2011 Vol. 2, No. 4
2011, 2(4): 291-296.
doi: 10.15886/j.cnki.rdswxb.2011.04.010
Abstract
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Abstract:
Cotton sucrose synthase 3 （SUS3） plays an important role during the cotton fiber development. Compared to Arabidopsis SUS1/SUS4, the gene structure of cotton SUS3 has a high similarity with that of those two genes. To determine the function of the first intron of cotton SUS3, the intron was isolated and inserted into the GUS coding region to construct vector Susfig:121, and which was transformed into Arabidopsis thaliana mediated by agrobacterium. The results of GUS activity analysis of transgenic Arabidopsis plants indicated that the intron down-regulated GUS gene expression in non-pollen tissues and specifically repressed GUS expression in pollen, which suggested that the first intron played a role in gene expression.
Cotton sucrose synthase 3 （SUS3） plays an important role during the cotton fiber development. Compared to Arabidopsis SUS1/SUS4, the gene structure of cotton SUS3 has a high similarity with that of those two genes. To determine the function of the first intron of cotton SUS3, the intron was isolated and inserted into the GUS coding region to construct vector Susfig:121, and which was transformed into Arabidopsis thaliana mediated by agrobacterium. The results of GUS activity analysis of transgenic Arabidopsis plants indicated that the intron down-regulated GUS gene expression in non-pollen tissues and specifically repressed GUS expression in pollen, which suggested that the first intron played a role in gene expression.
2011, 2(4): 297-301.
doi: 10.15886/j.cnki.rdswxb.2011.04.001
Abstract:
In the paper, the mechanism of HSPs promoting plant heat resistance, cold resistance and drought resistance were reviewed. And the strategies that the protein-protein interaction analysis techniques such as yeast two-hybridization, Co-Immunoprecipitation, and so on, are performed to screen the target protein and elucidate the effects of interaction were proposed.
In the paper, the mechanism of HSPs promoting plant heat resistance, cold resistance and drought resistance were reviewed. And the strategies that the protein-protein interaction analysis techniques such as yeast two-hybridization, Co-Immunoprecipitation, and so on, are performed to screen the target protein and elucidate the effects of interaction were proposed.
2011, 2(4): 302-304.
doi: 10.15886/j.cnki.rdswxb.2011.04.002
Abstract:
In this report,a species, Strobilanthes bantonensis Lindau, was reported as new recorded species of Acanthaceae from Hainan Island, China,and its morphology and habit were described. The voucher specimens are preserved in Herbarium of Research Institute of Tropical Forestry, Chinese Academy of Forestry （RITF） and South China Agricultural University （CANT） and South China Botanical Garden, Chinese Academy of Sciences （IBSC）.
In this report,a species, Strobilanthes bantonensis Lindau, was reported as new recorded species of Acanthaceae from Hainan Island, China,and its morphology and habit were described. The voucher specimens are preserved in Herbarium of Research Institute of Tropical Forestry, Chinese Academy of Forestry （RITF） and South China Agricultural University （CANT） and South China Botanical Garden, Chinese Academy of Sciences （IBSC）.
2011, 2(4): 305-309,315.
doi: 10.15886/j.cnki.rdswxb.2011.04.020
Abstract:
The species, biomass, infection rate, infection position of biofouling at Pteria penguin and its effect on their growth performances were analyzed at different seasons. The results showed that ① the species and the biomass of fouling organisms on the Pteria penguin varied with seasons significantly, which were significantly higher in summer and autumn than that in spring and winter （that in summer was the highest）（P＜0.05）; the common dominant species of biofouling in summer and autumn were sponge （phylum porifera）, polydora, hydroides, serpula, balanus and sea squirt, however, they were horny coral, balanus and bugula in spring, they were sea squirt, balanus and bugula in winter; in winter, the attachment situation of wild Pteria penguin were similar to that of cultured Pteria penguin, however, the parasitic load of polydora, hydroides and serpula in wild Pteria penguin were significantly lower than that in cultured Pteria penguin. ②the cultured Pteria penguin were infected by polychaete parasitosis in all seasons, and the infection rates of spring, summer, autumn and winter were 11.76%, 36.51%, 27.58%, 19.56%, respectively; in winter, the infection rate of cultured Pteria penguin was higher than that of wild Pteria penguin; the infection rates of surface shell were higher than that of the inner shell as well as both surface and inner shell in all seasons; in spring and winter, the infection rates of the inner shell were higher than that of both surface and inner shell, while in summer and autumn the results were opposite. ③ the growth performances of healthy Pteria penguin were higher than that of the infected one, especially in summer, the difference was significant.
