Tissue Culture and Rapid Propagation of Ananas comosus ’Apple’

Key words: 
• Ananas comosus ’Apple’ /
• tissue culture /
• rapid propagation

Abstract: The apical buds and suckers of pineapple cultivar ’Apple’（ Ananas comosus ’Apple’） were inoculated onto MS medium containing 6-BA 3 mg·L^-1and 30 g·L^-1sucrose for inducing shoots. The shoots were bourgeoned after cultured for 30 d. They were then transferred onto MS medium supplemented with 6-BA 2 mg·L^-1,NAA 0. 05 mg·L^-1and 30 g·L^-1sucrose for inducing cluster buds,and the cluster buds were produced after 30- 40 d of culture. The proliferation ratio of the cluster buds was about 3- 5 per month. The cluster buds were cultured on MS medium containing 6-BA 1 mg ·L^-1,AD 3 mg·L^-1and 30 g · L^-1sucrose for about25- 30 d to produce strong shoots. These shoots were transferred onto 1 /2 MS rooting medium containing NAA0. 50 mg·L^-1,30 g·L^-1banana juice and 30 g·L^-1sucrose,and the roots were induced to strike after about30 d of culture. The rooted plantlets had about 95% of survival rate when transferred onto the soil.