Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code

2010 Vol. 1, No. 2

Establishment and Optimization of ISSR-PCR Reaction System of Ligustrum lucidum Ait.
LI Chuan-dai, CHEN Fei, WANG Tian-di, LIU Guo-min
2010, 1(2): 99-104. doi: 10.15886/j.cnki.rdswxb.2010.02.012
Abstract:
Single factor experiment was used to test the effect factors of ISSR-PCR reaction system.After the optimization,a suitable ISSR-PCR reaction system of Ligustrum lucidum Ait.was established as follows:10 × buffer 2.5 μL,2.0 mmol·L-1 MgCl2,200 μmol·L-1...
Cloning of Na+/H+ Antiporter Gene from Bruguiera gymnorrhiza(L.) LAM
GUO Qing-shui, XU Li-xin, YUAN Qian-hua, YU Wei
2010, 1(2): 105-109. doi: 10.15886/j.cnki.rdswxb.2010.02.001
Abstract:
According to the homologous sequences from Atriplex gmelini,Populus euphratica,Tetragonia tetragonioides,Kalidium foliatum,Populus tomentosa and Salicornia europ,PCR primers were designed and RT-PCR and RACE were used to amplify NHX1 DNA fragment fro...
Construction of cDNA Subtractive Library of Banana Leaf under Low Temperature Stress
QI Jun-feng, FENG Ren-jun, CHENG Ping, YUAN Ke-hua, ZHANG Li-li, ZHANG Yin-dong
2010, 1(2): 110-113. doi: 10.15886/j.cnki.rdswxb.2010.02.008
Abstract:
To elucidate the molecular mechanism of cold damage to banana,suppression subtractive hybridization(SSH) library was successfully constructed under low temperature stress and the natural growth conditions.The library was analyzed by the blue & white ...
Cloning and Bioinformatics Analysis of Human Polycystic Kidney Disease-related Protein TMEM130
QI Xing-zhu, HU Wen-ting, ZHANG Jun-fang, HUANG Jun-sheng, LI Jun
2010, 1(2): 114-120. doi: 10.15886/j.cnki.rdswxb.2010.02.018
Abstract:
According to TMEM130 gene sequence(NM-152913) information,human brain cDNA library was used as the template and the specific primers were designed.The nPCR amplification product was separated by agarose gel and the band was cloned into PMD18T-vector ...
Damages of the Liver and Lung Tissue of Mice Caused by Cooking Flaxseed Oil Fumes
WANG Xiao-bo, GAO Jian-pei, WANG Bin, HU Yan-qin, LUO Ling-ling
2010, 1(2): 121-124. doi: 10.15886/j.cnki.rdswxb.2010.02.017
Abstract:
To analysis the damages of the cooking oil fumes on the lung tissue and liver tissue,the activities of superoxide dismutase(SOD) and the concentration of malondialdehyde(MDA) in serum,liver tissue and lung tissue were determined.The results indicated...
Study on Antifungal Activity of Chitosan against Fusarium oxysporum
LIU Chang-yan, JIN Ling, LIU Ying-ying, WANG Jun, HUANG Jun-sheng
2010, 1(2): 125-129. doi: 10.15886/j.cnki.rdswxb.2010.02.005
Abstract:
The effects of different concentrations of chitosan on the antifungal activity against six different plant Fusarium oxysporum were investigated.The results indicated that the chitosan have antifungal activities within certain concentration range,and ...
Investigation on the Species and Hazards of Sweet Corn Fruit Borers
LIN Zhu-feng, WANG Yun-qin, XIE Sheng-hua, CHEN Hai-yan, JI Xun-cong
2010, 1(2): 130-133. doi: 10.15886/j.cnki.rdswxb.2010.02.006
Abstract:
The species and hazards of the sweet corn fruit borers in main planting areas in Hainan province,such as Qionghai,Ledong,Sanya and so on,were investigated.The results indicated that there are 4 specie insect on corn ear,Ostrinia furnacalis(Guenée),My...
A New Leaf Spot Disease of Banana Caused by Pyricularia angulata
ZHANG He, ZHANG Xin, QI Yan-xiang, PU Jinji, LV Yan-chao, XIE Yi-xian
2010, 1(2): 134-137. doi: 10.15886/j.cnki.rdswxb.2010.02.009
Abstract:
A new recorded disease of banana in China was found when the species and damages of banana leaf spot disease in Hainan were investigated.Based on the results of tissue isolating,Koch’s postulates,morphology identification,rDNA-ITS sequence cloning an...
