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Volume 5 Issue 2
Jun.  2014
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Article Navigation >  Journal of Tropical Biology  > 2014  >  5(2): 174-178

HE Wang, CHEN Xinping, FU Shengmiao. Chromosome Analysis of Chorionic Villi in 50 Cases of Missed Abortions and Improvement of an Experiment Method[J]. Journal of Tropical Biology, 2014, 5(2): 174-178. doi: 10.15886/j.cnki.rdswxb.2014.02.017
Citation: HE Wang, CHEN Xinping, FU Shengmiao. Chromosome Analysis of Chorionic Villi in 50 Cases of Missed Abortions and Improvement of an Experiment Method[J]. Journal of Tropical Biology, 2014, 5(2): 174-178. doi: 10.15886/j.cnki.rdswxb.2014.02.017
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Chromosome Analysis of Chorionic Villi in 50 Cases of Missed Abortions and Improvement of an Experiment Method

doi: 10.15886/j.cnki.rdswxb.2014.02.017

  • Central Laboratory, Hainan Provincial People's Hospital, Haikou 570311, China

  • Received Date: 2014-02-09
  • Chorionic villi tissues from 50 cases of missed abortions were sampled and cultured to improve methods for tissue culture and chromosome preparation and to analyze the relationship between the chromosome change and the missed abortion. All the samples are divided into both control and experiment groups each. They were cultured for 5 10 days after trypsin digestion. The cells from the chorionic villi tissues in the control group were harvested by using the conventional method,while the cells in the experiment group were then subcultured and harvested by using a modified method. The cells from these two groups were analysed by G-banding after their chromosomes were prepared,and their mitotic indexes were calculated and compared. The results showed that the harvesting method in the experiment group is simple and easy to operate and produces higher mitotic indexes and better chromosome morphology and dispersion than the conventional method in the control group. Analysis of the chorionic villi from these 50 cases of missed abortions showed that 14 cases are of autosomal anomalies,8 cases of sex chromosome abnormalities,3 cases of chromosomal structural abnormality,1 case of triploid,and 24 cases of normal karyotypes.
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Chromosome Analysis of Chorionic Villi in 50 Cases of Missed Abortions and Improvement of an Experiment Method

doi: 10.15886/j.cnki.rdswxb.2014.02.017
  • Central Laboratory, Hainan Provincial People's Hospital, Haikou 570311, China
  • Received Date: 2014-02-09

Abstract: Chorionic villi tissues from 50 cases of missed abortions were sampled and cultured to improve methods for tissue culture and chromosome preparation and to analyze the relationship between the chromosome change and the missed abortion. All the samples are divided into both control and experiment groups each. They were cultured for 5 10 days after trypsin digestion. The cells from the chorionic villi tissues in the control group were harvested by using the conventional method,while the cells in the experiment group were then subcultured and harvested by using a modified method. The cells from these two groups were analysed by G-banding after their chromosomes were prepared,and their mitotic indexes were calculated and compared. The results showed that the harvesting method in the experiment group is simple and easy to operate and produces higher mitotic indexes and better chromosome morphology and dispersion than the conventional method in the control group. Analysis of the chorionic villi from these 50 cases of missed abortions showed that 14 cases are of autosomal anomalies,8 cases of sex chromosome abnormalities,3 cases of chromosomal structural abnormality,1 case of triploid,and 24 cases of normal karyotypes.

HE Wang, CHEN Xinping, FU Shengmiao. Chromosome Analysis of Chorionic Villi in 50 Cases of Missed Abortions and Improvement of an Experiment Method[J]. Journal of Tropical Biology, 2014, 5(2): 174-178. doi: 10.15886/j.cnki.rdswxb.2014.02.017
Citation: HE Wang, CHEN Xinping, FU Shengmiao. Chromosome Analysis of Chorionic Villi in 50 Cases of Missed Abortions and Improvement of an Experiment Method[J]. Journal of Tropical Biology, 2014, 5(2): 174-178. doi: 10.15886/j.cnki.rdswxb.2014.02.017
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