Structure and Function of LEA Proteins in Higher Plants

ZHOU Li; JI Xiang-nan; HE Fei; PAN Qiu-hong; DUAN Zhang-qing

doi:10.15886/j.cnki.rdswxb.2012.02.005

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Volume 3 Issue 2

Jun. 2012

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Article Navigation > Journal of Tropical Biology > 2012 > 3(2): 191-196

ZHOU Li, JI Xiang-nan, HE Fei, PAN Qiu-hong, DUAN Zhang-qing. Structure and Function of LEA Proteins in Higher Plants[J]. Journal of Tropical Biology, 2012, 3(2): 191-196. doi: 10.15886/j.cnki.rdswxb.2012.02.005

Citation:

ZHOU Li, JI Xiang-nan, HE Fei, PAN Qiu-hong, DUAN Zhang-qing. Structure and Function of LEA Proteins in Higher Plants[J]. Journal of Tropical Biology, 2012, 3(2): 191-196. doi: 10.15886/j.cnki.rdswxb.2012.02.005

Structure and Function of LEA Proteins in Higher Plants

doi: 10.15886/j.cnki.rdswxb.2012.02.005

Centre for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China

Received Date: 2012-02-20

Abstract

Late embryogenesis abundant proteins（LEA proteins） are a group of stress-responsive proteins in higher plants that are induced by environmental stress.These proteins can enhance plant tolerance against environmental stress via stabilizing cell membrane and binding ions.The category and structure of LEA proteins are summarized herein.The researches conducted in recent years are reviewed about the roles of LEA proteins in plant response to drought,low temperature and other environmental stresses.
- plant resistance,
- late embryogenesis abundant protein,

