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Volume 8 Issue 2
Jun.  2017
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Article Navigation >  Journal of Tropical Biology  > 2017  >  8(2): 232-235

LIU Qiuting, TIAN Yushun, YUN Long, LUO Peng, HU Chaoqun. Development of aRapid Method for Extraction of Genomic DNAs of Bacteriophages[J]. Journal of Tropical Biology, 2017, 8(2): 232-235. doi: 10.15886/j.cnki.rdswxb.2017.02.018
Citation: LIU Qiuting, TIAN Yushun, YUN Long, LUO Peng, HU Chaoqun. Development of aRapid Method for Extraction of Genomic DNAs of Bacteriophages[J]. Journal of Tropical Biology, 2017, 8(2): 232-235. doi: 10.15886/j.cnki.rdswxb.2017.02.018
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Development of aRapid Method for Extraction of Genomic DNAs of Bacteriophages

doi: 10.15886/j.cnki.rdswxb.2017.02.018

  • 1. South China Sea Institute of Oceanology, Chinese Academy of Sciences (CAS)/Key Laboratory of Tropical Marine Bio-resources and Ecology, CAS/Guangdong Key Laboratory of Applied Marine Biology,, Guangzhou, Guangdong 510301, China;

  • 2. University of Chinese Academy of Sciences, Beijing 101408, China

  • Received Date: 2017-01-20
  • Rev Recd Date: 2017-02-25
  • Molecular biologicresearch on bacteriophages is primarily based on genomic DNA extraction. The traditional DNA extraction for bacteriophages is complicated in operation andthe use of phenol and chloroform in the DNA extraction is harmful to operator. A new rapid method for bacteriophage genome DNA extraction was developed. Procedures in the method includes nucleic acid degradation of host strains,bacteriophage precipitation,bacteriophage DNA release,impurity removal DNA adsorption,DNA washing,and DNA elution. The results indicated that bacteriophagegenomic DNAs,the host strains of which are Vibrio alginolyticus,Escherichia coli and Enterobacter cloacae,respectively,were extractedin two hours at the soonest,with the yield and purity of genomic DNAsbeing up to 100 ng·μL-1and 1. 8( OD260/280),respectively. This extraction method can prevent the DNA contamination from the host strains,and thus the extractedgenomic DNA of bacteriophagescan be used in further experiments.
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Development of aRapid Method for Extraction of Genomic DNAs of Bacteriophages

doi: 10.15886/j.cnki.rdswxb.2017.02.018
  • 1. South China Sea Institute of Oceanology, Chinese Academy of Sciences (CAS)/Key Laboratory of Tropical Marine Bio-resources and Ecology, CAS/Guangdong Key Laboratory of Applied Marine Biology,, Guangzhou, Guangdong 510301, China;
  • 2. University of Chinese Academy of Sciences, Beijing 101408, China
  • Received Date: 2017-01-20
  • Rev Recd Date: 2017-02-25

Abstract: Molecular biologicresearch on bacteriophages is primarily based on genomic DNA extraction. The traditional DNA extraction for bacteriophages is complicated in operation andthe use of phenol and chloroform in the DNA extraction is harmful to operator. A new rapid method for bacteriophage genome DNA extraction was developed. Procedures in the method includes nucleic acid degradation of host strains,bacteriophage precipitation,bacteriophage DNA release,impurity removal DNA adsorption,DNA washing,and DNA elution. The results indicated that bacteriophagegenomic DNAs,the host strains of which are Vibrio alginolyticus,Escherichia coli and Enterobacter cloacae,respectively,were extractedin two hours at the soonest,with the yield and purity of genomic DNAsbeing up to 100 ng·μL-1and 1. 8( OD260/280),respectively. This extraction method can prevent the DNA contamination from the host strains,and thus the extractedgenomic DNA of bacteriophagescan be used in further experiments.

LIU Qiuting, TIAN Yushun, YUN Long, LUO Peng, HU Chaoqun. Development of aRapid Method for Extraction of Genomic DNAs of Bacteriophages[J]. Journal of Tropical Biology, 2017, 8(2): 232-235. doi: 10.15886/j.cnki.rdswxb.2017.02.018
Citation: LIU Qiuting, TIAN Yushun, YUN Long, LUO Peng, HU Chaoqun. Development of aRapid Method for Extraction of Genomic DNAs of Bacteriophages[J]. Journal of Tropical Biology, 2017, 8(2): 232-235. doi: 10.15886/j.cnki.rdswxb.2017.02.018
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