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Volume 8 Issue 3
Sep.  2017
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Article Navigation >  Journal of Tropical Biology  > 2017  >  8(3): 359-363,377

ZHANG Xiaobei, ZHU Guopeng, ZHU Zhixin, NAN Wenzhuo, WU Zhipeng. Determination of the Polysaccharide Content of Sweet Potato Leaves by Using 3'5'-Dinitrosalicylic Acid(DNS)[J]. Journal of Tropical Biology, 2017, 8(3): 359-363,377. doi: 10.15886/j.cnki.rdswxb.2017.03.018
Citation: ZHANG Xiaobei, ZHU Guopeng, ZHU Zhixin, NAN Wenzhuo, WU Zhipeng. Determination of the Polysaccharide Content of Sweet Potato Leaves by Using 3'5'-Dinitrosalicylic Acid(DNS)[J]. Journal of Tropical Biology, 2017, 8(3): 359-363,377. doi: 10.15886/j.cnki.rdswxb.2017.03.018
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Determination of the Polysaccharide Content of Sweet Potato Leaves by Using 3'5'-Dinitrosalicylic Acid(DNS)

doi: 10.15886/j.cnki.rdswxb.2017.03.018

  • Institute of Tropical Agriculture and Forestry/Ministry of Education Key Laboratory of Protection, Development and Utilization of Tropical Crop Germplasm Resources, Hainan University, Haikou, Hainan 570228, China

  • Received Date: 2017-03-29
  • Rev Recd Date: 2017-06-21
  • The reducing sugar and total sugar contents of the leaves of vegetable sweet potato（Dioscorea esculenta（Lour.） Burkill） were determined by using the 3’5’-dinitrosalicylic acid（DNS） method to indirectly determine the leaf content of polysaccharides of three vegetable sweet potato varieties.The optimum conditions for the determination of reducing sugar and total contents were as follows:Optimum wavelength 493 nm,DNS reagent 2.0 mL,coloring reaction10 min,and setting time 10 min.For the reducing sugar and total sugar,the best extraction conditions would be boiling water bath for 30 min.The total sugar was hydrolyzed with hydrochloric acid at a rate of 2.0 mL,and extracted in boiling water bath for 20 min.With this method the polysaccharide contents of the leaves of the three vegetable sweet potato varieties were 12.63,13.49 and 18.51 mg·g-1 FW respectively.The repeatability and recovery of this method meet the test requirements.It is concluded that this method can be used for the determination of polysaccharide content in vegetable sweet potato leaves,and can also be used for the determination of reducing sugar and total sugar.
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Determination of the Polysaccharide Content of Sweet Potato Leaves by Using 3'5'-Dinitrosalicylic Acid(DNS)

DOI: 10.15886/j.cnki.rdswxb.2017.03.018
  • Institute of Tropical Agriculture and Forestry/Ministry of Education Key Laboratory of Protection, Development and Utilization of Tropical Crop Germplasm Resources, Hainan University, Haikou, Hainan 570228, China
  • Received Date: 2017-03-29
  • Rev Recd Date: 2017-06-21

Abstract: The reducing sugar and total sugar contents of the leaves of vegetable sweet potato（Dioscorea esculenta（Lour.） Burkill） were determined by using the 3’5’-dinitrosalicylic acid（DNS） method to indirectly determine the leaf content of polysaccharides of three vegetable sweet potato varieties.The optimum conditions for the determination of reducing sugar and total contents were as follows:Optimum wavelength 493 nm,DNS reagent 2.0 mL,coloring reaction10 min,and setting time 10 min.For the reducing sugar and total sugar,the best extraction conditions would be boiling water bath for 30 min.The total sugar was hydrolyzed with hydrochloric acid at a rate of 2.0 mL,and extracted in boiling water bath for 20 min.With this method the polysaccharide contents of the leaves of the three vegetable sweet potato varieties were 12.63,13.49 and 18.51 mg·g-1 FW respectively.The repeatability and recovery of this method meet the test requirements.It is concluded that this method can be used for the determination of polysaccharide content in vegetable sweet potato leaves,and can also be used for the determination of reducing sugar and total sugar.

ZHANG Xiaobei, ZHU Guopeng, ZHU Zhixin, NAN Wenzhuo, WU Zhipeng. Determination of the Polysaccharide Content of Sweet Potato Leaves by Using 3'5'-Dinitrosalicylic Acid(DNS)[J]. Journal of Tropical Biology, 2017, 8(3): 359-363,377. doi: 10.15886/j.cnki.rdswxb.2017.03.018
Citation: ZHANG Xiaobei, ZHU Guopeng, ZHU Zhixin, NAN Wenzhuo, WU Zhipeng. Determination of the Polysaccharide Content of Sweet Potato Leaves by Using 3'5'-Dinitrosalicylic Acid(DNS)[J]. Journal of Tropical Biology, 2017, 8(3): 359-363,377. doi: 10.15886/j.cnki.rdswxb.2017.03.018
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