日本囊对虾<i>ML</i>基因的克隆与表达分析

徐伊桦; 苏永全; 毛勇; 宋晓红; 张曼; 王军; 王春忠; 郑志鹏

doi:10.15886/j.cnki.rdswxb.2015.04.003

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日本囊对虾ML基因的克隆与表达分析

徐伊桦, 苏永全, 毛勇, 宋晓红, 张曼, 王军, 王春忠, 郑志鹏

文章导航 > 热带生物学报 > 2015 > 6(4): 365-373

徐伊桦, 苏永全, 毛勇, 宋晓红, 张曼, 王军, 王春忠, 郑志鹏. 日本囊对虾ML基因的克隆与表达分析[J]. 热带生物学报, 2015, 6(4): 365-373. doi: 10.15886/j.cnki.rdswxb.2015.04.003

引用本文:

XU Yihua, SU Yongquan, MAO Yong, SONG Xiaohong, ZHANG Man, WANG Jun, WANG Chunzhong, ZHENG Zhipeng. Cloning and Expression of a ML Superfamily Protein in Marsupenaeus japonicas[J]. Journal of Tropical Biology, 2015, 6(4): 365-373. doi: 10.15886/j.cnki.rdswxb.2015.04.003

Citation:

XU Yihua, SU Yongquan, MAO Yong, SONG Xiaohong, ZHANG Man, WANG Jun, WANG Chunzhong, ZHENG Zhipeng. Cloning and Expression of a ML Superfamily Protein in Marsupenaeus japonicas[J]. Journal of Tropical Biology, 2015, 6(4): 365-373. doi: 10.15886/j.cnki.rdswxb.2015.04.003

日本囊对虾ML基因的克隆与表达分析

doi: 10.15886/j.cnki.rdswxb.2015.04.003

1. 厦门大学海洋与地球学院, 厦门 361005;
2. 莆田市天然星农业开发有限公司, 福建莆田 351100

基金项目:

国家虾产业技术体系项目(CARS-47)

国家863计划项目(2012AA10A409-03)

南方海洋研究中心项目(14CZY033HJ07)

详细信息

第一作者:
徐伊桦(1991-),女,厦门大学海洋与地球学院2013级硕士研究生.E-mail:biyuanlian@gmail.com

通信作者:
王军(1953-),女,教授,硕士生导师.研究方向:鱼类学,海水鱼虾类遗传育种与种质资源研究.E-mail:junw@xmu.edu.cn

中图分类号: S917.4

Cloning and Expression of a ML Superfamily Protein in Marsupenaeus japonicas

1. College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102;
2. Putian Tianranxing Agricultural Development Co., Ltd, Putian 351100, China

摘要: 本研究克隆了日本囊对虾的1个ML基因——MjML,该基因的cDNA全长614 bp,开放阅读框长453 bp,编码150个氨基酸。MjML的基因组序列为1 678 bp,含有4个外显子3个内含子。MjML具有6个在ML蛋白中相对保守的半胱氨酸残基,可能形成3对二硫键。实时荧光定量PCR结果显示,MjML主要在肝胰腺中表达,其他组织中表达极微量。感染白斑综合症病毒(WSSV)后,MjML在肝胰腺中的表达量先上升后下降,于24 h出现显著上调,并达到最高峰值。在日本囊对虾早期幼体发育过程中,MjML表达量在阶段性的蜕皮变态后有大幅的升高;在仔虾期间,MjML表达量呈阶段上升特征。结果表明,MjML不仅可能与日本囊对虾先天性免疫应答相关,而且与其幼体发育过程中的变态及蜕皮也可能有一定的关联。
- 日本囊对虾 /
- ML蛋白 /
- 白斑综合征病毒 /
- 对虾幼体
Abstract: MD-2-related lipid-recognition（ ML） superfamily proteins have a single ML domain,which can be bound to specific lipids and play important roles in innate immunity,lipid-recognition and metabolism. The cDNA and gene of a ML protein was identified form Marsupenaeus japonicus,and this ML protein was named as MjML. The full length cDNA of the MjML was 614 bp,with a 453 bp open reading frame that encoded 150 amino acid residues. The MjML gene was 1678 bp in length,and consisted of four exons and three introns. The putative MjML protein contained 6 cysteines which could form 3 pairs of disulfide bonds. Real-time quantitative PCR results showed that MjML mRNA was mainly highly expressed in the hepatopancreas,but very low in other examined tissues. After V. parahaemolyticus was challenged with white spot syndrome virus（ WSSV）,the expression of MjML in hepatopancreas increased gradually and then decreased. At 24 h,the MjML was upregulated and its expression reached the peak. During the early larval development of V. parahaemolyticus,especially after the metamorphosis,the MjML expression had a sharp rise. The MjML expression increased step by step at the post-larvae stage. The results showed that the MjML might play a role in the innate immunity of V. parahaemolyticus. And during the early larval development,the expression of MjML mRNA there might be correlated with the molting and metamorphosis in shrimp larval development.
- Marsupenaeus japonicus /
- MD-2-related lipid-recognition superfamily /
- white spot syndrome virus（WSSV） /
- shrimp larvae

