Effects of different proportions of organic fertilizer replacing chemical nitrogen fertilizer on soil nutrients and enzyme activities of rubber plantations in Hainan Island

LU Wen; XU Wenxian; SU Tianyan; YU Lei; JIANG Yamin; LU Jingli; LIU Wenjie; YANG Qiu

doi:10.15886/j.cnki.rdswxb.20240007

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Volume 15 Issue 6

Nov. 2024

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Article Navigation > Journal of Tropical Biology > 2024 > 15(6): 745-755

LU Wen, XU Wenxian, SU Tianyan, YU Lei, JIANG Yamin, LU Jingli, LIU Wenjie, YANG Qiu. Effects of different proportions of organic fertilizer replacing chemical nitrogen fertilizer on soil nutrients and enzyme activities of rubber plantations in Hainan Island[J]. Journal of Tropical Biology, 2024, 15(6): 745-755. doi: 10.15886/j.cnki.rdswxb.20240007

Citation:

LU Wen, XU Wenxian, SU Tianyan, YU Lei, JIANG Yamin, LU Jingli, LIU Wenjie, YANG Qiu. Effects of different proportions of organic fertilizer replacing chemical nitrogen fertilizer on soil nutrients and enzyme activities of rubber plantations in Hainan Island[J]. Journal of Tropical Biology, 2024, 15(6): 745-755. doi: 10.15886/j.cnki.rdswxb.20240007

Effects of different proportions of organic fertilizer replacing chemical nitrogen fertilizer on soil nutrients and enzyme activities of rubber plantations in Hainan Island

doi: 10.15886/j.cnki.rdswxb.20240007

LU Wen^1,2,
XU Wenxian³,
SU Tianyan^1,2,
YU Lei^1,2,
JIANG Yamin^1,2,
LU Jingli^1,2,
LIU Wenjie^1,2,
YANG Qiu^{1,2
,
,}

1. College of Ecology and Environment, Hainan University, Haikou, Hainan 570228, China;
2. Key Laboratory of Agro-Forestry Environmental Processes and Ecological Regulation of Hainan Province, Haikou, Hainan 570228, China;
3. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China

Received Date: 2024-01-11
Rev Recd Date: 2024-03-08

Abstract

The effects of different proportions of organic fertilizer replacing chemical nitrogen fertilizer on soil nutrient and enzyme activity in rubber plantations is of great significance for selecting a reasonable substitution ratio and the sustainable development of natural rubber. The rubber plantations in Zhubijiang Farm, Baisha Li Autonomous County, Hainan Province was selected as the subject, and six different treatments were set up from June 2022 using the principle of equal nitrogen application: no fertilizer treatment（CK）, Conventional fertilizer treatment（NPK）, organic fertilizer replacing 25% chemical nitrogen treatment（25% M）, organic fertilizer replacing 50% chemical nitrogen treatment（50% M）, organic fertilizer replacing 75% chemical nitrogen treatment（75% M）, organic fertilizer replacing 100% chemical nitrogen treatment（100% M）. Soil samples of 0 ～10 cm, 10 ～ 20 cm, 20 ～ 40 cm and 40 ～ 60 cm were collected after fertilization for 4 months, and the characteristics of soil nutrients and enzyme activities were analyzed. The results showed that 1) compared with the NPK treatment, the 25% M and 50% M treatments significantly increased the soil organic carbon（SOC）content in 0 ～ 10 cm, 10 ～ 20 cm, 20 ～ 40 cm and 40 ～ 60 cm soil layers. The 75% M treatment significantly increased soil total N（TN） content in all soil layers at 0 ～ 10 cm and 10 ～ 20 cm. The 75% M and 100% M significantly increased the content of soil nitrate-N（NO₃^--N） in 0 ～ 10 cm soil layer. The 25% M treatment significantly increased the content of soil NH₄⁺-N in 0 ～ 10 cm, 10 ～ 20 cm, and 20 ～ 40 cm soil layers. The 100% M treatment significantly increased the content of soil available P（AvP） in 0 ～ 10 cm and 10 ～ 20 cm soil layers. 2) Compared with the CK treatment, the 75% M treatment significantly increased activities of C（β-1,4-glucosidase, BG）, N（L-leucine aminopeptidase, LAP; β-1, 4-N-Acetyl Glucosaminase（AP））, P（Acid phosphatase, AP） cycling enzymes in the 10 ～ 20 cm and 40 ～ 60 cm soil layers. The pH, SOC, soil water content（SWC）, TN, AvP, NH₄⁺-N and NO₃^--N were the main factors affecting the activities of C, N and P cycle.3) Random forest model showed that compared with the NPK treatment, the 50% M, 75% M and 100% M treatments significantly increased the rubber yield per plant by 11.9%, 16.4% and 11.8%, respectively. In summary, the 75% substitution ratio of organic fertilizer is effective on increasing the soil TN content and soil C,N, and P cycle-related enzyme activity, thereby improving the rubber plantation soil environment, better increasing the individual rubber yield, promoting the sustainable development of rubber production.
- rubbber plantation,
- organic fertilizer replacement,
- soil enzyme activity,
- soil enzyme ecostoichiometry,
- soil nutrients

