Characteristics of Eukaryotic Microbial Communities on the Macrofouler Initial Attachment Areas at the Aquaculture Region in Qinglan Port

CHEN Yingjie; CHEN Xin; LI Jiancong; LIU Zongyue; TANG Min

doi:10.15886/j.cnki.rdswxb.20240027

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Volume 15 Issue 6

Nov. 2024

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Article Navigation > Journal of Tropical Biology > 2024 > 15(6): 791-799

CHEN Yingjie, CHEN Xin, LI Jiancong, LIU Zongyue, TANG Min. Characteristics of Eukaryotic Microbial Communities on the Macrofouler Initial Attachment Areas at the Aquaculture Region in Qinglan Port[J]. Journal of Tropical Biology, 2024, 15(6): 791-799. doi: 10.15886/j.cnki.rdswxb.20240027

Citation:

CHEN Yingjie, CHEN Xin, LI Jiancong, LIU Zongyue, TANG Min. Characteristics of Eukaryotic Microbial Communities on the Macrofouler Initial Attachment Areas at the Aquaculture Region in Qinglan Port[J]. Journal of Tropical Biology, 2024, 15(6): 791-799. doi: 10.15886/j.cnki.rdswxb.20240027

Characteristics of Eukaryotic Microbial Communities on the Macrofouler Initial Attachment Areas at the Aquaculture Region in Qinglan Port

doi: 10.15886/j.cnki.rdswxb.20240027

1. School of Ecology and Environment, Hainan University, Haikou, Hainan 570228, China;
2. School of Physics and Optoelectronic Engineering, Hainan University, Haikou, Hainan 570228, China

Received Date: 2024-02-09
Rev Recd Date: 2024-03-14

Abstract

In the nearshore mariculture area in Qinglan Port, Hainan, three materials, polyethylene（PE）,polyamide（PA）, and polymethyl methacrylate（PMMA）, were employed as sea-hanging panels in the mariculture area and microbial biofilms were collected from the macrofoulers on the panels for high-throughput sequencing and analysis. A comparative analysis of the characteristics of eukaryotic microbial communities in the initial attachment areas（IAA） of three macrofouler, including Hydroides elegans, Balanus amphitrite and Membranipora savartii, showed that the microbial community structures in the initial attachment areas of the same species of a macrofouler on the three materials exhibited high similarity. The dominant taxa in the eukaryotic microbial communities varied among different macrofouler IAAs, with Ulva（43.02%-93.31%） dominating in H. elegans,Picochlorum（10.74%-60.86%） in B. amphitrite, and Monhystrella（12.08%-34.78%） in M. savartii. The alpha diversity of the eukaryotic microbial community in M. savartii IAA was the highest. Co-occurrence network analysis indicated that mutualistic symbiosis was the predominant interspecies relationship in the eukaryotic microbial community. Functional prediction analysis revealed higher abundances of taxa associated with biosynthesis, degradation/utilization/assimilation, generation of precursor metabolite and energy production in the eukaryotic microbial communities of IAAs. This study contributes to a better understanding of the correlation between the attachment of large fouling animals and the underlying microbial community, providing reference data for anti-fouling work in mariculture.
- macrofouler,
- initial attachment areas,
- eukaryotic microbial communities,
- Qinglan Port

