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Volume 7 Issue 3
Sep.  2016
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Article Navigation >  Journal of Tropical Biology  > 2016  >  7(3): 376-380

HOU Xiaowan, ZHANG Peiwei, SUN Peiwen, MENG Hui, ZHANG Zheng. Improved Method for Extracting High Quality Total RNA from Different Stem Parts of Aquilaria sinensis[J]. Journal of Tropical Biology, 2016, 7(3): 376-380. doi: 10.15886/j.cnki.rdswxb.2016.03.018
Citation: HOU Xiaowan, ZHANG Peiwei, SUN Peiwen, MENG Hui, ZHANG Zheng. Improved Method for Extracting High Quality Total RNA from Different Stem Parts of Aquilaria sinensis[J]. Journal of Tropical Biology, 2016, 7(3): 376-380. doi: 10.15886/j.cnki.rdswxb.2016.03.018
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Improved Method for Extracting High Quality Total RNA from Different Stem Parts of Aquilaria sinensis

doi: 10.15886/j.cnki.rdswxb.2016.03.018

  • 1. South subtropical crops research institute, Chinese academy of tropical agricultural sciences, Zhanjiang, Guangdong 524091, China;

  • 2. Hainan Branch, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou, Hainan 570311;

  • 3. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China

  • Received Date: 2016-01-16
  • A ten-year-old plant of agarwood（ Aquilaria sinensis） was induced by liquid transfusion technology,using healthy layer,transition and agarwood layers of the stems transfusion treated for 15 days as materials. Aidlab EASY-spin plus RN38 RNA extraction kits was modified in many key steps to improve the total RNA product qualities which were analyzed and then compared with those of the unmodified RNA extraction method. The results showed that the total RNA extracted by the modified RNA extraction method had better quality,purity and integrity than that of the unmodified method,in which the 28 S and 18 S bands were more legible with no impurity nor degradation,A260/ A280 was approximated to 2. 0,and the RIN greater than 6. This suggests that the modified method for extracting total RNA in different A. sinensis tissues transfusion treated produces high quality total RNA which can be used for transcriptome data analysis.
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Improved Method for Extracting High Quality Total RNA from Different Stem Parts of Aquilaria sinensis

doi: 10.15886/j.cnki.rdswxb.2016.03.018
  • 1. South subtropical crops research institute, Chinese academy of tropical agricultural sciences, Zhanjiang, Guangdong 524091, China;
  • 2. Hainan Branch, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou, Hainan 570311;
  • 3. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
  • Received Date: 2016-01-16

Abstract: A ten-year-old plant of agarwood（ Aquilaria sinensis） was induced by liquid transfusion technology,using healthy layer,transition and agarwood layers of the stems transfusion treated for 15 days as materials. Aidlab EASY-spin plus RN38 RNA extraction kits was modified in many key steps to improve the total RNA product qualities which were analyzed and then compared with those of the unmodified RNA extraction method. The results showed that the total RNA extracted by the modified RNA extraction method had better quality,purity and integrity than that of the unmodified method,in which the 28 S and 18 S bands were more legible with no impurity nor degradation,A260/ A280 was approximated to 2. 0,and the RIN greater than 6. This suggests that the modified method for extracting total RNA in different A. sinensis tissues transfusion treated produces high quality total RNA which can be used for transcriptome data analysis.

HOU Xiaowan, ZHANG Peiwei, SUN Peiwen, MENG Hui, ZHANG Zheng. Improved Method for Extracting High Quality Total RNA from Different Stem Parts of Aquilaria sinensis[J]. Journal of Tropical Biology, 2016, 7(3): 376-380. doi: 10.15886/j.cnki.rdswxb.2016.03.018
Citation: HOU Xiaowan, ZHANG Peiwei, SUN Peiwen, MENG Hui, ZHANG Zheng. Improved Method for Extracting High Quality Total RNA from Different Stem Parts of Aquilaria sinensis[J]. Journal of Tropical Biology, 2016, 7(3): 376-380. doi: 10.15886/j.cnki.rdswxb.2016.03.018
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