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Volume 10 Issue 1
Mar.  2019
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LIN Yu, LI Zengping, WU Ruhui, ZHANG Yu. Identification and Biological Characteristics of the Pathogen Causing Phomopsis leaf spot in Khaya senegalensis[J]. Journal of Tropical Biology, 2019, 10(1): 34-40. doi: 10.15886/j.cnki.rdswxb.2019.01.006
Citation: LIN Yu, LI Zengping, WU Ruhui, ZHANG Yu. Identification and Biological Characteristics of the Pathogen Causing Phomopsis leaf spot in Khaya senegalensis[J]. Journal of Tropical Biology, 2019, 10(1): 34-40. doi: 10.15886/j.cnki.rdswxb.2019.01.006

Identification and Biological Characteristics of the Pathogen Causing Phomopsis leaf spot in Khaya senegalensis

doi: 10.15886/j.cnki.rdswxb.2019.01.006
  • Received Date: 2018-11-02
  • Rev Recd Date: 2018-12-04
  • The pathogen of Phomopsis leaf spot in Khaya senegalensis was identified and its biological characteristics were determined. The pathogen was identified as Diaporthe musigena based on pathogenicity measurement and morphological observation as well as molecular identification. The biological observation showed that the optimum conditions for the pathogen growth were temperature 28 ℃, pH5, Soybean peptone as nitrogen source, sucrose as carbon source. Alternation of light and darkness inhibited the mycelial growth of the pathogen.
  • [1] 戚佩坤, 姜子德, 向梅梅, 等.中国真菌志 (第三十四卷拟茎点霉属) [M].北京:科学出版社, 2007:1-18.92-93.
    [2] 弓明钦.非洲楝病害及其防治[J].热带林业科技, 1983 (2):23-28.
    [3] 方中达.植病研究方法「M」北京:中国农业出版社, 1998.
    [4] 管斌, 徐超, 张红岩, 等.红叶石楠叶斑病病原菌分离鉴定及致病性测定研究[J].西部林业科学, 2013, 42 (2):56-61.
    [5] 陆家云.植物病原真菌学[M].北京:中国农业出版社, 2001.
    [6] 劳佳萍.植物内生真菌拟茎点霉生物多样性研究[D].浙江:浙江大学.2007.
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    [8] CROUS P W.Fungal Planet description sheets:69–91[J].Persoonia 2011, 26:108-156.
    [9] 何彩梅, 魏大巧, 李海燕, 等.云南元江干热河谷五种优势植物的内生真菌多样性[J].生态学报, 2011, 31 (12):3315-3321.
    [10] HUANG FENG, HOU XIN, MEGAN M DEWDNEY, et al.Diaporthe species occurring on citrus in China[J].Fungal Diversity, 2013, 61 (1), 237-250.
    [11] LI S X, GLEN L, HARTMAN G L, et al.Aggressiveness of Phomopsis longicolla and other Phomopsis spp.on Sophean[J].Plant Disease, 2010, 94 (8):1035-1040.
    [12] 李雪光, 潘凤娟, 宋洁, 等.拟茎点霉属及其病害研究进展[J].大豆科技, 2012 (6):32-37.
    [13] 谢玲, 黄思良, 岑贞陆, 等.芒果褐色蒂腐病菌 (Phomop-sis mangiferae) 生物学特性研究[J].微生物学杂志, 2002, 22 (1):15-17.
    [14] UECKER F A.A world list of Phomopsis names with noteson nomenclature, morphology and hiology[J].Mycol Mem, 1988, 13:1-231
    [15] 张居念, 陈艺晖, 林艺芬, 等.龙眼拟茎点霉 (Phomopsis longanae Chi) 的生物学特性研究[J].热带作物学报, 2013, 34 (4):695-699.
    [16] 牛晓庆, 唐庆华, 余凤玉, 等.油棕叶斑病的病原鉴定及其生物学特性[J].江西农业学报, 2011, 23 (11):103-105

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Identification and Biological Characteristics of the Pathogen Causing Phomopsis leaf spot in Khaya senegalensis

doi: 10.15886/j.cnki.rdswxb.2019.01.006

Abstract: The pathogen of Phomopsis leaf spot in Khaya senegalensis was identified and its biological characteristics were determined. The pathogen was identified as Diaporthe musigena based on pathogenicity measurement and morphological observation as well as molecular identification. The biological observation showed that the optimum conditions for the pathogen growth were temperature 28 ℃, pH5, Soybean peptone as nitrogen source, sucrose as carbon source. Alternation of light and darkness inhibited the mycelial growth of the pathogen.

LIN Yu, LI Zengping, WU Ruhui, ZHANG Yu. Identification and Biological Characteristics of the Pathogen Causing Phomopsis leaf spot in Khaya senegalensis[J]. Journal of Tropical Biology, 2019, 10(1): 34-40. doi: 10.15886/j.cnki.rdswxb.2019.01.006
Citation: LIN Yu, LI Zengping, WU Ruhui, ZHANG Yu. Identification and Biological Characteristics of the Pathogen Causing Phomopsis leaf spot in Khaya senegalensis[J]. Journal of Tropical Biology, 2019, 10(1): 34-40. doi: 10.15886/j.cnki.rdswxb.2019.01.006
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