephelus coioides[J]. Journal of Tropical Biology, 2016, 7(3): 285-289. doi: 10.15886/j.cnki.rdswxb.2016.03.001
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