Screening of Anti-browning Agents in Banana Callus-inducing Culture

Key words: 
• banana /
• callus induction /
• browning /
• anti-browning agents

Abstract: Anti-browning agents,AgNO3（10,20 mg·L^-1）,activated carbon（1,2,3 g·L^-1）,Polyvinyl Pyrrolidone（PVP）（1,2 g·L^-1）,Vitamin C（100,200 mg·L^-1） were supplemented in the banana culture medium to inhibit explant browning of banana during the callus inducing process. Ten days after cultured the explants treated with anti-browning agents showed a lower browning rate than those in the control. This indicated that all the anti-browning agents were effective in inhibiting explant browning at the early stage of callus inducing culture. However,with the time of tissue culture going on,the anti-browning agents tended to be significantly different in inhibition of explant browning,and 40 days after callus-inducing culture the activated carbon treatments showed the highest inhibition of explant browning,of which the 1g·L^-1activated carbon was optimum with a browning rate of only 20% of the banana explants.