References

[1]	DURE III L,GRENWAY S C,GALAU G A.Developmental biochemistry of cottonseed embryogenesis and germination:chan-ging messenger ribonucleic acid populations as shown by in vitro and in vivo protein synthesis[J].Biochemistry,1981,20:4162-4168.
[2]	GALAU G A,HUGHES D W,DURE III L.Abscisic acid induction of cloned late embryogenesis-abundant(LEA)mRNAs[J].Plant Molecular Biology,1986,7:155-170.
[3]	WISE M J.LEAping to conclusions:a computational reanalysis of late embryogenesis abundant proteins and their possible roles[J].BMC Bioinformatics,2003,4:52.
[4]	ZEGZOUTI H,JONES B,MARTY C,et al.ER5,a tomato cDNA encoding an ethylene-responsive LEA-like protein:charac-terization and expression in response to drought,ABA and wounding[J].Plant Molecular Biology,1997,35:847-854.
[5]	PARK J A,CHO S K,KIM J E,et al.Isolation of cDNAs differentially expressed in response to drought stress and character-ization of the Ca-LEAL1gene encoding a new family of atypical LEA-like protein homologue in hot pepper(Capsicum annuum L.cv.Pukang)[J].Plant Science,2003,165:471-481.
[6]	RAYNAL M,GAUBIER P,GRELLET F,et al.Nucleotide sequence of a radish cDNA clone coding for a late embryogenesis abundant(LEA)protein[J].Nucleic Acids Research,1900,18:20.
[7]	TUNNACLIFFE A,WISE M J.The continuing conundrum of the LEA proteins[J].Naturwissenschaften,2007,94:791-812.
[8]	于丽霞,汤晓倩,武晓璐,等.茭白lEA3基因全长cDNA的分离克隆及序列分析[J].中国蔬菜,2010(6):14-18.
[9]	刘金仙,阙友雄,郭晋隆,等.甘蔗胚胎晚期丰富蛋白(LEA)cDNA全长克隆及表达特性[J].农业生物技术学报,2009,17(5):836-842.
[10]	MAITRA N,CUSHMAN J C.Isolation and characterization of a drought-induced soybean cDNA encoding a D95famile late-embryogenesis-abundant protein[J].Plant physiology,1994,106:805-806.
[11]	TOLLETER D,HINCHA D K,MACHEREL D.A mitochondrial late embryogenesis abundant protein stabilizes model mem-branes in the dry state[J].Biochimica et Biophysica Acta,2010,1798:1926-1933.
[12]	GRELET J,BENAMAR A,TEYSSIER E,et al.Identification in pea seed mitochondria of a late-embryogenesis abundant protein able to protect enzymes from drying[J].Plant Physiology,2005,137:157-167.
[13]	GOYAL K,WALTON L J,Tunnacliffe A.LEA proteins prevent protein aggregation due to water stress[J].Journal of Bio-chemistry,2005,388:151-157.
[14]	BAKER J,STEELE C,DURE III L.Sequence and characterization of6LEA proteins and their genes from cotton[J].Plant Molecular Biology,1988,11:277-291.
[15]	DURE III L,CROUCH M,HARADA J,et al.Common amino acid sequence domains among the LEA proteins of higher plants[J].Plant Molecular Biology,1989,12:475-486.
[16]	BRAY E A.Molecular responses to water deficit[J].Plant Physiology,1993,103(4):1035-1040.
[17]	HUNDERMARK M,HINCHA D K.LEA(late embryogenesis abundant)proteins and their encoding genes in Arabidopsis thaliana[J].BMC Genomics,2008,9:118.
[18]	RORAT T.Plant dehydrins-tissue location,structure and function[J].Cellular&Molecular Biology Letters,2006,11:536-556.
[19]	VASEVA I I,GRIGOROVA B S,SIMOVA-STOILOVA L P,et al.Abscisic acid and late embryogenesis abundant protein profile changes in winter wheat under progressive drought stress[J].Plant Biology,2010,12:698-707.
[20]	KOAG M C,FENTON R D,WILKENS S,et al.The binding of maize DHN1to lipid vesicles.gain of structure and lipid specificity[J].Plant Physiology,2003,131:309-316.
[21]	ALI-BENALI M A,ALARY R,JOUDRIER P,et al.comparative expression of five Lea genes during wheat seed develop-ment and in response to abiotic stress by real-time quantitative RT-PCR[J].Biochimicaet Biophysica Acta,2005,1730:56-65.