References

[1]	海南省市场监督管理局、海南省农业农村厅、海南省林业局.《海南省天然橡胶产业标准体系》[EB/OL].海南省市场监督管理局, 2023-08-15.[2023-12 -07] . https://amr. hainan. gov. cn/zw/gsgg/qtgsgg/202308/t20230815_3475479.html.
[2]	王大鹏,罗雪华,郭澎涛,等.橡胶林氮肥施用后在土壤中的残留、累积和迁移[J].生态环境学报, 2019, 28(7):1361-1368.
[3]	CHEN C, LIU W, WU J, et al. Can intercropping with the cash crop help improve the soil physico-chemical properties of rubber plantations?[J]. Geoderma, 2019, 335:149-160.
[4]	张焱华,何鹏,吴敏,等.橡胶园土壤酶活性与土壤肥力的关系[J].中国农学通报, 2007, 23(11):375-379.
[5]	WANG Y, ZHU Y C, ZHANG S X, et al. What could promote farmers to replace chemical fertilizers with organic fertilizers?[J]. Cleaner Production, 2018, 199(20):882-890.
[6]	SALEHI A, FALLAH S, SOURKI A A. Organic and inorganic fertilizer effect on soil CO₂ flux, microbial biomass,and growth of Nigella sativa L[J]. International Agrophysics, 2017, 31(1):103-116.
[7]	SARMA B, FAROOQ M, GOGOI N, et al. Soil organic carbon dynamics in wheat-Green gram crop rotation amended with vermicompost and biochar in combination with inorganic fertilizers:a comparative study[J]. Journal of Cleaner Production, 2018, 201:471-480.
[8]	GUO Z, HAN J, LI J, et al. Correction:effects of long-term fertilization on soil organic carbon mineralization and microbial community structure[J]. PLoS One, 2019, 14(4):e0216006.
[9]	李佳乐.有机肥替代化学氮肥(尿素)对橡胶幼苗生长和胶园土壤碳氮环境的影响[D].海口:海南大学,2022.
[10]	李更新,马夙静,李祥英,等.有机肥施用对烟田土壤理化性质的影响[J].农业研究与应用, 2018, 31(6):5-10.
[11]	CUI Y, FANG L, GUO X, et al. Ecoenzymatic stoichiometry and microbial nutrient limitation in rhizosphere soil in the arid area of the northern Loess Plateau, China[J].Soil Biology and Biochemistry, 2018, 116:11-21.
[12]	CUI Y, BING H, FANG L, et al. Diversity patterns of the rhizosphere and bulk soil microbial communities along an altitudinal gradient in an alpine ecosystem of the eastern Tibetan Plateau[J]. Geoderma, 2019, 338:118-127.
[13]	TAPIA-TORRES Y, ELSER J J, SOUZA V, et al. Ecoenzymatic stoichiometry at the extremes:how microbes cope in an ultra-oligotrophic desert soil[J]. Soil Biology and Biochemistry, 2015, 87:34-42.
[14]	闫本帅,孙利鹏,李晶晶,等.辽东栎林次生演替过程中土壤酶化学计量特征变化[J].生态学杂志, 2022,41(4):641-647.
[15]	SINSABAUGH R L, LAUBER C L, WEINTRAUB M N, et al. Stoichiometry of soil enzyme activity at global scale[J].Ecology Letters, 2008, 11(11):1252-1264.
[16]	吕来新,宋蕾,刘志理,等.红松人工林土壤酶活性与化学性质对氮添加的响应[J].环境科学, 2020, 41(4):1960-1967.
[17]	余高,陈芬,谢英荷,等.有机肥替代化肥比例对黄壤土活性有机碳及酶活性的影响[J].中国蔬菜, 2020(4):48-55.
[18]	吕凤莲,侯苗苗,张弘弢,等.塿土冬小麦-夏玉米轮作体系有机肥替代化肥比例研究[J].植物营养与肥料学报, 2018, 24(1):22-32.
