Genome-wide identification and expression-profiling analysis of the antimicrobial peptide genes in <i>Bactrocera dorsalis</i>（Hendel）

ZHANG Xiaofeng; SU Yu; DONG Xinyi; LIU Yueru; ZHOU Hongxu; FAN Yinjun

doi:10.15886/j.cnki.rdswxb.20240130

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Volume 15 Issue 6

Nov. 2024

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Article Navigation > Journal of Tropical Biology > 2024 > 15(6): 672-682

ZHANG Xiaofeng, SU Yu, DONG Xinyi, LIU Yueru, ZHOU Hongxu, FAN Yinjun. Genome-wide identification and expression-profiling analysis of the antimicrobial peptide genes in Bactrocera dorsalis（Hendel）[J]. Journal of Tropical Biology, 2024, 15(6): 672-682. doi: 10.15886/j.cnki.rdswxb.20240130

Citation:

ZHANG Xiaofeng, SU Yu, DONG Xinyi, LIU Yueru, ZHOU Hongxu, FAN Yinjun. Genome-wide identification and expression-profiling analysis of the antimicrobial peptide genes in Bactrocera dorsalis（Hendel）[J]. Journal of Tropical Biology, 2024, 15(6): 672-682. doi: 10.15886/j.cnki.rdswxb.20240130

Genome-wide identification and expression-profiling analysis of the antimicrobial peptide genes in Bactrocera dorsalis（Hendel）

doi: 10.15886/j.cnki.rdswxb.20240130

College of Plant Health and Medicine, Qingdao Agricultural University/China-Australia Joint Institute of Agricultural and Environmental health, Qingdao, Shandong 266109, China

Received Date: 2024-08-12
Rev Recd Date: 2024-08-25

Abstract

The antimicrobial peptide（AMP） genes of Bactrocera dorsalis were identified at the genome level to explore potential AMPs for the control of B. dorsalis. The composition, structural features, and phylogenetic relationships of AMPs across different insect species were analyzed by using bioinformatics methods based on chromosome-level genomic data. Furthermore, expression profiles of AMPs at different developmental stages in six adult parts were examined by employing qRT-PCRe. A total of 29 AMP genes were identified, including 12cecropins, 11 defensins, 4 attacins, and 2 diptericins. The genomic and protein structures indicate that B. dorsalis AMP molecules and structures exhibit genetic diversity mechanisms, including gene duplication. The expression profiles of AMPs at different developmental stages revealed that most AMPs are highly expressed during the pupal and adult stages. The expression characteristics of different types of AMPs varied significantly in different body parts, and apart from the hemolymph, individual genes were also expressed in the head and integument.These findings provide a database and information for further research on the functions of B. dorsalis AMPs.
- Bactrocera dorsalis,
- antimicrobial peptides,
- cercropin,
- defensin,
- attacin,
- diptericin,
- expression profiling

