5种饵料对日本囊对虾早期生长及感染WSSV存活率的影响

王平; 孙成波; 庄健进; 李咏; 李义军; 李婷; 徐安敏

doi:10.15886/j.cnki.rdswxb.2010.04.020

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5种饵料对日本囊对虾早期生长及感染WSSV存活率的影响

王平, 孙成波, 庄健进, 李咏, 李义军, 李婷, 徐安敏

文章导航 > 热带生物学报 > 2010 > 1(4): 371-375

王平, 孙成波, 庄健进, 李咏, 李义军, 李婷, 徐安敏. 5种饵料对日本囊对虾早期生长及感染WSSV存活率的影响[J]. 热带生物学报, 2010, 1(4): 371-375. doi: 10.15886/j.cnki.rdswxb.2010.04.020

引用本文:

WANG Ping, SUN Cheng-bo, ZHUANG Jian-jin, LI Yong, LI Yi-jun, LI Ting, XU An-min. Effects of Five Different Diets on Growth and WSSV Resistance of Marsupenaeus japonicus[J]. Journal of Tropical Biology, 2010, 1(4): 371-375. doi: 10.15886/j.cnki.rdswxb.2010.04.020

Citation:

WANG Ping, SUN Cheng-bo, ZHUANG Jian-jin, LI Yong, LI Yi-jun, LI Ting, XU An-min. Effects of Five Different Diets on Growth and WSSV Resistance of Marsupenaeus japonicus[J]. Journal of Tropical Biology, 2010, 1(4): 371-375. doi: 10.15886/j.cnki.rdswxb.2010.04.020

5种饵料对日本囊对虾早期生长及感染WSSV存活率的影响

doi: 10.15886/j.cnki.rdswxb.2010.04.020

王平^1,2,
孙成波¹,
庄健进¹,
李咏²,
李义军^1,2,
李婷¹,
徐安敏^1,2

1. 广东海洋大学水产学院, 广东湛江 524088;
2. 海南省昌江南疆生物技术有限公司, 海南三亚 572000

基金项目:

科技部农业科技成果转化资金项目(2010GB2E200382)

科技部星火计划重点项目(2010GA800008)

海南省重点科技计划项目(090706)

详细信息

第一作者:
王平（1977－），男，云南宣威人，海南省昌江南疆生物技术公司高级工程师.

中图分类号: S963.14

Effects of Five Different Diets on Growth and WSSV Resistance of Marsupenaeus japonicus

1. Fisheries college of Guangdong Ocean University, Zhanjiang 524088, China;
2. Hainan Changjiang Nanjiang Marine Biotechnology Co., Sanya 572000, China

摘要: 采用人工配合饲料、鱼粉、丰年虫无节幼体、虾片和牡蛎肉5种不同饵料投喂日本囊对虾幼虾,实验周期20d,测量日本囊对虾的体长、体重及计算其成活率。结果表明,5种饵料对日本囊对虾生长的影响差异显著（P ＞ 0.05),其中丰年虫组体长、体重的增长明显优于其他各组,差异达极显著水平（P ＜ 0.01）,牡蛎肉组次之,配合饲料组的体长、体重增长最小,鱼粉组体长、体重增长大于虾片组。人工投喂WSSV感染5种不同饵料投喂的日本囊对虾幼虾,实验周期10d。丰年虫组和鱼粉组的存活率最高,明显高于配合饲料和牡蛎肉两实验组,差异达极显著水平（P ＜ 0.01);丰年虫组和鱼粉组之间存活率无显著差异（P ＞ 0.05),人工配合饵料组和牡蛎肉组差异不显著（P ＞ 0.05)。半定量PCR检测表明,感染前日本囊对虾幼虾均不携带WSSV,感染后全部个体均检测到相应的病毒特征片段。
- 日本囊对虾 /
- 饵料 /
- WSSV /
- 半定量PCR
Abstract: Five diets,which were artificial feed,fish meal,artemia salina nauplii,shrimp flakes and oyster meat respectively,were used to feed M.japonicus,and the body length as well as body weight were measured,experimental period was 20 days. The results indicated that the effects of 5 species of diets on the growth of the M.japonicus were significantly different （P＜0.05）; the increasing of the weight and length of the group which fed with Artemia Salina nauplii were significantly higher than that of other groups （P＜0.01）,and the group which was fed with artificial feed got the slowest increasing in length and weight; the length and weight of the group which was fed with fish meal was higher than that of shrimp flakes; After infected with WSSV,and the experiment time was designed as 10 d,the survival rate of artemia salina nauplii group and the fish meal group were significantly higher than that of other groups （P＜0.01）; there was no significant difference between Artemia Salina nauplii group and the fish meal group（P＞0.05）,there was no significant difference between artificial feed group and the oyster meat group（P＞0.05）. The analysis results of semiquantitive PCR showed that there were no WSSV gene fragments before infection but there were positive signs of infection after the experiment.
- M.japonicus /
- diets /
- WSSV /
- semi-quantitive PCR

