香蕉枯萎病菌候选效应蛋白FoSSP80能抑制植物免疫反应

赵阳; 刘爽; 王志彪; 伍俊宇; 陈代朋; 郑丽

doi:10.15886/j.cnki.rdswxb.20240019

香蕉枯萎病菌候选效应蛋白FoSSP80能抑制植物免疫反应

doi: 10.15886/j.cnki.rdswxb.20240019

赵阳^1,2,
刘爽^1,2,
王志彪^1,2,
伍俊宇^1,2,
陈代朋^2,,
郑丽^1,

1. 海南大学南繁学院/三亚南繁研究院, 海南三亚 572025 中国;
2. 海南大学热带农林学院, 海南儋州 571737 中国

基金项目:

海南大学协同创新中心项目(XTCX2022NYB10)

海南省重点研发项目(ZDYF2022XDNY242)

国家自然科学基金地区项目(32360642)

详细信息

第一作者:
赵阳(1997-),男,海南大学热带农林学院2021级硕士研究生。E-mail:zy155956@163.com

通信作者:
陈代朋(1987-),男,讲师。研究方向:植物病理学。E-mail:chen.daipeng@163.com

郑丽(1985-),女,教授。研究方向:植物病理学。E-mail:zhenglihappy0617@126.com

中图分类号: S432.1

Functional characterization of candidate effector FoSSP80 in Fusarium oxysporum f. sp. cubense

1. Nanfan College/Sanya Nanfan Institute, Hainan University, Sanya, Hainan 572025, China;
2. School of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737, China

摘要: 香蕉枯萎病是香蕉的主要病害之一。香蕉枯萎病菌（Fusarium oxysporum f. sp. cubense, Foc）产生的效应蛋白在其侵染香蕉的过程中发挥了重要作用。实验室前期在Foc中筛选到候选效应蛋白FoSSP80，生物信息学分析发现FoSSP80含有信号肽、无跨膜结构域；利用酵母分泌系统证实了FoSSP80具有分泌功能；在烟草中瞬时表达能够抑制由BAX引起的细胞程序性死亡（PCD），且FoSSP80可以抑制活性氧（ROS）的积累和胼胝质的沉积；与野生型和回补菌株相比敲除突变体ΔFoSSP80在菌落形态、生长速率和分生孢子产生和致病性方面都没有显著差异。研究结果表明FoSSP80是一个经典分泌蛋白，且可以抑制植物的免疫反应。
- 香蕉 /
- 枯萎病 /
- 效应蛋白 /
- 植物免疫
Abstract: Fusarium wilt of banana is one of the major diseases infecting banana. Effectors produced by Fusarium oxysporum f. sp. Cubense（Foc） play an important role during its infection of banana. Previously, a candidate effector FoSSP80 in Foc was screened. Bioinformatics analysis revealed that the FoSSP80 contained a signal peptide without transmembrane structural domains. Furthermore, the FoSSP80 was confirmed to have a secretory function by using the yeast secretion system. Transient expression of FoSSP80 in Nicotiana benthamiana led to inhibition of programmed cell death（PCD） induced by BAX. Moreover, FoSSP80 inhibited the accumulation of reactive oxygen species（ROS） and the callose deposition. The knockout mutant ΔFoSSP80 did not show significant differences in colony morphology, growth rate, and conidial production and pathogenicity compared with the wild-type and complement strains. These results suggested that FoSSP80 is a typical secretory protein and can inhibit plant defense responses.
- banana /
- Fusarium wilt /
- effector /
- plant immunity