References

[1]	BASSET Y, CIZEK L, CUÉNOUD P, et al. Arthropod diversity in a tropical forest[J]. Science, 2012, 338(6113):1481-1484.
[2]	BUONOCORE F, FAUSTO A M, DELLA PELLE G, et al.Attacins:a promising class of insect antimicrobial peptides[J]. Antibiotics, 2021, 10(2):212.
[3]	WU Q, PATOČKA J, KUČA K. Insect antimicrobial peptides, a mini review[J]. Toxins, 2018, 10(11):461.
[4]	HANSON M A, LEMAITRE B, UNCKLESS R L. Dynamic evolution of antimicrobial peptides underscores trade-offs between immunity and ecological fitness[J]. Frontiers in Immunology, 2019, 10:2620.
[5]	MANNIELLO M D, MORETTA A, SALVIA R, et al.Insect antimicrobial peptides:potential weapons to counteract the antibiotic resistance[J]. Cellular and Molecular Life Sciences, 2021, 78(9):4259-4282.
[6]	STEINER H, HULTMARK D, ENGSTRÖMÅ, et al.Sequence and specificity of two antibacterial proteins involved in insect immunity[J]. Nature, 1981, 292:246-248.
[7]	HE Y, CAO X, LI K, et al. A genome-wide analysis of antimicrobial effector genes and their transcription patterns in Manduca sexta[J]. Insect Biochemistry and Molecular Biology, 2015, 62:23-37.
[8]	BUONOCORE F, FAUSTO A M, DELLA PELLE G, et al.Attacins:a promising class of insect antimicrobial peptides[J]. Antibiotics, 2021, 10(2):212.
[9]	YI H Y, CHOWDHURY M, HUANG Y D, et al. Insect antimicrobial peptides and their applications[J]. Applied Microbiology and Biotechnology, 2014, 98(13):5807-5822.
[10]	CLARKE A R, ARMSTRONG K F, CARMICHAEL A E,et al. Invasive phytophagous pests arising through a recent tropical evolutionary radiation:the Bactrocera dorsalis complex of fruit flies[J]. Annual Review of Entomology, 2005, 50:293-319.
[11]	张杰,张艳,刘伟,等.橘小实蝇化学通讯机制与引诱剂开发策略[J].昆虫学报, 2023, 66(1):108-120.
[12]	ZHAO Z, CAREY J R, LI Z. The global epidemic of Bactrocera pests:mixed-species invasions and risk assessment[J].Annual Review of Entomology, 2024, 69:219-237.
[13]	NUGNES F, RUSSO E, VIGGIANI G, et al. First record of an invasive fruit fly belonging to Bactrocera dorsalis complex (Diptera:Tephritidae) in Europe[J]. Insects,2018, 9(4):182.
[14]	ZHU Y F, TAN X M, QI F J, et al. The host shift of Bactrocera dorsalis:early warning of the risk of damage to the fruit industry in Northern China[J]. Entomologia Generalis, 2022, 42(5):691-699.
[15]	JIANG F, LIANG L, WANG J, et al. Chromosome-level genome assembly of Bactrocera dorsalis reveals its adaptation and invasion mechanisms[J]. Communications Biology, 2022, 5:25.
[16]	DANG X L, TIAN J H, YANG W Y, et al. Bactrocerin-1:a novel inducible antimicrobial peptide from pupae of oriental fruit fly Bactrocera dorsalis Hendel[J]. Archives of Insect Biochemistry and Physiology, 2009, 71(3):117-129.
[17]	LIAO Y Y, ZUO Y H, TSAI C L, et al. Cdna cloning and transcriptional regulation of the cecropin and attacin from the oriental fruit fly, bactrocera dorsalis (Diptera:Tephritidae)[J]. Archives of Insect Biochemistry and Physiology, 2015, 89(2):111-126.
[18]	LIU S H, WEI D, YUAN G R, et al. Antimicrobial peptide gene cecropin-2 and defensin respond to peptidoglycan infection in the female adult of oriental fruit fly, Bactrocera dorsalis (Hendel)[J]. Comparative Biochemistry and Physiology Part B, Biochemistry&Molecular Biology, 2017, 206:1-7.
[19]	PETERSEN T N, BRUNAK S, VON HEIJNE G, et al. Signal P 4.0:discriminating signal peptides from transmembrane regions[J]. Nature Methods, 2011, 8:785-786.
[20]	QI S, GAO B, ZHU S. Molecular diversity and evolution of antimicrobial peptides in Musca domestica[J]. Diversity, 2021, 13(3):107.
