Rapid propagation of rubber tree by using intermittent immersion Bioreactor

DONG Lihao; CHEN Jianmiao; WANG Kaixuan; GUO Jiaxin

doi:10.15886/j.cnki.rdswxb.20240068

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The integument somatic embryos of rubber tree of clone RRIM 600 were cultured in an intermittent immersion bioreactor RITA^®, and the suitable conditions for somatic embryo culture of rubber tree were established. The types of hormones were screened and the culture conditions were optimized. The experimental results showed that the best condition for intermittent immersion culture of RRIM 600 rubber integument primordium-derived somatic embryos were: MS + 1.0 mg·L^-1 6-BA + 0.1 mg·L^-1 NAA + 50 g·L^-1 sucrose as the medium, with the intermittent immersion frequency of nutrient solution being 1 min/6 h, and the inoculation density being 15 individual embryos/container. Compared with solid culture, the intermittent immersion culture under this condition increased significantly the plant height, stem diameter, root length, root number and fresh weight per plant of somatic embryo regeneration plants by 30%, 91.7%, 34.8%, 53.3% and 97.2%, respectively.The coordination ability of SOD, CAT and POD in regenerated plants was higher under this condition, which was an effective way to improve the efficiency of regeneration plant formation from primary somatic embryos of rubber tree. All these results showed that the use of intermittent immersion culture method RITA^® could shorten the formation cycle of somatic embryo regeneration plants and improve the growth quality of somatic embryo plants of rubber tree.

Rapid propagation of rubber tree by using intermittent immersion Bioreactor

1. School of Breeding and Multiplication/Sanya Institute of Breeding and Multiplication, Hainan University, Sanya, Hainan 572024;

2. School of Tropical Agriculture and Forestry, Danzhou, Hainan 571737, China

Received Date: 2024-04-18

Rev Recd Date: 2024-05-29

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Abstract: The integument somatic embryos of rubber tree of clone RRIM 600 were cultured in an intermittent immersion bioreactor RITA^®, and the suitable conditions for somatic embryo culture of rubber tree were established. The types of hormones were screened and the culture conditions were optimized. The experimental results showed that the best condition for intermittent immersion culture of RRIM 600 rubber integument primordium-derived somatic embryos were: MS + 1.0 mg·L^-1 6-BA + 0.1 mg·L^-1 NAA + 50 g·L^-1 sucrose as the medium, with the intermittent immersion frequency of nutrient solution being 1 min/6 h, and the inoculation density being 15 individual embryos/container. Compared with solid culture, the intermittent immersion culture under this condition increased significantly the plant height, stem diameter, root length, root number and fresh weight per plant of somatic embryo regeneration plants by 30%, 91.7%, 34.8%, 53.3% and 97.2%, respectively.The coordination ability of SOD, CAT and POD in regenerated plants was higher under this condition, which was an effective way to improve the efficiency of regeneration plant formation from primary somatic embryos of rubber tree. All these results showed that the use of intermittent immersion culture method RITA^® could shorten the formation cycle of somatic embryo regeneration plants and improve the growth quality of somatic embryo plants of rubber tree.

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Rapid propagation of rubber tree by using intermittent immersion Bioreactor

doi: 10.15886/j.cnki.rdswxb.20240068 cstr: 32425.14.j.cnki.rdswxb.20240068

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