[1]	李华平,李云锋,聂燕芳.香蕉枯萎病的发生及防控研究现状[J]. 华南农业大学学报, 2019, 40(5):128-136.
[2]	GHAG S B, SHEKHAWAT U K S, GANAPATHI T R.Fusarium wilt of banana:biology, epidemiology and management[J]. International Journal of Pest Management,2015, 61(3):250-263.
[3]	PLOETZ R C. Management of Fusarium wilt of banana:a review with special reference to tropical race 4[J]. Crop Protection, 2015, 73:7-15.
[4]	ATKINSON N J, URWIN P E. The interaction of plant biotic and abiotic stresses:from genes to the field[J]. Journal of Experimental Botany, 2012, 63(10):3523-3543.
[5]	JONES J D G, DANGL J L. The plant immune system[J]. Nature, 2006, 444(7117):323-329.
[6]	BOLLER T, HE S Y. Innate immunity in plants:an arms race between pattern recognition receptors in plants and effectors in microbial pathogens[J]. Science, 2009, 324(5928):742-744.
[7]	HOUTERMAN P M, SPEIJER D, DEKKER H L, et al.The mixed xylem sap proteome of Fusarium oxysporuminfected tomato plants[J]. Molecular Plant Pathology,2007, 8(2):215-221.
[8]	SCHMIDT S M, HOUTERMAN P M, SCHREIVER I, et al. MITEs in the promoters of effector genes allow prediction of novel virulence genes in Fusarium oxysporum[J]. BMC Genomics, 2013, 14:119.
[9]	REP M, VAN DER DOES H C, MEIJER M, et al. A small,cysteine-rich protein secreted by Fusarium oxysporum during colonization of xylem vessels is required for I-3-mediated resistance in tomato[J]. Molecular Microbiology,2004, 53(5):1373-1383.
[10]	HOUTERMAN P M, MA L, VAN OOIJEN G, et al. The effector protein Avr2 of the xylem-colonizing fungus Fusarium oxysporum activates the tomato resistance protein I-2 intracellularly[J]. The Plant Journal:for Cell and Molecular Biology, 2009, 58(6):970-978.
[11]	MA L, HOUTERMAN P M, GAWEHNS F, et al. The AVR2-SIX5 gene pair is required to activate I-2-mediated immunity in tomato[J]. The New Phytologist, 2015,208(2):507-518.
[12]	GAWEHNS F, HOUTERMAN P M, ICHOU F A, et al.The Fusarium oxysporum effector Six6 contributes to virulence and suppresses I-2-mediated cell death[J]. Molecular Plant-Microbe Interactions:MPMI, 2014,27(4):336-348.
[13]	HOU X, AN B, WANG Q, et al. SGE1 is involved in conidiation and pathogenicity of Fusarium oxysporum f.sp. cubense[J]. Canadian Journal of Microbiology, 2018,64(5):349-357.
[14]	AN B, HOU X, GUO Y, et al. The effector SIX8 is required for virulence of Fusarium oxysporum f. sp.cubense tropical race 4 to Cavendish banana[J]. Fungal Biology, 2019, 123(5):423-430.
[15]	GUO L, WANG J, LIANG C, et al. Fosp9, a novel secreted protein, is essential for the full virulence of Fusarium oxysporum f. sp. cubense on banana(Musa spp.) [J]. Applied and Environmental Microbiology,2022, 88(6):e0060421.
[16]	WANG Y, ZHANG X, WANG T, et al. The small secreted protein FoSsp1 elicits plant defenses and negatively regulates pathogenesis in Fusarium oxysporum f.sp. cubense(Foc4) [J]. Frontiers in Plant Science, 2022,13:873451.
[17]	WANG T, XU Y, ZHAO Y, et al. Systemic screening of Fusarium oxysporum candidate effectors reveals FoSSP17 that suppresses plant immunity and contributes to virulence[J]. Phytopathology Research, 2023, 5(1):42.
[18]	TAMURA K, STECHER G, KUMAR S. MEGA11:molecular evolutionary genetics analysis version 11[J]. Molecular Biology and Evolution, 2021, 38(7):3022-3027.
[19]	王田,符俐盈,赵阳,等.香蕉枯萎病菌候选效应蛋白FoSSP20的鉴定和功能初探[J]. 热带生物学报, 2024,15(1):85-93.
[20]	GU B, KALE S D, WANG Q, et al. Rust secreted protein Ps87 is conserved in diverse fungal pathogens and contains a RXLR-like motif sufficient for translocation into plant cells[J]. PLoS One, 2011, 6(11):e27217.
[21]	WEI Y, LIU W, HU W, et al. The chaperone MeHSP90recruits MeWRKY20 and MeCatalase1 to regulate drought stress resistance in cassava[J]. The New Phytologist, 2020, 226(2):476-491.
[22]	DONG J, CHEN W. The role of autophagy in chloroplast degradation and chlorophagy in immune defenses during Pst DC3000(AvrRps4) infection[J]. PLoS One, 2013,8(8):e73091.
[23]	GOSWAMI R S. Targeted gene replacement in fungi using a split-marker approach[J]. Methods in Molecular Biology, 2012, 835:255-269.
[24]	YUN Y, LIU Z, ZHANG J, et al. The MAPKK FgMkk1 of Fusarium graminearum regulates vegetative differentiation, multiple stress response, and virulence via the cell wall integrity and high-osmolarity glycerol signaling pathways[J]. Environmental Microbiology, 2014, 16(7):2023-2037.
[25]	CHEN D, WANG Y, ZHOU X, et al. The Sch9 kinase regulates conidium size, stress responses, and pathogenesis in Fusarium graminearum[J]. PLoS One, 2014, 9(8):e105811.
[26]	ZHANG L, CENCI A, ROUARD M, et al. Transcriptomic analysis of resistant and susceptible banana corms in response to infection by Fusarium oxysporum f. sp.cubense tropical race 4[J]. Scientific Reports, 2019,9(1):8199.
[27]	LEE M C S, MILLER E A, GOLDBERG J, et al. Bidirectional protein transport between the ER and Golgi[J]. Annual Review of Cell and Developmental Biology, 2004,20:87-123.
[28]	LACOMME C, SANTA CRUZ S. Bax-induced cell death in tobacco is similar to the hypersensitive response[J]. Proceedings of the National Academy of Sciences of the United States of America, 1999, 96(14):7956-7961.
[29]	ABRAMOVITCH R B, KIM Y J, CHEN S, et al. Pseudomonas type III effector AvrPtoB induces plant disease susceptibility by inhibition of host programmed cell death[J]. The EMBO Journal, 2003, 22(1):60-69.
[30]	LI X, JIN C, YUAN H, et al. The barley powdery mildew effectors CSEP0139 and CSEP0182 suppress cell death and promote B. graminis fungal virulence in plants[J]. Phytopathology Research, 2021, 3(1):7.
[31]	LING T, VANDELLE E, BELLIN D, et al. Nitric oxide produced during the hypersensitive response modulates the plant signaling network and inhibits the pathogen's virulence machinery[J]. Nitric Oxide, 2012, 27:S9.