[22]	BRINI F,SAIBI W,AMARA I,et al.Wheat dehydrin DHN-5exerts a heat-protective effect onβ-glucosidase and glucose oxidase activities[J].Bioscience Biotechnology and Biochemistry,2010,74:1050-1054.
[23]	SHAO H B,LIANG Z S,SHAO M A.LEA proteins in higher plants:Structure,function,gene expression and regulation[J].Colloids and Surfaces B:Biointerfaces,2005,45:131-135.
[24]	OLVERA-CARRILLO Y,CAMPOS F,REYES J L,et al.Functional analysis of the group4late embryogenesis abundant proteins reveals their relevance in the adaptive response during water deficit in Arabidopsis[J].Plant Physiology,2010,154:373-390.
[25]	GALAU G A,WANG H Y C,HUGHES D W.Cotton LEA5and LEA14encode atypical late embryogenesis-abundant proteins[J].Plant Physiology,1993,101(2):695-696.
[26]	SINGH S,CORNILESCU C C,TYLER R C,et al.Solution structure of a late embryogenesis abundant protein(LEA14)from Arabidopsis thaliana,a cellular stress-related protein[J].Plant Science,2005,14:2601-2609.
[27]	NAOT D,BEN-HAYYIM G,ESHDAT Y,et al.Drought,heat and salt stress induce the expression of a citrus homologue of and atypical late-embryogenesis LEA5gene[J].Plant Molecular Biology,1995,27:619-622.
[28]	ZHAO P,LIU F,ZHENG G,et al.Group3late embryogenesis abundant protein in Arabidopsis:structure,regulation,and function[J].Acta Physiologiae Plantarum,2011,33:1063-1073.
[29]	WOLKERS W F,MCCREADY S,BRANDT W F,et al.Isolation and characterization of a D-7LEA protein from pollen that stabilizes glasses in vitro[J].Biochimicaet Biophysica Acta,2001,1544:196-206.
[30]	BOUCHER V,BUITINK J,LIN X,et al.MtPM25is an atypical hydrophobic late embryogenesis abundant protein that disso-ciates cold and desiccation-aggregated proteins[J].Plant Cell and Environment,2010,33:418-430.
[31]	GILLES G J,HINES K M,MANFRE A J,et al.A predicted N-terminal helical domain of a Group1LEA protein is required for protection of enzyme activity from drying[J].Plant Physiology and Biochemistry,2007,45:389-399.
[32]	LIU X,WANG Z,WANG L,et al.LEA4group genes from the resurrection plant Boeahygrometrica confer dehydration toler-ance in transgenic tobacco[J].Plant Science,2009,176:90-98.
[33]	NAKAYAMA K,OKAWA K,KAKIZAKI T,et al.Evaluation of the protective activities of a late embryogenesis abundant(LEA)related protein,cor15am,during various stresses in vitro[J].Bioscience Biotechnology and Biochemistry,2008,72:1642-1645.
[34]	VEERANAGAMALLAIAH G,PRASANTHI J,REDDY K E.Group1and2LEA protein expression correlates with a de-crease in water stress induced protein aggregation in horsegram during germination and seedling growth[J].Journal of Plant Physiology,2011,168:671-677.
[35]	SUTTON F,DING X,KENEFICK D G.Group3LEA gene HVA1regulation by cold acclimation and deacclimation in two barley cultivars with varying freeze resistance[J].Plant Physiology,1992,99:338-340.
[36]	ALSHEIKH M K,HEYEN B J,RANDALL S K.Ion binding properties of the dehydrin erd14are dependent upon phospho-rylation[J].The Journal of Biological Chemistry,2003,17:40882-40889.
[37]	KRGER C,BERKOWITZ O,STEPHAN U W,et al.A metal-binding member of the late embryogenesis abundant protein family transports iron in the phloem of Ricinus communis L.[J].The Journal of Biological Chemistry,2002,12:25062-25069.
[38]	LIU G B,XU H,ZHANG L,et al.Fe binding properties of two soybean(Glycine max L.)LEA4proteins associated with antioxidant activity[J].Plant and Cell Physiology,2011,56:994-1002.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Structure and Function of LEA Proteins in Higher Plants

