Molecular detection and analysis of main viruses infecting <i>Passiflora edulis</i> planted in Zhanjiang,Guangdong Province

ZHAO Rui; LUO Qingwen; LAN Jian; CHEN Mengyi; ZHOU Renqiang; ZHENG Zejun; XIE Jiangjiang

doi:10.15886/j.cnki.rdswxb.20230126

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Volume 15 Issue 6

Nov. 2024

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Article Navigation > Journal of Tropical Biology > 2024 > 15(6): 691-699

ZHAO Rui, LUO Qingwen, LAN Jian, CHEN Mengyi, ZHOU Renqiang, ZHENG Zejun, XIE Jiangjiang. Molecular detection and analysis of main viruses infecting Passiflora edulis planted in Zhanjiang,Guangdong Province[J]. Journal of Tropical Biology, 2024, 15(6): 691-699. doi: 10.15886/j.cnki.rdswxb.20230126

Citation:

ZHAO Rui, LUO Qingwen, LAN Jian, CHEN Mengyi, ZHOU Renqiang, ZHENG Zejun, XIE Jiangjiang. Molecular detection and analysis of main viruses infecting Passiflora edulis planted in Zhanjiang,Guangdong Province[J]. Journal of Tropical Biology, 2024, 15(6): 691-699. doi: 10.15886/j.cnki.rdswxb.20230126

Molecular detection and analysis of main viruses infecting Passiflora edulis planted in Zhanjiang,Guangdong Province

doi: 10.15886/j.cnki.rdswxb.20230126

1. Zhanjiang Research Center, Institute of Nanfan & Seed Industry, Guangdong Academy of Sciences, Zhanjiang, Guangdong, 524000, China;
2. Zhanjiang Research Academy, Guangdong Academy of Sciences, Zhanjiang, Guangdong, 524000, China

Received Date: 2023-11-14
Rev Recd Date: 2024-01-14

Abstract

Leaves of virus-free seedlings of Passiflora edulis ‘Qinmi 9’ and suspected diseased P. edulis plants were collected for extraction of nucleic acids. P. edulis virus detection primers were designed according to the conserved regions of P. edulis woodiness virus, P. edulis mottle virus, East Asian Passiflora virus, Telosma mosaic virus, P. edulis leaf distortion virus, Euphorbia leaf curl virus, Papaya leaf curl Guangdong virus and Cucumber mosaic virus., and the virus species in different samples were identified by using PCR method. No suspected virus fragments were amplified from the virus-free seedlings of P. edulis. A total of five possible virus fragments were amplified from suspected infected P. edulis seedling samples in the field. They were identified by sequencing as East Asian P. edulis virus,Telosma mosaic virus and Euphorbia leaf curl virus, Papaya leaf curl Guangdong virus. Sequence alignment and phylogenetic tree analysis showed that the PCR products had a high homology with the virus strains from Taiwan Province of China, the Republic of Korea, Fujian Province of China and other regions. The ‘Qinmi 9’ plant planted locally in Zhanjiang was confirmed to carry the virus EAPV, TeMV, EuLCV, PaLCuGDV. This is also the first time that viruses EAPV, PaLCuGV and EuLCV were detected in P. edulis in Guangdong, China.
- Passiflora caerulea L.,
- Qinmi 9,
- PCR detection,
- phylogenetic analysis

