南海4种共生虫黄藻株系的分离培养和生理特性

布海陆; 王珺; 周智

doi:10.15886/j.cnki.rdswxb.20230117

南海4种共生虫黄藻株系的分离培养和生理特性

doi: 10.15886/j.cnki.rdswxb.20230117

海南大学海洋科学与工程学院, 海口 570228

基金项目:

42161144006)

国家自然科学基金(42076145

详细信息

第一作者:
布海陆(1999-),男,海南大学海洋科学与工程学院2021级硕士研究生。E-mail:919306863@qq.com

通信作者:
王珺(1972-),女,硕士,高级实验师。研究方向:水产养殖学。E-mail:72206wj@163.com

周智(1983-),男,博士,研究员。研究方向:海洋生物学。E-mail:zhouzhi@hainanu.edu.cn

中图分类号: Q178.53

Isolated culture and physiological characteristics of four symbiotic Symbiodiniaceae strains from South China Sea

College of Marine Science and Engineering, Hainan University, Haikou, Hainan 570228, China

摘要: 为了探究不同虫黄藻的种间差异及其生理特点，从南海的多种刺胞生物中分离获取4株单克隆虫黄藻株系，并围绕其系统发育学、形态学及多种生理学特征展开了一系列研究。结果表明，4株虫黄藻分别归为Symbiodinium属（A3型，编号：HNUA3-1）、Breviolum属（B1型，编号：HNUB1-1）、Cladocopium属（C1型，编号：HNUC1-1）、Durusdinium属（D1型，编号：HNUD1-1）。光学显微镜及透射电子显微镜观察均显示4种藻株在形态学特征上具有很高的相似性，但比生长速率存在显著性差异，反映出虫黄藻种间的特异性生长特征。其中，藻株Breviolum sp. HNUB1-1比生长速率最高，其细胞密度在第3、5、7天均显著高于其他3种藻株（P <0.05），而藻株Symbiodinium sp. HNUA3-1的比生长速率次之，藻株Cladocopium sp. HNUC1-1与Durusdinium sp. HNUD1-1的比生长速率最低，7 d内的两者细胞密度无显著差异。此外，不同藻株之间的最大光量子产量（Fv/Fm）也存在物种特异性，同等条件下，藻株Durusdinium sp. HNUD1-1的Fv/Fm远低于其他3种藻株，反映了其潜在耐热性的光合生理特征。本研究为丰富我国南海虫黄藻种质资源，明确虫黄藻种间差异及特征提供理论依据，可为我国南海珊瑚礁生态系统的恢复提供基础数据支持。
- 虫黄藻 /
- Symbiodinium /
- Breviolum /
- Cladocopium /
- Durusdinium
Abstract: In recent years, coral bleaching events induced by marine heat waves have occurred frequently, and Symbiodiniaceae, as algal symbionts of coral hosts, has been proved to play an important role in controlling coral environmental tolerance. In order to explore the differences among different species of Symbiodiniaceae and their physiological characteristics, four monoclonal strains of Symbiodiniaceae were isolated and obtained from different cnidaria organisms in the South China Sea, and a series of investigations were conducted on their phylogenetic, morphological and various physiological characteristics. The results exhibited that the four strains of Symbiodiniaceae were Symbiodinium（type A3, HNUA3-1）, Breviolum（type B1, HNUB1-1）, Cladocopium（type C1, HNUC1-1） and Durusdinium（type D1, HNUD1-1）, respectively. Observations by light microscopy and transmission electron microscopy revealed that the four strains had higher similarity in morphological characteristics but there were significant differences in specific growth rates, reflecting the species-specific growth characteristics. The specific growth rate of Breviolum sp. HNUB1-1 was the highest, and the cell density of Breviolum sp. HNUB1-1 was significantly higher than that of the other three strains on the 3rd, 5th and 7th day（P ＜ 0.05）, the specific growth rate of Symbiodinium sp. HNUA3-1 was second highest, the specific growth rate of Cladocopium sp. HNUC1-1 and Durusdinium sp. HNUD1-1 was the lowest, and there was no significant difference in cell density within 7 days of culture. Furthermore, different strains waere also specie-specific in maximum quantum efficiency of Photosystem II（Fv/Fm）. Under the same conditions, the Fv/Fm of the Durusdinium sp. HNUD1-1 was significantly lower than that of the other three strains, reflecting the photosynthetic and physiological characteristics of its potential heat resistance. This study provided theoretical basis for enriching the resources of Symbiodiniaceae in the South China Sea, and clarifying the differences and characteristics of the Symbiodiniaceae, which can be used to provide basic data support for the conservation and restoration of the coral reef ecosystem in the South China Sea.
- Symbiodiniaceae /
- Symbiodinium /
- Breviolum /
- Cladocopium /
- Durusdinium

