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Volume 15 Issue 6
Nov.  2024
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Article Contents
Article Navigation >  Journal of Tropical Biology  > 2024  >  15(6): 756-763

WU Haoheng, LIU Zhu, SHO Jia, MA Xiang, LI Hong, TANG Yanqiong, CHI Xue, LI Juanjuan. Evaluation of the therapeutic effect of Bacillus subtilis on the intestinal tract of mice infected by Aeromonas veronii[J]. Journal of Tropical Biology, 2024, 15(6): 756-763. doi: 10.15886/j.cnki.rdswxb.20240112
Citation: WU Haoheng, LIU Zhu, SHO Jia, MA Xiang, LI Hong, TANG Yanqiong, CHI Xue, LI Juanjuan. Evaluation of the therapeutic effect of Bacillus subtilis on the intestinal tract of mice infected by Aeromonas veronii[J]. Journal of Tropical Biology, 2024, 15(6): 756-763. doi: 10.15886/j.cnki.rdswxb.20240112
shu

Evaluation of the therapeutic effect of Bacillus subtilis on the intestinal tract of mice infected by Aeromonas veronii

doi: 10.15886/j.cnki.rdswxb.20240112

  • School of Life and Health Sciences, Hainan University, Haikou, Hainan 570228, China

  • Received Date: 2024-06-29
  • Rev Recd Date: 2024-08-20
  • Infection of Aeromonas veronii（A. veronii）, a kind of zoonotic multidrug-resistant bacteria, is mainly treated or prevented with antibiotics. However, the extensive use of antibiotics can lead to the emergence of new drug-resistant mutant strains. Bacillus subtilis（B. subtilis）, as a common probiotic, which can inhibit various species of pathogenic bacteria. The antibacterial ability of B. subtilis against A. veronii was detected and its therapeutic effect on A. veronii intestinal infection evaluated. Filter paper method showed that B. subtilis possessed an obvious inhibitory effect on A. veronii C4, and TEM showed that it caused the dilatation and damage of A. veronii C4 cells. In addition, B. subtilis reduced the intracellular cytotoxicity of A. veronii C4. In a mouse model with damaged intestinal barrier, B. subtilis showed higher efficiency than the clinical antibiotic on A.veronii C4 infections, contributing to their natural ability of intestinal barrier repair and the antibacterial ability against A. veronii C4. B.subtilis can thus be used as a biocontrol agent for the treatment of A. veronii infection,which is of great significance for curbing the development of drug-resistance.
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Evaluation of the therapeutic effect of Bacillus subtilis on the intestinal tract of mice infected by Aeromonas veronii

doi: 10.15886/j.cnki.rdswxb.20240112
  • School of Life and Health Sciences, Hainan University, Haikou, Hainan 570228, China
  • Received Date: 2024-06-29
  • Rev Recd Date: 2024-08-20

Abstract: Infection of Aeromonas veronii（A. veronii）, a kind of zoonotic multidrug-resistant bacteria, is mainly treated or prevented with antibiotics. However, the extensive use of antibiotics can lead to the emergence of new drug-resistant mutant strains. Bacillus subtilis（B. subtilis）, as a common probiotic, which can inhibit various species of pathogenic bacteria. The antibacterial ability of B. subtilis against A. veronii was detected and its therapeutic effect on A. veronii intestinal infection evaluated. Filter paper method showed that B. subtilis possessed an obvious inhibitory effect on A. veronii C4, and TEM showed that it caused the dilatation and damage of A. veronii C4 cells. In addition, B. subtilis reduced the intracellular cytotoxicity of A. veronii C4. In a mouse model with damaged intestinal barrier, B. subtilis showed higher efficiency than the clinical antibiotic on A.veronii C4 infections, contributing to their natural ability of intestinal barrier repair and the antibacterial ability against A. veronii C4. B.subtilis can thus be used as a biocontrol agent for the treatment of A. veronii infection,which is of great significance for curbing the development of drug-resistance.

WU Haoheng, LIU Zhu, SHO Jia, MA Xiang, LI Hong, TANG Yanqiong, CHI Xue, LI Juanjuan. Evaluation of the therapeutic effect of Bacillus subtilis on the intestinal tract of mice infected by Aeromonas veronii[J]. Journal of Tropical Biology, 2024, 15(6): 756-763. doi: 10.15886/j.cnki.rdswxb.20240112
Citation: WU Haoheng, LIU Zhu, SHO Jia, MA Xiang, LI Hong, TANG Yanqiong, CHI Xue, LI Juanjuan. Evaluation of the therapeutic effect of Bacillus subtilis on the intestinal tract of mice infected by Aeromonas veronii[J]. Journal of Tropical Biology, 2024, 15(6): 756-763. doi: 10.15886/j.cnki.rdswxb.20240112
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