Discrimination of Cassia Seed Polysaccharides by Fingerprint Infrared Spectroscopy
doi: 10.15886/j.cnki.rdswxb.2014.02.003
- Received Date: 2014-04-30
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Key words:
- polysaccharide /
- infrared analysis /
- principle component analysis /
- similarity
Abstract: The polysaccharides from cassia seeds(seeds of Cassia obtusifolia or C. tora) were analyzed by using fingerprint infrared spectrometry. A model for infrared fingerprint spectra of polysaccharides from 10 batches of cassia seeds collected in Jiangxi was established and standardized with a similarity ranging from 0. 935 to 0. 998.With this model the cassia seeds polysaccharide from unknown source was evaluated,and the similarity was merely 0. 782. Furthermore,11 co-existing peaks were chosen from the IR fingerprints of the polysaccharides from 10 batches of cassia seeds collected in Jiangxi and resorted to principal component analysis and hierarchical cluster analysis. The results showed that the cassia seeds from the unknown source was different from those collected in Jiangxi and was in a different category. The fingerprinting method and principle component analysis could be applied to supervise the quality of the products or raw materials that have bioactive components of polysaccharides.
Citation: | LI Pingfan, GU Zongzhu. Discrimination of Cassia Seed Polysaccharides by Fingerprint Infrared Spectroscopy[J]. Journal of Tropical Biology, 2014, 5(2): 166-169. doi: 10.15886/j.cnki.rdswxb.2014.02.003 |