References

[1]	田青兰,吴艳艳,黄伟华,等.'台农1号'西番莲的成花坐果特性及与气象因子的关系[J].果树学报, 2020,37(9):1358-1370.
[2]	徐平东,李梅,柯冲.福建西番莲病毒病的发生及其病原黄瓜花叶病毒亚组鉴定[J].植物保护学报,1999, 26(1):50-54.
[3]	CHANG C A. Characterization and comparison of passionfruit mottle virus, a newly recognized potyvirus, with passionfruit woodiness virus[J]. Phytopathology, 1992, 82(11):1358.
[4]	NASCIMENTO A V S, SANTANA E N, BRAZ A S K, et al. Cowpea aphid-borne mosaic virus (CABMV) is widespread in passionfruit in Brazil and causes passionfruit woodiness disease[J]. Archives of Virology, 2006, 151(9):1797-1809.
[5]	IWAI H, YAMASHITA Y, NISHI N, et al. The potyvirus associated with the dappled fruit of Passiflora edulis in Kagoshima prefecture, Japan is the third strain of the proposed new species East Asian Passiflora virus (EAPV) phylogenetically distinguished from strains of Passion fruit woodiness virus[J]. Archives of Virology, 2006, 151(4):811-818.
[6]	CHIEMSOMBAT P, PRAMMANEE S, PIPATTANAWONG N. Occurrence of Telosma mosaic virus causing passion fruit severe mosaic disease in Thailand and immunostrip test for rapid virus detection[J]. Crop Protection, 2014, 63:41-47.
[7]	CHENG Y H, DENG T C, CHEN C C, et al. First Report of Euphorbia leaf curl virus and Papaya leaf curl Guangdong virus on Passion Fruit in Taiwan[J]. Plant Disease,2014, 98(12):1746.
[8]	宋若楠,侯永翔,崔梦迪,等.广东百香果病毒的检测[J].仲恺农业工程学院学报, 2020, 33(1):11-15.
[9]	谢丽雪,张立杰,张小艳,等.福建西番莲上首次检出东亚西番莲病毒[J].园艺学报, 2018, 45(8):1587-1594.
[10]	谢丽雪,张小艳,郑姗,等.侵染西番莲的夜来香花叶病毒的分子鉴定及特异性检测[J].中国农业科学,2017, 50(24):4725-4734.
[11]	李景远.广东番木瓜曲叶病毒和大戟曲叶病毒的致病性分析[D].福州:福建农林大学, 2022.
[12]	谢慧婷,崔丽贤,李战彪,等.广西百香果病毒病病原种类鉴定研究初报[C]//中国植物病理学会.中国植物病理学会2018年学术年会论文集.中国农业科学技术出版社, 2018:1.
[13]	WANG X, XIE Y, ZHOU X. Molecular characterization of two distinct begomovirusesfrom Papaya in China[J].Virus Genes, 2004, 29(3):303-309.
[14]	VACA-VACA J C, CARRASCO-LOZANO E C, LÓPEZLÓPEZ K. Molecular identification of a new begomovirus infecting yellow passion fruit (Passiflora edulis) in Colombia[J]. Archives of Virology, 2017, 162(2):573-576.
[15]	张永平,朱为民,崔辉梅.上海地区番茄烟粉虱传双生病毒PCR检测[J].上海农业学报, 2007, 23(4):100-101.
[16]	胡新喜,何长征,熊兴耀,等.马铃薯Y病毒研究进展[J].中国马铃薯, 2009, 23(5):293-300.
[17]	OZKAYNAK E, DEVRAN Z, KAHVECI E. Development of Turkish Potato Varieties Tolerance to Potato Virus Y and Potato Virus X[J]. Ekin Journal of Crop Breeding and Genetics, 2018, 4(1):55-59.
[18]	MANSOOR S, BRIDDON R W, ZAFAR Y, et al. Geminivirus disease complexes:an emerging threat[J]. Trends in Plant Science, 2003, 8(3):128-134.
[19]	阮涛,于云奇,包凌云,等.四川米易赛葵上粉虱传双生病毒的分子鉴定及复合侵染检测[J].植物保护学报, 2011, 38(5):419-424.
[20]	王向阳.广西胜红蓟黄脉病样中发现多种菜豆金色花叶病毒属的病毒[J].植物病理学报, 2007, 37(6):679-682.
[21]	车海彦,罗大全,杨旭光,等.海南岛番木瓜和扶桑上粉虱传双生病毒的检测及序列分析[J].中国病毒学,2005, 20(5):87-89.
[22]	赵丽玲,钟静,尹跃艳,等.云南凹头苋上分离到的2种菜豆金色花叶病毒属病毒基因组结构特征[J].植物病理学报, 2017, 47(6):738-746.
[23]	谢丽雪,张小艳,郑姗,等.福建西番莲上广东番木瓜曲叶病毒的分子鉴定[J].东南园艺, 2019, 7(5):19-22.
[24]	SONG Y S, MIN B E, HONG J S, et al. Molecular evidence supporting the confirmation of maracuja mosaic virus as a species of the genus Tobamovirus and production of an infectious cDNA transcript[J]. Archives of Virology, 2006, 151(12):2337-2348.
[25]	BROWN J K. First report of Passiflora leaf mottle disease caused by a whitefly-transmitted geminivirus in Puerto Rico[J]. Plant Disease, 1993, 77(12):1264C.
[26]	BENNETT A, AGBANDJE-MCKENNA M. Geminivirus structure and assembly[J]. Advances in Virus Research,2020, 108:1-32.
[27]	FIALLO-OLIVÉE, PAN L L, LIU S S, et al. Transmission of begomoviruses and other whitefly-borne viruses:dependence on the vector species[J]. Phytopathology,2020, 110(1):10-17.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Molecular detection and analysis of main viruses infecting Passiflora edulis planted in Zhanjiang,Guangdong Province