References

[1]	HADFIELD M G. Biofilms and marine invertebrate larvae:what bacteria produce that larvae use to choose settlement sites[J]. Annual Review of Marine Science, 2011, 3:453-470.
[2]	HUANG S, HADFIELD M G. Composition and density of bacterial biofilms determine larval settlement of the polychaete Hydroides elegans[J]. Marine Ecology Progress Series, 2003, 260:161-172.
[3]	周轩,郭行磐,陈芋如,等.低湿度表面的海洋附着细菌对厚壳贻贝附着的影响[J].大连海洋大学学报, 2015,30(1):30-35.
[4]	SHIKUMA N J, PILHOFER M, WEISS G L, et al. Marine tubeworm metamorphosis induced by arrays of bacterial phage tail-like structures[J]. Science, 2014, 343(6170):529-533.
[5]	TAIT K, HAVENHAND J. Investigating a possible role for the bacterial signal molecules N-acylhomoserine lactones in Balanus improvisus cyprid settlement[J]. Molecular Ecology, 2013, 22(9):2588-2602.
[6]	LEE J W, NAM J H, KIM Y H, et al. Bacterial communities in the initial stage of marine biofilm formation on artificial surfaces[J]. The Journal of Microbiology, 2008,46(2):174-182.
[7]	QIAN P Y, LAU S C K, DAHMS H U, et al. Marine biofilms as mediators of colonization by marine macroorganisms:implications for antifouling and aquaculture[J].Marine Biotechnology, 2007, 9(4):399-410.
[8]	DOBRETSOV S, RITTSCHOF D. Love at first taste:induction of larval settlement by marine microbes[J]. International Journal of Molecular Sciences, 2020, 21(3):731.
[9]	LEE O O, CHUNG H C, YANG J, et al. Molecular techniques revealed highly diverse microbial communities in natural marine biofilms on polystyrene dishes for invertebrate larval settlement[J]. Microbial Ecology, 2014,68(1):81-93.
[10]	LIANG X, PENG L H, ZHANG S, et al. Polyurethane,epoxy resin and polydimethylsiloxane altered biofilm formation and mussel settlement[J]. Chemosphere, 2019,218:599-608.
[11]	刘倩倩,史宏伟,郭长禄,等.海鞘附着相关微生物膜中细菌原核群落结构解析[J].生物技术通报, 2020,36(11):76-84.
[12]	SUN Y, LANG Y, PAN Y, et al. Colonization characteristics of pioneer surface-associated eukaryotes during natural biofilm formation on PDMS-based composites via 18S rRNA gene sequencing methods[J]. International Biodeterioration&Biodegradation, 2022, 166:105341.
[13]	石建高,余雯雯,赵奎,等.海水网箱网衣防污技术的研究进展[J].水产学报, 2021, 45(3):472-485.
[14]	王越,张敏,石建高,等.渔用防污材料的研究进展及其在渔业上的应用[J].海洋渔业, 2021, 43(2):247-256.
[15]	黄宗国,林茂.中国海洋生物图集-第四册, 2-动物界[M].北京:海洋出版社, 2012.
[16]	刘勐伶,严涛.南海污损生物生态研究进展[J].海洋通报, 2006, 25(1):84-91.
[17]	陈明华,谢良国,付志强,等.丙酮法和热乙醇法测定浮游植物叶绿素a的方法比对[J].环境监测管理与技术, 2016, 28(2):46-48.
[18]	ZHANG B, YANG X, LIU L, et al. Spatial and seasonal variations in biofilm formation on microplastics in coastal waters[J]. The Science of the Total Environment, 2021,770:145303.
[19]	SALTA M, WHARTON J A, BLACHE Y, et al. Marine biofilms on artificial surfaces:structure and dynamics[J].Environmental Microbiology, 2013, 15(11):2879-2893.
[20]	VON AMMON U, WOOD S A, LAROCHE O, et al. The impact of artificial surfaces on marine bacterial and eukaryotic biofouling assemblages:a high-throughput sequencing analysis[J]. Marine Environmental Research,2018, 133:57-66.
[21]	JOUUCHI T, SATUITO C G, KITAMURA H. Sugar compound products of the periphytic diatom Navicula ramosissima induce larval settlement in the barnacle,Amphibalanus amphitrite[J]. Marine Biology, 2007,152(5):1065-1076.
[22]	QIAN P Y, CHENG A, WANG R, et al. Marine biofilms:diversity, interactions and biofouling[J]. Nature Reviews Microbiology, 2022, 20:671-684.
[23]	JIN C, QIU J, YU S, et al. Histamine promotes the larval metamorphic competence of barnacle Amphibalanus amphitrite[J]. Marine Biology Research, 2014, 10(8):799-806.
[24]	张娜.纹藤壶幼虫发育与信号物质关系研究[D].扬州:扬州大学, 2018.
[25]	SHI X, WANG Y Q, YANG Y M, et al. Knockdown of two iodothyronine deiodinase genes inhibits epinephrineinduced larval metamorphosis of the hard-shelled mussel Mytilus coruscus[J]. Frontiers in Marine Science, 2022,9:914283.
[26]	LAGOS M E, WHITE C R, MARSHALL D J. Avoiding low-oxygen environments:oxytaxis as a mechanism of habitat selection in a marine invertebrate[J]. Marine Ecology Progress Series, 2015, 540:99-107.
[27]	CHEUNG S G, CHAN C Y S, PO B H K, et al. Effects of hypoxia on biofilms and subsequently larval settlement of benthic invertebrates[J]. Marine Pollution Bulletin,2014, 85(2):418-424.
[28]	DAHMS H U, DOBRETSOV S, QIAN P Y. The effect of bacterial and diatom biofilms on the settlement of the bryozoan Bugula neritina[J]. Journal of Experimental Marine Biology and Ecology, 2004, 313(1):191-209.
[29]	DOBRETSOV S, QIAN P Y. Facilitation and inhibition of larval attachment of the bryozoan Bugula neritina in association with mono-species and multi-species biofilms[J].Journal of Experimental Marine Biology and Ecology,2006, 333(2):263-274.
[30]	COYTE K Z, SCHLUTER J, FOSTER K R. The ecology of the microbiome:networks, competition, and stability[J].Science, 2015, 350(6261):663-666.
[31]	STEELE J A, COUNTWAY P D, XIA L, et al. Marine bacterial, archaeal and protistan association networks reveal ecological linkages[J]. The ISME Journal, 2011,5(9):1414-1425.
[32]	EILER A, HEINRICH F, BERTILSSON S. Coherent dynamics and association networks among lake bacterioplankton taxa[J]. The ISME Journal, 2012, 6(2):330-342.
[33]	BANERJEE S, SCHLAEPPI K, VAN DER HEIJDEN M G A. Keystone taxa as drivers of microbiome structure and functioning[J]. Nature Reviews Microbiology, 2018,16:567-576.
[34]	GORTER F A, MANHART M, ACKERMANN M. Understanding the evolution of interspecies interactions in microbial communities[J]. Philosophical Transactions of the Royal Society of London Series B, Biological Sciences, 2020, 375(1798):20190256.
[35]	CHIU J M Y, THIYAGARAJAN V, TSOI M M Y, et al.Qualitative and quantitative changes in marine biofilms as a function of temperature and salinity in summer and winter[J]. Biofilms, 2005, 2(3):183-195.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Characteristics of Eukaryotic Microbial Communities on the Macrofouler Initial Attachment Areas at the Aquaculture Region in Qinglan Port