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日本囊对虾ML基因的克隆与表达分析

doi: 10.15886/j.cnki.rdswxb.2015.04.003

1. 厦门大学海洋与地球学院, 厦门 361005;

2. 莆田市天然星农业开发有限公司, 福建莆田 351100

基金项目:

国家虾产业技术体系项目(CARS-47)

国家863计划项目(2012AA10A409-03)

南方海洋研究中心项目(14CZY033HJ07)

作者简介:
徐伊桦(1991-),女,厦门大学海洋与地球学院2013级硕士研究生.E-mail:biyuanlian@gmail.com

通讯作者: 王军(1953-),女,教授,硕士生导师.研究方向:鱼类学,海水鱼虾类遗传育种与种质资源研究.E-mail:junw@xmu.edu.cn

收稿日期: 2015-05-15

中图分类号: S917.4

关键词:

日本囊对虾 /

ML蛋白 /

白斑综合征病毒 /

对虾幼体

摘要: 本研究克隆了日本囊对虾的1个ML基因——MjML,该基因的cDNA全长614 bp,开放阅读框长453 bp,编码150个氨基酸。MjML的基因组序列为1 678 bp,含有4个外显子3个内含子。MjML具有6个在ML蛋白中相对保守的半胱氨酸残基,可能形成3对二硫键。实时荧光定量PCR结果显示,MjML主要在肝胰腺中表达,其他组织中表达极微量。感染白斑综合症病毒(WSSV)后,MjML在肝胰腺中的表达量先上升后下降,于24 h出现显著上调,并达到最高峰值。在日本囊对虾早期幼体发育过程中,MjML表达量在阶段性的蜕皮变态后有大幅的升高;在仔虾期间,MjML表达量呈阶段上升特征。结果表明,MjML不仅可能与日本囊对虾先天性免疫应答相关,而且与其幼体发育过程中的变态及蜕皮也可能有一定的关联。

English Abstract

Cloning and Expression of a ML Superfamily Protein in Marsupenaeus japonicas

1. College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102;

2. Putian Tianranxing Agricultural Development Co., Ltd, Putian 351100, China

Received Date: 2015-05-15

Keywords:

Marsupenaeus japonicus /
MD-2-related lipid-recognition superfamily /
white spot syndrome virus（WSSV） /
shrimp larvae

Abstract: MD-2-related lipid-recognition（ ML） superfamily proteins have a single ML domain,which can be bound to specific lipids and play important roles in innate immunity,lipid-recognition and metabolism. The cDNA and gene of a ML protein was identified form Marsupenaeus japonicus,and this ML protein was named as MjML. The full length cDNA of the MjML was 614 bp,with a 453 bp open reading frame that encoded 150 amino acid residues. The MjML gene was 1678 bp in length,and consisted of four exons and three introns. The putative MjML protein contained 6 cysteines which could form 3 pairs of disulfide bonds. Real-time quantitative PCR results showed that MjML mRNA was mainly highly expressed in the hepatopancreas,but very low in other examined tissues. After V. parahaemolyticus was challenged with white spot syndrome virus（ WSSV）,the expression of MjML in hepatopancreas increased gradually and then decreased. At 24 h,the MjML was upregulated and its expression reached the peak. During the early larval development of V. parahaemolyticus,especially after the metamorphosis,the MjML expression had a sharp rise. The MjML expression increased step by step at the post-larvae stage. The results showed that the MjML might play a role in the innate immunity of V. parahaemolyticus. And during the early larval development,the expression of MjML mRNA there might be correlated with the molting and metamorphosis in shrimp larval development.

引用本文:

Citation:

XU Yihua, SU Yongquan, MAO Yong, SONG Xiaohong, ZHANG Man, WANG Jun, WANG Chunzhong, ZHENG Zhipeng. Cloning and Expression of a ML Superfamily Protein in Marsupenaeus japonicas[J]. Journal of Tropical Biology, 2015, 6(4): 365-373. doi: 10.15886/j.cnki.rdswxb.2015.04.003