Identification and Expression Analysis of MKK Genes in <i>Cassava</i>

HOU Pengyu; YU Xinyi; XIAO Xiaorong; ZHENG Linlin; CHEN Yinhua

doi:10.15886/j.cnki.rdswxb.2019.02.004

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Volume 10 Issue 2

Jun. 2019

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Article Navigation > Journal of Tropical Biology > 2019 > 10(2): 119-126

HOU Pengyu, YU Xinyi, XIAO Xiaorong, ZHENG Linlin, CHEN Yinhua. Identification and Expression Analysis of MKK Genes in Cassava[J]. Journal of Tropical Biology, 2019, 10(2): 119-126. doi: 10.15886/j.cnki.rdswxb.2019.02.004

Citation:

HOU Pengyu, YU Xinyi, XIAO Xiaorong, ZHENG Linlin, CHEN Yinhua. Identification and Expression Analysis of MKK Genes in Cassava[J]. Journal of Tropical Biology, 2019, 10(2): 119-126. doi: 10.15886/j.cnki.rdswxb.2019.02.004

Identification and Expression Analysis of MKK Genes in Cassava

doi: 10.15886/j.cnki.rdswxb.2019.02.004

Institute of Tropical Agriculture and Forestry, Hainan University/Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Haikou, Hainan 570228, China

Received Date: 2019-01-28
Rev Recd Date: 2019-02-27

Abstract

The mitogen-activated protein kinase（MAPK） cascade pathway is a signaling pathway widely present in eukaryotic organisms. Based on cassava genomic data, a comprehensive identification and phylogenetic tree analysis of mitogen-activated protein kinase kinase kinase（MKK） family members in cassava was carried out by bioinformatics methods. At the same time, the expression pattern of each gene of the MKK family was analyzed through hormone treatment and pathogen inoculation. The results showed that cassava encoded 11 MKK genes, which were distributed on chromosomes 3, 4, 6, 10, 12, 16, and 17 of cassava. The expression analysis showed that MKKs were responsive to ABA, JA and pathogen signal but not sensitive to ACC signal, of which MKK4, MKK5, MKK8, MKK9 and MKK11 may be involved in cassava-related pathogens defense pathways and hormone signaling pathways.
- Cassava,
- MKK gene,
- identification,
- expression analysis

