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金花茶(Camellia nitidissima Chi)属于山茶科、山茶属、金花茶组、金花茶系落叶小灌木植物,主要分布在中国南部和越南北部。金花茶是一种天然草药,2009年被卫生部批准为新食品来源[1],列为国家一级保护植物,被誉为“植物界的大熊猫”[2]。金花茶对于调节人体血脂、血糖、胆固醇,增强机体免疫力具有明显的效果,且能够抗菌消炎、清热解毒、利尿消肿、增强肝肾脏活力、防止血动脉硬化,抑制肿瘤生长等[3]。金花茶的叶和花中主要化学成分有黄酮、皂苷、多糖和茶多酚等[4]。虽然国内外学者对山茶属的其他种群的化学成分及其药理活性进行了比较深入的研究[5-8],但对于金花茶的单体化合物研究却鲜有报道。为了进一步探明金花茶的有效物质基础,笔者对金花茶花的化学成分进行分析,旨在为金花茶花的开发利用提供依据。
Chemical Composition of Lignans from the Flowers of Camellia nitidissima
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摘要: 为了研究金花茶花中的木脂素类化学成分,采用硅胶柱、Sephadex LH-20凝胶柱、C18反相色谱柱、半制备高压液相色谱等色谱方法对金花茶花的化学成分进行分离纯化,同时运用1H-NMR,13C-NMR,ESI-MS等多种波谱技术鉴定化合物的结构。共得到8个木脂素类化合物,分别鉴定为:1)桉脂素,2)(+)-diasyringaresinol,3)(+)-isoeucommin A,4)Pinoresinol 4-O-glucoside,5)7S, 8R, 8 ′R-(-)-lariciresinol-4′-O-D-glucopyranoside,6)(+)-Isolariciresinol 9-O-β-D-glucopyranoside,7)(+)-Isolariciresinol 9′-O-β-D-glucopyranoside,8)3′, 4-O-dimethylcedrusin。本实验8个木脂素类化合物均为首次从该植物中分离得到。Abstract: Flowers of Camellia nitidissima Chi were extracted, and their chemical constituents were then isolated and purified by silica gel, Sephadex LH-20 gel, C18 reversed silica gel, and semi-preparative HPLC. The structures of their chemical constituents were determined by using iH-NMR, 13C-NMR and ESI-MS spectra. Eight lignans were identified from the extracts of the flowers, and their structures were elucidated from the spectra as: eudesmin (1), (+)-diasyringaresinol (2), (+)-isoeucommin A (3), Pinoresinol 4-O-glucoside (4), 7S, 8R, 8′R-(-)-lariciresinol-4′-O-D-glucopyranoside (5), (+)-Isolariciresinol 9-O-β-D-glucopyranoside (6), (+)-Isolariciresinol 9′-O-β-D-glucopyranoside (7), and 3′, 4-O-dimethylcedrusin (8). All these chemical compounds were isolated from C. nitidissima Chi for the first time.
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Key words:
- Camellia nitidissima Chi /
- lignans /
- isolation /
- structural identification
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