Keywords: 
• Ligustrum lucidum Ait. /
• RAPD /
• impact factors /
• system optimization

Abstract: Ligustrum lucidum Ait. was used as the experimental material for RAPD-PCR. Some important factors,such as the concentrations of Mg Cl₂,d NTPs,random primers,Taq polymerase and template DNA as well as the annealing temperature and the cycles of amplification,were optimized by single factor experiment in order to establish the optimal RAPD-PCR system and procedure. Stable RAPD-PCR reaction parameters for L. lucidum Ait. were determined and optimized. 2. 5 μL 10×PCR buffer; 3. 0 μL 25 mmol · L^-1Mg Cl₂; 0. 5μL d NTPs,0. 4 μL Taq polymerase,1. 0 μL primers,and 60 ng DNA template were contained in 25 μL reaction solution. The PCR amplification program was to pre-denature at 94 ℃ for 4 min,then denature at 94 ℃ for 30 s,anneal at 38 ℃ for 45 s,extend at 72 ℃ for 2 min,and conduct 40 cycles with the last extension at 72 ℃ for 10 min. The amplified products were stored at 16 ℃. With this optimized RAPD reaction system the DNA of L. lucidum Ait. was amplified to produce rich,clear and repeatable bands.