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Volume 8 Issue 2
Jun.  2017
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Article Navigation >  Journal of Tropical Biology  > 2017  >  8(2): 141-146

HAO Hui, CAO Yuxin, ZHAI Jinling, HUANG Xi. Screening of Cis-elements Interacted with HbNAC1 of Hevea brasiliensis[J]. Journal of Tropical Biology, 2017, 8(2): 141-146. doi: 10.15886/j.cnki.rdswxb.2017.02.002
Citation: HAO Hui, CAO Yuxin, ZHAI Jinling, HUANG Xi. Screening of Cis-elements Interacted with HbNAC1 of Hevea brasiliensis[J]. Journal of Tropical Biology, 2017, 8(2): 141-146. doi: 10.15886/j.cnki.rdswxb.2017.02.002
shu

Screening of Cis-elements Interacted with HbNAC1 of Hevea brasiliensis

doi: 10.15886/j.cnki.rdswxb.2017.02.002

  • Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China

  • Received Date: 2017-02-27
  • Rev Recd Date: 2017-03-20
  • HbNAC1 has been identified as a key transcriptional factor related with laticifer differentiation induced by local dehydration in Hevea brasiliensis. Moreover,HbNAC1 could bind the promoter of and regulate the gene expression of Hb SRPP,suggesting that HbNAC1 might be involved in latex biosynthesis of Hevea brasiliensis. Several cis-elements were inserted into p Ab Ai vector of Yeast-one-hybrid（ Y1H）,and a vector p GADT7-Rec2-HbNAC1 was then constructed for HbNAC1. Both vectors were co-transferred into Y1 H Gold yeast strain to select cis-elements interacted with HbNAC1. Y1 H hybridization results showed that HbNAC1 were bound to ciselements JRE,CACG and ABRE and activated the expression of AUR1-C report gene. These results are very important for identification and characterization of target genes in downstream in the future.
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Screening of Cis-elements Interacted with HbNAC1 of Hevea brasiliensis

doi: 10.15886/j.cnki.rdswxb.2017.02.002
  • Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
  • Received Date: 2017-02-27
  • Rev Recd Date: 2017-03-20

Abstract: HbNAC1 has been identified as a key transcriptional factor related with laticifer differentiation induced by local dehydration in Hevea brasiliensis. Moreover,HbNAC1 could bind the promoter of and regulate the gene expression of Hb SRPP,suggesting that HbNAC1 might be involved in latex biosynthesis of Hevea brasiliensis. Several cis-elements were inserted into p Ab Ai vector of Yeast-one-hybrid（ Y1H）,and a vector p GADT7-Rec2-HbNAC1 was then constructed for HbNAC1. Both vectors were co-transferred into Y1 H Gold yeast strain to select cis-elements interacted with HbNAC1. Y1 H hybridization results showed that HbNAC1 were bound to ciselements JRE,CACG and ABRE and activated the expression of AUR1-C report gene. These results are very important for identification and characterization of target genes in downstream in the future.

HAO Hui, CAO Yuxin, ZHAI Jinling, HUANG Xi. Screening of Cis-elements Interacted with HbNAC1 of Hevea brasiliensis[J]. Journal of Tropical Biology, 2017, 8(2): 141-146. doi: 10.15886/j.cnki.rdswxb.2017.02.002
Citation: HAO Hui, CAO Yuxin, ZHAI Jinling, HUANG Xi. Screening of Cis-elements Interacted with HbNAC1 of Hevea brasiliensis[J]. Journal of Tropical Biology, 2017, 8(2): 141-146. doi: 10.15886/j.cnki.rdswxb.2017.02.002
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