HE Mengxue, LI Zeyou. Extraction and SDS-PAGE Electrophoresis of Anticoagulant Components from Poecilobdella manillensis Lesson in Hainan[J]. Journal of Tropical Biology, 2019, 10(2): 204-207. doi: 10.15886/j.cnki.rdswxb.2019.02.017
| Citation:
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HE Mengxue, LI Zeyou. Extraction and SDS-PAGE Electrophoresis of Anticoagulant Components from Poecilobdella manillensis Lesson in Hainan[J]. Journal of Tropical Biology, 2019, 10(2): 204-207. doi: 10.15886/j.cnki.rdswxb.2019.02.017
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Extraction and SDS-PAGE Electrophoresis of Anticoagulant Components from Poecilobdella manillensis Lesson in Hainan
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School of Pharmaceutical Sciences, Hainan Medical University, Haikou, Hainan 571199, China
- Received Date: 2019-03-20
- Rev Recd Date:
2019-05-22
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Abstract
To study the anticoagulant activity and purify the anticoagulation components from P. manillensis in Hainan. Poecilobdella manillensis Lession collected in Hainan was extracted with different extraction methods and purified with molecular weight ultra membranes to determine its anticoagulant components and activities by using the thrombin titration method. The relative molecular weight of the anticoagulant components was calculated by using the SDS-PAGE test. The results showed that the sodium chloride solution was better than the water extraction, the water extraction and alcohol precipitation, and the acetone extract in extraction of anticoagulant components. The anticoagulant activity of the extract from the head of P. manillensis extracted by the sodium chloride solution was 1 811.11 U·g-1, the highest among the other extraction methods. The ultra-membranes with molecular weights of 10×103 and 30×103 were selected for purification, and the molecular weight of the purified protein was 15.96×103.
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