[21]	ELEFTHERIANOS I, ZHANG W, HERYANTO C, et al.Diversity of insect antimicrobial peptides and proteins-A functional perspective:a review[J]. International Journal of Biological Macromolecules, 2021, 191:277-287.
[22]	YASHWANT R S, THOMAS D S, MANOHARAN C, et al. Inducible overexpression of cecropin B decreases the susceptibility of the transgenic silkworm, Bombyx mori (Lepidoptera:Bombycidae), to bacteria[J]. Applied Entomology and Zoology, 2023, 58(1):85-92.
[23]	YI H Y, CHOWDHURY M, HUANG Y D, et al. Insect antimicrobial peptides and their applications[J]. Applied Microbiology and Biotechnology, 2014, 98(13):5807-5822.
[24]	KIM S R, HONG M Y, PARK S W, et al. Characterization and cDNA cloning of a cecropin-like antimicrobial peptide, papiliocin, from the swallowtail butterfly,Papilio xuthus[J]. Molecules and Cells, 2010, 29(4):419-423.
[25]	VIZIOLI J, BULET P, CHARLET M, et al. Cloning and analysis of a cecropin gene from the malaria vector mosquito, Anopheles gambiae[J]. Insect Molecular Biology,2000, 9(1):75-84.
[26]	GUO L, TANG M, LUO S, et al. Screening and functional analyses of novel cecropins from insect transcriptome[J].Insects, 2023, 14(10):794.
[27]	QUESADA H, RAMOS-ONSINS S E, AGUADÉM.Birth-and-death evolution of the cecropin multigene family in Drosophila[J]. Journal of Molecular Evolution,2005, 60(1):1-11.
[28]	WU Q, PATOČKA J, KUČA K. Insect antimicrobial peptides, a mini review[J]. Toxins, 2018, 10(11):461.
[29]	WEN H, LAN X, CHENG T, et al. Sequence structure and expression pattern of a novel anionic defensin-like gene from silkworm (Bombyx mori)[J]. Molecular Biology Reports, 2009, 36(4):711-716.
[30]	DIMARCQ J L, HOFFMANN D, MEISTER M, et al.Characterization and transcriptional profiles of a Drosophila gene encoding an insect defensin. A study in insect immunity[J]. European Journal of Biochemistry,1994, 221(1):201-209.
[31]	SUGIYAMA M, KUNIYOSHI H, KOTANI E, et al. Characterization of a Bombyx mori cDNA encoding a novel member of the attacin family of insect antibacterial proteins[J]. Insect Biochemistry and Molecular Biology,1995, 25(3):385-392.
[32]	GUNNE H, HELLERS M, STEINER H. Structure of preproattacin and its processing in insect cells infected with a recombinant baculovirus[J]. European Journal of Biochemistry, 1990, 187(3):699-703.
[33]	KESHAVARZ M, ZANCHI C, ROLFF J. The effect of combined knockdowns of Attacins on survival and bacterial load in Tenebrio molitor[J]. Frontiers in Immunology,2023, 14:1140627.
[34]	DIMARCQ J L, KEPPI E, DUNBAR B, et al. Insect immunity. Purification and characterization of a family of novel inducible antibacterial proteins from immunized larvae of the dipteran Phormia terranovae and complete amino-acid sequence of the predominant member, diptericin A[J]. European Journal of Biochemistry, 1988,171(1/2):17-22.
[35]	WICKER C, REICHHART J M, HOFFMANN D, et al.Insect immunity. Characterization of a Drosophila cDNA encoding a novel member of the diptericin family of immune peptides[J]. The Journal of Biological Chemistry, 1990, 265(36):22493-22498.
[36]	ISHIKAWA M, KUBO T, NATORI S. Purification and characterization of a diptericin homologue from Sarcophaga peregrina (flesh fly)[J]. The Biochemical Journal, 1992, 287(Pt 2):573-578.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Genome-wide identification and expression-profiling analysis of the antimicrobial peptide genes in Bactrocera dorsalis（Hendel）

