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Volume 1 Issue 3
Sep.  2010
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Article Navigation >  Journal of Tropical Biology  > 2010  >  1(3): 206-209

XIA Zhi-hui, DONG Jun-mei, LUO Yue-hua. Construction of T Vector Using Restriction Endonuclease XcmⅠ[J]. Journal of Tropical Biology, 2010, 1(3): 206-209. doi: 10.15886/j.cnki.rdswxb.2010.03.002
Citation: XIA Zhi-hui, DONG Jun-mei, LUO Yue-hua. Construction of T Vector Using Restriction Endonuclease XcmⅠ[J]. Journal of Tropical Biology, 2010, 1(3): 206-209. doi: 10.15886/j.cnki.rdswxb.2010.03.002
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Construction of T Vector Using Restriction Endonuclease XcmⅠ

doi: 10.15886/j.cnki.rdswxb.2010.03.002

  • College of Agriculture, Hainan University, Haikou, 570228, China

  • Received Date: 2010-07-13
  • In our report,rice variety Nipponbare genome were used as template,a pair of primers which were added a XcmⅠ site at each 5’ end were used to amplify,and a 627bp fragments were obtained,which was ligated into pGEM-T Easy to develop a recombinant vector,T-xia. Self-made T vector was obtained when T-xia was digested by XcmⅠ,and the results of the ligation with other PCR products indicated that 80% recombination clones contained target fragments.
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Construction of T Vector Using Restriction Endonuclease XcmⅠ

doi: 10.15886/j.cnki.rdswxb.2010.03.002
  • College of Agriculture, Hainan University, Haikou, 570228, China
  • Received Date: 2010-07-13

Abstract: In our report,rice variety Nipponbare genome were used as template,a pair of primers which were added a XcmⅠ site at each 5’ end were used to amplify,and a 627bp fragments were obtained,which was ligated into pGEM-T Easy to develop a recombinant vector,T-xia. Self-made T vector was obtained when T-xia was digested by XcmⅠ,and the results of the ligation with other PCR products indicated that 80% recombination clones contained target fragments.

XIA Zhi-hui, DONG Jun-mei, LUO Yue-hua. Construction of T Vector Using Restriction Endonuclease XcmⅠ[J]. Journal of Tropical Biology, 2010, 1(3): 206-209. doi: 10.15886/j.cnki.rdswxb.2010.03.002
Citation: XIA Zhi-hui, DONG Jun-mei, LUO Yue-hua. Construction of T Vector Using Restriction Endonuclease XcmⅠ[J]. Journal of Tropical Biology, 2010, 1(3): 206-209. doi: 10.15886/j.cnki.rdswxb.2010.03.002
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