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5种饵料对日本囊对虾早期生长及感染WSSV存活率的影响

doi: 10.15886/j.cnki.rdswxb.2010.04.020

1. 广东海洋大学水产学院, 广东湛江 524088;

2. 海南省昌江南疆生物技术有限公司, 海南三亚 572000

基金项目:

科技部农业科技成果转化资金项目(2010GB2E200382)

科技部星火计划重点项目(2010GA800008)

海南省重点科技计划项目(090706)

作者简介:
王平（1977－），男，云南宣威人，海南省昌江南疆生物技术公司高级工程师.

收稿日期: 2010-11-15

中图分类号: S963.14

关键词:

日本囊对虾 /

饵料 /

WSSV /

半定量PCR

摘要: 采用人工配合饲料、鱼粉、丰年虫无节幼体、虾片和牡蛎肉5种不同饵料投喂日本囊对虾幼虾,实验周期20d,测量日本囊对虾的体长、体重及计算其成活率。结果表明,5种饵料对日本囊对虾生长的影响差异显著（P ＞ 0.05),其中丰年虫组体长、体重的增长明显优于其他各组,差异达极显著水平（P ＜ 0.01）,牡蛎肉组次之,配合饲料组的体长、体重增长最小,鱼粉组体长、体重增长大于虾片组。人工投喂WSSV感染5种不同饵料投喂的日本囊对虾幼虾,实验周期10d。丰年虫组和鱼粉组的存活率最高,明显高于配合饲料和牡蛎肉两实验组,差异达极显著水平（P ＜ 0.01);丰年虫组和鱼粉组之间存活率无显著差异（P ＞ 0.05),人工配合饵料组和牡蛎肉组差异不显著（P ＞ 0.05)。半定量PCR检测表明,感染前日本囊对虾幼虾均不携带WSSV,感染后全部个体均检测到相应的病毒特征片段。

English Abstract

Effects of Five Different Diets on Growth and WSSV Resistance of Marsupenaeus japonicus

1. Fisheries college of Guangdong Ocean University, Zhanjiang 524088, China;

2. Hainan Changjiang Nanjiang Marine Biotechnology Co., Sanya 572000, China

Received Date: 2010-11-15

Keywords:

Abstract: Five diets,which were artificial feed,fish meal,artemia salina nauplii,shrimp flakes and oyster meat respectively,were used to feed M.japonicus,and the body length as well as body weight were measured,experimental period was 20 days. The results indicated that the effects of 5 species of diets on the growth of the M.japonicus were significantly different （P＜0.05）; the increasing of the weight and length of the group which fed with Artemia Salina nauplii were significantly higher than that of other groups （P＜0.01）,and the group which was fed with artificial feed got the slowest increasing in length and weight; the length and weight of the group which was fed with fish meal was higher than that of shrimp flakes; After infected with WSSV,and the experiment time was designed as 10 d,the survival rate of artemia salina nauplii group and the fish meal group were significantly higher than that of other groups （P＜0.01）; there was no significant difference between Artemia Salina nauplii group and the fish meal group（P＞0.05）,there was no significant difference between artificial feed group and the oyster meat group（P＞0.05）. The analysis results of semiquantitive PCR showed that there were no WSSV gene fragments before infection but there were positive signs of infection after the experiment.