东寨港红树林典型区域氯霉素抗性基因的分布

阮孙兰; 赵洪伟; 张禹; 张腾云; 张鸣珊; 靳晓拓; 黎平; 刁晓平

doi:10.15886/j.cnki.rdswxb.2018.02.004

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东寨港红树林典型区域氯霉素抗性基因的分布

阮孙兰, 赵洪伟, 张禹, 张腾云, 张鸣珊, 靳晓拓, 黎平, 刁晓平

文章导航 > 热带生物学报 > 2018 > 9(2): 147-155

阮孙兰, 赵洪伟, 张禹, 张腾云, 张鸣珊, 靳晓拓, 黎平, 刁晓平. 东寨港红树林典型区域氯霉素抗性基因的分布[J]. 热带生物学报, 2018, 9(2): 147-155. doi: 10.15886/j.cnki.rdswxb.2018.02.004

引用本文:

RUAN Sunlan, ZHAO Hongwei, ZHANG Yu, ZHANG Tengyun, ZHANG Mingshan, JIN Xiaotuo, LI Ping, DIAO Xiaoping. Distribution of Chloramphenicol Resistance Genes in the Typical Area of Mangroves in Dongzhai Harbor,Hainan,China[J]. Journal of Tropical Biology, 2018, 9(2): 147-155. doi: 10.15886/j.cnki.rdswxb.2018.02.004

Citation:

RUAN Sunlan, ZHAO Hongwei, ZHANG Yu, ZHANG Tengyun, ZHANG Mingshan, JIN Xiaotuo, LI Ping, DIAO Xiaoping. Distribution of Chloramphenicol Resistance Genes in the Typical Area of Mangroves in Dongzhai Harbor,Hainan,China[J]. Journal of Tropical Biology, 2018, 9(2): 147-155. doi: 10.15886/j.cnki.rdswxb.2018.02.004

东寨港红树林典型区域氯霉素抗性基因的分布

doi: 10.15886/j.cnki.rdswxb.2018.02.004

阮孙兰^1,2,3,
赵洪伟^1,2,3, ,,
张禹^1,2,3,
张腾云^1,2,3,
张鸣珊⁴,
靳晓拓^1,2,3,
黎平^1,2,3,
刁晓平^1,3

1. 海南大学南海海洋资源利用国家重点实验室, 海口 570228;
2. 海南大学海口市环境毒理学重点实验室, 海口 570228;
3. 海南大学热带农林学院, 海口 570228;
4. 海南省环境科学研究院/海南省环境监测中心, 海口 570228

基金项目:

海南省重点研发项目(ZDYF2017050)

海南省自然科学基金项目(417053,317302)

海南省重大科技计划项目(ZDKJ2017002)

详细信息

第一作者:
阮孙兰(1991-),女,海南大学热带农林学院2015级硕士研究生.E-mail:ruansunlan1991@163.com

通信作者:
赵洪伟(1983-),男,副教授,博士.研究方向:污染生态学.E-mail:hwzhao@hainu.edu.cn

中图分类号: X171

Distribution of Chloramphenicol Resistance Genes in the Typical Area of Mangroves in Dongzhai Harbor,Hainan,China

RUAN Sunlan^1,2,3,
ZHAO Hongwei^{1,2,3
, ,},
ZHANG Yu^1,2,3,
ZHANG Tengyun^1,2,3,
ZHANG Mingshan⁴,
JIN Xiaotuo^1,2,3,
LI Ping^1,2,3,
DIAO Xiaoping^1,3

1. State Key Laboratory of Marine Resource Utilization of South China Sea, Hainan University, Haikou, Hainan 570228, China;
2. Haikou Key Laboratory of Environmental Toxicology, Hainan University, Haikou, Hainan 570228, China;
3. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China;
4. Hainan Research Academy of Environmental Science & Hainan Environmental Monitoring Center, Haikou, Hainan 570228, China

摘要: 采用实时荧光定量PCR方法,于2017年8月采集东寨港红树林区域表层沉积物,探究氯霉素抗性基因在该区域的污染情况,同时比较红树区域密林内与密林外沉积物样品中氯霉素抗性基因的分布差异及其与环境因子之间的相关性。结果表明:该区域受氯霉素抗性基因污染严重,基因绝对丰度可高达108 copies·g^-1数量级;具有不同耐药机制的氯霉素抗性基因在环境样本中具有不同的组成比例,始终以cml基因居多,证明该地区的氯霉素抗性基因的耐药机制以抗生素主动外排为主;红树林密林内的沉积物样品中氯霉素抗性基因的绝对丰度比其他样点高;氯霉素抗性基因与环境因子的相关性分析显示亚硝酸盐(NO₂^--N)和氯霉素抗性基因具有极显著相关性。
- 氯霉素抗性基因 /
- 红树林 /
- 实时荧光定量PCR
Abstract: The long-term abuse of antibiotics has led to the rapid growth of drug-resistant bacteria in the environment. The distribution and mechanism of antibiotics resistance genes（ ARGs） have attracted public attention.ARGs,as an emerging contaminant,not only have a serious impact on the environment,but also pose a potential threat to the health of organisms and human beings. The surface sediment of the mangrove area in Dongzhai Harbor,Haikou,Hainan,China was collected in the mangroves,near the mangroves and the mud flats in August,2017 to determine the abundance of ARGs by using real-time quantitative PCR in a view to investigate the pollution of chloramphenicol resistance genes（ CRGs） in this area. The distribution of CRGs in the sediment samples in the mangroves,near the mangroves and in the mud flats was analyzed and compared,and the correlation between the CRGs and the environmental factors were analyzed. The results showed that this area was seriously polluted by CRGs,with an absolute abundance of CRGs being 108 copies · g^-1. CRGs with different resistance mechanisms had different compositional ratios in the environmental samples,of which the cml gene was always the highest in the compositional ratios of all the samples,indicating that the resistant mechanism of CRGs is based on active efflux of antibiotics. The sediment samples in the mangroves showed higher absolute abundance of CRGs than those near the mangroves or in the mud flats. Correlation analysis between the CRGs and the environmental factors showed that NO₂^--N was highly significantly correlated with the CRGs.
- chloramphenicol resistance genes /
- mangroves /
- real-time quantitative PCR

