2017 Vol. 8, No. 2
2017, 8(2): 133-140.
doi: 10.15886/j.cnki.rdswxb.2017.02.001
Abstract
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Abstract:
ICE（inducer of CBF expression）-mediated ABA-independent signal transduction plays an important role in regulating the responses of plants to cold stress. A c DNA of HbICE2（ Gene Bank Accession No. :KY406163） was cloned from the leaf samples of rubber tree clone Reyan 8-79 by using RT-PCR. The HbICE2 was 1 677 base pairs（ bp） in length,containing a 1 593 bp open reading frame（ ORF）. The encoded protein,HbICE2,contained 530 amino acid residues with a predicted molecular mass of 58. 02 KDa and a pI of 5. 95.The HbICE2 had a b HLH domain with a specific highly conserved amino acid motif（ KMDRASILGDAI（ D/E）YLKELL）. Real-time quantitative PCR analysis showed that HbICE2 was mainly expressed in the leaf and bark among the samples tested. Its expression level was significantly higher in the original bark than in the regenerated bark,and significantly higher in the bark of the highly cold tolerant rubber clone PR107 that in that of the less cold tolerant clone Reyan 8-79. This suggests that HbICE2 may be crucial in regulating the cold tolerance of the bark of the rubber tree.
ICE（inducer of CBF expression）-mediated ABA-independent signal transduction plays an important role in regulating the responses of plants to cold stress. A c DNA of HbICE2（ Gene Bank Accession No. :KY406163） was cloned from the leaf samples of rubber tree clone Reyan 8-79 by using RT-PCR. The HbICE2 was 1 677 base pairs（ bp） in length,containing a 1 593 bp open reading frame（ ORF）. The encoded protein,HbICE2,contained 530 amino acid residues with a predicted molecular mass of 58. 02 KDa and a pI of 5. 95.The HbICE2 had a b HLH domain with a specific highly conserved amino acid motif（ KMDRASILGDAI（ D/E）YLKELL）. Real-time quantitative PCR analysis showed that HbICE2 was mainly expressed in the leaf and bark among the samples tested. Its expression level was significantly higher in the original bark than in the regenerated bark,and significantly higher in the bark of the highly cold tolerant rubber clone PR107 that in that of the less cold tolerant clone Reyan 8-79. This suggests that HbICE2 may be crucial in regulating the cold tolerance of the bark of the rubber tree.
2017, 8(2): 141-146.
doi: 10.15886/j.cnki.rdswxb.2017.02.002
Abstract:
HbNAC1 has been identified as a key transcriptional factor related with laticifer differentiation induced by local dehydration in Hevea brasiliensis. Moreover,HbNAC1 could bind the promoter of and regulate the gene expression of Hb SRPP,suggesting that HbNAC1 might be involved in latex biosynthesis of Hevea brasiliensis. Several cis-elements were inserted into p Ab Ai vector of Yeast-one-hybrid（ Y1H）,and a vector p GADT7-Rec2-HbNAC1 was then constructed for HbNAC1. Both vectors were co-transferred into Y1 H Gold yeast strain to select cis-elements interacted with HbNAC1. Y1 H hybridization results showed that HbNAC1 were bound to ciselements JRE,CACG and ABRE and activated the expression of AUR1-C report gene. These results are very important for identification and characterization of target genes in downstream in the future.
HbNAC1 has been identified as a key transcriptional factor related with laticifer differentiation induced by local dehydration in Hevea brasiliensis. Moreover,HbNAC1 could bind the promoter of and regulate the gene expression of Hb SRPP,suggesting that HbNAC1 might be involved in latex biosynthesis of Hevea brasiliensis. Several cis-elements were inserted into p Ab Ai vector of Yeast-one-hybrid（ Y1H）,and a vector p GADT7-Rec2-HbNAC1 was then constructed for HbNAC1. Both vectors were co-transferred into Y1 H Gold yeast strain to select cis-elements interacted with HbNAC1. Y1 H hybridization results showed that HbNAC1 were bound to ciselements JRE,CACG and ABRE and activated the expression of AUR1-C report gene. These results are very important for identification and characterization of target genes in downstream in the future.
