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Volume 1 Issue 2
Jun.  2010
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Article Navigation >  Journal of Tropical Biology  > 2010  >  1(2): 183-186,192

LI Chuan-dai. Primer Screening in the Study on Ligustrum lucidum Ait. with ISSR Markers[J]. Journal of Tropical Biology, 2010, 1(2): 183-186,192. doi: 10.15886/j.cnki.rdswxb.2010.02.013
Citation: LI Chuan-dai. Primer Screening in the Study on Ligustrum lucidum Ait. with ISSR Markers[J]. Journal of Tropical Biology, 2010, 1(2): 183-186,192. doi: 10.15886/j.cnki.rdswxb.2010.02.013
shu

Primer Screening in the Study on Ligustrum lucidum Ait. with ISSR Markers

doi: 10.15886/j.cnki.rdswxb.2010.02.013

  • Agricultural College, Hainan University, Haikou 570228, China

  • Received Date: 2010-04-21
  • 50 ISSR primers were used in the ISSR-PCR amplification for 12 germplasm materials of Ligustrum lucidum Ait.from different areas,and 12 effective ISSR primers which were suitable for all gerplasm materials of Ligustrum lucidum Ait.were selected out,which were used in the ISSR-PCR amplification for 12 germplasm materials of Ligustrum lucidum Ait.The results indicated that 228 distinct DNA bands are obtained,and 210 bands are polymorphic,which amount to 92.1% of the total bands amplified.Averagely,19 bands could be obtained with each primer.The 12 primers selected could be effectively applied to ISSR analysis of the germplasm materials of Ligustrum lucidum Ait.
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Primer Screening in the Study on Ligustrum lucidum Ait. with ISSR Markers

doi: 10.15886/j.cnki.rdswxb.2010.02.013
  • Agricultural College, Hainan University, Haikou 570228, China
  • Received Date: 2010-04-21

Abstract: 50 ISSR primers were used in the ISSR-PCR amplification for 12 germplasm materials of Ligustrum lucidum Ait.from different areas,and 12 effective ISSR primers which were suitable for all gerplasm materials of Ligustrum lucidum Ait.were selected out,which were used in the ISSR-PCR amplification for 12 germplasm materials of Ligustrum lucidum Ait.The results indicated that 228 distinct DNA bands are obtained,and 210 bands are polymorphic,which amount to 92.1% of the total bands amplified.Averagely,19 bands could be obtained with each primer.The 12 primers selected could be effectively applied to ISSR analysis of the germplasm materials of Ligustrum lucidum Ait.

LI Chuan-dai. Primer Screening in the Study on Ligustrum lucidum Ait. with ISSR Markers[J]. Journal of Tropical Biology, 2010, 1(2): 183-186,192. doi: 10.15886/j.cnki.rdswxb.2010.02.013
Citation: LI Chuan-dai. Primer Screening in the Study on Ligustrum lucidum Ait. with ISSR Markers[J]. Journal of Tropical Biology, 2010, 1(2): 183-186,192. doi: 10.15886/j.cnki.rdswxb.2010.02.013
shu

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