2010 Vol. 1, No. 2
2010, 1(2): 99-104.
doi: 10.15886/j.cnki.rdswxb.2010.02.012
Abstract:
Single factor experiment was used to test the effect factors of ISSR-PCR reaction system.After the optimization,a suitable ISSR-PCR reaction system of Ligustrum lucidum Ait.was established as follows:10 × buffer 2.5 μL,2.0 mmol·L-1 MgCl2,200 μmol·L-1...
Single factor experiment was used to test the effect factors of ISSR-PCR reaction system.After the optimization,a suitable ISSR-PCR reaction system of Ligustrum lucidum Ait.was established as follows:10 × buffer 2.5 μL,2.0 mmol·L-1 MgCl2,200 μmol·L-1...
2010, 1(2): 105-109.
doi: 10.15886/j.cnki.rdswxb.2010.02.001
Abstract:
According to the homologous sequences from Atriplex gmelini,Populus euphratica,Tetragonia tetragonioides,Kalidium foliatum,Populus tomentosa and Salicornia europ,PCR primers were designed and RT-PCR and RACE were used to amplify NHX1 DNA fragment fro...
According to the homologous sequences from Atriplex gmelini,Populus euphratica,Tetragonia tetragonioides,Kalidium foliatum,Populus tomentosa and Salicornia europ,PCR primers were designed and RT-PCR and RACE were used to amplify NHX1 DNA fragment fro...
2010, 1(2): 110-113.
doi: 10.15886/j.cnki.rdswxb.2010.02.008
Abstract:
To elucidate the molecular mechanism of cold damage to banana,suppression subtractive hybridization(SSH) library was successfully constructed under low temperature stress and the natural growth conditions.The library was analyzed by the blue & white ...
To elucidate the molecular mechanism of cold damage to banana,suppression subtractive hybridization(SSH) library was successfully constructed under low temperature stress and the natural growth conditions.The library was analyzed by the blue & white ...
2010, 1(2): 114-120.
doi: 10.15886/j.cnki.rdswxb.2010.02.018
Abstract:
According to TMEM130 gene sequence(NM-152913) information,human brain cDNA library was used as the template and the specific primers were designed.The nPCR amplification product was separated by agarose gel and the band was cloned into PMD18T-vector ...
According to TMEM130 gene sequence(NM-152913) information,human brain cDNA library was used as the template and the specific primers were designed.The nPCR amplification product was separated by agarose gel and the band was cloned into PMD18T-vector ...
2010, 1(2): 121-124.
doi: 10.15886/j.cnki.rdswxb.2010.02.017
Abstract:
To analysis the damages of the cooking oil fumes on the lung tissue and liver tissue,the activities of superoxide dismutase(SOD) and the concentration of malondialdehyde(MDA) in serum,liver tissue and lung tissue were determined.The results indicated...
To analysis the damages of the cooking oil fumes on the lung tissue and liver tissue,the activities of superoxide dismutase(SOD) and the concentration of malondialdehyde(MDA) in serum,liver tissue and lung tissue were determined.The results indicated...
2010, 1(2): 125-129.
doi: 10.15886/j.cnki.rdswxb.2010.02.005
Abstract:
The effects of different concentrations of chitosan on the antifungal activity against six different plant Fusarium oxysporum were investigated.The results indicated that the chitosan have antifungal activities within certain concentration range,and ...
The effects of different concentrations of chitosan on the antifungal activity against six different plant Fusarium oxysporum were investigated.The results indicated that the chitosan have antifungal activities within certain concentration range,and ...
2010, 1(2): 130-133.
doi: 10.15886/j.cnki.rdswxb.2010.02.006
Abstract:
The species and hazards of the sweet corn fruit borers in main planting areas in Hainan province,such as Qionghai,Ledong,Sanya and so on,were investigated.The results indicated that there are 4 specie insect on corn ear,Ostrinia furnacalis(Guenée),My...
The species and hazards of the sweet corn fruit borers in main planting areas in Hainan province,such as Qionghai,Ledong,Sanya and so on,were investigated.The results indicated that there are 4 specie insect on corn ear,Ostrinia furnacalis(Guenée),My...
2010, 1(2): 134-137.
doi: 10.15886/j.cnki.rdswxb.2010.02.009
Abstract:
A new recorded disease of banana in China was found when the species and damages of banana leaf spot disease in Hainan were investigated.Based on the results of tissue isolating,Koch’s postulates,morphology identification,rDNA-ITS sequence cloning an...
A new recorded disease of banana in China was found when the species and damages of banana leaf spot disease in Hainan were investigated.Based on the results of tissue isolating,Koch’s postulates,morphology identification,rDNA-ITS sequence cloning an...
2010, 1(2): 138-143.
doi: 10.15886/j.cnki.rdswxb.2010.02.007
Abstract:
Biomass,chlorophyll content,soluble sugars e.g.,9 indexes were used as the standard to evaluate the adaptation of the 10 Stylosanthes guianensis cv.Reyan species to the undernourished sand environment.The comprehensive evaluation results indicated th...
