Isolation and Identification of a Multiple Resistant Bacterium to Heavy Metals

NIU Shiyao; LIU Peiyi; XU Yufeng; LONG Yunpiao; NIU Xiaolei; LI Chunxia; CHEN Yinhua; TAO Jun

doi:10.15886/j.cnki.rdswxb.2019.01.007

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Heavy metals could affect the development of plants and microbial communities in soil. They can be transported into plants and animals by the food chain and may cause human diseases. In this context soil samples were collected in Changjiang mining area of Hainan Province, China, from which four copper-resistant strains were isolated. These strains were cultured in the LB medium containing different concentrations of heavy metals, and found to be resistant to copper. Moreover, the strain Cu-6 was observed to be resistant to plumbum（Pb） and cadmium（Cd） as well. Cu-6 was identified as Enterobacter cloacae, and had an optimal growth in the conditions of 34 ℃, pH6.0 and 0.5% NaCl. Cu-6 showed a good growth in the liquid LB medium containing 800 mg·L^-1 CuSO₄·5 H₂O, 1 700 mg·L^-1 C₄H₆O₄Pb·3 H₂O, 150 mg·L^-1 CdCl₂·2.5 H₂O, or even mixture of these three metal ions, indicating that this strain should have a strong adaptation to the complex soil environment, and have a potential of soil improvement from the soil contaminated by heavy metals.

Isolation and Identification of a Multiple Resistant Bacterium to Heavy Metals

Institute of Tropical Agriculture and Forestry, Hainan University;Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Haikou, Hainan 570228, China

Received Date: 2018-10-09

Rev Recd Date: 2018-11-12

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Abstract: Heavy metals could affect the development of plants and microbial communities in soil. They can be transported into plants and animals by the food chain and may cause human diseases. In this context soil samples were collected in Changjiang mining area of Hainan Province, China, from which four copper-resistant strains were isolated. These strains were cultured in the LB medium containing different concentrations of heavy metals, and found to be resistant to copper. Moreover, the strain Cu-6 was observed to be resistant to plumbum（Pb） and cadmium（Cd） as well. Cu-6 was identified as Enterobacter cloacae, and had an optimal growth in the conditions of 34 ℃, pH6.0 and 0.5% NaCl. Cu-6 showed a good growth in the liquid LB medium containing 800 mg·L^-1 CuSO₄·5 H₂O, 1 700 mg·L^-1 C₄H₆O₄Pb·3 H₂O, 150 mg·L^-1 CdCl₂·2.5 H₂O, or even mixture of these three metal ions, indicating that this strain should have a strong adaptation to the complex soil environment, and have a potential of soil improvement from the soil contaminated by heavy metals.

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Reference (18)

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Isolation and Identification of a Multiple Resistant Bacterium to Heavy Metals

doi: 10.15886/j.cnki.rdswxb.2019.01.007

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