References

[1]	PITZSCHKE A, SCHIKORA A, HIRT H.MAPK cascade signalling networks in plant defence[J].Current Opinion in Plant Biology, 2009, 12 (4) 421-426.
[2]	MENG X Z, ZHANG S Q.MAPK cascades in plant disease resistance signaling[J].Annual Review of Phytopathology, 2013, 51:245-266.
[3]	FULING K, JIE W, LIN C, et al.Genome-wide analysis of the mitogen-activated protein kinase gene family in Solanum lycopersicum[J].Gene, 2012, 499 (1) 108-120.
[4]	NAKAGAMI H, PITZSCHKE A, HIRT H.Emerging MAP kinase pathways in plant stress signaling[J].Trends in Plant Science, 2005, 10 (7) 339-346.
[5]	DANQUAH A, ZELICOURT A, COLCOMBET J, et al.The role of ABA and MAPK signaling pathways in plant abiotic stress responses[J].Biotechnology Advances, 2014, 32 (1):40-52.
[6]	YANG T B, CHAUDHURI S, YANG L H, et al.A calcium/calmodulin-regulated member of the receptor-like kinase family confers cold tolerance in plants[J].JBC Papers in Press, 2009, 285 (10):7119-7126.
[7]	HAN S, WANG C W, WANG W L, et al.Mitogen-activated protein kinase 6 controls root growth in Arabidopsis by modulating Ca2+-based Na⁺ flux in root cell under salt stress[J].Journal of plant physiology, 2014, 171 (5):26-34.
[8]	SUN H K, DONG H W, JAE M K, et al.Arabidopsis MKK4 mediates osmotic-stress response via its regulation of MPK3 activity[J].Biochemical and Biophysical Research Communications, 2011, 412 (1):150-154.
[9]	LIU Y D, REN D T, SHARON P, et al.Chloroplast-generated reactive oxygen species are involved in hypersensitive response-like cell death mediated by a mitogen-activated protein kinase cascade[J].The Plant Journal, 2007, 51 (6):941-954.
[10]	MCDOWELL M J, JEFFERY L D.Signal transduction in the plant immune response[J].Trends in Biochemical Sciences, 2000, 25 (2):79-82.
[11]	KATHY M, DONG H, JÊRÔME G, et al.Transcript profiling in Vitis riparia during chilling requirement fulfillment reveals coordination of gene expression patterns with optimized bud break[J].Functional & Integrative Genomics, 2009, 9 (1):81-96.
[12]	JAI S.R, YANG Y N.Rice Mitogen-activated Protein Kinase Gene Family and Its Role in Biotic and Abiotic Stress Response[J].Journal of Integrative Plant Biology, 2007, 49 (6):751-759.
[13]	LEI G and CHENG B X.The genetic locus At1g73660 encodes a putative MAPKKK and negatively regulates salt tolerance in Arabidopsis[J].Plant Molecular Biology, 2008, 67 (1/2):125-134.
[14]	HAMEL L P, NICOLE M C, SRITUBTIM S, et al.Ancient signals:comparative genomics of plant MAPK and MAPKK gene families[J].Trends in Plant Science, 2006, 11 (4):192-198.
[15]	SONG Q M, LI D Y, DAI Y, et al.Characterization, expression patterns and functional analysis of the MAPK and MAPKK genes in watermelon (Citrullus lanatus) [J].BMC Plant Biology, 2015, 15 (1):298.
[16]	REN D, YANG K Y, LI G J, et al.Activation of Ntf4, a tobacco mitogen-activated protein kinase, during plant defense response and its involvement in hypersensitive response-like cell death[J].Plant Physiology, 2006, 141 (4):1482-1493.
[17]	WANG J, PAN C T, WANG Y, et al.Genome-wide identification of MAPK, MAPKK, and MAPKKK gene families and transcriptional profiling analysis during development and stress response in cucumber[J].BMC Genomics, 2015, 16 (1):386-402.
[18]	ASAI T, TENA G, PLOTNIKOVA J, et al.MAP kinase signaling cascade in Arabidopsis innate immunity[J].Nature, 2002, 415 (6875):977-983.
[19]	JIA W Y, LI B H, LI S J, et al.Mitogen-activated protein kinase cascade MKK7-MPK6 plays important roles in plant development and regulates shoot branching by phosphorylating PIN1 in Arabidopsis[J].PLoS Biology, 2016, 14 (9):e1002550.
[20]	ZHOU C J, CAI Z H, GUO Y F, et al.An arabidopsis mitogen-activated protein kinase cascade, MKK9-MPK6, plays a role in leaf senescence[J].Plant Physiology, 2009, 150 (1):167-177.
[21]	WANG H C, NGWENYAMA N, LIU Y D, et al.Stomatal development and patterning are regulated by environmentally responsive mitogen-activated protein kinases in Arabidopsis[J].The Plant Cell, 2007, 19 (1):63-73.
[22]	SUAREZ R M C, ADAMS P P L, LIU Y D, et al.MEKK1 is required for flg22-induced MPK4 activation in Arabidopsis plants[J].Plant Physiology, 2006, 143 (2):661-669.
[23]	ZHANG X T, CHENG T C, WANG G H, et al.Cloning and evolutionary analysis of tobacco MAPK gene family[J].Molecular Biology Reports, 2013, 40 (2):1407-1415.
[24]	ZHANG T, CHEN S X, HARMON A C.Protein-protein interactions in plant mitogen-activated protein kinase cascades[J].Journal of Experimental Botany, 2016, 67 (3):607-618.
[25]	KONG X P, PAN J W, ZHANG D, et al.Identification of mitogen-activated protein kinase kinase gene family and MKK-MAPK interaction network in maize[J].Biochemical and Biophysical Research Communications, 2013, 441 (4):964-969.
[26]	MARKUS T, ELISABETH S, THOMAS E, et al.The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis[J].Molecular Cell, 2004, 15 (1):141-152.
[27]	KONG X P, PAN J W, ZHANG D, et al.Identification of mitogen-activated protein kinase kinase gene family and MKK-MAPK interaction network in maize[J].Biochemical and Biophysical Research Communications, 2013, 441 (4):964-969.
[28]	HAMEL L P, NICOLE M C, SRITUBTIM S, et al.Ancient signals:comparative genomics of plant MAPK and MAPKK gene families[J].Trends Plant Sci, 2006, 11 (4):192-198.
[29]	SINHA A K, JAGGI M, RAGHURAM B, et al.Mitogen-activated protein kinase signaling in plants under abiotic stress[J].Plant Signal Behav, 2011, 6 (2):196-203.
[30]	ZHAO L, WANG C, ZHU F, et al.Mild osmotic stress promotes 4-methoxy indolyl-3-methyl glucosinolate biosynthesis mediated by the MKK9-MPK3/MPK6 cascade in Arabidopsis[J].Plant Cell Rep, 2017, 36 (4):543-555.
[31]	LIU Z, LI Y, CAO H, et al.Comparative phospho-proteomics analysis of salt-responsive phosphoproteins regulated by the MKK9-MPK6 cascade in Arabidopsis[J].Plant Sci, 2015, 241:138-150.
[32]	WU D Y, JI J, WANG G, et al.LcMKK, a novel group A mitogen-activated protein kinase kinase gene in Lycium chinense, confers dehydration and drought tolerance in transgenic tobacco via scavenging ROS and modulating expression of stress-responsive genes[J].Plant Growth Regulation, 2015, 76 (3):269-279.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Identification and Expression Analysis of MKK Genes in Cassava

