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Volume 1 Issue 2
Jun.  2010
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Article Navigation >  Journal of Tropical Biology  > 2010  >  1(2): 110-113

QI Jun-feng, FENG Ren-jun, CHENG Ping, YUAN Ke-hua, ZHANG Li-li, ZHANG Yin-dong. Construction of cDNA Subtractive Library of Banana Leaf under Low Temperature Stress[J]. Journal of Tropical Biology, 2010, 1(2): 110-113. doi: 10.15886/j.cnki.rdswxb.2010.02.008
Citation: QI Jun-feng, FENG Ren-jun, CHENG Ping, YUAN Ke-hua, ZHANG Li-li, ZHANG Yin-dong. Construction of cDNA Subtractive Library of Banana Leaf under Low Temperature Stress[J]. Journal of Tropical Biology, 2010, 1(2): 110-113. doi: 10.15886/j.cnki.rdswxb.2010.02.008
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Construction of cDNA Subtractive Library of Banana Leaf under Low Temperature Stress

doi: 10.15886/j.cnki.rdswxb.2010.02.008

  • 1. Agricultural College, Hainan University, Haikou 570228, China;

  • 2. Institute of Tropical Bioscience and Biotechnology, CATAS, Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Haikou 571101, China

  • Received Date: 2010-02-27
  • To elucidate the molecular mechanism of cold damage to banana,suppression subtractive hybridization（SSH） library was successfully constructed under low temperature stress and the natural growth conditions.The library was analyzed by the blue ＆ white spot screening,cloning,sequencing and semi-qPCR for No.28,and the results indicated that there may be some unknown gene involving the anti-cold of banana except No.28,and it is very possible to obtain more specific express gene under low temperature stress.
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Construction of cDNA Subtractive Library of Banana Leaf under Low Temperature Stress

doi: 10.15886/j.cnki.rdswxb.2010.02.008
  • 1. Agricultural College, Hainan University, Haikou 570228, China;
  • 2. Institute of Tropical Bioscience and Biotechnology, CATAS, Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Haikou 571101, China
  • Received Date: 2010-02-27

Abstract: To elucidate the molecular mechanism of cold damage to banana,suppression subtractive hybridization（SSH） library was successfully constructed under low temperature stress and the natural growth conditions.The library was analyzed by the blue ＆ white spot screening,cloning,sequencing and semi-qPCR for No.28,and the results indicated that there may be some unknown gene involving the anti-cold of banana except No.28,and it is very possible to obtain more specific express gene under low temperature stress.

QI Jun-feng, FENG Ren-jun, CHENG Ping, YUAN Ke-hua, ZHANG Li-li, ZHANG Yin-dong. Construction of cDNA Subtractive Library of Banana Leaf under Low Temperature Stress[J]. Journal of Tropical Biology, 2010, 1(2): 110-113. doi: 10.15886/j.cnki.rdswxb.2010.02.008
Citation: QI Jun-feng, FENG Ren-jun, CHENG Ping, YUAN Ke-hua, ZHANG Li-li, ZHANG Yin-dong. Construction of cDNA Subtractive Library of Banana Leaf under Low Temperature Stress[J]. Journal of Tropical Biology, 2010, 1(2): 110-113. doi: 10.15886/j.cnki.rdswxb.2010.02.008
shu

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