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南海4种共生虫黄藻株系的分离培养和生理特性

doi: 10.15886/j.cnki.rdswxb.20230117

第一作者:
布海陆(1999-),男,海南大学海洋科学与工程学院2021级硕士研究生。E-mail:919306863@qq.com

通信作者:
王珺(1972-),女,硕士,高级实验师。研究方向:水产养殖学。E-mail:72206wj@163.com

周智(1983-),男,博士,研究员。研究方向:海洋生物学。E-mail:zhouzhi@hainanu.edu.cn

Isolated culture and physiological characteristics of four symbiotic Symbiodiniaceae strains from South China Sea

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南海4种共生虫黄藻株系的分离培养和生理特性

doi: 10.15886/j.cnki.rdswxb.20230117

海南大学海洋科学与工程学院, 海口 570228

作者简介:
布海陆(1999-),男,海南大学海洋科学与工程学院2021级硕士研究生。E-mail:919306863@qq.com

通讯作者: 王珺(1972-),女,硕士,高级实验师。研究方向:水产养殖学。E-mail:72206wj@163.com; 周智(1983-),男,博士,研究员。研究方向:海洋生物学。E-mail:zhouzhi@hainanu.edu.cn

English Abstract

Isolated culture and physiological characteristics of four symbiotic Symbiodiniaceae strains from South China Sea

College of Marine Science and Engineering, Hainan University, Haikou, Hainan 570228, China

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南海4种共生虫黄藻株系的分离培养和生理特性

doi: 10.15886/j.cnki.rdswxb.20230117

第一作者: 布海陆(1999-),男,海南大学海洋科学与工程学院2021级硕士研究生。E-mail:919306863@qq.com

通信作者: 王珺(1972-),女,硕士,高级实验师。研究方向:水产养殖学。E-mail:72206wj@163.com 周智(1983-),男,博士,研究员。研究方向:海洋生物学。E-mail:zhouzhi@hainanu.edu.cn

Isolated culture and physiological characteristics of four symbiotic Symbiodiniaceae strains from South China Sea

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出版历程

南海4种共生虫黄藻株系的分离培养和生理特性

doi: 10.15886/j.cnki.rdswxb.20230117

海南大学 海洋科学与工程学院, 海口 570228

作者简介: 布海陆(1999-),男,海南大学海洋科学与工程学院2021级硕士研究生。E-mail:919306863@qq.com

通讯作者: 王珺(1972-),女,硕士,高级实验师。研究方向:水产养殖学。E-mail:72206wj@163.com; 周智(1983-),男,博士,研究员。研究方向:海洋生物学。E-mail:zhouzhi@hainanu.edu.cn

English Abstract

Isolated culture and physiological characteristics of four symbiotic Symbiodiniaceae strains from South China Sea

College of Marine Science and Engineering, Hainan University, Haikou, Hainan 570228, China

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目录

第一作者:
布海陆(1999-),男,海南大学海洋科学与工程学院2021级硕士研究生。E-mail:919306863@qq.com

通信作者:
王珺(1972-),女,硕士,高级实验师。研究方向:水产养殖学。E-mail:72206wj@163.com

周智(1983-),男,博士,研究员。研究方向:海洋生物学。E-mail:zhouzhi@hainanu.edu.cn

海南大学海洋科学与工程学院, 海口 570228

作者简介:
布海陆(1999-),男,海南大学海洋科学与工程学院2021级硕士研究生。E-mail:919306863@qq.com