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Volume 10 Issue 1
Mar.  2019
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Article Navigation >  Journal of Tropical Biology  > 2019  >  10(1): 6-13

CHEN Jinhao, ZHENG Jinbin, MAO Yong, SU Yongquan, WANG Jun. Effects of Probiotics and Compound Nutrients on Water Quality and Community Structure of Bacteria in Fish-shrimp Farming Pond[J]. Journal of Tropical Biology, 2019, 10(1): 6-13. doi: 10.15886/j.cnki.rdswxb.2019.01.002
Citation: CHEN Jinhao, ZHENG Jinbin, MAO Yong, SU Yongquan, WANG Jun. Effects of Probiotics and Compound Nutrients on Water Quality and Community Structure of Bacteria in Fish-shrimp Farming Pond[J]. Journal of Tropical Biology, 2019, 10(1): 6-13. doi: 10.15886/j.cnki.rdswxb.2019.01.002
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Effects of Probiotics and Compound Nutrients on Water Quality and Community Structure of Bacteria in Fish-shrimp Farming Pond

doi: 10.15886/j.cnki.rdswxb.2019.01.002

  • 1. College of Ocean And Earth Sciences, Xiamen University, Xiamen, Fujian 361005, China;

  • 2. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, Fujian 361005, China

  • Received Date: 2018-08-03
  • Rev Recd Date: 2018-12-19
  • Probiotics and compound nutrients were added into a fish-shrimp mix-culturing pond to regulate the aquaculture environment in the pond, and their effect on water quality was evaluated. The bacterial diversity in the water of the mix-culturing pond was estimated via a high throughput 16 S rDNA sequencing by using Illumina Instrument HiSeq2500 and its composition and abundance were analyzed. The water quality test showed that the water quality parameters in the mix-culturing ponds, such as transparency, pH, dissolved oxygen（DO）, ammonia nitrogen（NH3-N） and nitrite（NO2-N）, fell within the appropriate range of fish and shrimp farming standard and that the concentration of harmful vibrio bacteria was far lower than the pathogenic concentration, which indicates that this method can maintain stable water environment and inhibit the reproduction of pathogenic bacteria. The analysis of bacterial community composition and abundance showed that the number of bacterial OTU in the aquaculture water was 1878, among which the number of OTU in kingdom, phylum, class, order, family, genus and species was 4, 43, 79, 91, 652, 952 and 16, respectively. The relative abundance of bacteria in the aquaculture water was mainly dominated by Cyanobacteria, Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes, accounting for 38.19%, 28.11%, 18.85%, 12.85% and 0.08% of the total bacteria, respectively. The result indicates that the water in fish-shrimp mix-culturing pond had a higher diversity of bacteria in the bacterial community after the probiotics and compound nutrients were added into the mix-culturing pond, and contained many common probiotics. This study provides a theoretical basis for improving the ecological environment of aquaculture and healthy farming of fish and shrimp.
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Effects of Probiotics and Compound Nutrients on Water Quality and Community Structure of Bacteria in Fish-shrimp Farming Pond

doi: 10.15886/j.cnki.rdswxb.2019.01.002
  • 1. College of Ocean And Earth Sciences, Xiamen University, Xiamen, Fujian 361005, China;
  • 2. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, Fujian 361005, China
  • Received Date: 2018-08-03
  • Rev Recd Date: 2018-12-19

Abstract: Probiotics and compound nutrients were added into a fish-shrimp mix-culturing pond to regulate the aquaculture environment in the pond, and their effect on water quality was evaluated. The bacterial diversity in the water of the mix-culturing pond was estimated via a high throughput 16 S rDNA sequencing by using Illumina Instrument HiSeq2500 and its composition and abundance were analyzed. The water quality test showed that the water quality parameters in the mix-culturing ponds, such as transparency, pH, dissolved oxygen（DO）, ammonia nitrogen（NH3-N） and nitrite（NO2-N）, fell within the appropriate range of fish and shrimp farming standard and that the concentration of harmful vibrio bacteria was far lower than the pathogenic concentration, which indicates that this method can maintain stable water environment and inhibit the reproduction of pathogenic bacteria. The analysis of bacterial community composition and abundance showed that the number of bacterial OTU in the aquaculture water was 1878, among which the number of OTU in kingdom, phylum, class, order, family, genus and species was 4, 43, 79, 91, 652, 952 and 16, respectively. The relative abundance of bacteria in the aquaculture water was mainly dominated by Cyanobacteria, Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes, accounting for 38.19%, 28.11%, 18.85%, 12.85% and 0.08% of the total bacteria, respectively. The result indicates that the water in fish-shrimp mix-culturing pond had a higher diversity of bacteria in the bacterial community after the probiotics and compound nutrients were added into the mix-culturing pond, and contained many common probiotics. This study provides a theoretical basis for improving the ecological environment of aquaculture and healthy farming of fish and shrimp.

CHEN Jinhao, ZHENG Jinbin, MAO Yong, SU Yongquan, WANG Jun. Effects of Probiotics and Compound Nutrients on Water Quality and Community Structure of Bacteria in Fish-shrimp Farming Pond[J]. Journal of Tropical Biology, 2019, 10(1): 6-13. doi: 10.15886/j.cnki.rdswxb.2019.01.002
Citation: CHEN Jinhao, ZHENG Jinbin, MAO Yong, SU Yongquan, WANG Jun. Effects of Probiotics and Compound Nutrients on Water Quality and Community Structure of Bacteria in Fish-shrimp Farming Pond[J]. Journal of Tropical Biology, 2019, 10(1): 6-13. doi: 10.15886/j.cnki.rdswxb.2019.01.002
shu

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