[19]	徐文娴.绿肥还田及有机肥替代化学氮肥对橡胶园土壤养分的影响研究[D].海口:海南大学, 2022.
[20]	鲍士旦.土壤农化分析[M]. 3版.北京:中国农业出版社, 2000:1-97.
[21]	TONG X, XU M, WANG X, et al. Long-term fertilization effects on organic carbon fractions in a red soil of China[J].CATENA, 2014, 113:251-259.
[22]	杨晓东,孙萍,胡涛,等.有机肥部分替代氮肥对南粳9108产量及土壤理化现状的影响[J].浙江农业科学,2023, 64(3):559-562.
[23]	吴志祥,谢贵水,陶忠良,等.海南儋州不同林龄橡胶林土壤碳和全氮特征[J].生态环境学报, 2009, 18(4):1484-1491.
[24]	SHAO X F, XU M G, ZHANG W J, et al. Changes of soil carbon and nitrogen and characteristics of nitrogen mineralization under long-term manure fertilization practices in black soil[J]. Journal of Plant Nutrition and Fertilizer,2014, 20:326-335.
[25]	原寒,徐子雯,岑宇,等.不同有机肥对土壤养分及大蒜生长发育的影响[J].山东农业科学, 2019, 51(7):60-66.
[26]	WEI M, HU G, WANG H, et al. 35 years of manure and chemical fertilizer application alters soil microbial community composition in a Fluvo-aquic soil in Northern China[J]. European Journal of Soil Biology, 2017, 82:27-34.
[27]	LIU J, SHU A, SONG W, et al. Long-term organic fertilizer substitution increases rice yield by improving soil properties and regulating soil bacteria[J]. Geoderma,2021, 404:115287.
[28]	黄尚书,江新凤,朱同,等.有机肥全量替代化肥对茶园土壤机械稳定团聚体无机氮分布特征的影响[J].华北农学报, 2023, 38(4):141-151.
[29]
[30]	符学知,魏孔亮,罗微,等.有机肥替代化肥处理对橡胶开割树生长状况的影响[J].特种经济动植物, 2022,25(1):23-28.
[31]	DHAKAL Y, MEENA R S, KUMAR S. Effect of INM on nodulation, yield, quality and available nutrient status in soil after harvest of greengram[J]. Legume Research-An International Journal, 2016, 39(4):590-594.
[32]	何进勤,雷金银,韩乃荣,等.牛粪不同处理配施化肥对旱地土壤养分及玉米生物性状的影响[J].天津农业科学, 2021, 27(2):73-76.
[33]	巩庆利,翟丙年,郑伟,等.渭北旱地苹果园生草覆盖下不同肥料配施对土壤养分和酶活性的影响[J].应用生态学报, 2018, 29(1):205-212.
[34]	KAUTZ T, LÓPEZ-FANDO C, ELLMER F. Abundance and biodiversity of soil microarthropods as influenced by different types of organic manure in a long-term field experiment in Central Spain[J]. Applied Soil Ecology,2006, 33(3):278-285.
[35]	马晓霞,王莲莲,黎青慧,等.长期施肥对玉米生育期土壤微生物量碳氮及酶活性的影响[J].生态学报,2012, 32(17):5502-5511.
[36]	SINSABAUGH R L, HILL B H, FOLLSTAD SHAH J J.Ecoenzymatic stoichiometry of microbial organic nutrient acquisition in soil and sediment[J]. Nature, 2009, 462(7274):795-798.
[37]	侯红乾,刘秀梅,刘光荣,等.有机无机肥配施比例对红壤稻田水稻产量和土壤肥力的影响[J].中国农业科学, 2011, 44(3):516-523.
[38]	罗佳,蒋小芳,孟琳,等.不同堆肥原料的有机无机复合肥对油菜生长及土壤供氮特性的影响[J].土壤学报, 2010, 47(1):97-106.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Effects of different proportions of organic fertilizer replacing chemical nitrogen fertilizer on soil nutrients and enzyme activities of rubber plantations in Hainan Island