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东寨港红树林典型区域氯霉素抗性基因的分布

doi: 10.15886/j.cnki.rdswxb.2018.02.004

1. 海南大学南海海洋资源利用国家重点实验室, 海口 570228;

2. 海南大学海口市环境毒理学重点实验室, 海口 570228;

3. 海南大学热带农林学院, 海口 570228;

4. 海南省环境科学研究院/海南省环境监测中心, 海口 570228

基金项目:

海南省重点研发项目(ZDYF2017050)

海南省自然科学基金项目(417053,317302)

海南省重大科技计划项目(ZDKJ2017002)

作者简介:
阮孙兰(1991-),女,海南大学热带农林学院2015级硕士研究生.E-mail:ruansunlan1991@163.com

通讯作者: 赵洪伟(1983-),男,副教授,博士.研究方向:污染生态学.E-mail:hwzhao@hainu.edu.cn

收稿日期: 2018-01-04
修回日期: 2018-02-23

中图分类号: X171

关键词:

氯霉素抗性基因 /

红树林 /

实时荧光定量PCR

摘要: 采用实时荧光定量PCR方法,于2017年8月采集东寨港红树林区域表层沉积物,探究氯霉素抗性基因在该区域的污染情况,同时比较红树区域密林内与密林外沉积物样品中氯霉素抗性基因的分布差异及其与环境因子之间的相关性。结果表明:该区域受氯霉素抗性基因污染严重,基因绝对丰度可高达108 copies·g^-1数量级;具有不同耐药机制的氯霉素抗性基因在环境样本中具有不同的组成比例,始终以cml基因居多,证明该地区的氯霉素抗性基因的耐药机制以抗生素主动外排为主;红树林密林内的沉积物样品中氯霉素抗性基因的绝对丰度比其他样点高;氯霉素抗性基因与环境因子的相关性分析显示亚硝酸盐(NO₂^--N)和氯霉素抗性基因具有极显著相关性。

English Abstract

Distribution of Chloramphenicol Resistance Genes in the Typical Area of Mangroves in Dongzhai Harbor,Hainan,China

1. State Key Laboratory of Marine Resource Utilization of South China Sea, Hainan University, Haikou, Hainan 570228, China;

2. Haikou Key Laboratory of Environmental Toxicology, Hainan University, Haikou, Hainan 570228, China;

3. Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China;

4. Hainan Research Academy of Environmental Science & Hainan Environmental Monitoring Center, Haikou, Hainan 570228, China

Received Date: 2018-01-04
Rev Recd Date: 2018-02-23

Keywords:

Abstract: The long-term abuse of antibiotics has led to the rapid growth of drug-resistant bacteria in the environment. The distribution and mechanism of antibiotics resistance genes（ ARGs） have attracted public attention.ARGs,as an emerging contaminant,not only have a serious impact on the environment,but also pose a potential threat to the health of organisms and human beings. The surface sediment of the mangrove area in Dongzhai Harbor,Haikou,Hainan,China was collected in the mangroves,near the mangroves and the mud flats in August,2017 to determine the abundance of ARGs by using real-time quantitative PCR in a view to investigate the pollution of chloramphenicol resistance genes（ CRGs） in this area. The distribution of CRGs in the sediment samples in the mangroves,near the mangroves and in the mud flats was analyzed and compared,and the correlation between the CRGs and the environmental factors were analyzed. The results showed that this area was seriously polluted by CRGs,with an absolute abundance of CRGs being 108 copies · g^-1. CRGs with different resistance mechanisms had different compositional ratios in the environmental samples,of which the cml gene was always the highest in the compositional ratios of all the samples,indicating that the resistant mechanism of CRGs is based on active efflux of antibiotics. The sediment samples in the mangroves showed higher absolute abundance of CRGs than those near the mangroves or in the mud flats. Correlation analysis between the CRGs and the environmental factors showed that NO₂^--N was highly significantly correlated with the CRGs.

引用本文:

Citation:

RUAN Sunlan, ZHAO Hongwei, ZHANG Yu, ZHANG Tengyun, ZHANG Mingshan, JIN Xiaotuo, LI Ping, DIAO Xiaoping. Distribution of Chloramphenicol Resistance Genes in the Typical Area of Mangroves in Dongzhai Harbor,Hainan,China[J]. Journal of Tropical Biology, 2018, 9(2): 147-155. doi: 10.15886/j.cnki.rdswxb.2018.02.004