2017, 8(2): 147-152,167.
doi: 10.15886/j.cnki.rdswxb.2017.02.003
Abstract:
HO-73 strain of Oidium heveae B. A. Steinmann was cultured on parafilm surface at different hours post inoculation（ hpi） under different temperatures to observe its infection process and the infection structure formation of its conidia. O. heveae was cultured at the temperatures ranging from 15 ℃ to 34 ℃ and the suitable temperatures for conidium germination and appressorium formation ranged from 22 ℃ to 28 ℃ with the optimal temperature being 22 ℃. The infection process of the conidia of the strain from 0-24 hpi was divided into 4 stages: no germination at 0 hpi,germination at 4 hpi,appressorium formation at 6,8 hpi and secondary appressorium formation at16,24 hpi. Total RNA of the strain was isolated from the cultures at different infection stages from 0 hpi to 24 hpi.The 18 Sr RNA was detected by RT-PCR at all the infection stages and analyzed by using the phylogenetic relationship. Total RNAs of the conidia at the four infection stages were isolated by using the modified Trizol method. The18 S rRNA of O. heveae was detected in all the conidia by RT-PCR,and clustered to the same branch as Erysiphaceae by using phylogenetic analysis. The result indicated that the infection structures of O. heveae were successfully developed on parafilm surface at a temperatures ranging from 22 ℃ to 28 ℃,and that the total RNA extracted from the cultures at 0,4,6,8,16 and 24 hpi can be used for 18 S amplification.
HO-73 strain of Oidium heveae B. A. Steinmann was cultured on parafilm surface at different hours post inoculation（ hpi） under different temperatures to observe its infection process and the infection structure formation of its conidia. O. heveae was cultured at the temperatures ranging from 15 ℃ to 34 ℃ and the suitable temperatures for conidium germination and appressorium formation ranged from 22 ℃ to 28 ℃ with the optimal temperature being 22 ℃. The infection process of the conidia of the strain from 0-24 hpi was divided into 4 stages: no germination at 0 hpi,germination at 4 hpi,appressorium formation at 6,8 hpi and secondary appressorium formation at16,24 hpi. Total RNA of the strain was isolated from the cultures at different infection stages from 0 hpi to 24 hpi.The 18 Sr RNA was detected by RT-PCR at all the infection stages and analyzed by using the phylogenetic relationship. Total RNAs of the conidia at the four infection stages were isolated by using the modified Trizol method. The18 S rRNA of O. heveae was detected in all the conidia by RT-PCR,and clustered to the same branch as Erysiphaceae by using phylogenetic analysis. The result indicated that the infection structures of O. heveae were successfully developed on parafilm surface at a temperatures ranging from 22 ℃ to 28 ℃,and that the total RNA extracted from the cultures at 0,4,6,8,16 and 24 hpi can be used for 18 S amplification.
2017, 8(2): 153-158.
doi: 10.15886/j.cnki.rdswxb.2017.02.004
Abstract:
Transcriptome sequencing of red-flesh and white-flesh pitaya fruit was conducted,and the data of the sequences were analyzed,from which 30 MYB transcription factors differentially expressed were selected. The MYB transcription factors were analyzed by using bioinformatics such as alignment,gene annotation analysis,phylogenetic analysis,gene expression analysis,etc. The bioinformatic analysis was verified by qRT-PCR to explore the regulatory relationships between metabolism difference and MYB transcription factors of white-or redflesh pitaya fruit. The results showed that the differences in pitaya flesh color may be regulated by some members of the MYB family of transcription factors.
Transcriptome sequencing of red-flesh and white-flesh pitaya fruit was conducted,and the data of the sequences were analyzed,from which 30 MYB transcription factors differentially expressed were selected. The MYB transcription factors were analyzed by using bioinformatics such as alignment,gene annotation analysis,phylogenetic analysis,gene expression analysis,etc. The bioinformatic analysis was verified by qRT-PCR to explore the regulatory relationships between metabolism difference and MYB transcription factors of white-or redflesh pitaya fruit. The results showed that the differences in pitaya flesh color may be regulated by some members of the MYB family of transcription factors.
2017, 8(2): 159-164.
doi: 10.15886/j.cnki.rdswxb.2017.02.005
Abstract:
Red-fleshed pitaya（ Hylocerus polyrhizus） fruit were coated with three waxes（ SP-1,Gaoliang and Lizhangjing） to observe their weight loss rate and active oxygen metabolism. The results showed that wax coatings significantly inhibited the increase of weight loss rate and delayed the decline of total soluble solids（ TSS）content,and hence were beneficial to fruit quality. Wax coatings significantly reduced the lipoxygenase（ LOX）activities of the pitaya fruit at the mid stage of storage（ at days 4-6 after storage）,highly significantly increased the ascorbate peroxidase（ APX） activities at the storage stage and the activities of peroxidase（ POD） and catalase（ CAT） at the late storage stage,and highly significantly inhibited the decrease of superoxide dismutase（ SOD） activities,the accumulation of H2O2 contents and the production rate of O2-.at the first 6 days of storage. The three waxes had different effects on the weight loss rate,active oxygen production and the activities of protective enzyme of the pitaya fruit. Of the three waxes the SP-1 wax had the best effect on the fruit quality,followed by ‘Gaoliang’wax.
