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Volume 9 Issue 4
Dec.  2018
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Article Navigation >  Journal of Tropical Biology  > 2018  >  9(4): 464-468

LI Yang, YANG Ying, ZHANG Linyun, DENG Shiming. Improvement of the Traditional Preparation Process and Determination of Polypeptide Content of a Li Ethnic Group Medicine: Sangonggao[J]. Journal of Tropical Biology, 2018, 9(4): 464-468. doi: 10.15886/j.cnki.rdswxb.2018.04.016
Citation: LI Yang, YANG Ying, ZHANG Linyun, DENG Shiming. Improvement of the Traditional Preparation Process and Determination of Polypeptide Content of a Li Ethnic Group Medicine: Sangonggao[J]. Journal of Tropical Biology, 2018, 9(4): 464-468. doi: 10.15886/j.cnki.rdswxb.2018.04.016
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Improvement of the Traditional Preparation Process and Determination of Polypeptide Content of a Li Ethnic Group Medicine: Sangonggao

doi: 10.15886/j.cnki.rdswxb.2018.04.016

  • 1. College of Ocean, Hainan University, Haikou, Hainan 570228, China;

  • 2. Ministry of Education Key Laboratory of Tropical Biological Resources, Hainan University, Haikou, Hainan 570228, China

  • Received Date: 2018-03-23
  • Rev Recd Date: 2018-08-30
  • Sangonggao is a popular traditional healthcare medicine prepared by the Hainan Li Ethnic Group,and its preparation is time consuming and less effective. An enzymatic hydrolysis was used to improve the traditional preparation process of Sangonggao,and the polypeptide content of Sangonggao was determined by employing the Coomassie blue staining method or the biuret method. The results showed that the preparation process of Sangongao was improved through additional steps such as glycolysis,centrifugation and removal of fats and oils in the basis of the traditional preparation process. With this improved method the preparation was shortened from 5days and 4 nights to about 2 days and the Sangonggao paste yield increased from about 3. 0% to 8. 84%; the polypeptide content of the prepared Sangonggao was raised from 11. 90 % to 40. 36 %. The biuret method was found to be simple and accurate in determination of polypeptide content in Sangonggao.
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Improvement of the Traditional Preparation Process and Determination of Polypeptide Content of a Li Ethnic Group Medicine: Sangonggao

doi: 10.15886/j.cnki.rdswxb.2018.04.016
  • 1. College of Ocean, Hainan University, Haikou, Hainan 570228, China;
  • 2. Ministry of Education Key Laboratory of Tropical Biological Resources, Hainan University, Haikou, Hainan 570228, China
  • Received Date: 2018-03-23
  • Rev Recd Date: 2018-08-30

Abstract: Sangonggao is a popular traditional healthcare medicine prepared by the Hainan Li Ethnic Group,and its preparation is time consuming and less effective. An enzymatic hydrolysis was used to improve the traditional preparation process of Sangonggao,and the polypeptide content of Sangonggao was determined by employing the Coomassie blue staining method or the biuret method. The results showed that the preparation process of Sangongao was improved through additional steps such as glycolysis,centrifugation and removal of fats and oils in the basis of the traditional preparation process. With this improved method the preparation was shortened from 5days and 4 nights to about 2 days and the Sangonggao paste yield increased from about 3. 0% to 8. 84%; the polypeptide content of the prepared Sangonggao was raised from 11. 90 % to 40. 36 %. The biuret method was found to be simple and accurate in determination of polypeptide content in Sangonggao.

LI Yang, YANG Ying, ZHANG Linyun, DENG Shiming. Improvement of the Traditional Preparation Process and Determination of Polypeptide Content of a Li Ethnic Group Medicine: Sangonggao[J]. Journal of Tropical Biology, 2018, 9(4): 464-468. doi: 10.15886/j.cnki.rdswxb.2018.04.016
Citation: LI Yang, YANG Ying, ZHANG Linyun, DENG Shiming. Improvement of the Traditional Preparation Process and Determination of Polypeptide Content of a Li Ethnic Group Medicine: Sangonggao[J]. Journal of Tropical Biology, 2018, 9(4): 464-468. doi: 10.15886/j.cnki.rdswxb.2018.04.016
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