Biomass,chlorophyll content,soluble sugars e.g.,9 indexes were used as the standard to evaluate the adaptation of the 10 Stylosanthes guianensis cv.Reyan species to the undernourished sand environment.The comprehensive evaluation results indicated th...
2010, 1(2): 144-149.
doi: 10.15886/j.cnki.rdswxb.2010.02.010
Abstract:
Young leaves of mango potted young trees were used as materials to analysis the damages and protection response under the enhanced UV-B radiation.The results showed that the content of MDA and the relative conductivity increase,while the content of c...
Young leaves of mango potted young trees were used as materials to analysis the damages and protection response under the enhanced UV-B radiation.The results showed that the content of MDA and the relative conductivity increase,while the content of c...
2010, 1(2): 150-157.
doi: 10.15886/j.cnki.rdswxb.2010.02.019
Abstract:
The objective of our study was to forecast the banana production in the world and some countries and regions and provide basal data for the decision-making of banana production.The forecasting during 2011 to 2035 was given in detail.The banana produc...
The objective of our study was to forecast the banana production in the world and some countries and regions and provide basal data for the decision-making of banana production.The forecasting during 2011 to 2035 was given in detail.The banana produc...
2010, 1(2): 158-164.
doi: 10.15886/j.cnki.rdswxb.2010.02.011
Abstract:
The immature seeds of tree orchid(Epidendrum schbomburgkii) were used as materials,and the media formulas which were suitable for the induction,proliferation,germination of the protocorn and root growth were screened.The results revealed that after t...
The immature seeds of tree orchid(Epidendrum schbomburgkii) were used as materials,and the media formulas which were suitable for the induction,proliferation,germination of the protocorn and root growth were screened.The results revealed that after t...
2010, 1(2): 165-169.
doi: 10.15886/j.cnki.rdswxb.2010.02.014
Abstract:
The pathogenic Vibrio harveyi was intraperitoneal injected to Epinephelus bleekeri,and after 18h,the crude extract was prepared from the head-kidney of E.bleekeri,and the analysis results of the antibacterial activities of the crude head-kidney extra...
The pathogenic Vibrio harveyi was intraperitoneal injected to Epinephelus bleekeri,and after 18h,the crude extract was prepared from the head-kidney of E.bleekeri,and the analysis results of the antibacterial activities of the crude head-kidney extra...
2010, 1(2): 170-174.
doi: 10.15886/j.cnki.rdswxb.2010.02.015
Abstract:
The general practice on division of the postembryonic development period of osteichthyes was used as reference,based on the morphology characteristic,the postembryonic development period of Plectropomus leopardus was divided into larval stage(includi...
The general practice on division of the postembryonic development period of osteichthyes was used as reference,based on the morphology characteristic,the postembryonic development period of Plectropomus leopardus was divided into larval stage(includi...
2010, 1(2): 175-182.
doi: 10.15886/j.cnki.rdswxb.2010.02.016
Abstract:
From 2000 to 2009,the large-scale bottom perch seaweed resource in Hainan Island was investigated,and 51 kinds common red alga,17 kinds brown alga and 15 kinds green alga were discovered.
From 2000 to 2009,the large-scale bottom perch seaweed resource in Hainan Island was investigated,and 51 kinds common red alga,17 kinds brown alga and 15 kinds green alga were discovered.
2010, 1(2): 183-186,192.
doi: 10.15886/j.cnki.rdswxb.2010.02.013
Abstract:
50 ISSR primers were used in the ISSR-PCR amplification for 12 germplasm materials of Ligustrum lucidum Ait.from different areas,and 12 effective ISSR primers which were suitable for all gerplasm materials of Ligustrum lucidum Ait.were selected out,w...
50 ISSR primers were used in the ISSR-PCR amplification for 12 germplasm materials of Ligustrum lucidum Ait.from different areas,and 12 effective ISSR primers which were suitable for all gerplasm materials of Ligustrum lucidum Ait.were selected out,w...
2010, 1(2): 187-192.
doi: 10.15886/j.cnki.rdswxb.2010.02.003
Abstract:
As biomass and reproduction resources for sustainable development,cellulose has been paid more and more attentions.Because of its high polymerization and crystallites degree,cellulose is difficult to dissolve in common solvents.The development of new...
As biomass and reproduction resources for sustainable development,cellulose has been paid more and more attentions.Because of its high polymerization and crystallites degree,cellulose is difficult to dissolve in common solvents.The development of new...
2010, 1(2): 193-196.
doi: 10.15886/j.cnki.rdswxb.2010.02.002
Abstract:
As a kind heat shock protein with about 15 KD-30KD molecular weight,Small Heat Shock Proteins(sHSPs) in plant plays an important role in heat-resistance.In the paper,the sequence phylogenetic relationships of various sHSPs in the four common nightsha...
As a kind heat shock protein with about 15 KD-30KD molecular weight,Small Heat Shock Proteins(sHSPs) in plant plays an important role in heat-resistance.In the paper,the sequence phylogenetic relationships of various sHSPs in the four common nightsha...