College of Plant Health and Medicine, Qingdao Agricultural University/China-Australia Joint Institute of Agricultural and Environmental health, Qingdao, Shandong 266109, China

Received Date: 2024-08-12

Rev Recd Date: 2024-08-25

Key words:

Abstract: The antimicrobial peptide（AMP） genes of Bactrocera dorsalis were identified at the genome level to explore potential AMPs for the control of B. dorsalis. The composition, structural features, and phylogenetic relationships of AMPs across different insect species were analyzed by using bioinformatics methods based on chromosome-level genomic data. Furthermore, expression profiles of AMPs at different developmental stages in six adult parts were examined by employing qRT-PCRe. A total of 29 AMP genes were identified, including 12cecropins, 11 defensins, 4 attacins, and 2 diptericins. The genomic and protein structures indicate that B. dorsalis AMP molecules and structures exhibit genetic diversity mechanisms, including gene duplication. The expression profiles of AMPs at different developmental stages revealed that most AMPs are highly expressed during the pupal and adult stages. The expression characteristics of different types of AMPs varied significantly in different body parts, and apart from the hemolymph, individual genes were also expressed in the head and integument.These findings provide a database and information for further research on the functions of B. dorsalis AMPs.

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Reference (36)

[1]	BASSET Y, CIZEK L, CUÉNOUD P, et al. Arthropod diversity in a tropical forest[J]. Science, 2012, 338(6113):1481-1484.
[2]	BUONOCORE F, FAUSTO A M, DELLA PELLE G, et al.Attacins:a promising class of insect antimicrobial peptides[J]. Antibiotics, 2021, 10(2):212.
[3]	WU Q, PATOČKA J, KUČA K. Insect antimicrobial peptides, a mini review[J]. Toxins, 2018, 10(11):461.
[4]	HANSON M A, LEMAITRE B, UNCKLESS R L. Dynamic evolution of antimicrobial peptides underscores trade-offs between immunity and ecological fitness[J]. Frontiers in Immunology, 2019, 10:2620.
[5]	MANNIELLO M D, MORETTA A, SALVIA R, et al.Insect antimicrobial peptides:potential weapons to counteract the antibiotic resistance[J]. Cellular and Molecular Life Sciences, 2021, 78(9):4259-4282.
[6]	STEINER H, HULTMARK D, ENGSTRÖMÅ, et al.Sequence and specificity of two antibacterial proteins involved in insect immunity[J]. Nature, 1981, 292:246-248.
[7]	HE Y, CAO X, LI K, et al. A genome-wide analysis of antimicrobial effector genes and their transcription patterns in Manduca sexta[J]. Insect Biochemistry and Molecular Biology, 2015, 62:23-37.
[8]	BUONOCORE F, FAUSTO A M, DELLA PELLE G, et al.Attacins:a promising class of insect antimicrobial peptides[J]. Antibiotics, 2021, 10(2):212.
[9]	YI H Y, CHOWDHURY M, HUANG Y D, et al. Insect antimicrobial peptides and their applications[J]. Applied Microbiology and Biotechnology, 2014, 98(13):5807-5822.
[10]	CLARKE A R, ARMSTRONG K F, CARMICHAEL A E,et al. Invasive phytophagous pests arising through a recent tropical evolutionary radiation:the Bactrocera dorsalis complex of fruit flies[J]. Annual Review of Entomology, 2005, 50:293-319.
[11]	张杰,张艳,刘伟,等.橘小实蝇化学通讯机制与引诱剂开发策略[J].昆虫学报, 2023, 66(1):108-120.
[12]	ZHAO Z, CAREY J R, LI Z. The global epidemic of Bactrocera pests:mixed-species invasions and risk assessment[J].Annual Review of Entomology, 2024, 69:219-237.
[13]	NUGNES F, RUSSO E, VIGGIANI G, et al. First record of an invasive fruit fly belonging to Bactrocera dorsalis complex (Diptera:Tephritidae) in Europe[J]. Insects,2018, 9(4):182.
[14]	ZHU Y F, TAN X M, QI F J, et al. The host shift of Bactrocera dorsalis:early warning of the risk of damage to the fruit industry in Northern China[J]. Entomologia Generalis, 2022, 42(5):691-699.
[15]	JIANG F, LIANG L, WANG J, et al. Chromosome-level genome assembly of Bactrocera dorsalis reveals its adaptation and invasion mechanisms[J]. Communications Biology, 2022, 5:25.
[16]	DANG X L, TIAN J H, YANG W Y, et al. Bactrocerin-1:a novel inducible antimicrobial peptide from pupae of oriental fruit fly Bactrocera dorsalis Hendel[J]. Archives of Insect Biochemistry and Physiology, 2009, 71(3):117-129.
[17]	LIAO Y Y, ZUO Y H, TSAI C L, et al. Cdna cloning and transcriptional regulation of the cecropin and attacin from the oriental fruit fly, bactrocera dorsalis (Diptera:Tephritidae)[J]. Archives of Insect Biochemistry and Physiology, 2015, 89(2):111-126.
[18]	LIU S H, WEI D, YUAN G R, et al. Antimicrobial peptide gene cecropin-2 and defensin respond to peptidoglycan infection in the female adult of oriental fruit fly, Bactrocera dorsalis (Hendel)[J]. Comparative Biochemistry and Physiology Part B, Biochemistry&Molecular Biology, 2017, 206:1-7.
[19]	PETERSEN T N, BRUNAK S, VON HEIJNE G, et al. Signal P 4.0:discriminating signal peptides from transmembrane regions[J]. Nature Methods, 2011, 8:785-786.