The species, biomass, infection rate, infection position of biofouling at Pteria penguin and its effect on their growth performances were analyzed at different seasons. The results showed that ① the species and the biomass of fouling organisms on the Pteria penguin varied with seasons significantly, which were significantly higher in summer and autumn than that in spring and winter （that in summer was the highest）（P＜0.05）; the common dominant species of biofouling in summer and autumn were sponge （phylum porifera）, polydora, hydroides, serpula, balanus and sea squirt, however, they were horny coral, balanus and bugula in spring, they were sea squirt, balanus and bugula in winter; in winter, the attachment situation of wild Pteria penguin were similar to that of cultured Pteria penguin, however, the parasitic load of polydora, hydroides and serpula in wild Pteria penguin were significantly lower than that in cultured Pteria penguin. ②the cultured Pteria penguin were infected by polychaete parasitosis in all seasons, and the infection rates of spring, summer, autumn and winter were 11.76%, 36.51%, 27.58%, 19.56%, respectively; in winter, the infection rate of cultured Pteria penguin was higher than that of wild Pteria penguin; the infection rates of surface shell were higher than that of the inner shell as well as both surface and inner shell in all seasons; in spring and winter, the infection rates of the inner shell were higher than that of both surface and inner shell, while in summer and autumn the results were opposite. ③ the growth performances of healthy Pteria penguin were higher than that of the infected one, especially in summer, the difference was significant.
2011, 2(4): 310-315.
doi: 10.15886/j.cnki.rdswxb.2011.04.014
Abstract:
In the report, to explore the drought-adaptation mechanism of T.indica, the root growth, water content and proline content in plant tissues, photosynthetic characteristic and transpiration characteristic were analyzed. The results showed that it is under arid environment that the roots of T.indica were greatly proliferated, and which were beneficial for absorbing water from soil to meet the request of physiological water; the bund water content in plant tissue was higher than free water content, which would result in slow hydraulic metabolism which should be helpful to adapt to arid environment; the proline content in plant tissue increased during dry season, and which could facilitate cell water retaining and prevent dehydration; to adapt dry environment, T.indica regulated the photosynthetic rate and transpiration rate by adjusting stomata conductance. However, all these adaptive characters would limit their productivity potential.
In the report, to explore the drought-adaptation mechanism of T.indica, the root growth, water content and proline content in plant tissues, photosynthetic characteristic and transpiration characteristic were analyzed. The results showed that it is under arid environment that the roots of T.indica were greatly proliferated, and which were beneficial for absorbing water from soil to meet the request of physiological water; the bund water content in plant tissue was higher than free water content, which would result in slow hydraulic metabolism which should be helpful to adapt to arid environment; the proline content in plant tissue increased during dry season, and which could facilitate cell water retaining and prevent dehydration; to adapt dry environment, T.indica regulated the photosynthetic rate and transpiration rate by adjusting stomata conductance. However, all these adaptive characters would limit their productivity potential.
2011, 2(4): 316-320.
doi: 10.15886/j.cnki.rdswxb.2011.04.011
Abstract:
In our report, the effects of different planting pattern and planting density on main agronomic characters and pests of Arabica coffee were analyzed. The results suggested that the difference is not significant,but for the length of the longest first branch and the number of the longest first branching node,the smaller the planting density was,the smaller the rate was,the more fruit nodes, the higher yield per plant. The highest yield obtained from the planting pattern with planting spacing of 0.6 m×1.6 m and the tree number of 693 per mu, and under which the yield per mu attained 249.04 kg dry parchment coffee.
In our report, the effects of different planting pattern and planting density on main agronomic characters and pests of Arabica coffee were analyzed. The results suggested that the difference is not significant,but for the length of the longest first branch and the number of the longest first branching node,the smaller the planting density was,the smaller the rate was,the more fruit nodes, the higher yield per plant. The highest yield obtained from the planting pattern with planting spacing of 0.6 m×1.6 m and the tree number of 693 per mu, and under which the yield per mu attained 249.04 kg dry parchment coffee.
2011, 2(4): 321-327.
doi: 10.15886/j.cnki.rdswxb.2011.04.003
Abstract:
In our report, the species diversity of insects in Wugui Mountain Nature Reserve, Zhongshan were investigated, in which survey approach and classification methods were performed as described in Reference 1. Totally, 249 species from 29 families of Coleoptera were included.
