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香蕉枯萎病菌候选效应蛋白FoSSP80能抑制植物免疫反应

赵阳 刘爽 王志彪 伍俊宇 陈代朋 郑丽

赵阳, 刘爽, 王志彪, 伍俊宇, 陈代朋, 郑丽. 香蕉枯萎病菌候选效应蛋白FoSSP80能抑制植物免疫反应[J]. 热带生物学报, 2025, 16(1): 87-97. doi: 10.15886/j.cnki.rdswxb.20240019
引用本文: 赵阳, 刘爽, 王志彪, 伍俊宇, 陈代朋, 郑丽. 香蕉枯萎病菌候选效应蛋白FoSSP80能抑制植物免疫反应[J]. 热带生物学报, 2025, 16(1): 87-97. doi: 10.15886/j.cnki.rdswxb.20240019
ZHAO Yang, LIU Shuang, WANG Zhibiao, WU Junyu, CHEN Daipeng, ZHENG Li. Functional characterization of candidate effector FoSSP80 in Fusarium oxysporum f. sp. cubense[J]. Journal of Tropical Biology, 2025, 16(1): 87-97. doi: 10.15886/j.cnki.rdswxb.20240019
Citation: ZHAO Yang, LIU Shuang, WANG Zhibiao, WU Junyu, CHEN Daipeng, ZHENG Li. Functional characterization of candidate effector FoSSP80 in Fusarium oxysporum f. sp. cubense[J]. Journal of Tropical Biology, 2025, 16(1): 87-97. doi: 10.15886/j.cnki.rdswxb.20240019

香蕉枯萎病菌候选效应蛋白FoSSP80能抑制植物免疫反应

doi: 10.15886/j.cnki.rdswxb.20240019
基金项目: 

海南大学协同创新中心项目(XTCX2022NYB10)

海南省重点研发项目(ZDYF2022XDNY242)

国家自然科学基金地区项目(32360642)

详细信息
    第一作者:

    赵阳(1997-),男,海南大学热带农林学院2021级硕士研究生。E-mail:zy155956@163.com

    通信作者:

    陈代朋(1987-),男,讲师。研究方向:植物病理学。E-mail:chen.daipeng@163.com

    郑丽(1985-),女,教授。研究方向:植物病理学。E-mail:zhenglihappy0617@126.com

  • 中图分类号: S432.1

Functional characterization of candidate effector FoSSP80 in Fusarium oxysporum f. sp. cubense

  • 摘要: 香蕉枯萎病是香蕉的主要病害之一。香蕉枯萎病菌(Fusarium oxysporum f. sp. cubense, Foc)产生的效应蛋白在其侵染香蕉的过程中发挥了重要作用。实验室前期在Foc中筛选到候选效应蛋白FoSSP80,生物信息学分析发现FoSSP80含有信号肽、无跨膜结构域;利用酵母分泌系统证实了FoSSP80具有分泌功能;在烟草中瞬时表达能够抑制由BAX引起的细胞程序性死亡(PCD),且FoSSP80可以抑制活性氧(ROS)的积累和胼胝质的沉积;与野生型和回补菌株相比敲除突变体ΔFoSSP80在菌落形态、生长速率和分生孢子产生和致病性方面都没有显著差异。研究结果表明FoSSP80是一个经典分泌蛋白,且可以抑制植物的免疫反应。
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    香蕉枯萎病菌候选效应蛋白FoSSP80能抑制植物免疫反应

    doi: 10.15886/j.cnki.rdswxb.20240019
      基金项目:

      海南大学协同创新中心项目(XTCX2022NYB10)

      海南省重点研发项目(ZDYF2022XDNY242)

      国家自然科学基金地区项目(32360642)

      作者简介:

      赵阳(1997-),男,海南大学热带农林学院2021级硕士研究生。E-mail:zy155956@163.com

      通讯作者: 陈代朋(1987-),男,讲师。研究方向:植物病理学。E-mail:chen.daipeng@163.com; 郑丽(1985-),女,教授。研究方向:植物病理学。E-mail:zhenglihappy0617@126.com
    • 中图分类号: S432.1

    摘要: 香蕉枯萎病是香蕉的主要病害之一。香蕉枯萎病菌(Fusarium oxysporum f. sp. cubense, Foc)产生的效应蛋白在其侵染香蕉的过程中发挥了重要作用。实验室前期在Foc中筛选到候选效应蛋白FoSSP80,生物信息学分析发现FoSSP80含有信号肽、无跨膜结构域;利用酵母分泌系统证实了FoSSP80具有分泌功能;在烟草中瞬时表达能够抑制由BAX引起的细胞程序性死亡(PCD),且FoSSP80可以抑制活性氧(ROS)的积累和胼胝质的沉积;与野生型和回补菌株相比敲除突变体ΔFoSSP80在菌落形态、生长速率和分生孢子产生和致病性方面都没有显著差异。研究结果表明FoSSP80是一个经典分泌蛋白,且可以抑制植物的免疫反应。

    English Abstract

    赵阳, 刘爽, 王志彪, 伍俊宇, 陈代朋, 郑丽. 香蕉枯萎病菌候选效应蛋白FoSSP80能抑制植物免疫反应[J]. 热带生物学报, 2025, 16(1): 87-97. doi: 10.15886/j.cnki.rdswxb.20240019
    引用本文: 赵阳, 刘爽, 王志彪, 伍俊宇, 陈代朋, 郑丽. 香蕉枯萎病菌候选效应蛋白FoSSP80能抑制植物免疫反应[J]. 热带生物学报, 2025, 16(1): 87-97. doi: 10.15886/j.cnki.rdswxb.20240019
    ZHAO Yang, LIU Shuang, WANG Zhibiao, WU Junyu, CHEN Daipeng, ZHENG Li. Functional characterization of candidate effector FoSSP80 in Fusarium oxysporum f. sp. cubense[J]. Journal of Tropical Biology, 2025, 16(1): 87-97. doi: 10.15886/j.cnki.rdswxb.20240019
    Citation: ZHAO Yang, LIU Shuang, WANG Zhibiao, WU Junyu, CHEN Daipeng, ZHENG Li. Functional characterization of candidate effector FoSSP80 in Fusarium oxysporum f. sp. cubense[J]. Journal of Tropical Biology, 2025, 16(1): 87-97. doi: 10.15886/j.cnki.rdswxb.20240019
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