Red-fleshed pitaya（ Hylocerus polyrhizus） fruit were coated with three waxes（ SP-1,Gaoliang and Lizhangjing） to observe their weight loss rate and active oxygen metabolism. The results showed that wax coatings significantly inhibited the increase of weight loss rate and delayed the decline of total soluble solids（ TSS）content,and hence were beneficial to fruit quality. Wax coatings significantly reduced the lipoxygenase（ LOX）activities of the pitaya fruit at the mid stage of storage（ at days 4-6 after storage）,highly significantly increased the ascorbate peroxidase（ APX） activities at the storage stage and the activities of peroxidase（ POD） and catalase（ CAT） at the late storage stage,and highly significantly inhibited the decrease of superoxide dismutase（ SOD） activities,the accumulation of H2O2 contents and the production rate of O2-.at the first 6 days of storage. The three waxes had different effects on the weight loss rate,active oxygen production and the activities of protective enzyme of the pitaya fruit. Of the three waxes the SP-1 wax had the best effect on the fruit quality,followed by ‘Gaoliang’wax.
2017, 8(2): 165-168.
doi: 10.15886/j.cnki.rdswxb.2017.02.006
Abstract:
A survey of plant resources was made in September 2016 in the Wuzhishan National Nature Reserve to inventory the plant resources and biodiversity of the National Nature Reserve. In this survey the genus Petrosavia Becc.,represented by Petrosavia sakuraii（ Makino） J. J. Sm. ex Steenis and two ferns,namely Asplenium×xinyiense Ching ＆ S. H. Wu（ Aspleniaceae） Metathelypteris uraiensis（ Rosenst.） Ching（ Thelypteridaceae）,were recorded for the first time in Hainan Island,Hainan Province,China.
A survey of plant resources was made in September 2016 in the Wuzhishan National Nature Reserve to inventory the plant resources and biodiversity of the National Nature Reserve. In this survey the genus Petrosavia Becc.,represented by Petrosavia sakuraii（ Makino） J. J. Sm. ex Steenis and two ferns,namely Asplenium×xinyiense Ching ＆ S. H. Wu（ Aspleniaceae） Metathelypteris uraiensis（ Rosenst.） Ching（ Thelypteridaceae）,were recorded for the first time in Hainan Island,Hainan Province,China.
2017, 8(2): 169-173.
doi: 10.15886/j.cnki.rdswxb.2017.02.007
Abstract:
OBJECTIVE To research the change of volatile oil and patchoulol content of continuous cropping Pogostemon cablin. METHODS In this paper,the dynamic accumulation mode of patchoulol and volatile oil content change during the development of P. cablin after continuous cropping（ TR） and normal growth（ CK） P. cablin were tested by GC-MS. RESULTS The essential oil contents in the two different cropping treatments showed growing trends from 90 to 210 days after cultivation（ DAC） and reached their maximum levels at 210 DAC. The essential oil content in the TR group patchouli significantly lower than the CK group patchouli from 150 to 210 DAC. The patchoulol contents of TR group patchouli oil and CK group patchouli oil have the similar accumulation trends,showed growing trends from 90 to 210 DAC and reached their maximum levels at 210 DAC. The patchoulol contents in the TR group patchouli oil significantly lower than the CK group patchouli oil,during its different growth periods. CONDLUSIONS The contents of volatile oil and patchouli alcohol of continuous cropping of P. cablin decreased significantly. To provide reference data for further research.
OBJECTIVE To research the change of volatile oil and patchoulol content of continuous cropping Pogostemon cablin. METHODS In this paper,the dynamic accumulation mode of patchoulol and volatile oil content change during the development of P. cablin after continuous cropping（ TR） and normal growth（ CK） P. cablin were tested by GC-MS. RESULTS The essential oil contents in the two different cropping treatments showed growing trends from 90 to 210 days after cultivation（ DAC） and reached their maximum levels at 210 DAC. The essential oil content in the TR group patchouli significantly lower than the CK group patchouli from 150 to 210 DAC. The patchoulol contents of TR group patchouli oil and CK group patchouli oil have the similar accumulation trends,showed growing trends from 90 to 210 DAC and reached their maximum levels at 210 DAC. The patchoulol contents in the TR group patchouli oil significantly lower than the CK group patchouli oil,during its different growth periods. CONDLUSIONS The contents of volatile oil and patchouli alcohol of continuous cropping of P. cablin decreased significantly. To provide reference data for further research.
