Expression Analysis of MYB Transcription Factors Related to Flesh Color of Pitaya Fruit

Key words: 
• Pitaya /
• fruit colour /
• transcriptome sequencing /
• MYB transcription factor /
• bioinformatics

Abstract: Transcriptome sequencing of red-flesh and white-flesh pitaya fruit was conducted,and the data of the sequences were analyzed,from which 30 MYB transcription factors differentially expressed were selected. The MYB transcription factors were analyzed by using bioinformatics such as alignment,gene annotation analysis,phylogenetic analysis,gene expression analysis,etc. The bioinformatic analysis was verified by qRT-PCR to explore the regulatory relationships between metabolism difference and MYB transcription factors of white-or redflesh pitaya fruit. The results showed that the differences in pitaya flesh color may be regulated by some members of the MYB family of transcription factors.