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Volume 5 Issue 2
Jun.  2014
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Article Navigation >  Journal of Tropical Biology  > 2014  >  5(2): 162-165,161

XU Jingwen, LIAN Wenjun, CAO Yuxin, XIA Zhihui, HUANG Xi. Epoxy-resin Embedded Tissue Section and Staining of the Barks of Hevea brasiliensis[J]. Journal of Tropical Biology, 2014, 5(2): 162-165,161. doi: 10.15886/j.cnki.rdswxb.2014.02.014
Citation: XU Jingwen, LIAN Wenjun, CAO Yuxin, XIA Zhihui, HUANG Xi. Epoxy-resin Embedded Tissue Section and Staining of the Barks of Hevea brasiliensis[J]. Journal of Tropical Biology, 2014, 5(2): 162-165,161. doi: 10.15886/j.cnki.rdswxb.2014.02.014
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Epoxy-resin Embedded Tissue Section and Staining of the Barks of Hevea brasiliensis

doi: 10.15886/j.cnki.rdswxb.2014.02.014

  • Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources/College of Agronomy, Hainan University, Haikou 570228, China

  • Received Date: 2014-02-15
  • Tissue sectioning and staining are basic technologies for dissecting plant tissue and organic structure.Rubber trees（Hevea brasiliensis Müll. Arg.） are the most important commercial source of natural rubber,and they produce and store latex in laticifers in the bark. Paraffin section stained with I- Br is usually used for observation of laticifer under light microscopy. The procedures of this method are complicated and time-consuming.Therefore,a technology for tissue section with tissue embedded in epoxy resin without using I- Br staining is introduced for laticifer observation under light microscopy,including fixation,dehydration,infiltration,embedding,polymerization,sectioning,staining,and mounting. Laticifer,xylem,cambium,vessel,phloemray radial,primary phloem ray fiber,cortex,and epidermis were well distinguished on the epoxy resin-embedded tissue sections by using 3 different stains. Furthermore,numerous cells and their inclusions that were scarcely observed in paraffin section,such as tannin,chloroplast,etc were also observed on the tissues embedded in epoxy resin. This technology is an improvement in paraffin-embedded tissue section under light microscopy for the bark of the rubber tree.
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Epoxy-resin Embedded Tissue Section and Staining of the Barks of Hevea brasiliensis

doi: 10.15886/j.cnki.rdswxb.2014.02.014
  • Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources/College of Agronomy, Hainan University, Haikou 570228, China
  • Received Date: 2014-02-15

Abstract: Tissue sectioning and staining are basic technologies for dissecting plant tissue and organic structure.Rubber trees（Hevea brasiliensis Müll. Arg.） are the most important commercial source of natural rubber,and they produce and store latex in laticifers in the bark. Paraffin section stained with I- Br is usually used for observation of laticifer under light microscopy. The procedures of this method are complicated and time-consuming.Therefore,a technology for tissue section with tissue embedded in epoxy resin without using I- Br staining is introduced for laticifer observation under light microscopy,including fixation,dehydration,infiltration,embedding,polymerization,sectioning,staining,and mounting. Laticifer,xylem,cambium,vessel,phloemray radial,primary phloem ray fiber,cortex,and epidermis were well distinguished on the epoxy resin-embedded tissue sections by using 3 different stains. Furthermore,numerous cells and their inclusions that were scarcely observed in paraffin section,such as tannin,chloroplast,etc were also observed on the tissues embedded in epoxy resin. This technology is an improvement in paraffin-embedded tissue section under light microscopy for the bark of the rubber tree.

XU Jingwen, LIAN Wenjun, CAO Yuxin, XIA Zhihui, HUANG Xi. Epoxy-resin Embedded Tissue Section and Staining of the Barks of Hevea brasiliensis[J]. Journal of Tropical Biology, 2014, 5(2): 162-165,161. doi: 10.15886/j.cnki.rdswxb.2014.02.014
Citation: XU Jingwen, LIAN Wenjun, CAO Yuxin, XIA Zhihui, HUANG Xi. Epoxy-resin Embedded Tissue Section and Staining of the Barks of Hevea brasiliensis[J]. Journal of Tropical Biology, 2014, 5(2): 162-165,161. doi: 10.15886/j.cnki.rdswxb.2014.02.014
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