2017, 8(2): 174-177.
doi: 10.15886/j.cnki.rdswxb.2017.02.008
Abstract:
Plukenetia volubilis seeds were collected from a Bawangling planting base,Changjiang county,Hainan province to determine their contents of water,proteins,oils and fatty acids. The results showed that the P.volubilis seeds contained 7. 49% of water and 27. 41% of protein and gave an oil yield of 60. 04% by average,which indicated that P. volubilis seeds were of high protein and oil. Five fatty acids including palmitic acid,stearic acid,oleic acid,linoleic acid and linolenic acid were identified in the P. volubilis seeds by using the gas chromatography system,The seeds contained 90. 16% of unsaturated fatty acids（ oleic acid,linoleic acid and linolenic acid）,and 79. 51% of polyunsaturated fatty acids（ linoleic acid and linolenic acid） which are essential for human body. The findings indicated that P. volubilis could be used as an important resource of liana oil plant.
Plukenetia volubilis seeds were collected from a Bawangling planting base,Changjiang county,Hainan province to determine their contents of water,proteins,oils and fatty acids. The results showed that the P.volubilis seeds contained 7. 49% of water and 27. 41% of protein and gave an oil yield of 60. 04% by average,which indicated that P. volubilis seeds were of high protein and oil. Five fatty acids including palmitic acid,stearic acid,oleic acid,linoleic acid and linolenic acid were identified in the P. volubilis seeds by using the gas chromatography system,The seeds contained 90. 16% of unsaturated fatty acids（ oleic acid,linoleic acid and linolenic acid）,and 79. 51% of polyunsaturated fatty acids（ linoleic acid and linolenic acid） which are essential for human body. The findings indicated that P. volubilis could be used as an important resource of liana oil plant.
2017, 8(2): 178-184.
doi: 10.15886/j.cnki.rdswxb.2017.02.009
Abstract:
Mango fruits of mango variety ‘Hongguifei’with 80% maturity were collected from the mango trees aged 15 years old,and spray treated with gibberellic acid（ GA3） solution at different concentrations of 0,0. 3,0. 5,1. 0,2. 0,3. 0 g·L-1to observe their change in fruit color and its related enzyme activities.. The results showed that with storage,the mango fruits treated with GA3 at different concentrations increased gradually in decay index,color a*value,carotenoid content,anthocyanin content,and activities of chlorophyll enzyme,phenylalanine ammonia lyase（ PAL） and chalcone isomerase（ CHI）,and declined gradually in the content of chlorophyll,and the fruit peel changed from dark to light green,and gradually to red or yellow. Compared to the control the treatment of GA3 inhibited the change of color in the fruit peel at the concentrations of 0. 3,0. 5 or 1. 0 g·L-1and evidently at 2. 0 or 3. 0 g·L-1,but the latter had poor impact on the fruit appearance and flavor of the ripening mango fruit. Considering the overall effect of the treatments and the fruit appearance it is better to treat the mango fruits with GA3 at a concentration of 1 g·L-1. According to the preservation effect and exterior quality,1. 0 g·L-1GA3 solution was the appropriate for mango fruits.
Mango fruits of mango variety ‘Hongguifei’with 80% maturity were collected from the mango trees aged 15 years old,and spray treated with gibberellic acid（ GA3） solution at different concentrations of 0,0. 3,0. 5,1. 0,2. 0,3. 0 g·L-1to observe their change in fruit color and its related enzyme activities.. The results showed that with storage,the mango fruits treated with GA3 at different concentrations increased gradually in decay index,color a*value,carotenoid content,anthocyanin content,and activities of chlorophyll enzyme,phenylalanine ammonia lyase（ PAL） and chalcone isomerase（ CHI）,and declined gradually in the content of chlorophyll,and the fruit peel changed from dark to light green,and gradually to red or yellow. Compared to the control the treatment of GA3 inhibited the change of color in the fruit peel at the concentrations of 0. 3,0. 5 or 1. 0 g·L-1and evidently at 2. 0 or 3. 0 g·L-1,but the latter had poor impact on the fruit appearance and flavor of the ripening mango fruit. Considering the overall effect of the treatments and the fruit appearance it is better to treat the mango fruits with GA3 at a concentration of 1 g·L-1. According to the preservation effect and exterior quality,1. 0 g·L-1GA3 solution was the appropriate for mango fruits.