1. Zhanjiang Research Center, Institute of Nanfan & Seed Industry, Guangdong Academy of Sciences, Zhanjiang, Guangdong, 524000, China;

2. Zhanjiang Research Academy, Guangdong Academy of Sciences, Zhanjiang, Guangdong, 524000, China

Received Date: 2023-11-14

Rev Recd Date: 2024-01-14

Key words:

Abstract: Leaves of virus-free seedlings of Passiflora edulis ‘Qinmi 9’ and suspected diseased P. edulis plants were collected for extraction of nucleic acids. P. edulis virus detection primers were designed according to the conserved regions of P. edulis woodiness virus, P. edulis mottle virus, East Asian Passiflora virus, Telosma mosaic virus, P. edulis leaf distortion virus, Euphorbia leaf curl virus, Papaya leaf curl Guangdong virus and Cucumber mosaic virus., and the virus species in different samples were identified by using PCR method. No suspected virus fragments were amplified from the virus-free seedlings of P. edulis. A total of five possible virus fragments were amplified from suspected infected P. edulis seedling samples in the field. They were identified by sequencing as East Asian P. edulis virus,Telosma mosaic virus and Euphorbia leaf curl virus, Papaya leaf curl Guangdong virus. Sequence alignment and phylogenetic tree analysis showed that the PCR products had a high homology with the virus strains from Taiwan Province of China, the Republic of Korea, Fujian Province of China and other regions. The ‘Qinmi 9’ plant planted locally in Zhanjiang was confirmed to carry the virus EAPV, TeMV, EuLCV, PaLCuGDV. This is also the first time that viruses EAPV, PaLCuGV and EuLCV were detected in P. edulis in Guangdong, China.

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Reference (27)