In our report, the species diversity of insects in Wugui Mountain Nature Reserve, Zhongshan were investigated, in which survey approach and classification methods were performed as described in Reference 1. Totally, 249 species from 29 families of Coleoptera were included.
2011, 2(4): 328-332.
doi: 10.15886/j.cnki.rdswxb.2011.04.012
Abstract:
In the report, 4 photo-thermo-sensitive genetic male sterile （PTGMS） lines, Peiai64S, ZhunS, P88S and Y58S were analyzed by sowing data treatment in Haikou, and the fertility performance was determined by observing the percentage of pollen fertility and seed-setting on the bagged panicles. The results showed that the fertility of Peiai64S was stable from mid-May to late-October, and the temperature sensitive periods （TSP） was 10—25 d before heading; the fertility of ZhunS was stable from May 15th to Oct. 16th, and the TSP was 5—15 d and 19—22 d before heading; the fertility of P88S was stable from May 11th to Oct. 18th; and the TSP was 5—15 d and 18—22 d before heading; the fertility of Y58S was stable from May 5th to Oct. 20th; and the TSP was 7—11d before heading.
In the report, 4 photo-thermo-sensitive genetic male sterile （PTGMS） lines, Peiai64S, ZhunS, P88S and Y58S were analyzed by sowing data treatment in Haikou, and the fertility performance was determined by observing the percentage of pollen fertility and seed-setting on the bagged panicles. The results showed that the fertility of Peiai64S was stable from mid-May to late-October, and the temperature sensitive periods （TSP） was 10—25 d before heading; the fertility of ZhunS was stable from May 15th to Oct. 16th, and the TSP was 5—15 d and 19—22 d before heading; the fertility of P88S was stable from May 11th to Oct. 18th; and the TSP was 5—15 d and 18—22 d before heading; the fertility of Y58S was stable from May 5th to Oct. 20th; and the TSP was 7—11d before heading.
2011, 2(4): 333-337.
doi: 10.15886/j.cnki.rdswxb.2011.04.015
Abstract:
In the report, the facilities cultivation and field cultivation of four varieties of capsicum were performed and the differences about the 33 qualitative characteristics and pseudo-quality characteristics and 11 quantitative characteristics were determined. The results indicated that there were no significant differences on the quality characteristic between the facilities cultivation and field cultivation, however, there were significant differences on the quantity characteristics,especially for the characteristics about the leaf length and leaf width, between the facilities cultivation and field cultivation. So, in winter, considering the types and characteristics was necessary, and in order to utilize the data obtained, DUS test based on testing types should be performed.
In the report, the facilities cultivation and field cultivation of four varieties of capsicum were performed and the differences about the 33 qualitative characteristics and pseudo-quality characteristics and 11 quantitative characteristics were determined. The results indicated that there were no significant differences on the quality characteristic between the facilities cultivation and field cultivation, however, there were significant differences on the quantity characteristics,especially for the characteristics about the leaf length and leaf width, between the facilities cultivation and field cultivation. So, in winter, considering the types and characteristics was necessary, and in order to utilize the data obtained, DUS test based on testing types should be performed.
2011, 2(4): 338-341.
doi: 10.15886/j.cnki.rdswxb.2011.04.016
Abstract:
In our study, the flower buds were used as explants and the tissue culture of mokara was performed. The results indicated that it was on culture medium （MS+6-BA 3.0 mg·L-1+NAA 0.3 mg·L-1） that the flower buds of Mokara differentiated into the original bulbs or multiple shoot clumps after 40 days; it was on culture medium （MS+6-BA 3.0 mg·L-1+NAA 0.3 mg·L-1+Coconut water 100 mL·L-1） that the proliferation effect of protocorm was best; it was on culture medium （1/3MS+ HY-NO.1 1 g·L-1+ Coconut water 100 mL·L-1） that the seedling effect of budlet was best; it was on culture medium （HY-NO.1 3g·L-1+Coconut water 100 mL·L-1+banana 40 g·L-1+potato 20 g·L-1） that the root effect of plantlet was best.