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香蕉枯萎病菌候选效应蛋白FoSSP80能抑制植物免疫反应

doi: 10.15886/j.cnki.rdswxb.20240019

第一作者:
赵阳(1997-),男,海南大学热带农林学院2021级硕士研究生。E-mail:zy155956@163.com

通信作者:
陈代朋(1987-),男,讲师。研究方向:植物病理学。E-mail:chen.daipeng@163.com

郑丽(1985-),女,教授。研究方向:植物病理学。E-mail:zhenglihappy0617@126.com

Functional characterization of candidate effector FoSSP80 in Fusarium oxysporum f. sp. cubense

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香蕉枯萎病菌候选效应蛋白FoSSP80能抑制植物免疫反应

doi: 10.15886/j.cnki.rdswxb.20240019

1. 海南大学南繁学院/三亚南繁研究院, 海南三亚 572025 中国;

2. 海南大学热带农林学院, 海南儋州 571737 中国

作者简介:
赵阳(1997-),男,海南大学热带农林学院2021级硕士研究生。E-mail:zy155956@163.com

通讯作者: 陈代朋(1987-),男,讲师。研究方向:植物病理学。E-mail:chen.daipeng@163.com; 郑丽(1985-),女,教授。研究方向:植物病理学。E-mail:zhenglihappy0617@126.com

English Abstract

Functional characterization of candidate effector FoSSP80 in Fusarium oxysporum f. sp. cubense

1. Nanfan College/Sanya Nanfan Institute, Hainan University, Sanya, Hainan 572025, China;

2. School of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737, China

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香蕉枯萎病菌候选效应蛋白FoSSP80能抑制植物免疫反应

doi: 10.15886/j.cnki.rdswxb.20240019

第一作者: 赵阳(1997-),男,海南大学热带农林学院2021级硕士研究生。E-mail:zy155956@163.com

通信作者: 陈代朋(1987-),男,讲师。研究方向:植物病理学。E-mail:chen.daipeng@163.com 郑丽(1985-),女,教授。研究方向:植物病理学。E-mail:zhenglihappy0617@126.com

Functional characterization of candidate effector FoSSP80 in Fusarium oxysporum f. sp. cubense

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香蕉枯萎病菌候选效应蛋白FoSSP80能抑制植物免疫反应

doi: 10.15886/j.cnki.rdswxb.20240019

1. 海南大学 南繁学院/三亚南繁研究院, 海南 三亚 572025 中国; 2. 海南大学 热带农林学院, 海南 儋州 571737 中国

作者简介: 赵阳(1997-),男,海南大学热带农林学院2021级硕士研究生。E-mail:zy155956@163.com

通讯作者: 陈代朋(1987-),男,讲师。研究方向:植物病理学。E-mail:chen.daipeng@163.com; 郑丽(1985-),女,教授。研究方向:植物病理学。E-mail:zhenglihappy0617@126.com

English Abstract

Functional characterization of candidate effector FoSSP80 in Fusarium oxysporum f. sp. cubense

1. Nanfan College/Sanya Nanfan Institute, Hainan University, Sanya, Hainan 572025, China; 2. School of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737, China

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目录

第一作者:
赵阳(1997-),男,海南大学热带农林学院2021级硕士研究生。E-mail:zy155956@163.com

通信作者:
陈代朋(1987-),男,讲师。研究方向:植物病理学。E-mail:chen.daipeng@163.com

郑丽(1985-),女,教授。研究方向:植物病理学。E-mail:zhenglihappy0617@126.com

1. 海南大学南繁学院/三亚南繁研究院, 海南三亚 572025 中国;

2. 海南大学热带农林学院, 海南儋州 571737 中国

作者简介:
赵阳(1997-),男,海南大学热带农林学院2021级硕士研究生。E-mail:zy155956@163.com

1. Nanfan College/Sanya Nanfan Institute, Hainan University, Sanya, Hainan 572025, China;

2. School of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737, China