1. School of Ecology and Environment, Hainan University, Haikou, Hainan 570228, China;

2. School of Physics and Optoelectronic Engineering, Hainan University, Haikou, Hainan 570228, China

Received Date: 2024-02-09

Rev Recd Date: 2024-03-14

Key words:

Abstract: In the nearshore mariculture area in Qinglan Port, Hainan, three materials, polyethylene（PE）,polyamide（PA）, and polymethyl methacrylate（PMMA）, were employed as sea-hanging panels in the mariculture area and microbial biofilms were collected from the macrofoulers on the panels for high-throughput sequencing and analysis. A comparative analysis of the characteristics of eukaryotic microbial communities in the initial attachment areas（IAA） of three macrofouler, including Hydroides elegans, Balanus amphitrite and Membranipora savartii, showed that the microbial community structures in the initial attachment areas of the same species of a macrofouler on the three materials exhibited high similarity. The dominant taxa in the eukaryotic microbial communities varied among different macrofouler IAAs, with Ulva（43.02%-93.31%） dominating in H. elegans,Picochlorum（10.74%-60.86%） in B. amphitrite, and Monhystrella（12.08%-34.78%） in M. savartii. The alpha diversity of the eukaryotic microbial community in M. savartii IAA was the highest. Co-occurrence network analysis indicated that mutualistic symbiosis was the predominant interspecies relationship in the eukaryotic microbial community. Functional prediction analysis revealed higher abundances of taxa associated with biosynthesis, degradation/utilization/assimilation, generation of precursor metabolite and energy production in the eukaryotic microbial communities of IAAs. This study contributes to a better understanding of the correlation between the attachment of large fouling animals and the underlying microbial community, providing reference data for anti-fouling work in mariculture.