Adaptation of the Stylosanthes guianensis cv. Reyan Species to Sand Environment
LIU Zhan-lin, DUAN Rui-jun, LIU Guo-dao, FU Shao-ping, GUO Jian-chun
2010, 1(2): 138-143. doi: 10.15886/j.cnki.rdswxb.2010.02.007
Abstract:
Biomass,chlorophyll content,soluble sugars e.g.,9 indexes were used as the standard to evaluate the adaptation of the 10 Stylosanthes guianensis cv.Reyan species to the undernourished sand environment.The comprehensive evaluation results indicated th...
Study on the Damages and Protection Response of Young Leaves of Mango under Enhanced UV-B Radiation
LIU Peng, PAN Xue-feng, ZHOU Kai-bing
2010, 1(2): 144-149. doi: 10.15886/j.cnki.rdswxb.2010.02.010
Abstract:
Young leaves of mango potted young trees were used as materials to analysis the damages and protection response under the enhanced UV-B radiation.The results showed that the content of MDA and the relative conductivity increase,while the content of c...
Forecasting on Banana Production Worldwide
LIU Guang-hua, ZHANG Xi-yan, ZHANG Wen-jun
2010, 1(2): 150-157. doi: 10.15886/j.cnki.rdswxb.2010.02.019
Abstract:
The objective of our study was to forecast the banana production in the world and some countries and regions and provide basal data for the decision-making of banana production.The forecasting during 2011 to 2035 was given in detail.The banana produc...
Rapid Propagation of Immature Seeds of Tree Orchid(Epidendrum schbomburgkiiin vitro
PAN Xue-feng, LING Mei, WANG An-shi
2010, 1(2): 158-164. doi: 10.15886/j.cnki.rdswxb.2010.02.011
Abstract:
The immature seeds of tree orchid(Epidendrum schbomburgkii) were used as materials,and the media formulas which were suitable for the induction,proliferation,germination of the protocorn and root growth were screened.The results revealed that after t...
Study on the Antibacterial Head & Kidney Extract of Epinephelus bleekeri Induced by Vibrio harveyi
CHEN Zhao, WANG Shi-feng, XIE Zhen-yu, YU Wen-yuan, ZHOU Yong-can
2010, 1(2): 165-169. doi: 10.15886/j.cnki.rdswxb.2010.02.014
Abstract:
The pathogenic Vibrio harveyi was intraperitoneal injected to Epinephelus bleekeri,and after 18h,the crude extract was prepared from the head-kidney of E.bleekeri,and the analysis results of the antibacterial activities of the crude head-kidney extra...
Observation on the Morphology of the Larva,Juvenile and Young Fish of Plectropomus leopardus Lacépède
FU Shu-yuan, WANG Yong-bo, ZHENG Fei, WANG Guo-fu, LI Xiang-min
2010, 1(2): 170-174. doi: 10.15886/j.cnki.rdswxb.2010.02.015
Abstract:
The general practice on division of the postembryonic development period of osteichthyes was used as reference,based on the morphology characteristic,the postembryonic development period of Plectropomus leopardus was divided into larval stage(includi...
Common Bottom Perch Seaweed in Hainan Island
WANG Hong-yong, WU Hong-liu, YAO Xue-mei, FENG Yong-qin, WEN Zhen, XIE Gui-lian, PENG Hui
2010, 1(2): 175-182. doi: 10.15886/j.cnki.rdswxb.2010.02.016
Abstract:
From 2000 to 2009,the large-scale bottom perch seaweed resource in Hainan Island was investigated,and 51 kinds common red alga,17 kinds brown alga and 15 kinds green alga were discovered.
Primer Screening in the Study on Ligustrum lucidum Ait. with ISSR Markers
LI Chuan-dai
2010, 1(2): 183-186,192. doi: 10.15886/j.cnki.rdswxb.2010.02.013
Abstract:
50 ISSR primers were used in the ISSR-PCR amplification for 12 germplasm materials of Ligustrum lucidum Ait.from different areas,and 12 effective ISSR primers which were suitable for all gerplasm materials of Ligustrum lucidum Ait.were selected out,w...
Progresses of Cellulose Solvent
XU Tian-jun, FENG Yu-hong, PANG Su-juan
2010, 1(2): 187-192. doi: 10.15886/j.cnki.rdswxb.2010.02.003
Abstract:
As biomass and reproduction resources for sustainable development,cellulose has been paid more and more attentions.Because of its high polymerization and crystallites degree,cellulose is difficult to dissolve in common solvents.The development of new...
Progresses on The Evolution and Expression Regulation of Small Heat Shock Proteins in Plant
LI Chun-zi, CHENG Shan-han
2010, 1(2): 193-196. doi: 10.15886/j.cnki.rdswxb.2010.02.002
Abstract:
As a kind heat shock protein with about 15 KD-30KD molecular weight,Small Heat Shock Proteins(sHSPs) in plant plays an important role in heat-resistance.In the paper,the sequence phylogenetic relationships of various sHSPs in the four common nightsha...