Centre for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China

Received Date: 2012-02-20

Key words:

Abstract: Late embryogenesis abundant proteins（LEA proteins） are a group of stress-responsive proteins in higher plants that are induced by environmental stress.These proteins can enhance plant tolerance against environmental stress via stabilizing cell membrane and binding ions.The category and structure of LEA proteins are summarized herein.The researches conducted in recent years are reviewed about the roles of LEA proteins in plant response to drought,low temperature and other environmental stresses.

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Reference (38)

[1]	DURE III L,GRENWAY S C,GALAU G A.Developmental biochemistry of cottonseed embryogenesis and germination:chan-ging messenger ribonucleic acid populations as shown by in vitro and in vivo protein synthesis[J].Biochemistry,1981,20:4162-4168.
[2]	GALAU G A,HUGHES D W,DURE III L.Abscisic acid induction of cloned late embryogenesis-abundant(LEA)mRNAs[J].Plant Molecular Biology,1986,7:155-170.
[3]	WISE M J.LEAping to conclusions:a computational reanalysis of late embryogenesis abundant proteins and their possible roles[J].BMC Bioinformatics,2003,4:52.
[4]	ZEGZOUTI H,JONES B,MARTY C,et al.ER5,a tomato cDNA encoding an ethylene-responsive LEA-like protein:charac-terization and expression in response to drought,ABA and wounding[J].Plant Molecular Biology,1997,35:847-854.
[5]	PARK J A,CHO S K,KIM J E,et al.Isolation of cDNAs differentially expressed in response to drought stress and character-ization of the Ca-LEAL1gene encoding a new family of atypical LEA-like protein homologue in hot pepper(Capsicum annuum L.cv.Pukang)[J].Plant Science,2003,165:471-481.
[6]	RAYNAL M,GAUBIER P,GRELLET F,et al.Nucleotide sequence of a radish cDNA clone coding for a late embryogenesis abundant(LEA)protein[J].Nucleic Acids Research,1900,18:20.
[7]	TUNNACLIFFE A,WISE M J.The continuing conundrum of the LEA proteins[J].Naturwissenschaften,2007,94:791-812.
[8]	于丽霞,汤晓倩,武晓璐,等.茭白lEA3基因全长cDNA的分离克隆及序列分析[J].中国蔬菜,2010(6):14-18.
[9]	刘金仙,阙友雄,郭晋隆,等.甘蔗胚胎晚期丰富蛋白(LEA)cDNA全长克隆及表达特性[J].农业生物技术学报,2009,17(5):836-842.
[10]	MAITRA N,CUSHMAN J C.Isolation and characterization of a drought-induced soybean cDNA encoding a D95famile late-embryogenesis-abundant protein[J].Plant physiology,1994,106:805-806.
[11]	TOLLETER D,HINCHA D K,MACHEREL D.A mitochondrial late embryogenesis abundant protein stabilizes model mem-branes in the dry state[J].Biochimica et Biophysica Acta,2010,1798:1926-1933.
[12]	GRELET J,BENAMAR A,TEYSSIER E,et al.Identification in pea seed mitochondria of a late-embryogenesis abundant protein able to protect enzymes from drying[J].Plant Physiology,2005,137:157-167.
[13]	GOYAL K,WALTON L J,Tunnacliffe A.LEA proteins prevent protein aggregation due to water stress[J].Journal of Bio-chemistry,2005,388:151-157.
[14]	BAKER J,STEELE C,DURE III L.Sequence and characterization of6LEA proteins and their genes from cotton[J].Plant Molecular Biology,1988,11:277-291.
[15]	DURE III L,CROUCH M,HARADA J,et al.Common amino acid sequence domains among the LEA proteins of higher plants[J].Plant Molecular Biology,1989,12:475-486.
[16]	BRAY E A.Molecular responses to water deficit[J].Plant Physiology,1993,103(4):1035-1040.
[17]	HUNDERMARK M,HINCHA D K.LEA(late embryogenesis abundant)proteins and their encoding genes in Arabidopsis thaliana[J].BMC Genomics,2008,9:118.
[18]	RORAT T.Plant dehydrins-tissue location,structure and function[J].Cellular&Molecular Biology Letters,2006,11:536-556.
[19]	VASEVA I I,GRIGOROVA B S,SIMOVA-STOILOVA L P,et al.Abscisic acid and late embryogenesis abundant protein profile changes in winter wheat under progressive drought stress[J].Plant Biology,2010,12:698-707.
[20]	KOAG M C,FENTON R D,WILKENS S,et al.The binding of maize DHN1to lipid vesicles.gain of structure and lipid specificity[J].Plant Physiology,2003,131:309-316.