1. College of Ecology and Environment, Hainan University, Haikou, Hainan 570228, China;

2. Key Laboratory of Agro-Forestry Environmental Processes and Ecological Regulation of Hainan Province, Haikou, Hainan 570228, China;

3. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China

Received Date: 2024-01-11

Rev Recd Date: 2024-03-08

Key words:

Abstract: The effects of different proportions of organic fertilizer replacing chemical nitrogen fertilizer on soil nutrient and enzyme activity in rubber plantations is of great significance for selecting a reasonable substitution ratio and the sustainable development of natural rubber. The rubber plantations in Zhubijiang Farm, Baisha Li Autonomous County, Hainan Province was selected as the subject, and six different treatments were set up from June 2022 using the principle of equal nitrogen application: no fertilizer treatment（CK）, Conventional fertilizer treatment（NPK）, organic fertilizer replacing 25% chemical nitrogen treatment（25% M）, organic fertilizer replacing 50% chemical nitrogen treatment（50% M）, organic fertilizer replacing 75% chemical nitrogen treatment（75% M）, organic fertilizer replacing 100% chemical nitrogen treatment（100% M）. Soil samples of 0 ～10 cm, 10 ～ 20 cm, 20 ～ 40 cm and 40 ～ 60 cm were collected after fertilization for 4 months, and the characteristics of soil nutrients and enzyme activities were analyzed. The results showed that 1) compared with the NPK treatment, the 25% M and 50% M treatments significantly increased the soil organic carbon（SOC）content in 0 ～ 10 cm, 10 ～ 20 cm, 20 ～ 40 cm and 40 ～ 60 cm soil layers. The 75% M treatment significantly increased soil total N（TN） content in all soil layers at 0 ～ 10 cm and 10 ～ 20 cm. The 75% M and 100% M significantly increased the content of soil nitrate-N（NO₃^--N） in 0 ～ 10 cm soil layer. The 25% M treatment significantly increased the content of soil NH₄⁺-N in 0 ～ 10 cm, 10 ～ 20 cm, and 20 ～ 40 cm soil layers. The 100% M treatment significantly increased the content of soil available P（AvP） in 0 ～ 10 cm and 10 ～ 20 cm soil layers. 2) Compared with the CK treatment, the 75% M treatment significantly increased activities of C（β-1,4-glucosidase, BG）, N（L-leucine aminopeptidase, LAP; β-1, 4-N-Acetyl Glucosaminase（AP））, P（Acid phosphatase, AP） cycling enzymes in the 10 ～ 20 cm and 40 ～ 60 cm soil layers. The pH, SOC, soil water content（SWC）, TN, AvP, NH₄⁺-N and NO₃^--N were the main factors affecting the activities of C, N and P cycle.3) Random forest model showed that compared with the NPK treatment, the 50% M, 75% M and 100% M treatments significantly increased the rubber yield per plant by 11.9%, 16.4% and 11.8%, respectively. In summary, the 75% substitution ratio of organic fertilizer is effective on increasing the soil TN content and soil C,N, and P cycle-related enzyme activity, thereby improving the rubber plantation soil environment, better increasing the individual rubber yield, promoting the sustainable development of rubber production.