Institute of Tropical Agriculture and Forestry, Hainan University/Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Haikou, Hainan 570228, China

Received Date: 2019-01-28

Rev Recd Date: 2019-02-27

Key words:

Abstract: The mitogen-activated protein kinase（MAPK） cascade pathway is a signaling pathway widely present in eukaryotic organisms. Based on cassava genomic data, a comprehensive identification and phylogenetic tree analysis of mitogen-activated protein kinase kinase kinase（MKK） family members in cassava was carried out by bioinformatics methods. At the same time, the expression pattern of each gene of the MKK family was analyzed through hormone treatment and pathogen inoculation. The results showed that cassava encoded 11 MKK genes, which were distributed on chromosomes 3, 4, 6, 10, 12, 16, and 17 of cassava. The expression analysis showed that MKKs were responsive to ABA, JA and pathogen signal but not sensitive to ACC signal, of which MKK4, MKK5, MKK8, MKK9 and MKK11 may be involved in cassava-related pathogens defense pathways and hormone signaling pathways.

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Reference (32)

[1]	PITZSCHKE A, SCHIKORA A, HIRT H.MAPK cascade signalling networks in plant defence[J].Current Opinion in Plant Biology, 2009, 12 (4) 421-426.
[2]	MENG X Z, ZHANG S Q.MAPK cascades in plant disease resistance signaling[J].Annual Review of Phytopathology, 2013, 51:245-266.
[3]	FULING K, JIE W, LIN C, et al.Genome-wide analysis of the mitogen-activated protein kinase gene family in Solanum lycopersicum[J].Gene, 2012, 499 (1) 108-120.
[4]	NAKAGAMI H, PITZSCHKE A, HIRT H.Emerging MAP kinase pathways in plant stress signaling[J].Trends in Plant Science, 2005, 10 (7) 339-346.
[5]	DANQUAH A, ZELICOURT A, COLCOMBET J, et al.The role of ABA and MAPK signaling pathways in plant abiotic stress responses[J].Biotechnology Advances, 2014, 32 (1):40-52.
[6]	YANG T B, CHAUDHURI S, YANG L H, et al.A calcium/calmodulin-regulated member of the receptor-like kinase family confers cold tolerance in plants[J].JBC Papers in Press, 2009, 285 (10):7119-7126.
[7]	HAN S, WANG C W, WANG W L, et al.Mitogen-activated protein kinase 6 controls root growth in Arabidopsis by modulating Ca2+-based Na⁺ flux in root cell under salt stress[J].Journal of plant physiology, 2014, 171 (5):26-34.
[8]	SUN H K, DONG H W, JAE M K, et al.Arabidopsis MKK4 mediates osmotic-stress response via its regulation of MPK3 activity[J].Biochemical and Biophysical Research Communications, 2011, 412 (1):150-154.
[9]	LIU Y D, REN D T, SHARON P, et al.Chloroplast-generated reactive oxygen species are involved in hypersensitive response-like cell death mediated by a mitogen-activated protein kinase cascade[J].The Plant Journal, 2007, 51 (6):941-954.
[10]	MCDOWELL M J, JEFFERY L D.Signal transduction in the plant immune response[J].Trends in Biochemical Sciences, 2000, 25 (2):79-82.
[11]	KATHY M, DONG H, JÊRÔME G, et al.Transcript profiling in Vitis riparia during chilling requirement fulfillment reveals coordination of gene expression patterns with optimized bud break[J].Functional & Integrative Genomics, 2009, 9 (1):81-96.
[12]	JAI S.R, YANG Y N.Rice Mitogen-activated Protein Kinase Gene Family and Its Role in Biotic and Abiotic Stress Response[J].Journal of Integrative Plant Biology, 2007, 49 (6):751-759.
[13]	LEI G and CHENG B X.The genetic locus At1g73660 encodes a putative MAPKKK and negatively regulates salt tolerance in Arabidopsis[J].Plant Molecular Biology, 2008, 67 (1/2):125-134.
[14]	HAMEL L P, NICOLE M C, SRITUBTIM S, et al.Ancient signals:comparative genomics of plant MAPK and MAPKK gene families[J].Trends in Plant Science, 2006, 11 (4):192-198.
[15]	SONG Q M, LI D Y, DAI Y, et al.Characterization, expression patterns and functional analysis of the MAPK and MAPKK genes in watermelon (Citrullus lanatus) [J].BMC Plant Biology, 2015, 15 (1):298.
[16]	REN D, YANG K Y, LI G J, et al.Activation of Ntf4, a tobacco mitogen-activated protein kinase, during plant defense response and its involvement in hypersensitive response-like cell death[J].Plant Physiology, 2006, 141 (4):1482-1493.
