Isolation,culture and identification of mitral valve interstitial cells from Wuzhishan pig

CHEN Shumin; CHEN Yu; ZHAO Yuanjie; LIU Mengmeng

doi:10.15886/j.cnki.rdswxb.20240049

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Myxomatous mitral valve disease（MMVD） is one of the most common heart diseases in mammals,which can lead to heart failure and sudden death in clinical practice. The extracellular matrix remodeling caused by mitral stromal cells（MVICs） activation is the key pathological change in the development of MMVD.Therefore, this study intended to establish a separation and culture system for primary MVICs of Wuzhishan pig to obtain stable cultured MVICs, providing cell materials for in vitro research on MMVD. The results showed that the mitral valve cells of Wuzhishan pig isolated by the present method were CD31 negative and Vimentin positive, which were consistent with the characteristics of MVIC. Compared with the medium with different serum concentrations, the complete medium containing 10% FBS serum was more favorable to the proliferation activity of MVICs cells, and could support the stable culture of MVICs in vitro for at least 20 generations. The primary MVICs of Wuzhishan pig obtained in this study can provide an in vitro research platform for the study of the pathological mechanism of mitral valve disease and the further development of targeted drugs.

Isolation,culture and identification of mitral valve interstitial cells from Wuzhishan pig

School of Tropical Agriculture and Forestry/SARA/SRR, Hainan University, Haikou, Hainan 570228, China

Received Date: 2024-03-22

Rev Recd Date: 2024-04-08

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Abstract: Myxomatous mitral valve disease（MMVD） is one of the most common heart diseases in mammals,which can lead to heart failure and sudden death in clinical practice. The extracellular matrix remodeling caused by mitral stromal cells（MVICs） activation is the key pathological change in the development of MMVD.Therefore, this study intended to establish a separation and culture system for primary MVICs of Wuzhishan pig to obtain stable cultured MVICs, providing cell materials for in vitro research on MMVD. The results showed that the mitral valve cells of Wuzhishan pig isolated by the present method were CD31 negative and Vimentin positive, which were consistent with the characteristics of MVIC. Compared with the medium with different serum concentrations, the complete medium containing 10% FBS serum was more favorable to the proliferation activity of MVICs cells, and could support the stable culture of MVICs in vitro for at least 20 generations. The primary MVICs of Wuzhishan pig obtained in this study can provide an in vitro research platform for the study of the pathological mechanism of mitral valve disease and the further development of targeted drugs.

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Reference (23)

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Isolation,culture and identification of mitral valve interstitial cells from Wuzhishan pig

doi: 10.15886/j.cnki.rdswxb.20240049

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