2017, 8(2): 185-189.
doi: 10.15886/j.cnki.rdswxb.2017.02.010
Abstract:
In order to understand the biocontrol potential of entomopathogenic nematode Heterorhabditis bacteriophora SD（ Hb SD） on Meloidogyne incognita,we inoculated Hb SD infected juveniles（ IJs） suspension and the2 nd stage juveniles（ J2） of M. incognita onto potted tomatoes to test the changes of oocyst numbers on tomato roots,root knot index（ RKI） and reproduction factor（ Rf） to study the suppression effect of Hb SD on M. incognita. The results showed that Hb SD inoculation at the 3 different inoculation time and 2 concentrations had a significantly different（ P ﹤ 0. 05） effect on the oocyst numbers,RKI and Rf. The RKI,the oocyst number and the Rf of the treatment Hb SD 6 000 IJs + M. incognita were 2. 67,53. 8 and 19. 77 in the tomato roots,which were obviously lower than those of the control（ 3. 5,82. 2 and 26. 81） and inhibited upto 31. 40% of M. incognita in the tomato roots.
In order to understand the biocontrol potential of entomopathogenic nematode Heterorhabditis bacteriophora SD（ Hb SD） on Meloidogyne incognita,we inoculated Hb SD infected juveniles（ IJs） suspension and the2 nd stage juveniles（ J2） of M. incognita onto potted tomatoes to test the changes of oocyst numbers on tomato roots,root knot index（ RKI） and reproduction factor（ Rf） to study the suppression effect of Hb SD on M. incognita. The results showed that Hb SD inoculation at the 3 different inoculation time and 2 concentrations had a significantly different（ P ﹤ 0. 05） effect on the oocyst numbers,RKI and Rf. The RKI,the oocyst number and the Rf of the treatment Hb SD 6 000 IJs + M. incognita were 2. 67,53. 8 and 19. 77 in the tomato roots,which were obviously lower than those of the control（ 3. 5,82. 2 and 26. 81） and inhibited upto 31. 40% of M. incognita in the tomato roots.
2017, 8(2): 190-195,224.
doi: 10.15886/j.cnki.rdswxb.2017.02.011
Abstract:
Wax apple（ Syzygium samarangense） fruits were dipped or fumigated with tea tree oil at different concentrations to investigate the effects of tea tree oil treatments on postharvest fruit quality of wax apple during storage,and the inhibition effect of tea tree oil on the postharvest pathogenic fungi infecting wax apple. The inhibition effects of the treatments were observed according to the mycelial growth rate of the pathogenic fungi by using the filter paper diffusion method. The results showed that tea tree oil treatments reduced the fruit decay index,and that the treated fruits had higher firmness and higher contents of soluble solids,titratable acid and vitamin C under storage. The treatments significantly improved the postharvest quality of wax apple during storage,Dipping treatment with 10% or fumigation treatment with 500 μL·L-1of tea tree oil had the best effects on the postharvest quality of the wax apple fruits. The result also proved the tea tree oil can significantly inhibit the growth of the pathogenic fungi of Pestalotiopsis guepinii and Phytophthora palmivora infecting wax apple fruit.
Wax apple（ Syzygium samarangense） fruits were dipped or fumigated with tea tree oil at different concentrations to investigate the effects of tea tree oil treatments on postharvest fruit quality of wax apple during storage,and the inhibition effect of tea tree oil on the postharvest pathogenic fungi infecting wax apple. The inhibition effects of the treatments were observed according to the mycelial growth rate of the pathogenic fungi by using the filter paper diffusion method. The results showed that tea tree oil treatments reduced the fruit decay index,and that the treated fruits had higher firmness and higher contents of soluble solids,titratable acid and vitamin C under storage. The treatments significantly improved the postharvest quality of wax apple during storage,Dipping treatment with 10% or fumigation treatment with 500 μL·L-1of tea tree oil had the best effects on the postharvest quality of the wax apple fruits. The result also proved the tea tree oil can significantly inhibit the growth of the pathogenic fungi of Pestalotiopsis guepinii and Phytophthora palmivora infecting wax apple fruit.
