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Volume 1 Issue 2
Jun.  2010
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Article Navigation >  Journal of Tropical Biology  > 2010  >  1(2): 105-109

GUO Qing-shui, XU Li-xin, YUAN Qian-hua, YU Wei. Cloning of Na+/H+ Antiporter Gene from Bruguiera gymnorrhiza（L.） LAM[J]. Journal of Tropical Biology, 2010, 1(2): 105-109. doi: 10.15886/j.cnki.rdswxb.2010.02.001
Citation: GUO Qing-shui, XU Li-xin, YUAN Qian-hua, YU Wei. Cloning of Na+/H+ Antiporter Gene from Bruguiera gymnorrhiza（L.） LAM[J]. Journal of Tropical Biology, 2010, 1(2): 105-109. doi: 10.15886/j.cnki.rdswxb.2010.02.001
shu

Cloning of Na+/H+ Antiporter Gene from Bruguiera gymnorrhiza（L.） LAM

doi: 10.15886/j.cnki.rdswxb.2010.02.001

  • Agricultural College, Hainan University, Haikou 570228, China

  • Received Date: 2009-12-14
  • According to the homologous sequences from Atriplex gmelini,Populus euphratica,Tetragonia tetragonioides,Kalidium foliatum,Populus tomentosa and Salicornia europ,PCR primers were designed and RT-PCR and RACE were used to amplify NHX1 DNA fragment from the leaves of Bruguiera gymnorrhiza.The results indicated that the BgNHX1 contains 2 130 bp,including an open reading frame of 1 626 bp,which encodes a predicted polypeptide of 541 amino acids,and there are 12 predicted transmembrane domains.Base on the data that the sequence showed high homologies（＞ 80%） with Na + /H + antiporter genes from Ricinus communis Populus euphratica,Citrus,paradisi,vitis vinifera,it was very possible that BgNHX1 was competent for Na + compartmentation in cells.
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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Cloning of Na+/H+ Antiporter Gene from Bruguiera gymnorrhiza（L.） LAM

doi: 10.15886/j.cnki.rdswxb.2010.02.001
  • Agricultural College, Hainan University, Haikou 570228, China
  • Received Date: 2009-12-14

Abstract: According to the homologous sequences from Atriplex gmelini,Populus euphratica,Tetragonia tetragonioides,Kalidium foliatum,Populus tomentosa and Salicornia europ,PCR primers were designed and RT-PCR and RACE were used to amplify NHX1 DNA fragment from the leaves of Bruguiera gymnorrhiza.The results indicated that the BgNHX1 contains 2 130 bp,including an open reading frame of 1 626 bp,which encodes a predicted polypeptide of 541 amino acids,and there are 12 predicted transmembrane domains.Base on the data that the sequence showed high homologies（＞ 80%） with Na + /H + antiporter genes from Ricinus communis Populus euphratica,Citrus,paradisi,vitis vinifera,it was very possible that BgNHX1 was competent for Na + compartmentation in cells.

GUO Qing-shui, XU Li-xin, YUAN Qian-hua, YU Wei. Cloning of Na+/H+ Antiporter Gene from Bruguiera gymnorrhiza（L.） LAM[J]. Journal of Tropical Biology, 2010, 1(2): 105-109. doi: 10.15886/j.cnki.rdswxb.2010.02.001
Citation: GUO Qing-shui, XU Li-xin, YUAN Qian-hua, YU Wei. Cloning of Na+/H+ Antiporter Gene from Bruguiera gymnorrhiza（L.） LAM[J]. Journal of Tropical Biology, 2010, 1(2): 105-109. doi: 10.15886/j.cnki.rdswxb.2010.02.001
shu

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