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Volume 8 Issue 2
Jun.  2017
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Article Navigation >  Journal of Tropical Biology  > 2017  >  8(2): 225-231

CHENG Huamin, YANG Tinghan, XIANG Nan, ZHAO Hongwei, ZHOU Hailong, DIAO Xiaoping. Comparative Study of Different Methods for Extracting of Total Protein of Corals[J]. Journal of Tropical Biology, 2017, 8(2): 225-231. doi: 10.15886/j.cnki.rdswxb.2017.02.017
Citation: CHENG Huamin, YANG Tinghan, XIANG Nan, ZHAO Hongwei, ZHOU Hailong, DIAO Xiaoping. Comparative Study of Different Methods for Extracting of Total Protein of Corals[J]. Journal of Tropical Biology, 2017, 8(2): 225-231. doi: 10.15886/j.cnki.rdswxb.2017.02.017
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Comparative Study of Different Methods for Extracting of Total Protein of Corals

doi: 10.15886/j.cnki.rdswxb.2017.02.017

  • College of Tropical Agriculture and Forestry/Haikou Key Laboratory of Environment Toxicology, Hainan University, Haikou 570228, China

  • Received Date: 2016-12-23
  • Rev Recd Date: 2017-02-15
  • Availability of high-quality protein is a prerequisite for proteomic research. Hard bones are major structures for corals,leading to certain interference in extracting of total protein of the corals. Therefore,it is necessary to choose an optimum extraction method for proteomic research in corals. Two species of corals Acropora nasuta and Seriatopora hystrix were extracted by using two extraction methods,TRIzol reagent extraction method and BP-phenol extraction method. The result showed that the protein yield of the extract by BP-phenol extraction was relatively higher. Analysis of the 1-DE and 2-DE electrophoresis showed that the protein samples of the extracts by the two extraction methods were relatively different. The BP-phenol extraction method produced more protein bands and spots with few tailings. The TRIzol reagent method had an advantage of extraction of low molecular weight while the BP-phenol extraction method had an obvious advantage of the extraction of large molecular weight. This result is expected to provide a certain foundation for proteomic studies of stony corals.
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Comparative Study of Different Methods for Extracting of Total Protein of Corals

doi: 10.15886/j.cnki.rdswxb.2017.02.017
  • College of Tropical Agriculture and Forestry/Haikou Key Laboratory of Environment Toxicology, Hainan University, Haikou 570228, China
  • Received Date: 2016-12-23
  • Rev Recd Date: 2017-02-15

Abstract: Availability of high-quality protein is a prerequisite for proteomic research. Hard bones are major structures for corals,leading to certain interference in extracting of total protein of the corals. Therefore,it is necessary to choose an optimum extraction method for proteomic research in corals. Two species of corals Acropora nasuta and Seriatopora hystrix were extracted by using two extraction methods,TRIzol reagent extraction method and BP-phenol extraction method. The result showed that the protein yield of the extract by BP-phenol extraction was relatively higher. Analysis of the 1-DE and 2-DE electrophoresis showed that the protein samples of the extracts by the two extraction methods were relatively different. The BP-phenol extraction method produced more protein bands and spots with few tailings. The TRIzol reagent method had an advantage of extraction of low molecular weight while the BP-phenol extraction method had an obvious advantage of the extraction of large molecular weight. This result is expected to provide a certain foundation for proteomic studies of stony corals.

CHENG Huamin, YANG Tinghan, XIANG Nan, ZHAO Hongwei, ZHOU Hailong, DIAO Xiaoping. Comparative Study of Different Methods for Extracting of Total Protein of Corals[J]. Journal of Tropical Biology, 2017, 8(2): 225-231. doi: 10.15886/j.cnki.rdswxb.2017.02.017
Citation: CHENG Huamin, YANG Tinghan, XIANG Nan, ZHAO Hongwei, ZHOU Hailong, DIAO Xiaoping. Comparative Study of Different Methods for Extracting of Total Protein of Corals[J]. Journal of Tropical Biology, 2017, 8(2): 225-231. doi: 10.15886/j.cnki.rdswxb.2017.02.017
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