[1]	田青兰,吴艳艳,黄伟华,等.'台农1号'西番莲的成花坐果特性及与气象因子的关系[J].果树学报, 2020,37(9):1358-1370.
[2]	徐平东,李梅,柯冲.福建西番莲病毒病的发生及其病原黄瓜花叶病毒亚组鉴定[J].植物保护学报,1999, 26(1):50-54.
[3]	CHANG C A. Characterization and comparison of passionfruit mottle virus, a newly recognized potyvirus, with passionfruit woodiness virus[J]. Phytopathology, 1992, 82(11):1358.
[4]	NASCIMENTO A V S, SANTANA E N, BRAZ A S K, et al. Cowpea aphid-borne mosaic virus (CABMV) is widespread in passionfruit in Brazil and causes passionfruit woodiness disease[J]. Archives of Virology, 2006, 151(9):1797-1809.
[5]	IWAI H, YAMASHITA Y, NISHI N, et al. The potyvirus associated with the dappled fruit of Passiflora edulis in Kagoshima prefecture, Japan is the third strain of the proposed new species East Asian Passiflora virus (EAPV) phylogenetically distinguished from strains of Passion fruit woodiness virus[J]. Archives of Virology, 2006, 151(4):811-818.
[6]	CHIEMSOMBAT P, PRAMMANEE S, PIPATTANAWONG N. Occurrence of Telosma mosaic virus causing passion fruit severe mosaic disease in Thailand and immunostrip test for rapid virus detection[J]. Crop Protection, 2014, 63:41-47.
[7]	CHENG Y H, DENG T C, CHEN C C, et al. First Report of Euphorbia leaf curl virus and Papaya leaf curl Guangdong virus on Passion Fruit in Taiwan[J]. Plant Disease,2014, 98(12):1746.
[8]	宋若楠,侯永翔,崔梦迪,等.广东百香果病毒的检测[J].仲恺农业工程学院学报, 2020, 33(1):11-15.
[9]	谢丽雪,张立杰,张小艳,等.福建西番莲上首次检出东亚西番莲病毒[J].园艺学报, 2018, 45(8):1587-1594.
[10]	谢丽雪,张小艳,郑姗,等.侵染西番莲的夜来香花叶病毒的分子鉴定及特异性检测[J].中国农业科学,2017, 50(24):4725-4734.
[11]	李景远.广东番木瓜曲叶病毒和大戟曲叶病毒的致病性分析[D].福州:福建农林大学, 2022.
[12]	谢慧婷,崔丽贤,李战彪,等.广西百香果病毒病病原种类鉴定研究初报[C]//中国植物病理学会.中国植物病理学会2018年学术年会论文集.中国农业科学技术出版社, 2018:1.
[13]	WANG X, XIE Y, ZHOU X. Molecular characterization of two distinct begomovirusesfrom Papaya in China[J].Virus Genes, 2004, 29(3):303-309.
[14]	VACA-VACA J C, CARRASCO-LOZANO E C, LÓPEZLÓPEZ K. Molecular identification of a new begomovirus infecting yellow passion fruit (Passiflora edulis) in Colombia[J]. Archives of Virology, 2017, 162(2):573-576.
[15]	张永平,朱为民,崔辉梅.上海地区番茄烟粉虱传双生病毒PCR检测[J].上海农业学报, 2007, 23(4):100-101.
[16]	胡新喜,何长征,熊兴耀,等.马铃薯Y病毒研究进展[J].中国马铃薯, 2009, 23(5):293-300.
[17]	OZKAYNAK E, DEVRAN Z, KAHVECI E. Development of Turkish Potato Varieties Tolerance to Potato Virus Y and Potato Virus X[J]. Ekin Journal of Crop Breeding and Genetics, 2018, 4(1):55-59.
[18]	MANSOOR S, BRIDDON R W, ZAFAR Y, et al. Geminivirus disease complexes:an emerging threat[J]. Trends in Plant Science, 2003, 8(3):128-134.
[19]	阮涛,于云奇,包凌云,等.四川米易赛葵上粉虱传双生病毒的分子鉴定及复合侵染检测[J].植物保护学报, 2011, 38(5):419-424.
[20]	王向阳.广西胜红蓟黄脉病样中发现多种菜豆金色花叶病毒属的病毒[J].植物病理学报, 2007, 37(6):679-682.
[21]	车海彦,罗大全,杨旭光,等.海南岛番木瓜和扶桑上粉虱传双生病毒的检测及序列分析[J].中国病毒学,2005, 20(5):87-89.
[22]	赵丽玲,钟静,尹跃艳,等.云南凹头苋上分离到的2种菜豆金色花叶病毒属病毒基因组结构特征[J].植物病理学报, 2017, 47(6):738-746.
[23]	谢丽雪,张小艳,郑姗,等.福建西番莲上广东番木瓜曲叶病毒的分子鉴定[J].东南园艺, 2019, 7(5):19-22.
[24]	SONG Y S, MIN B E, HONG J S, et al. Molecular evidence supporting the confirmation of maracuja mosaic virus as a species of the genus Tobamovirus and production of an infectious cDNA transcript[J]. Archives of Virology, 2006, 151(12):2337-2348.
[25]	BROWN J K. First report of Passiflora leaf mottle disease caused by a whitefly-transmitted geminivirus in Puerto Rico[J]. Plant Disease, 1993, 77(12):1264C.
[26]	BENNETT A, AGBANDJE-MCKENNA M. Geminivirus structure and assembly[J]. Advances in Virus Research,2020, 108:1-32.
[27]	FIALLO-OLIVÉE, PAN L L, LIU S S, et al. Transmission of begomoviruses and other whitefly-borne viruses:dependence on the vector species[J]. Phytopathology,2020, 110(1):10-17.

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Molecular detection and analysis of main viruses infecting Passiflora edulis planted in Zhanjiang,Guangdong Province

doi: 10.15886/j.cnki.rdswxb.20230126

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

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Molecular detection and analysis of main viruses infecting Passiflora edulis planted in Zhanjiang,Guangdong Province

doi: 10.15886/j.cnki.rdswxb.20230126

1. Zhanjiang Research Center, Institute of Nanfan & Seed Industry, Guangdong Academy of Sciences, Zhanjiang, Guangdong, 524000, China;

2. Zhanjiang Research Academy, Guangdong Academy of Sciences, Zhanjiang, Guangdong, 524000, China

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Molecular detection and analysis of main viruses infecting Passiflora edulis planted in Zhanjiang,Guangdong Province

doi: 10.15886/j.cnki.rdswxb.20230126

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

Related

Molecular detection and analysis of main viruses infecting Passiflora edulis planted in Zhanjiang,Guangdong Province

doi: 10.15886/j.cnki.rdswxb.20230126

1. Zhanjiang Research Center, Institute of Nanfan & Seed Industry, Guangdong Academy of Sciences, Zhanjiang, Guangdong, 524000, China; 2. Zhanjiang Research Academy, Guangdong Academy of Sciences, Zhanjiang, Guangdong, 524000, China

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1. Zhanjiang Research Center, Institute of Nanfan & Seed Industry, Guangdong Academy of Sciences, Zhanjiang, Guangdong, 524000, China;

2. Zhanjiang Research Academy, Guangdong Academy of Sciences, Zhanjiang, Guangdong, 524000, China