[20]	QI S, GAO B, ZHU S. Molecular diversity and evolution of antimicrobial peptides in Musca domestica[J]. Diversity, 2021, 13(3):107.
[21]	ELEFTHERIANOS I, ZHANG W, HERYANTO C, et al.Diversity of insect antimicrobial peptides and proteins-A functional perspective:a review[J]. International Journal of Biological Macromolecules, 2021, 191:277-287.
[22]	YASHWANT R S, THOMAS D S, MANOHARAN C, et al. Inducible overexpression of cecropin B decreases the susceptibility of the transgenic silkworm, Bombyx mori (Lepidoptera:Bombycidae), to bacteria[J]. Applied Entomology and Zoology, 2023, 58(1):85-92.
[23]	YI H Y, CHOWDHURY M, HUANG Y D, et al. Insect antimicrobial peptides and their applications[J]. Applied Microbiology and Biotechnology, 2014, 98(13):5807-5822.
[24]	KIM S R, HONG M Y, PARK S W, et al. Characterization and cDNA cloning of a cecropin-like antimicrobial peptide, papiliocin, from the swallowtail butterfly,Papilio xuthus[J]. Molecules and Cells, 2010, 29(4):419-423.
[25]	VIZIOLI J, BULET P, CHARLET M, et al. Cloning and analysis of a cecropin gene from the malaria vector mosquito, Anopheles gambiae[J]. Insect Molecular Biology,2000, 9(1):75-84.
[26]	GUO L, TANG M, LUO S, et al. Screening and functional analyses of novel cecropins from insect transcriptome[J].Insects, 2023, 14(10):794.
[27]	QUESADA H, RAMOS-ONSINS S E, AGUADÉM.Birth-and-death evolution of the cecropin multigene family in Drosophila[J]. Journal of Molecular Evolution,2005, 60(1):1-11.
[28]	WU Q, PATOČKA J, KUČA K. Insect antimicrobial peptides, a mini review[J]. Toxins, 2018, 10(11):461.
[29]	WEN H, LAN X, CHENG T, et al. Sequence structure and expression pattern of a novel anionic defensin-like gene from silkworm (Bombyx mori)[J]. Molecular Biology Reports, 2009, 36(4):711-716.
[30]	DIMARCQ J L, HOFFMANN D, MEISTER M, et al.Characterization and transcriptional profiles of a Drosophila gene encoding an insect defensin. A study in insect immunity[J]. European Journal of Biochemistry,1994, 221(1):201-209.
[31]	SUGIYAMA M, KUNIYOSHI H, KOTANI E, et al. Characterization of a Bombyx mori cDNA encoding a novel member of the attacin family of insect antibacterial proteins[J]. Insect Biochemistry and Molecular Biology,1995, 25(3):385-392.
[32]	GUNNE H, HELLERS M, STEINER H. Structure of preproattacin and its processing in insect cells infected with a recombinant baculovirus[J]. European Journal of Biochemistry, 1990, 187(3):699-703.
[33]	KESHAVARZ M, ZANCHI C, ROLFF J. The effect of combined knockdowns of Attacins on survival and bacterial load in Tenebrio molitor[J]. Frontiers in Immunology,2023, 14:1140627.
[34]	DIMARCQ J L, KEPPI E, DUNBAR B, et al. Insect immunity. Purification and characterization of a family of novel inducible antibacterial proteins from immunized larvae of the dipteran Phormia terranovae and complete amino-acid sequence of the predominant member, diptericin A[J]. European Journal of Biochemistry, 1988,171(1/2):17-22.
[35]	WICKER C, REICHHART J M, HOFFMANN D, et al.Insect immunity. Characterization of a Drosophila cDNA encoding a novel member of the diptericin family of immune peptides[J]. The Journal of Biological Chemistry, 1990, 265(36):22493-22498.
[36]	ISHIKAWA M, KUBO T, NATORI S. Purification and characterization of a diptericin homologue from Sarcophaga peregrina (flesh fly)[J]. The Biochemical Journal, 1992, 287(Pt 2):573-578.

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Genome-wide identification and expression-profiling analysis of the antimicrobial peptide genes in Bactrocera dorsalis（Hendel）

doi: 10.15886/j.cnki.rdswxb.20240130

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

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Genome-wide identification and expression-profiling analysis of the antimicrobial peptide genes in Bactrocera dorsalis（Hendel）

doi: 10.15886/j.cnki.rdswxb.20240130

College of Plant Health and Medicine, Qingdao Agricultural University/China-Australia Joint Institute of Agricultural and Environmental health, Qingdao, Shandong 266109, China

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Genome-wide identification and expression-profiling analysis of the antimicrobial peptide genes in Bactrocera dorsalis（Hendel）

doi: 10.15886/j.cnki.rdswxb.20240130

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

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Genome-wide identification and expression-profiling analysis of the antimicrobial peptide genes in Bactrocera dorsalis（Hendel）

doi: 10.15886/j.cnki.rdswxb.20240130

College of Plant Health and Medicine, Qingdao Agricultural University/China-Australia Joint Institute of Agricultural and Environmental health, Qingdao, Shandong 266109, China

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