[17]	WANG J, PAN C T, WANG Y, et al.Genome-wide identification of MAPK, MAPKK, and MAPKKK gene families and transcriptional profiling analysis during development and stress response in cucumber[J].BMC Genomics, 2015, 16 (1):386-402.
[18]	ASAI T, TENA G, PLOTNIKOVA J, et al.MAP kinase signaling cascade in Arabidopsis innate immunity[J].Nature, 2002, 415 (6875):977-983.
[19]	JIA W Y, LI B H, LI S J, et al.Mitogen-activated protein kinase cascade MKK7-MPK6 plays important roles in plant development and regulates shoot branching by phosphorylating PIN1 in Arabidopsis[J].PLoS Biology, 2016, 14 (9):e1002550.
[20]	ZHOU C J, CAI Z H, GUO Y F, et al.An arabidopsis mitogen-activated protein kinase cascade, MKK9-MPK6, plays a role in leaf senescence[J].Plant Physiology, 2009, 150 (1):167-177.
[21]	WANG H C, NGWENYAMA N, LIU Y D, et al.Stomatal development and patterning are regulated by environmentally responsive mitogen-activated protein kinases in Arabidopsis[J].The Plant Cell, 2007, 19 (1):63-73.
[22]	SUAREZ R M C, ADAMS P P L, LIU Y D, et al.MEKK1 is required for flg22-induced MPK4 activation in Arabidopsis plants[J].Plant Physiology, 2006, 143 (2):661-669.
[23]	ZHANG X T, CHENG T C, WANG G H, et al.Cloning and evolutionary analysis of tobacco MAPK gene family[J].Molecular Biology Reports, 2013, 40 (2):1407-1415.
[24]	ZHANG T, CHEN S X, HARMON A C.Protein-protein interactions in plant mitogen-activated protein kinase cascades[J].Journal of Experimental Botany, 2016, 67 (3):607-618.
[25]	KONG X P, PAN J W, ZHANG D, et al.Identification of mitogen-activated protein kinase kinase gene family and MKK-MAPK interaction network in maize[J].Biochemical and Biophysical Research Communications, 2013, 441 (4):964-969.
[26]	MARKUS T, ELISABETH S, THOMAS E, et al.The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis[J].Molecular Cell, 2004, 15 (1):141-152.
[27]	KONG X P, PAN J W, ZHANG D, et al.Identification of mitogen-activated protein kinase kinase gene family and MKK-MAPK interaction network in maize[J].Biochemical and Biophysical Research Communications, 2013, 441 (4):964-969.
[28]	HAMEL L P, NICOLE M C, SRITUBTIM S, et al.Ancient signals:comparative genomics of plant MAPK and MAPKK gene families[J].Trends Plant Sci, 2006, 11 (4):192-198.
[29]	SINHA A K, JAGGI M, RAGHURAM B, et al.Mitogen-activated protein kinase signaling in plants under abiotic stress[J].Plant Signal Behav, 2011, 6 (2):196-203.
[30]	ZHAO L, WANG C, ZHU F, et al.Mild osmotic stress promotes 4-methoxy indolyl-3-methyl glucosinolate biosynthesis mediated by the MKK9-MPK3/MPK6 cascade in Arabidopsis[J].Plant Cell Rep, 2017, 36 (4):543-555.
[31]	LIU Z, LI Y, CAO H, et al.Comparative phospho-proteomics analysis of salt-responsive phosphoproteins regulated by the MKK9-MPK6 cascade in Arabidopsis[J].Plant Sci, 2015, 241:138-150.
[32]	WU D Y, JI J, WANG G, et al.LcMKK, a novel group A mitogen-activated protein kinase kinase gene in Lycium chinense, confers dehydration and drought tolerance in transgenic tobacco via scavenging ROS and modulating expression of stress-responsive genes[J].Plant Growth Regulation, 2015, 76 (3):269-279.

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Identification and Expression Analysis of MKK Genes in Cassava

doi: 10.15886/j.cnki.rdswxb.2019.02.004

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

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Identification and Expression Analysis of MKK Genes in Cassava

doi: 10.15886/j.cnki.rdswxb.2019.02.004

Institute of Tropical Agriculture and Forestry, Hainan University/Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Haikou, Hainan 570228, China

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Identification and Expression Analysis of MKK Genes in Cassava

doi: 10.15886/j.cnki.rdswxb.2019.02.004

Abstract

References

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通讯作者: 陈斌, bchen63@163.com

Proportional views

Related

Identification and Expression Analysis of MKK Genes in Cassava

doi: 10.15886/j.cnki.rdswxb.2019.02.004

Institute of Tropical Agriculture and Forestry, Hainan University/Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Haikou, Hainan 570228, China

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