2017, 8(2): 196-202.
doi: 10.15886/j.cnki.rdswxb.2017.02.012
Abstract:
An indoor experiment was conducted to investigate the effects of bagasse and its biochar on greenhouse gas emissions from the paddy soil under 30 ℃ and waterlogged conditions. Three treatments were arranged: bagasse,biochar from bagasse and control（ CK）. The results showed that the bagasse-based biochar significantly increased contents of organic carbon,total N,available P and K,whereas bagasse significantly increased soil organic carbon content. Both biochar and bagasse mitigated N2O emissions but were different in the mechanism of N2O emission mitigation due to the difference in the effect of bagasse and its biochar on soil redox potential（ Eh）. The bagasse consumed mainly oxygen to form a strong reduction in the soil,which was not conducive to the production of N2O,while the bagasse-based biochar mitigated the emission of N2O through soil aeration suitable for nitrification. Both bagasse and bagasse-based biochar did not create a soil Eh environment suitable for CH4 emission and had no change in CH4 emissions. There was no significant difference in overall greenhouse effect between bagasse and its biochar,but they had significantly lower greenhouse effect than CK. It was recommended to convert bagasse into biochar for application in the paddy field. Bagasse-based biochar can not only improve the soil but also mitigate the emissions of greenhouse gases.
An indoor experiment was conducted to investigate the effects of bagasse and its biochar on greenhouse gas emissions from the paddy soil under 30 ℃ and waterlogged conditions. Three treatments were arranged: bagasse,biochar from bagasse and control（ CK）. The results showed that the bagasse-based biochar significantly increased contents of organic carbon,total N,available P and K,whereas bagasse significantly increased soil organic carbon content. Both biochar and bagasse mitigated N2O emissions but were different in the mechanism of N2O emission mitigation due to the difference in the effect of bagasse and its biochar on soil redox potential（ Eh）. The bagasse consumed mainly oxygen to form a strong reduction in the soil,which was not conducive to the production of N2O,while the bagasse-based biochar mitigated the emission of N2O through soil aeration suitable for nitrification. Both bagasse and bagasse-based biochar did not create a soil Eh environment suitable for CH4 emission and had no change in CH4 emissions. There was no significant difference in overall greenhouse effect between bagasse and its biochar,but they had significantly lower greenhouse effect than CK. It was recommended to convert bagasse into biochar for application in the paddy field. Bagasse-based biochar can not only improve the soil but also mitigate the emissions of greenhouse gases.
2017, 8(2): 203-208.
doi: 10.15886/j.cnki.rdswxb.2017.02.013
Abstract:
To understand the effect of different molybdenum concentrations on interaction of Stylosanthes guianensis ‘Reyan No. 2’with different rhizobium symbiotic system,two-factor randomized block design were arranged in this experiment,and the Stylosanthes seedlings inoculated with three rhizobium strains were planted in pots filled with sand containing seven different molybdenum concentrations. They were determined in terms of dry weight,plant height,plant nitrogen content,nodule numbers and nitrogenase activity to evaluate the effect of molybdenum on the syntaxial system by principal components analysis. The results showed that molybdenum had little effect on nodule numbers of S. guianensis ‘Reyan No. 2’inoculated with different rhizobium strains,but on the plant height and fresh weight mainly due to the effect of molybdenum on nitrogenase activity. The optimal molybdenum concentrations for different rhizobium strains were different. The optimal molybdenum concentrations for RJS9-2,YM11-1 and PN13-3 were 1 000-1 500 mg·L-1,1 000-2 000 mg·L-1and 2 000-2 500mg·L-1,respectively.
To understand the effect of different molybdenum concentrations on interaction of Stylosanthes guianensis ‘Reyan No. 2’with different rhizobium symbiotic system,two-factor randomized block design were arranged in this experiment,and the Stylosanthes seedlings inoculated with three rhizobium strains were planted in pots filled with sand containing seven different molybdenum concentrations. They were determined in terms of dry weight,plant height,plant nitrogen content,nodule numbers and nitrogenase activity to evaluate the effect of molybdenum on the syntaxial system by principal components analysis. The results showed that molybdenum had little effect on nodule numbers of S. guianensis ‘Reyan No. 2’inoculated with different rhizobium strains,but on the plant height and fresh weight mainly due to the effect of molybdenum on nitrogenase activity. The optimal molybdenum concentrations for different rhizobium strains were different. The optimal molybdenum concentrations for RJS9-2,YM11-1 and PN13-3 were 1 000-1 500 mg·L-1,1 000-2 000 mg·L-1and 2 000-2 500mg·L-1,respectively.