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Reference (38)

[1]	海南省市场监督管理局、海南省农业农村厅、海南省林业局.《海南省天然橡胶产业标准体系》[EB/OL].海南省市场监督管理局, 2023-08-15.[2023-12	-07] . https://amr. hainan. gov. cn/zw/gsgg/qtgsgg/202308/t20230815_3475479.html.
[2]	王大鹏,罗雪华,郭澎涛,等.橡胶林氮肥施用后在土壤中的残留、累积和迁移[J].生态环境学报, 2019, 28(7):1361-1368.
[3]	CHEN C, LIU W, WU J, et al. Can intercropping with the cash crop help improve the soil physico-chemical properties of rubber plantations?[J]. Geoderma, 2019, 335:149-160.
[4]	张焱华,何鹏,吴敏,等.橡胶园土壤酶活性与土壤肥力的关系[J].中国农学通报, 2007, 23(11):375-379.
[5]	WANG Y, ZHU Y C, ZHANG S X, et al. What could promote farmers to replace chemical fertilizers with organic fertilizers?[J]. Cleaner Production, 2018, 199(20):882-890.
[6]	SALEHI A, FALLAH S, SOURKI A A. Organic and inorganic fertilizer effect on soil CO₂ flux, microbial biomass,and growth of Nigella sativa L[J]. International Agrophysics, 2017, 31(1):103-116.
[7]	SARMA B, FAROOQ M, GOGOI N, et al. Soil organic carbon dynamics in wheat-Green gram crop rotation amended with vermicompost and biochar in combination with inorganic fertilizers:a comparative study[J]. Journal of Cleaner Production, 2018, 201:471-480.
[8]	GUO Z, HAN J, LI J, et al. Correction:effects of long-term fertilization on soil organic carbon mineralization and microbial community structure[J]. PLoS One, 2019, 14(4):e0216006.
[9]	李佳乐.有机肥替代化学氮肥(尿素)对橡胶幼苗生长和胶园土壤碳氮环境的影响[D].海口:海南大学,2022.
[10]	李更新,马夙静,李祥英,等.有机肥施用对烟田土壤理化性质的影响[J].农业研究与应用, 2018, 31(6):5-10.
[11]	CUI Y, FANG L, GUO X, et al. Ecoenzymatic stoichiometry and microbial nutrient limitation in rhizosphere soil in the arid area of the northern Loess Plateau, China[J].Soil Biology and Biochemistry, 2018, 116:11-21.
[12]	CUI Y, BING H, FANG L, et al. Diversity patterns of the rhizosphere and bulk soil microbial communities along an altitudinal gradient in an alpine ecosystem of the eastern Tibetan Plateau[J]. Geoderma, 2019, 338:118-127.
[13]	TAPIA-TORRES Y, ELSER J J, SOUZA V, et al. Ecoenzymatic stoichiometry at the extremes:how microbes cope in an ultra-oligotrophic desert soil[J]. Soil Biology and Biochemistry, 2015, 87:34-42.
[14]	闫本帅,孙利鹏,李晶晶,等.辽东栎林次生演替过程中土壤酶化学计量特征变化[J].生态学杂志, 2022,41(4):641-647.
[15]	SINSABAUGH R L, LAUBER C L, WEINTRAUB M N, et al. Stoichiometry of soil enzyme activity at global scale[J].Ecology Letters, 2008, 11(11):1252-1264.
[16]	吕来新,宋蕾,刘志理,等.红松人工林土壤酶活性与化学性质对氮添加的响应[J].环境科学, 2020, 41(4):1960-1967.
[17]	余高,陈芬,谢英荷,等.有机肥替代化肥比例对黄壤土活性有机碳及酶活性的影响[J].中国蔬菜, 2020(4):48-55.
[18]	吕凤莲,侯苗苗,张弘弢,等.塿土冬小麦-夏玉米轮作体系有机肥替代化肥比例研究[J].植物营养与肥料学报, 2018, 24(1):22-32.
[19]	徐文娴.绿肥还田及有机肥替代化学氮肥对橡胶园土壤养分的影响研究[D].海口:海南大学, 2022.
