Tissue Culture of Mokara

Key words: 
• Mokara /
• tissue culture /
• protocorm

Abstract: In our study, the flower buds were used as explants and the tissue culture of mokara was performed. The results indicated that it was on culture medium （MS+6-BA 3.0 mg·L^-1+NAA 0.3 mg·L^-1） that the flower buds of Mokara differentiated into the original bulbs or multiple shoot clumps after 40 days; it was on culture medium （MS+6-BA 3.0 mg·L^-1+NAA 0.3 mg·L^-1+Coconut water 100 mL·L^-1） that the proliferation effect of protocorm was best; it was on culture medium （1/3MS+ HY-NO.1 1 g·L^-1+ Coconut water 100 mL·L^-1） that the seedling effect of budlet was best; it was on culture medium （HY-NO.1 3g·L^-1+Coconut water 100 mL·L^-1+banana 40 g·L^-1+potato 20 g·L^-1） that the root effect of plantlet was best.