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木薯MKK家族基因的鉴定及表达分析

侯鹏宇,  于心怡,  肖晓蓉,  郑琳琳,  陈银华

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文章导航 > 热带生物学报  > 2019 >  10(2): 119-126
侯鹏宇, 于心怡, 肖晓蓉, 郑琳琳, 陈银华. 木薯MKK家族基因的鉴定及表达分析[J]. 热带生物学报, 2019, 10(2): 119-126. doi: 10.15886/j.cnki.rdswxb.2019.02.004
引用本文: 侯鹏宇, 于心怡, 肖晓蓉, 郑琳琳, 陈银华. 木薯MKK家族基因的鉴定及表达分析[J]. 热带生物学报, 2019, 10(2): 119-126. doi: 10.15886/j.cnki.rdswxb.2019.02.004
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HOU Pengyu, YU Xinyi, XIAO Xiaorong, ZHENG Linlin, CHEN Yinhua. Identification and Expression Analysis of MKK Genes in Cassava[J]. Journal of Tropical Biology, 2019, 10(2): 119-126. doi: 10.15886/j.cnki.rdswxb.2019.02.004
Citation: HOU Pengyu, YU Xinyi, XIAO Xiaorong, ZHENG Linlin, CHEN Yinhua. Identification and Expression Analysis of MKK Genes in Cassava[J]. Journal of Tropical Biology, 2019, 10(2): 119-126. doi: 10.15886/j.cnki.rdswxb.2019.02.004
shu

木薯MKK家族基因的鉴定及表达分析

doi: 10.15886/j.cnki.rdswxb.2019.02.004

  • 海南大学 热带农林学院/海南省热带生物资源利用重点实验室, 海口 570228

基金项目: 

国家自然科学基金(31560497)

海南省研究生创新科研课题(Hys2018-02)

详细信息
    第一作者:

    侯鹏宇(1992-),女,海南大学热带农林学院2016级硕士研究生,E-mail:18789078283@163.com

    通信作者:

    郑琳琳(1983-),女,博士,讲师.研究方向:分子遗传学,E-mail:hildagarde@163.com

  • 中图分类号: S553

Identification and Expression Analysis of MKK Genes in Cassava

  • Institute of Tropical Agriculture and Forestry, Hainan University/Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Haikou, Hainan 570228, China

  • 摘要: MAPK级联传导通路是广泛存在于真核生物体内的信号传导途径。根据木薯全基因组数据,借鉴拟南芥中MAPKK (MKK)家族的基因序列,通过生物信息学方法对木薯中MKK家族成员进行全面的鉴定以及系统进化树分析。通过激素处理及病原菌接种来分析MKK家族各基因的表达模式。结果表明,木薯总共编码11个MKK基因,这些基因集中分布于木薯第3,4,6,10,12,16,17条染色体上。MKK家族各基因在木薯受到激素处理及病原菌处理后的表达分析结果表明,MKKs能够响应ABA,JA及病原菌信号,对ACC信号则不敏感。MKK4,MKK5,MKK8,MKK9,MKK11可能参与木薯相关防御病原菌及激素信号传导通路。
    Abstract: The mitogen-activated protein kinase（MAPK） cascade pathway is a signaling pathway widely present in eukaryotic organisms. Based on cassava genomic data, a comprehensive identification and phylogenetic tree analysis of mitogen-activated protein kinase kinase kinase（MKK） family members in cassava was carried out by bioinformatics methods. At the same time, the expression pattern of each gene of the MKK family was analyzed through hormone treatment and pathogen inoculation. The results showed that cassava encoded 11 MKK genes, which were distributed on chromosomes 3, 4, 6, 10, 12, 16, and 17 of cassava. The expression analysis showed that MKKs were responsive to ABA, JA and pathogen signal but not sensitive to ACC signal, of which MKK4, MKK5, MKK8, MKK9 and MKK11 may be involved in cassava-related pathogens defense pathways and hormone signaling pathways.
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    木薯MKK家族基因的鉴定及表达分析

    doi: 10.15886/j.cnki.rdswxb.2019.02.004
    • 海南大学 热带农林学院/海南省热带生物资源利用重点实验室, 海口 570228
      • 基金项目:

      国家自然科学基金(31560497)

      海南省研究生创新科研课题(Hys2018-02)

      作者简介:

      侯鹏宇(1992-),女,海南大学热带农林学院2016级硕士研究生,E-mail:18789078283@163.com

      通讯作者: 郑琳琳(1983-),女,博士,讲师.研究方向:分子遗传学,E-mail:hildagarde@163.com
    • 收稿日期: 2019-01-28
    • 修回日期: 2019-02-27

    • 中图分类号: S553

    摘要: MAPK级联传导通路是广泛存在于真核生物体内的信号传导途径。根据木薯全基因组数据,借鉴拟南芥中MAPKK (MKK)家族的基因序列,通过生物信息学方法对木薯中MKK家族成员进行全面的鉴定以及系统进化树分析。通过激素处理及病原菌接种来分析MKK家族各基因的表达模式。结果表明,木薯总共编码11个MKK基因,这些基因集中分布于木薯第3,4,6,10,12,16,17条染色体上。MKK家族各基因在木薯受到激素处理及病原菌处理后的表达分析结果表明,MKKs能够响应ABA,JA及病原菌信号,对ACC信号则不敏感。MKK4,MKK5,MKK8,MKK9,MKK11可能参与木薯相关防御病原菌及激素信号传导通路。

    English Abstract

    侯鹏宇, 于心怡, 肖晓蓉, 郑琳琳, 陈银华. 木薯MKK家族基因的鉴定及表达分析[J]. 热带生物学报, 2019, 10(2): 119-126. doi: 10.15886/j.cnki.rdswxb.2019.02.004
    引用本文: 侯鹏宇, 于心怡, 肖晓蓉, 郑琳琳, 陈银华. 木薯MKK家族基因的鉴定及表达分析[J]. 热带生物学报, 2019, 10(2): 119-126. doi: 10.15886/j.cnki.rdswxb.2019.02.004
    shu
    HOU Pengyu, YU Xinyi, XIAO Xiaorong, ZHENG Linlin, CHEN Yinhua. Identification and Expression Analysis of MKK Genes in Cassava[J]. Journal of Tropical Biology, 2019, 10(2): 119-126. doi: 10.15886/j.cnki.rdswxb.2019.02.004
    Citation: HOU Pengyu, YU Xinyi, XIAO Xiaorong, ZHENG Linlin, CHEN Yinhua. Identification and Expression Analysis of MKK Genes in Cassava[J]. Journal of Tropical Biology, 2019, 10(2): 119-126. doi: 10.15886/j.cnki.rdswxb.2019.02.004
    shu

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