In our study, the flower buds were used as explants and the tissue culture of mokara was performed. The results indicated that it was on culture medium （MS+6-BA 3.0 mg·L-1+NAA 0.3 mg·L-1） that the flower buds of Mokara differentiated into the original bulbs or multiple shoot clumps after 40 days; it was on culture medium （MS+6-BA 3.0 mg·L-1+NAA 0.3 mg·L-1+Coconut water 100 mL·L-1） that the proliferation effect of protocorm was best; it was on culture medium （1/3MS+ HY-NO.1 1 g·L-1+ Coconut water 100 mL·L-1） that the seedling effect of budlet was best; it was on culture medium （HY-NO.1 3g·L-1+Coconut water 100 mL·L-1+banana 40 g·L-1+potato 20 g·L-1） that the root effect of plantlet was best.
2011, 2(4): 342-348.
doi: 10.15886/j.cnki.rdswxb.2011.04.017
Abstract:
To investigate the mechanism of the natural regeneration of indigenous mangroves in Sonneratia apetala Buch.-Ham plantations and the effects of S.apetala. The method of survey plots was performed to analysis 14 years of S.apetala plantations in Dongzhai Harbor in November 2010. The results indicated that the community of S.apetala plantations is in the early stages of natural regeneration; there were correlation between provenance distance, elevation, forest spacing, the number of pneumatophores and natural regeneration of indigenous mangroves which were as followed: Shrubs:Y1=0.998X1+0.324X2+8.54X3-0.122X1X3-75.953;Seedlings:Y2=151.934-0.565X1-1.682X4; the natural regeneration was accelerated by the S.apetala forest planted with circulation and strips.
To investigate the mechanism of the natural regeneration of indigenous mangroves in Sonneratia apetala Buch.-Ham plantations and the effects of S.apetala. The method of survey plots was performed to analysis 14 years of S.apetala plantations in Dongzhai Harbor in November 2010. The results indicated that the community of S.apetala plantations is in the early stages of natural regeneration; there were correlation between provenance distance, elevation, forest spacing, the number of pneumatophores and natural regeneration of indigenous mangroves which were as followed: Shrubs:Y1=0.998X1+0.324X2+8.54X3-0.122X1X3-75.953;Seedlings:Y2=151.934-0.565X1-1.682X4; the natural regeneration was accelerated by the S.apetala forest planted with circulation and strips.
2011, 2(4): 349-354.
doi: 10.15886/j.cnki.rdswxb.2011.04.019
Abstract:
The objective of the study was to clone full-length cDNA of Cdc42（Cell division cycle 42） gene of buffalo and analyze this gene.RT-PCR were performed to clone Cdc42 cDNA from buffalo, and the cloned Cdc42 cDNA sequence had been sent to GeneBank and got a accession number:JN380079.The bioinformatics analysis results showed that the Cdc42 cDNA sequence was 626 bp length,containing an open reading frame of 576 bp,encoding 191 amino acids.Relative molecular weight was 21.3 kD,pI was 6.16.Further analysis results indicated that the homology of Cdc42 gene of buffalo are 99.65%, 96.70%, 94.97% and 94.10%, compared to cattle, dog, mouse and human, respectively; the protein was non-transmembrane protein, which include a RHO domain. These results set a strong basis for further research of the function of Cdc42 of buffalo.
The objective of the study was to clone full-length cDNA of Cdc42（Cell division cycle 42） gene of buffalo and analyze this gene.RT-PCR were performed to clone Cdc42 cDNA from buffalo, and the cloned Cdc42 cDNA sequence had been sent to GeneBank and got a accession number:JN380079.The bioinformatics analysis results showed that the Cdc42 cDNA sequence was 626 bp length,containing an open reading frame of 576 bp,encoding 191 amino acids.Relative molecular weight was 21.3 kD,pI was 6.16.Further analysis results indicated that the homology of Cdc42 gene of buffalo are 99.65%, 96.70%, 94.97% and 94.10%, compared to cattle, dog, mouse and human, respectively; the protein was non-transmembrane protein, which include a RHO domain. These results set a strong basis for further research of the function of Cdc42 of buffalo.
2011, 2(4): 355-359.
doi: 10.15886/j.cnki.rdswxb.2011.04.006
Abstract:
The crude anthocyanins from Rambutan fruit pericarp tissues were natural food additives. In our report, the extraction conditions by enzyme solution were optimized. The results showed that the maximum absorption wave length of the anthocyanins was 535 nm,the optimal enzyme was cellulase, and the optimum conditions of enzymatic extraction were as follows: the ratio of dry pericarp to extracting solution was 1∶ 15（g∶ mL）, pH value of enzyme solution was 5.2, the amount of enzyme was 5%, the digest temperature was 55 ℃, the digest time was 150 min. It was under the condition that the content of anthocyanins reached 1.14 mg·g-1 fruit pericarp.
