Genome-wide analysis of <i>HbCCoAOMT</i> gene family in rubber tree

ZHANG Qianyu; YUAN Hongmei

doi:10.15886/j.cnki.rdswxb.20240093

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To further understand the role of the HbCCoAOMT gene in the growth, development, and stress resistance of the rubber tree（Hevea brasiliensis）, the HbCCoAOMT family genes were identified and analyzed by examining the whole genome sequencing data of H. brasiliensis. A total of 40 members of the HbCCoAOMT family were identified, and their distribution on chromosomes, domain characteristics, and tissue expression profiles were analyzed. The results revealed that HbCCoAOMT members were only distributed in 7 chromosomes: 1, 3, 4,11, 13, 17, and 18 out of the18 chromosones in rubber trees. Expression pattern analysis indicated that 12members were expressed in all the five tissues. The HbCCoAOMT enzyme activity assay showed the enzyme activity was highest in the stems of H. brasiliensis. Moreover, HbCCoAOMT1, which had the highest expression abundance, was successfully cloned from H. brasiliensis. The CDS length of HbCCoAOMT1 is 746 bp, encoding 241 amino acids. HbCCoAOMT1 was inserted into the prokaryotic expression vector p ET28a, and the recombinant HbCCoAOMT1 protein was successfully expressed in E. coli BL21（DE3）. Analysis of the HbCCoAOMT1 promoter region showed that HbCCoAOMT1 contains various hormone-and stress-related cisacting elements.

Genome-wide analysis of HbCCoAOMT gene family in rubber tree

School of Breeding and Multiplication/Sanya Institute of Breeding and Multiplication, Hainan University, Sanya, Hainan 572025, China

Received Date: 2024-06-05

Rev Recd Date: 2024-06-30

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Abstract: To further understand the role of the HbCCoAOMT gene in the growth, development, and stress resistance of the rubber tree（Hevea brasiliensis）, the HbCCoAOMT family genes were identified and analyzed by examining the whole genome sequencing data of H. brasiliensis. A total of 40 members of the HbCCoAOMT family were identified, and their distribution on chromosomes, domain characteristics, and tissue expression profiles were analyzed. The results revealed that HbCCoAOMT members were only distributed in 7 chromosomes: 1, 3, 4,11, 13, 17, and 18 out of the18 chromosones in rubber trees. Expression pattern analysis indicated that 12members were expressed in all the five tissues. The HbCCoAOMT enzyme activity assay showed the enzyme activity was highest in the stems of H. brasiliensis. Moreover, HbCCoAOMT1, which had the highest expression abundance, was successfully cloned from H. brasiliensis. The CDS length of HbCCoAOMT1 is 746 bp, encoding 241 amino acids. HbCCoAOMT1 was inserted into the prokaryotic expression vector p ET28a, and the recombinant HbCCoAOMT1 protein was successfully expressed in E. coli BL21（DE3）. Analysis of the HbCCoAOMT1 promoter region showed that HbCCoAOMT1 contains various hormone-and stress-related cisacting elements.

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Genome-wide analysis of HbCCoAOMT gene family in rubber tree

doi: 10.15886/j.cnki.rdswxb.20240093

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Genome-wide analysis of HbCCoAOMT gene family in rubber tree

doi: 10.15886/j.cnki.rdswxb.20240093

School of Breeding and Multiplication/Sanya Institute of Breeding and Multiplication, Hainan University, Sanya, Hainan 572025, China

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Genome-wide analysis of HbCCoAOMT gene family in rubber tree

doi: 10.15886/j.cnki.rdswxb.20240093

Abstract

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通讯作者: 陈斌, bchen63@163.com

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Genome-wide analysis of HbCCoAOMT gene family in rubber tree

doi: 10.15886/j.cnki.rdswxb.20240093

School of Breeding and Multiplication/Sanya Institute of Breeding and Multiplication, Hainan University, Sanya, Hainan 572025, China

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