Screening and Identification of Endophytic Fungi Favorable for the Growth of Anoectochilus formosanus（Orchidaceae）

Key words: 
• Anoectochilus formosanus /
• growth-promoting fungus /
• molecular identification /
• medicinal orchid

Abstract: Endophytic fungi which can promote the growth and development of Anoectochilus formosanus were selected and used for natural protection and artificial cultivation of Anoectochilus formosanus. Anoectochilus formosanus was collected from Bawangling,Hainan province and cultured to isolate the endophytic fungi from their roots through symbiotic culture,and effective strains of endophytic fungi were isolated and used to promote growth and development of A. formosanus. The results showed that total 36 endophytic fungi were isolated from A. formosanus,of which 5 strains promoted the growth of the plants of A. formosanus. Of the 5 strains the strain LD02 significantly improved the fresh weight,rhizogenesis,fresh root length and the number of shoots; the strain LD16 significantly promoted rhizogenesis; the strain LD07 significantly promoted the fresh root length. Therefore,the strain LD02 could significantly promote the overall growth of A. formosanus and was hence used as the best growth-promoting endophytic fungus. The 5 strains of the growth-promoting endophytic fungi were identified by the ITS sequence. The results showed that the strain LD01 was identified as A. formosanus sp.,the strain LD02 as Tulasnella sp.,the strain LD07 as Aspergillus sp.,and the strains LD12 and LD16 as Fusarium sp.