Rapid Propagation of Immature Seeds of Tree Orchid（Epidendrum schbomburgkii） in vitro

Key words: 
• tree orchid（Epidendrum schbomburgkii） /
• seed /
• protocorn /
• tissue culture

Abstract: The immature seeds of tree orchid（Epidendrum schbomburgkii） were used as materials,and the media formulas which were suitable for the induction,proliferation,germination of the protocorn and root growth were screened.The results revealed that after the seeds of tree orchid was sown on the media MS + BA0.5 mg· L^-1 + NAA0.25 mg·L^-1 + sugar 25 g·L^-1,after 7—10 days,there are some green spots,after 25 days,the protocorn could been seen growing on it;the media 1 /2MS + 6-BA1.5 mg·L^-1 + NAA0.25 mg·L^-1 + sugar20 g·L^-1 + CH2.0 g·L^-1 is most suitable for the proliferation of protocorn,with the best effectiveness and the proliferating coefficient over 9 times;that the germination rate attain over 90% by implanting the protocorn in the media 1 /2MS + NAA0.1 mg·L^-1 + 6-BA0.2 mg·L^-1 + sugar 25 g·L^-1;the rooting percentage of 94% could also be obtained by implanting the germinated buds in the media 1 /2MS + NAA 0.5 mg·L^-1 + BA 0.1 mg·L^-1 + banana 70 g·L^-1 + sugar 25 g·L^-1 for rooting;during the period of transplantation,the moss is a satisfied medium for transplanting by which the survival rate can reach as high as 92.5%,and the plantlets vigorously grow.