[21]	ALI-BENALI M A,ALARY R,JOUDRIER P,et al.comparative expression of five Lea genes during wheat seed develop-ment and in response to abiotic stress by real-time quantitative RT-PCR[J].Biochimicaet Biophysica Acta,2005,1730:56-65.
[22]	BRINI F,SAIBI W,AMARA I,et al.Wheat dehydrin DHN-5exerts a heat-protective effect onβ-glucosidase and glucose oxidase activities[J].Bioscience Biotechnology and Biochemistry,2010,74:1050-1054.
[23]	SHAO H B,LIANG Z S,SHAO M A.LEA proteins in higher plants:Structure,function,gene expression and regulation[J].Colloids and Surfaces B:Biointerfaces,2005,45:131-135.
[24]	OLVERA-CARRILLO Y,CAMPOS F,REYES J L,et al.Functional analysis of the group4late embryogenesis abundant proteins reveals their relevance in the adaptive response during water deficit in Arabidopsis[J].Plant Physiology,2010,154:373-390.
[25]	GALAU G A,WANG H Y C,HUGHES D W.Cotton LEA5and LEA14encode atypical late embryogenesis-abundant proteins[J].Plant Physiology,1993,101(2):695-696.
[26]	SINGH S,CORNILESCU C C,TYLER R C,et al.Solution structure of a late embryogenesis abundant protein(LEA14)from Arabidopsis thaliana,a cellular stress-related protein[J].Plant Science,2005,14:2601-2609.
[27]	NAOT D,BEN-HAYYIM G,ESHDAT Y,et al.Drought,heat and salt stress induce the expression of a citrus homologue of and atypical late-embryogenesis LEA5gene[J].Plant Molecular Biology,1995,27:619-622.
[28]	ZHAO P,LIU F,ZHENG G,et al.Group3late embryogenesis abundant protein in Arabidopsis:structure,regulation,and function[J].Acta Physiologiae Plantarum,2011,33:1063-1073.
[29]	WOLKERS W F,MCCREADY S,BRANDT W F,et al.Isolation and characterization of a D-7LEA protein from pollen that stabilizes glasses in vitro[J].Biochimicaet Biophysica Acta,2001,1544:196-206.
[30]	BOUCHER V,BUITINK J,LIN X,et al.MtPM25is an atypical hydrophobic late embryogenesis abundant protein that disso-ciates cold and desiccation-aggregated proteins[J].Plant Cell and Environment,2010,33:418-430.
[31]	GILLES G J,HINES K M,MANFRE A J,et al.A predicted N-terminal helical domain of a Group1LEA protein is required for protection of enzyme activity from drying[J].Plant Physiology and Biochemistry,2007,45:389-399.
[32]	LIU X,WANG Z,WANG L,et al.LEA4group genes from the resurrection plant Boeahygrometrica confer dehydration toler-ance in transgenic tobacco[J].Plant Science,2009,176:90-98.
[33]	NAKAYAMA K,OKAWA K,KAKIZAKI T,et al.Evaluation of the protective activities of a late embryogenesis abundant(LEA)related protein,cor15am,during various stresses in vitro[J].Bioscience Biotechnology and Biochemistry,2008,72:1642-1645.
[34]	VEERANAGAMALLAIAH G,PRASANTHI J,REDDY K E.Group1and2LEA protein expression correlates with a de-crease in water stress induced protein aggregation in horsegram during germination and seedling growth[J].Journal of Plant Physiology,2011,168:671-677.
[35]	SUTTON F,DING X,KENEFICK D G.Group3LEA gene HVA1regulation by cold acclimation and deacclimation in two barley cultivars with varying freeze resistance[J].Plant Physiology,1992,99:338-340.
[36]	ALSHEIKH M K,HEYEN B J,RANDALL S K.Ion binding properties of the dehydrin erd14are dependent upon phospho-rylation[J].The Journal of Biological Chemistry,2003,17:40882-40889.
[37]	KRGER C,BERKOWITZ O,STEPHAN U W,et al.A metal-binding member of the late embryogenesis abundant protein family transports iron in the phloem of Ricinus communis L.[J].The Journal of Biological Chemistry,2002,12:25062-25069.
[38]	LIU G B,XU H,ZHANG L,et al.Fe binding properties of two soybean(Glycine max L.)LEA4proteins associated with antioxidant activity[J].Plant and Cell Physiology,2011,56:994-1002.

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Structure and Function of LEA Proteins in Higher Plants

doi: 10.15886/j.cnki.rdswxb.2012.02.005

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

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