[20]	鲍士旦.土壤农化分析[M]. 3版.北京:中国农业出版社, 2000:1-97.
[21]	TONG X, XU M, WANG X, et al. Long-term fertilization effects on organic carbon fractions in a red soil of China[J].CATENA, 2014, 113:251-259.
[22]	杨晓东,孙萍,胡涛,等.有机肥部分替代氮肥对南粳9108产量及土壤理化现状的影响[J].浙江农业科学,2023, 64(3):559-562.
[23]	吴志祥,谢贵水,陶忠良,等.海南儋州不同林龄橡胶林土壤碳和全氮特征[J].生态环境学报, 2009, 18(4):1484-1491.
[24]	SHAO X F, XU M G, ZHANG W J, et al. Changes of soil carbon and nitrogen and characteristics of nitrogen mineralization under long-term manure fertilization practices in black soil[J]. Journal of Plant Nutrition and Fertilizer,2014, 20:326-335.
[25]	原寒,徐子雯,岑宇,等.不同有机肥对土壤养分及大蒜生长发育的影响[J].山东农业科学, 2019, 51(7):60-66.
[26]	WEI M, HU G, WANG H, et al. 35 years of manure and chemical fertilizer application alters soil microbial community composition in a Fluvo-aquic soil in Northern China[J]. European Journal of Soil Biology, 2017, 82:27-34.
[27]	LIU J, SHU A, SONG W, et al. Long-term organic fertilizer substitution increases rice yield by improving soil properties and regulating soil bacteria[J]. Geoderma,2021, 404:115287.
[28]	黄尚书,江新凤,朱同,等.有机肥全量替代化肥对茶园土壤机械稳定团聚体无机氮分布特征的影响[J].华北农学报, 2023, 38(4):141-151.
[29]
[30]	符学知,魏孔亮,罗微,等.有机肥替代化肥处理对橡胶开割树生长状况的影响[J].特种经济动植物, 2022,25(1):23-28.
[31]	DHAKAL Y, MEENA R S, KUMAR S. Effect of INM on nodulation, yield, quality and available nutrient status in soil after harvest of greengram[J]. Legume Research-An International Journal, 2016, 39(4):590-594.
[32]	何进勤,雷金银,韩乃荣,等.牛粪不同处理配施化肥对旱地土壤养分及玉米生物性状的影响[J].天津农业科学, 2021, 27(2):73-76.
[33]	巩庆利,翟丙年,郑伟,等.渭北旱地苹果园生草覆盖下不同肥料配施对土壤养分和酶活性的影响[J].应用生态学报, 2018, 29(1):205-212.
[34]	KAUTZ T, LÓPEZ-FANDO C, ELLMER F. Abundance and biodiversity of soil microarthropods as influenced by different types of organic manure in a long-term field experiment in Central Spain[J]. Applied Soil Ecology,2006, 33(3):278-285.
[35]	马晓霞,王莲莲,黎青慧,等.长期施肥对玉米生育期土壤微生物量碳氮及酶活性的影响[J].生态学报,2012, 32(17):5502-5511.
[36]	SINSABAUGH R L, HILL B H, FOLLSTAD SHAH J J.Ecoenzymatic stoichiometry of microbial organic nutrient acquisition in soil and sediment[J]. Nature, 2009, 462(7274):795-798.
[37]	侯红乾,刘秀梅,刘光荣,等.有机无机肥配施比例对红壤稻田水稻产量和土壤肥力的影响[J].中国农业科学, 2011, 44(3):516-523.
[38]	罗佳,蒋小芳,孟琳,等.不同堆肥原料的有机无机复合肥对油菜生长及土壤供氮特性的影响[J].土壤学报, 2010, 47(1):97-106.

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Effects of different proportions of organic fertilizer replacing chemical nitrogen fertilizer on soil nutrients and enzyme activities of rubber plantations in Hainan Island

doi: 10.15886/j.cnki.rdswxb.20240007

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

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