The crude anthocyanins from Rambutan fruit pericarp tissues were natural food additives. In our report, the extraction conditions by enzyme solution were optimized. The results showed that the maximum absorption wave length of the anthocyanins was 535 nm,the optimal enzyme was cellulase, and the optimum conditions of enzymatic extraction were as follows: the ratio of dry pericarp to extracting solution was 1∶ 15（g∶ mL）, pH value of enzyme solution was 5.2, the amount of enzyme was 5%, the digest temperature was 55 ℃, the digest time was 150 min. It was under the condition that the content of anthocyanins reached 1.14 mg·g-1 fruit pericarp.
2011, 2(4): 360-363.
doi: 10.15886/j.cnki.rdswxb.2011.04.007
Abstract:
In order to study the effect of physical treatments on the rheology characteristics of konjac glucomannan, after konjac purified powder was prepared onto hydrosol,which was treated with the High Hydrostatic Pressure（HHP）, and rheometer was used to determine its related rheological properties. The results showed that the effect of the high hydrostatic pressure treatment on the rheology properties of konjac glucomannan was very significant, in which the effect of pressure was more significant that that of time, and when the pressure was 100 MPa, the change of rheology properties attained the highest point.
In order to study the effect of physical treatments on the rheology characteristics of konjac glucomannan, after konjac purified powder was prepared onto hydrosol,which was treated with the High Hydrostatic Pressure（HHP）, and rheometer was used to determine its related rheological properties. The results showed that the effect of the high hydrostatic pressure treatment on the rheology properties of konjac glucomannan was very significant, in which the effect of pressure was more significant that that of time, and when the pressure was 100 MPa, the change of rheology properties attained the highest point.
2011, 2(4): 364-372.
doi: 10.15886/j.cnki.rdswxb.2011.04.004
Abstract:
Around the world, abiotic stress is increasing as a result of a global climate changes, which has become the one of most important challenges for the world. The studies on plant response to abiotic stress can help us to understand how plant tolerant the abiotic stress. In the paper, the progresses of the study on plant abiotic stress response genes were reviewed.
Around the world, abiotic stress is increasing as a result of a global climate changes, which has become the one of most important challenges for the world. The studies on plant response to abiotic stress can help us to understand how plant tolerant the abiotic stress. In the paper, the progresses of the study on plant abiotic stress response genes were reviewed.
2011, 2(4): 373-377.
doi: 10.15886/j.cnki.rdswxb.2011.04.013
Abstract:
The introgression of transgenes into common wild rice （Oryza rufipogon） and its wild relatives or weeds through pollen mediated gene flow is a major concern in environmental risk assessment of transgenic crops. In the report, the progresses on the gene flow from transgenic rice to common wild rice and its close relatives were reviewed and the strategies for controlling transgene flow were also discussed.
The introgression of transgenes into common wild rice （Oryza rufipogon） and its wild relatives or weeds through pollen mediated gene flow is a major concern in environmental risk assessment of transgenic crops. In the report, the progresses on the gene flow from transgenic rice to common wild rice and its close relatives were reviewed and the strategies for controlling transgene flow were also discussed.
2011, 2(4): 378-382.
doi: 10.15886/j.cnki.rdswxb.2011.04.018
Abstract:
In this paper, the research progresses in the study of wave attenuation effect of mangroves were reviewed. Most of such researches were implemented at South-east Asia. The attenuation effect was studied mainly by analyzing field monitoring data, the correlation between attenuation rate and vegetation, and hydrological traits. And some other researches were carried out by developing theoretical model to estimate the attenuation effect.
In this paper, the research progresses in the study of wave attenuation effect of mangroves were reviewed. Most of such researches were implemented at South-east Asia. The attenuation effect was studied mainly by analyzing field monitoring data, the correlation between attenuation rate and vegetation, and hydrological traits. And some other researches were carried out by developing theoretical model to estimate the attenuation effect.
2011, 2(4): 383-388.
doi: 10.15886/j.cnki.rdswxb.2011.04.005
Abstract:
In the report, the reinforcement of natural rubber latex by inorganic and organic particles, resin emulsion and other fillers were reviewed, and the future research direction of latex reinforcement technique was also analyzed simply.
In the report, the reinforcement of natural rubber latex by inorganic and organic particles, resin emulsion and other fillers were reviewed, and the future research direction of latex reinforcement technique was also analyzed simply.
2011, 2(4): 389-394.
Abstract:
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