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[1]	HADFIELD M G. Biofilms and marine invertebrate larvae:what bacteria produce that larvae use to choose settlement sites[J]. Annual Review of Marine Science, 2011, 3:453-470.
[2]	HUANG S, HADFIELD M G. Composition and density of bacterial biofilms determine larval settlement of the polychaete Hydroides elegans[J]. Marine Ecology Progress Series, 2003, 260:161-172.
[3]	周轩,郭行磐,陈芋如,等.低湿度表面的海洋附着细菌对厚壳贻贝附着的影响[J].大连海洋大学学报, 2015,30(1):30-35.
[4]	SHIKUMA N J, PILHOFER M, WEISS G L, et al. Marine tubeworm metamorphosis induced by arrays of bacterial phage tail-like structures[J]. Science, 2014, 343(6170):529-533.
[5]	TAIT K, HAVENHAND J. Investigating a possible role for the bacterial signal molecules N-acylhomoserine lactones in Balanus improvisus cyprid settlement[J]. Molecular Ecology, 2013, 22(9):2588-2602.
[6]	LEE J W, NAM J H, KIM Y H, et al. Bacterial communities in the initial stage of marine biofilm formation on artificial surfaces[J]. The Journal of Microbiology, 2008,46(2):174-182.
[7]	QIAN P Y, LAU S C K, DAHMS H U, et al. Marine biofilms as mediators of colonization by marine macroorganisms:implications for antifouling and aquaculture[J].Marine Biotechnology, 2007, 9(4):399-410.
[8]	DOBRETSOV S, RITTSCHOF D. Love at first taste:induction of larval settlement by marine microbes[J]. International Journal of Molecular Sciences, 2020, 21(3):731.
[9]	LEE O O, CHUNG H C, YANG J, et al. Molecular techniques revealed highly diverse microbial communities in natural marine biofilms on polystyrene dishes for invertebrate larval settlement[J]. Microbial Ecology, 2014,68(1):81-93.
[10]	LIANG X, PENG L H, ZHANG S, et al. Polyurethane,epoxy resin and polydimethylsiloxane altered biofilm formation and mussel settlement[J]. Chemosphere, 2019,218:599-608.
[11]	刘倩倩,史宏伟,郭长禄,等.海鞘附着相关微生物膜中细菌原核群落结构解析[J].生物技术通报, 2020,36(11):76-84.
[12]	SUN Y, LANG Y, PAN Y, et al. Colonization characteristics of pioneer surface-associated eukaryotes during natural biofilm formation on PDMS-based composites via 18S rRNA gene sequencing methods[J]. International Biodeterioration&Biodegradation, 2022, 166:105341.
[13]	石建高,余雯雯,赵奎,等.海水网箱网衣防污技术的研究进展[J].水产学报, 2021, 45(3):472-485.
[14]	王越,张敏,石建高,等.渔用防污材料的研究进展及其在渔业上的应用[J].海洋渔业, 2021, 43(2):247-256.
[15]	黄宗国,林茂.中国海洋生物图集-第四册, 2-动物界[M].北京:海洋出版社, 2012.
[16]	刘勐伶,严涛.南海污损生物生态研究进展[J].海洋通报, 2006, 25(1):84-91.
[17]	陈明华,谢良国,付志强,等.丙酮法和热乙醇法测定浮游植物叶绿素a的方法比对[J].环境监测管理与技术, 2016, 28(2):46-48.
[18]	ZHANG B, YANG X, LIU L, et al. Spatial and seasonal variations in biofilm formation on microplastics in coastal waters[J]. The Science of the Total Environment, 2021,770:145303.
[19]	SALTA M, WHARTON J A, BLACHE Y, et al. Marine biofilms on artificial surfaces:structure and dynamics[J].Environmental Microbiology, 2013, 15(11):2879-2893.
[20]	VON AMMON U, WOOD S A, LAROCHE O, et al. The impact of artificial surfaces on marine bacterial and eukaryotic biofouling assemblages:a high-throughput sequencing analysis[J]. Marine Environmental Research,2018, 133:57-66.