2017, 8(2): 209-215.
doi: 10.15886/j.cnki.rdswxb.2017.02.014
Abstract:
Biogas slurry was used to prepare a formulated manure to apply to the banana to determine its effect on yield,fruit quality and soil properties of banana in Hainan. The results showed that the biogas slurry formulated manure improved the yield and fruit quality of banana,the laterite soil,the p H of acid soil,and the soil nutrient content. Compared with the conventional fertilizers,this formulated manure increased the fruit length by 5. 59%,the finger girth by 11. 01%,the number of hands by 3. 21%,the yield per plant by 4. 09%,the protein content of banana fruit by 10. 67%,the soluble sugar content by 6. 66%,the vitamin C by 3. 32%,the soil organic matter content by 2. 98%-3. 93%,the alkaline hydrolizable nitrogen content by 2. 25%-17. 07%,the available phosphorus by 5. 59%-18. 64%,and the available potassium by 25. 20%-39. 20%. The formulated manure improved not only the crop yield and fruit quality,but also the soil regime of the banana plantation. This study provides reference for further development and promotion of biogas slurry manure and its use in improving soil properties in Hainan.
Biogas slurry was used to prepare a formulated manure to apply to the banana to determine its effect on yield,fruit quality and soil properties of banana in Hainan. The results showed that the biogas slurry formulated manure improved the yield and fruit quality of banana,the laterite soil,the p H of acid soil,and the soil nutrient content. Compared with the conventional fertilizers,this formulated manure increased the fruit length by 5. 59%,the finger girth by 11. 01%,the number of hands by 3. 21%,the yield per plant by 4. 09%,the protein content of banana fruit by 10. 67%,the soluble sugar content by 6. 66%,the vitamin C by 3. 32%,the soil organic matter content by 2. 98%-3. 93%,the alkaline hydrolizable nitrogen content by 2. 25%-17. 07%,the available phosphorus by 5. 59%-18. 64%,and the available potassium by 25. 20%-39. 20%. The formulated manure improved not only the crop yield and fruit quality,but also the soil regime of the banana plantation. This study provides reference for further development and promotion of biogas slurry manure and its use in improving soil properties in Hainan.
2017, 8(2): 216-219.
doi: 10.15886/j.cnki.rdswxb.2017.02.015
Abstract:
In order to provide a convenient and efficient guidance service for control of konjac soft rot an expert system for control of konjac soft rot was designed and developed based on Android system with GPS in Android smartphone. The digital information of a place where a farmer who had a problem of konjac soft rot in his/her konjac plantations was located,such as latitude and longitude,was collected and analyzed by the system,and the system gave the farmer a professional answer to the problem raised by the farmer and recommended the farmer best methods for control control of the disease.
In order to provide a convenient and efficient guidance service for control of konjac soft rot an expert system for control of konjac soft rot was designed and developed based on Android system with GPS in Android smartphone. The digital information of a place where a farmer who had a problem of konjac soft rot in his/her konjac plantations was located,such as latitude and longitude,was collected and analyzed by the system,and the system gave the farmer a professional answer to the problem raised by the farmer and recommended the farmer best methods for control control of the disease.
2017, 8(2): 220-224.
doi: 10.15886/j.cnki.rdswxb.2017.02.016
Abstract:
Scutellaria baicalensis georgi was extracted with distilled water at different ratio to analyze their inhibitory effects on Staphylococcus aureus. The extraction methods were optimized by using orthogonal array design,and the minimum inhibitory concentration（ MIC） and minimum bactericidal concentration（ MBC） of aqueous extracts of S. baicalensis georgi. was analyzed based on inhibitory zone size of the bacteria by using paper disc diffusion method. The results showed that S. baicalensis georgi extracted with distilled water at a ratio of 1∶ 60 for 1 hour at the temperature of 80 ℃ had the best inhibition effect on S. aureus,with its MIC and MBC being 6. 25 g·L-1and25 g·L-1,respectively.
Scutellaria baicalensis georgi was extracted with distilled water at different ratio to analyze their inhibitory effects on Staphylococcus aureus. The extraction methods were optimized by using orthogonal array design,and the minimum inhibitory concentration（ MIC） and minimum bactericidal concentration（ MBC） of aqueous extracts of S. baicalensis georgi. was analyzed based on inhibitory zone size of the bacteria by using paper disc diffusion method. The results showed that S. baicalensis georgi extracted with distilled water at a ratio of 1∶ 60 for 1 hour at the temperature of 80 ℃ had the best inhibition effect on S. aureus,with its MIC and MBC being 6. 25 g·L-1and25 g·L-1,respectively.