[21]	JOUUCHI T, SATUITO C G, KITAMURA H. Sugar compound products of the periphytic diatom Navicula ramosissima induce larval settlement in the barnacle,Amphibalanus amphitrite[J]. Marine Biology, 2007,152(5):1065-1076.
[22]	QIAN P Y, CHENG A, WANG R, et al. Marine biofilms:diversity, interactions and biofouling[J]. Nature Reviews Microbiology, 2022, 20:671-684.
[23]	JIN C, QIU J, YU S, et al. Histamine promotes the larval metamorphic competence of barnacle Amphibalanus amphitrite[J]. Marine Biology Research, 2014, 10(8):799-806.
[24]	张娜.纹藤壶幼虫发育与信号物质关系研究[D].扬州:扬州大学, 2018.
[25]	SHI X, WANG Y Q, YANG Y M, et al. Knockdown of two iodothyronine deiodinase genes inhibits epinephrineinduced larval metamorphosis of the hard-shelled mussel Mytilus coruscus[J]. Frontiers in Marine Science, 2022,9:914283.
[26]	LAGOS M E, WHITE C R, MARSHALL D J. Avoiding low-oxygen environments:oxytaxis as a mechanism of habitat selection in a marine invertebrate[J]. Marine Ecology Progress Series, 2015, 540:99-107.
[27]	CHEUNG S G, CHAN C Y S, PO B H K, et al. Effects of hypoxia on biofilms and subsequently larval settlement of benthic invertebrates[J]. Marine Pollution Bulletin,2014, 85(2):418-424.
[28]	DAHMS H U, DOBRETSOV S, QIAN P Y. The effect of bacterial and diatom biofilms on the settlement of the bryozoan Bugula neritina[J]. Journal of Experimental Marine Biology and Ecology, 2004, 313(1):191-209.
[29]	DOBRETSOV S, QIAN P Y. Facilitation and inhibition of larval attachment of the bryozoan Bugula neritina in association with mono-species and multi-species biofilms[J].Journal of Experimental Marine Biology and Ecology,2006, 333(2):263-274.
[30]	COYTE K Z, SCHLUTER J, FOSTER K R. The ecology of the microbiome:networks, competition, and stability[J].Science, 2015, 350(6261):663-666.
[31]	STEELE J A, COUNTWAY P D, XIA L, et al. Marine bacterial, archaeal and protistan association networks reveal ecological linkages[J]. The ISME Journal, 2011,5(9):1414-1425.
[32]	EILER A, HEINRICH F, BERTILSSON S. Coherent dynamics and association networks among lake bacterioplankton taxa[J]. The ISME Journal, 2012, 6(2):330-342.
[33]	BANERJEE S, SCHLAEPPI K, VAN DER HEIJDEN M G A. Keystone taxa as drivers of microbiome structure and functioning[J]. Nature Reviews Microbiology, 2018,16:567-576.
[34]	GORTER F A, MANHART M, ACKERMANN M. Understanding the evolution of interspecies interactions in microbial communities[J]. Philosophical Transactions of the Royal Society of London Series B, Biological Sciences, 2020, 375(1798):20190256.
[35]	CHIU J M Y, THIYAGARAJAN V, TSOI M M Y, et al.Qualitative and quantitative changes in marine biofilms as a function of temperature and salinity in summer and winter[J]. Biofilms, 2005, 2(3):183-195.

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Characteristics of Eukaryotic Microbial Communities on the Macrofouler Initial Attachment Areas at the Aquaculture Region in Qinglan Port

doi: 10.15886/j.cnki.rdswxb.20240027

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

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