2017, 8(2): 225-231.
doi: 10.15886/j.cnki.rdswxb.2017.02.017
Abstract:
Availability of high-quality protein is a prerequisite for proteomic research. Hard bones are major structures for corals,leading to certain interference in extracting of total protein of the corals. Therefore,it is necessary to choose an optimum extraction method for proteomic research in corals. Two species of corals Acropora nasuta and Seriatopora hystrix were extracted by using two extraction methods,TRIzol reagent extraction method and BP-phenol extraction method. The result showed that the protein yield of the extract by BP-phenol extraction was relatively higher. Analysis of the 1-DE and 2-DE electrophoresis showed that the protein samples of the extracts by the two extraction methods were relatively different. The BP-phenol extraction method produced more protein bands and spots with few tailings. The TRIzol reagent method had an advantage of extraction of low molecular weight while the BP-phenol extraction method had an obvious advantage of the extraction of large molecular weight. This result is expected to provide a certain foundation for proteomic studies of stony corals.
Availability of high-quality protein is a prerequisite for proteomic research. Hard bones are major structures for corals,leading to certain interference in extracting of total protein of the corals. Therefore,it is necessary to choose an optimum extraction method for proteomic research in corals. Two species of corals Acropora nasuta and Seriatopora hystrix were extracted by using two extraction methods,TRIzol reagent extraction method and BP-phenol extraction method. The result showed that the protein yield of the extract by BP-phenol extraction was relatively higher. Analysis of the 1-DE and 2-DE electrophoresis showed that the protein samples of the extracts by the two extraction methods were relatively different. The BP-phenol extraction method produced more protein bands and spots with few tailings. The TRIzol reagent method had an advantage of extraction of low molecular weight while the BP-phenol extraction method had an obvious advantage of the extraction of large molecular weight. This result is expected to provide a certain foundation for proteomic studies of stony corals.
2017, 8(2): 232-235.
doi: 10.15886/j.cnki.rdswxb.2017.02.018
Abstract:
Molecular biologicresearch on bacteriophages is primarily based on genomic DNA extraction. The traditional DNA extraction for bacteriophages is complicated in operation andthe use of phenol and chloroform in the DNA extraction is harmful to operator. A new rapid method for bacteriophage genome DNA extraction was developed. Procedures in the method includes nucleic acid degradation of host strains,bacteriophage precipitation,bacteriophage DNA release,impurity removal DNA adsorption,DNA washing,and DNA elution. The results indicated that bacteriophagegenomic DNAs,the host strains of which are Vibrio alginolyticus,Escherichia coli and Enterobacter cloacae,respectively,were extractedin two hours at the soonest,with the yield and purity of genomic DNAsbeing up to 100 ng·μL-1and 1. 8( OD260/280),respectively. This extraction method can prevent the DNA contamination from the host strains,and thus the extractedgenomic DNA of bacteriophagescan be used in further experiments.
Molecular biologicresearch on bacteriophages is primarily based on genomic DNA extraction. The traditional DNA extraction for bacteriophages is complicated in operation andthe use of phenol and chloroform in the DNA extraction is harmful to operator. A new rapid method for bacteriophage genome DNA extraction was developed. Procedures in the method includes nucleic acid degradation of host strains,bacteriophage precipitation,bacteriophage DNA release,impurity removal DNA adsorption,DNA washing,and DNA elution. The results indicated that bacteriophagegenomic DNAs,the host strains of which are Vibrio alginolyticus,Escherichia coli and Enterobacter cloacae,respectively,were extractedin two hours at the soonest,with the yield and purity of genomic DNAsbeing up to 100 ng·μL-1and 1. 8( OD260/280),respectively. This extraction method can prevent the DNA contamination from the host strains,and thus the extractedgenomic DNA of bacteriophagescan be used in further experiments.
2017, 8(2): 236-240.
doi: 10.15886/j.cnki.rdswxb.2017.02.019
Abstract:
Quorum sensing（ QS） is a responding phenomenon of communication between bacteria,providing a new way to study treatment of bacterial infections. The QS system with autoinducter peptides（ AIPs） as signaling molecules exist in a variety of gram-positive bacteria,and is considered as an important breakthrough in development of new drugs against gram-positive bacteria,such as Staphylococcus aureus. The AIP-based quorum sensing inhibitors（ QSIs） discovered so far include two types of molecule,small and macro. The recent researches in these two types of molecule in AIP based QSIs were reviewed to provide a reference for developing of new antibacterial drugs.
Quorum sensing（ QS） is a responding phenomenon of communication between bacteria,providing a new way to study treatment of bacterial infections. The QS system with autoinducter peptides（ AIPs） as signaling molecules exist in a variety of gram-positive bacteria,and is considered as an important breakthrough in development of new drugs against gram-positive bacteria,such as Staphylococcus aureus. The AIP-based quorum sensing inhibitors（ QSIs） discovered so far include two types of molecule,small and macro. The recent researches in these two types of molecule in AIP based QSIs were reviewed to provide a reference for developing of new antibacterial drugs.
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